Characterization of endemic Shigella boydii strains isolated in Iran by serotyping, antimicrobial resistance, plasmid profile, ribotyping and pulsed-field gel electrophoresis

BackgroundHaemophilus parasuisis a common porcine respiratory pathogen that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored byH. parasuisfrom pig farms in China.MethodsWe screened 143H. parasuisisolates for antimicrobial susceptibility against six fluoroquinolone antibiotics testing by the broth microdilution method, and the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrAandgyrB) and topoisomerase IV (parCandparE). The genetic relatedness among the strains was analyzed by pulsed-field gel electrophoresis.ResultsSusceptibility test showed that all isolates were low resistance to lomefloxacin (28.67%), levofloxacin (20.28%), norfloxacin (22.38%), ciprofloxacin (23.78%), however, high resistance levels were found to nalidixic acid (82.52%) and enrofloxacin (55.94%). In addition, we found 14 antimicrobial resistance genes were present in these isolates, includingblaTEM-1, blaROB-1,ermB, ermA, flor, catl, tetB, tetC, rmtB, rmtD, aadA1, aac(3′)-llc, sul1, and sul2genes. Interestingly, one isolate carried five antibiotic resistance genes (tetB, tetC, flor, rmtB, sul1). The genestetB,rmtB,andflorwere the most prevalent resistance genes inH. parasuisin China. Alterations in thegyrAgene (S83F/Y, D87Y/N/H/G) were detected in 81% of the strains andparCmutations were often accompanied by agyrAmutation. Pulsed-field gel electrophoresis typing revealed 51 unique patterns in the isolates carrying high-level antibiotic resistance genes, indicating considerable genetic diversity and suggesting that the genes were spread horizontally.DiscussionThe current study demonstrated that the high antibiotic resistance ofH. parasuisin piglets is a combination of transferable antibiotic resistance genes and multiple target gene mutations. These data provide novel insights for the better understanding of the prevalence and epidemiology of antimicrobial resistance inH. parasuis.

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Antimicrobial resistance, plasmid, virulence, multilocus sequence typing and pulsed-field gel electrophoresis profiles of Salmonella enterica serovar Typhimurium clinical and environmental isolates from India

PLoS ONE ◽

10.1371/journal.pone.0207954 ◽

2018 ◽

Vol 13 (12) ◽

pp. e0207954 ◽

Cited By ~ 8

Author(s):

Priyanka Jain ◽

Sudhanthiramani Sudhanthirakodi ◽

Goutam Chowdhury ◽

Sangeeta Joshi ◽

Shalini Anandan ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Gel Electrophoresis ◽

Multilocus Sequence Typing ◽

Salmonella Enterica ◽

Salmonella Enterica Serovar Typhimurium ◽

Pulsed Field Gel Electrophoresis ◽

Environmental Isolates ◽

Pulsed Field ◽

Resistance Plasmid ◽

Serovar Typhimurium

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Detection of three distinct genetic lineages in methicillin-resistantStaphylococcus aureus(MRSA) isolates from animals and veterinary personnel

Epidemiology and Infection ◽

10.1017/s0950268809991580 ◽

2010 ◽

Vol 138 (5) ◽

pp. 764-771 ◽

Cited By ~ 14

Author(s):

Y. ABBOTT ◽

F. C. LEONARD ◽

B. K. MARKEY

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Resistance ◽

Molecular Characterization ◽

Gel Electrophoresis ◽

Pulsed Field Gel Electrophoresis ◽

Methicillin Resistant ◽

Pulsed Field ◽

Genetic Lineages ◽

The Third

SUMMARYThis study involved the phenotypic and molecular characterization of a population of methicillin-resistantStaphylococcus aureusisolates from animals and from veterinary personnel in Ireland. Isolates from 77 animals (dogs,n=44; cats,n=4; horses,n=29) and from 28 veterinary personnel were characterized using their antimicrobial resistance profiles and pulsed-field gel electrophoresis patterns. In addition, a representative number of these isolates (n=52) were further analysed usingspa-typing techniques. The results obtained identified the presence of three distinct clonal complexes, CC5, CC8 and CC22, in both animal and human isolates. Two of these clonal complexes, CC8 and CC22, respectively, have been previously described in animals in Ireland but the presence of the third complex CC5 is a novel finding. The significance of this development, in relation to human and animal healthcare, is discussed.

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Characterization of Listeria monocytogenes on Retail Food from Jiangsu Province, China

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.1332 ◽

2013 ◽

Vol 781-784 ◽

pp. 1332-1335

Author(s):

Tao Yu

Keyword(s):

Listeria Monocytogenes ◽

Antimicrobial Resistance ◽

Antimicrobial Susceptibility ◽

Gel Electrophoresis ◽

Susceptibility Testing ◽

Pulsed Field Gel Electrophoresis ◽

Restriction Patterns ◽

Pulsed Field ◽

Retail Food

Listeria monocytogenes isolates recovered from retail foods were characterized by serotyping, genotyping using pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Six serotypes were identified among the isolates and the predominant serotype was 1/2a (42, 48.8%). PFGE differentiated the 86 isolates into 23 ApaI restriction patterns. Twenty-five (29.1%) of the L.monocytogenes strains exhibited resistance to at least one antimicrobial. Resistance to ciprofloxacin (17.4% of the isolates were resistant) and resistance to tetracycline (17.4%) were observed frequently.

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Characterization of antimicrobial resistance genes in Haemophilus parasuis isolated from pigs in China

10.7287/peerj.preprints.3386v1 ◽

2017 ◽

Author(s):

Yongda Zhao ◽

Lili Guo ◽

Jie Li ◽

Xianhui Huang ◽

Binghu Fang

Keyword(s):

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Gel Electrophoresis ◽

Antibiotic Resistance Genes ◽

Pulsed Field Gel Electrophoresis ◽

Haemophilus Parasuis ◽

Pulsed Field ◽

Antimicrobial Resistance Genes

Background: Haemophilus parasuis is a common porcine respiratory disease that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China. Methods: We screened 143 H. parasuis isolates for the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). The genetic relatedness among the strains was analyzed by pulsed-field gel electrophoresis. Results: We found 14 antimicrobial resistance genes were present in these isolates, including TEM-1, ROB-1.ermB,ermA ,flor, catl,tetB,tetC, rmtB, rmtD, aadA1, aac(3’)-ⅡC, sul1, and sul2 genes. Interestingly, one isolate carried 5 antibiotic resistance genes (tetB, tetC, flor, rmtB, sul1). The genes tetB, rmtB, and flor were the most prevalent resistance genes in H. parasuis in China. Alterations in the gyrA gene (S83F/Y, D87Y/N/H/G) were detected in 81% of the strains and parC mutations were often accompanied by a gyrA mutation. pulsed-field gel electrophoresis typing revealed 51 unique patterns in the isolates carrying antibiotic resistance genes indicating considerable genetic diversity and suggesting the genes were spread horizontally. Discussion: The current study demonstrated that the high antibiotic resistance of H. parasuis in piglets is a combination of transferable antibiotic resistance genes and multiple target gene mutations. GyrA gene mutation also was the most important role in quinolone resistance. These data provide novel insights for the better understanding of the prevalence and epidemiology of antimicrobial resistance in H. parasuis.

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Characterization of antimicrobial resistance genes in Haemophilus parasuis isolated from pigs in China

10.7287/peerj.preprints.3386 ◽

2017 ◽

Author(s):

Yongda Zhao ◽

Lili Guo ◽

Jie Li ◽

Xianhui Huang ◽

Binghu Fang

Keyword(s):

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Gel Electrophoresis ◽

Antibiotic Resistance Genes ◽

Pulsed Field Gel Electrophoresis ◽

Haemophilus Parasuis ◽

Pulsed Field ◽

Antimicrobial Resistance Genes

Background: Haemophilus parasuis is a common porcine respiratory disease that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China. Methods: We screened 143 H. parasuis isolates for the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). The genetic relatedness among the strains was analyzed by pulsed-field gel electrophoresis. Results: We found 14 antimicrobial resistance genes were present in these isolates, including TEM-1, ROB-1.ermB,ermA ,flor, catl,tetB,tetC, rmtB, rmtD, aadA1, aac(3’)-ⅡC, sul1, and sul2 genes. Interestingly, one isolate carried 5 antibiotic resistance genes (tetB, tetC, flor, rmtB, sul1). The genes tetB, rmtB, and flor were the most prevalent resistance genes in H. parasuis in China. Alterations in the gyrA gene (S83F/Y, D87Y/N/H/G) were detected in 81% of the strains and parC mutations were often accompanied by a gyrA mutation. pulsed-field gel electrophoresis typing revealed 51 unique patterns in the isolates carrying antibiotic resistance genes indicating considerable genetic diversity and suggesting the genes were spread horizontally. Discussion: The current study demonstrated that the high antibiotic resistance of H. parasuis in piglets is a combination of transferable antibiotic resistance genes and multiple target gene mutations. GyrA gene mutation also was the most important role in quinolone resistance. These data provide novel insights for the better understanding of the prevalence and epidemiology of antimicrobial resistance in H. parasuis.

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