In vitro antifungal effect of potassium sorbate and sodium benzoate on the growth of fungi causing sinusitis

An in vitro gas production technique was used to screen different organic acids (formic, propionic, lactic, citric, and fumaric acid), organic salts (calcium formate, potassium sorbate, and sodium benzoate), and inorganic phosphoric acid for their ability to modulate microbial fermentation in the digestive tract of piglets. For the incubation, 40 ml of culture medium (53% buffer, 45% frozen ileal digesta, and 2% fresh faeces) was dispensed in vessels containing 5 ml of buffer, 0.5 g of feed, and 20 ìl of liquid or 20 mg of solid acidifiers. Gas production was measured every 15 min during the 24 h incubation at 39°C, and a Gompertz bacterial growth model was applied to the gas production data. Formic acid was the only acid that reduced the maximum rate of gas production (ìm) compared to that in the control treatment (P < 0.05). The ìm was slower in vessels with formic acid than in those with calcium formate, citric acid, and potassium sorbate (P < 0.05) Calcium formate increased the ìm compared to the control treatment (P < 0.05). The maximum volume of gas produced and the lag time did not differ between different acidifiers (P > 0.05). When investigating formic-acid-based mixtures that contained 15% of potassium sorbate and/or sodium benzoate, the estimated parameters for the Gompertz growth model did not differ from those for treatments with plain formic acid (P > 0.05). However, concentrations of total volatile fatty acids, acetic acid, propionic acid, and n-butyric acid were reduced by all the mixtures (P < 0.05), but not by plain formic acid (P > 0.05). In conclusion, organic acids and salts were found to differ in their ability to modulate microbial fermentation in the digestive tract of piglets. Mixing formic acid with potassium sorbate or sodium benzoate changed fermentation patterns, and the possibility to use them to enhance the antimicrobial effect of formic acid should be investigated further in vivo.;

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Evaluation of different salts for the control of lettuce varnish spot: when phytotoxicity rules

Canadian Journal of Plant Science ◽

10.1139/cjps-2017-0237 ◽

2018 ◽

Vol 98 (3) ◽

pp. 753-761 ◽

Cited By ~ 4

Author(s):

Maxime Delisle-Houde ◽

Vicky Toussaint ◽

Hicham Affia ◽

Russell J. Tweddell

Keyword(s):

Sodium Bicarbonate ◽

Sodium Carbonate ◽

Sodium Benzoate ◽

Leaf Tissue ◽

In Vitro Assays ◽

Potassium Sorbate ◽

Sodium Metabisulfite ◽

Pseudomonas Cichorii ◽

Foliar Symptoms

Five generally recognised as safe (GRAS) salts with antimicrobial activity were investigated for their potential use as bactericides for the control of lettuce varnish spot [Pseudomonas cichorii (Swingle) Stapp]. The phytotoxicity of salts was first assessed using greenhouse and in vitro assays. Greenhouse assays revealed that salts showed different levels of phytotoxicity. Potassium sorbate, sodium benzoate, and sodium carbonate at higher concentrations caused a noticeable decrease of growth along with foliar phytotoxicity symptoms while sodium metabisulfite and sodium bicarbonate caused exclusively foliar symptoms. Based on the phytotoxic doses 5% determined in vitro, salts can be ranked in ascending order of phytotoxicity as follows: sodium bicarbonate, potassium sorbate, sodium carbonate, sodium benzoate, and sodium metabisulfite. When applied at concentrations causing mild to moderate foliar symptoms of phytotoxicity and no noticeable effect on growth, salts did not significantly affect (p ≤ 0.01) survival of P. cichorii on lettuce leaf tissue and did not significantly reduce (p ≤ 0.01) varnish spot severity. Although sodium metabisulfite was applied at concentrations higher than the minimum inhibitory concentration and minimum bactericidal concentration, it did not affect P. cichorii survival on leaf tissue.

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Effect of Quinic Acid on the Growth of Some Wild Yeasts and Molds

Journal of Food Protection ◽

10.4315/0362-028x-48.4.327 ◽

1985 ◽

Vol 48 (4) ◽

pp. 327-329 ◽

Cited By ~ 4

Author(s):

HEIKKI KALLIO ◽

SEIJA AHTONEN ◽

SEPPO S. SARIMO

Keyword(s):

Sodium Benzoate ◽

Quinic Acid ◽

Inhibitory Effect ◽

Potassium Sorbate ◽

Lag Phase ◽

Antifungal Effect ◽

Hansenula Anomala ◽

Wild Yeasts ◽

Yeasts And Molds ◽

Byssochlamys Fulva

The effect of quinic acid on growth of wild yeasts (Hansenula anomala, Saccharomyces cerevisiae and Schizosaccharomyces pombe) and molds (Aspergillus amstelodami, Botrytis cinerea and Byssochlamys fulva) was investigated. Quinic acid alone had no antifungal effect on the microbes tested. Generation time of the yeasts remained unaltered in the presence of up to 1% quinic acid, whereas growth of the molds was accelerated. No synergistic effect of quinic acid together with potassium sorbate or sodium benzoate was observed. Quinic acid was antagonistic to the antifungal effects of both potassium sorbate and sodium benzoate on molds. In co-use with sorbate and benzoate, quinic acid shortened the lag phase of the growth of molds. The inhibitory effect of 0.01 to 0.02% sodium benzoate was almost completely eliminated by adding 1% quinic acid.

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Effect of different salts on the development of Fusarium solani var. coeruleum, a causal agent of potato dry rot

Phytoprotection ◽

10.7202/000377ar ◽

2009 ◽

Vol 89 (1) ◽

pp. 1-6 ◽

Cited By ~ 16

Author(s):

Mélanie R. Mecteau ◽

Joseph Arul ◽

Russell J. Tweddell

Keyword(s):

Fusarium Solani ◽

Sodium Benzoate ◽

Mycelial Growth ◽

Trisodium Phosphate ◽

Aluminium Chloride ◽

Potassium Sorbate ◽

Sodium Metabisulfite ◽

Dry Rot ◽

Potato Dry Rot

The objectives of this study were (1) to evaluate the effect of different salts on the in vitro development of Fusarium solani var. coeruleum, and (2) to evaluate the efficacy of the salts for reducing dry rot severity caused by the pathogen in potato tubers. The study showed that several salts significantly inhibited the mycelial growth of F. solani var. coeruleum. Aluminium acetate, aluminium chloride, sodium benzoate, sodium metabisulfite, potassium sorbate and trisodium phosphate completely inhibited mycelial growth. Exposure of F. solani var. coeruleum conidia to aluminium acetate, potassium sorbate, sodium benzoate, sodium metabisulfite or trisodium phosphate at 0.2 M resulted in 100% mortality of the conidia after 1 h while aluminium chloride and aluminium lactate caused 100% mortality after an exposure of 24 h. In order to evaluate the effect of salts on potato dry rot development, F. solani var. coeruleum-inoculated tubers were treated with the different salts and disease severity was evaluated following an incubation period of 7 d. Among the test salts, only aluminium chloride caused a significant reduction in potato dry rot compared with the control. The study points out the possibility of using aluminium chloride to control potato dry rot.

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Fermentation, losses, and aerobic stability of sugarcane silages treated with chemical or bacterial additives

Scientia Agricola ◽

10.1590/s0103-90162008000600004 ◽

2008 ◽

Vol 65 (6) ◽

pp. 589-594 ◽

Cited By ~ 34

Author(s):

André de Faria Pedroso ◽

Luiz Gustavo Nussio ◽

Daniele Rebouças Santana Loures ◽

Solidete de Fátima Paziani ◽

José Leonardo Ribeiro ◽

...

Keyword(s):

Ethanol Production ◽

Sodium Benzoate ◽

Dry Matter ◽

Ethanol Concentration ◽

Saccharum Officinarum ◽

Potassium Sorbate ◽

Dry Matter Digestibility ◽

Silage Fermentation ◽

Aerobic Stability

Utilization of sugarcane Saccharum officinarum L. silage is increasing in Brazil but intensive ethanol production during fermentation reduces forage quality. This experiment aimed to evaluate the effects of additives on fermentation and aerobic stability of sugarcane silages produced in minisilos. Treatments were (fresh basis): untreated silage (control), urea (5.0 g kg-1), sodium benzoate (1.0 g kg-1), potassium sorbate (0.3 g kg-1), Lactobacillus plantarum (1 x 10(6) cfu g-1), and Lactobacillus buchneri (3.64 x 10(5) cfu g-1). At the 94th day after ensilage, ethanol concentration was lower in urea, benzoate, sorbate and L. buchneri supplemented silages and higher in L. plantarum inoculated silage, as compared to control. Urea and benzoate treated silages showed the highest and L. plantarum treated silage the lowest in vitro dry matter digestibility. Effluent production was higher in the urea treated silage. Inoculation with L. buchneri reduced 50% ethanol production as compared to control. Urea and L. buchneri reduced yeast count. Aerobic stability was enhanced by L. buchneri and benzoate. Sodium benzoate and L. buchneri were the most promising additives, improving both silage fermentation and aerobic stability; inoculants containing L. plantarum can be deleterious to fermentation and conservation of sugarcane silages.

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Synergy between Salvia officinalis L. and some preservatives

Open Life Sciences ◽

10.2478/s11535-010-0026-0 ◽

2010 ◽

Vol 5 (4) ◽

pp. 491-495

Author(s):

Dragana Stanojević ◽

Ljiljana Čomić ◽

Olgica Stefanović

Keyword(s):

Aqueous Extract ◽

Sodium Nitrite ◽

Sodium Benzoate ◽

Synergistic Action ◽

Salvia Officinalis ◽

Assay Method ◽

Potassium Sorbate ◽

Checkerboard Assay ◽

Salvia Officinalis L

AbstractThe aim of the present study is to investigate the antibacterial activity of Salvia officinalis L. aqueous extracts and its synergistic action with preservatives sodium nitrite, sodium benzoate and potassium sorbate in vitro against selected food spoiling bacteria. Synergy was assessed by the checkerboard assay method and quantitatively represented by the FIC index. Synergistic action was established for aqueous extract/ sodium benzoate, aqueous extract/ potassium sorbate, aqueous extract/ sodium nitrite combinations. Synergy was detected in relation to: Agrobacterium tumefaciens, Bacillus subtilis and Proteus sp. Synergy was established at plant extract and preservative concentrations corresponding up to 1/8 MIC values.

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In vitro synergistic antibacterial activity of Salvia officinalis L. and some preservatives

Archives of Biological Sciences ◽

10.2298/abs1001167s ◽

2010 ◽

Vol 62 (1) ◽

pp. 167-174 ◽

Cited By ~ 25

Author(s):

Dragana Stanojevic ◽

Ljiljana Comic ◽

Olgica Stefanovic ◽

Slavica Solujic-Sukdolak

Keyword(s):

Antibacterial Activity ◽

Aqueous Extract ◽

Sodium Nitrite ◽

Sodium Benzoate ◽

Synergistic Action ◽

Salvia Officinalis ◽

Assay Method ◽

Potassium Sorbate ◽

Salvia Officinalis L

The aim of this work was to investigate the antibacterial activity of aqueous extracts of the species Salvia officinalis L. and its synergistic action with the preservatives sodium nitrite, sodium benzoate and potassium sorbate in vitro against selected food spoiling bacteria. Synergism was assessed by the checkerboard assay method and quantitatively represented by the FIC index. Synergistic action was established for aqueous extract/sodium benzoate, aqueous extract/potassium sorbate, aqueous extract/sodium nitrite combinations. Synergism was detected in relation to: Agrobacterium tumefaciens, Bacillus subtilis and Proteus sp. Synergism was established at plant extract and preservative concentrations corresponding up to 1/8 MIC values. Detected autoplagiarism. Link to the Editorial Decision <a href="http://dx.doi.org/10.2298/ABS1004251U">10.2298/ABS1004251U</a>

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Quantitative Analysis of Food Additives in a Beverage using High Performance Liquid Chromatography and Diode Array Detection Coupled with Chemometrics

Journal of AOAC International ◽

10.1093/jaocint/qsz009 ◽

2020 ◽

Vol 103 (3) ◽

pp. 779-783

Author(s):

Özlem Aksu Dönmez ◽

Şule Dinç-Zor ◽

Bürge Aşçı ◽

Abdürrezzak E Bozdoğan

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Sodium Benzoate ◽

High Performance ◽

Food Additives ◽

Potassium Sorbate ◽

Diode Array ◽

Diode Array Detection ◽

Ponceau 4R ◽

Array Detection

Abstract Background In many countries, the levels of synthetic food additives causing harm to humans have been determined and their use has been controlled by legal regulations. Sensitive, accurate and low-cost analysis methods are required for food additive determination. Objective In this study, a fast high performance liquid chromatography-diode array detection (HPLC-DAD) analytical methodology for quantification of sodium benzoate, potassium sorbate, ponceau 4R, and carmoisine in a beverage was proposed. Methods Partial least squares (PLS) and principal component regression (PCR) multivariate calibration methods applied to chromatograms with overlapped peaks were used to establish a green and smart method with short isocratic elution. A series of synthetic solutions including different concentrations of analytes were used to test the prediction ability of the developed methods. Conclusions The average recoveries for all target analytes were in the range of 98.27–101.37% with average relative prediction errors of less than 3%. The proposed chemometrics-assisted HPLC-DAD methods were implemented to a beverage successfully. Analysis results from sodium benzoate, potassium sorbate, ponceau 4R, and carmoisine in a beverage by PLS-2 and PCR were statistically compared with conventional HPLC. Highlights The HPLC methods coupled with the PLS-2 and PCR algorithm could provide a simple, quick and accurate strategy for simultaneous determination of sodium benzoate, potassium sorbate, ponceau 4R, and carmoisine in a beverage sample.

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A preliminary study on antifungal effect of TiO2-based paints in natural indoor light

Polish Journal of Chemical Technology ◽

10.2478/v10026-010-0050-x ◽

2010 ◽

Vol 12 (4) ◽

pp. 53-57 ◽

Cited By ~ 9

Author(s):

Agata Markowska-Szczupak ◽

Krzysztof Ulfig ◽

Barbara Grzmil ◽

Antoni Morawski

Keyword(s):

Antifungal Activity ◽

Aspergillus Niger ◽

Trichoderma Viride ◽

Photocatalytic Reaction ◽

X Ray Diffraction ◽

Antifungal Effect ◽

X Ray ◽

Preliminary Study ◽

Crystalline Forms ◽

Growth Of Fungi

A preliminary study on antifungal effect of TiO2-based paints in natural indoor light The antifungal activity of four commercial photocatalytic paints (KEIM Ecosil ME, Titanium FA, Photo Silicate and Silicate D) in natural indoor light was investigated. The paints contained TiO2 in rutile and anatase crystalline forms as evidenced by means of the X-ray diffraction analysis. In most cases the paints inhibited growth of fungi viz. Trichoderma viride, Aspergillus niger, Coonemeria crustacea, Eurotium herbariorum, and Dactylomyces sp. The KEIM Ecosil ME paint displayed the highest antifungal effect in the light, which could be explained with the highest anatase content. The paint antifungal activity and the fungal sensitivity to the TiO2-mediated photocatalytic reaction both decreased in the following orders: KEIM Ecosil ME > Titanium FA > Photo Silicate > Silicate D and T. viride > Dactylomyces sp. > A. niger > E. herbariorum.

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Resistance of Yeast Flora of Labaneh to Potassium Sorbate and Sodium Benzoate

Journal of Dairy Science ◽

10.3168/jds.s0022-0302(97)76180-0 ◽

1997 ◽

Vol 80 (10) ◽

pp. 2304-2309 ◽

Cited By ~ 20

Author(s):

Ghadeer F. Mihyar ◽

Mohammed I. Yamani ◽

Ali K. Al-Sa’ed

Keyword(s):

Sodium Benzoate ◽

Potassium Sorbate ◽

Yeast Flora

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