scholarly journals In vitro antioxidant effects of Trigonella foenum graecum (L.) Fenugreek seed extract on sheep red blood cells

2012 ◽  
Vol 6 (38) ◽  
pp. 5119-5127 ◽  
Author(s):  
Dinakaran Vasudevan ◽  
Sridharan Subhashree ◽  
Periyasamy Latha ◽  
Sudha Rani Sankaramoorthy
1993 ◽  
Vol 265 (1) ◽  
pp. C99-C105 ◽  
Author(s):  
Z. C. Xu ◽  
P. B. Dunham ◽  
B. Dyer ◽  
R. Blostein

Na(+)-K+ pumps of red blood cells from sheep of the low-K+ (LK) phenotype undergo differentiation during circulation, manifested in part by a striking increase in sensitivity to inhibition by intracellular K+ (Ki). Pumps of red blood cells from sheep from the allelic phenotype, high K+ (HK), do not undergo this type of maturation. The hypothesis was tested that the Lp antigen, found on LK but not HK cells, is responsible for the maturation of LK pumps. Lp antigens have been shown to inhibit LK pumps because anti-Lp antibody stimulates the pumps by relieving inhibition by the antigen. Lp antigens were recently shown to be molecular entities separate from Na(+)-K+ pumps [Xu, Z.-C., P. Dunham, J. Munzer, J. Silvius, and R. Blostein. Am. J. Physiol. 263 (Cell Physiol. 32): C1007-C1014, 1992]. The test of the hypothesis was to modify the Lp antigens of immature LK red blood cells with two kinds of treatments, anti-Lp antibody and trypsinization (which cleaves Lp), and to observe the effects of these treatments on maturation of pumps during culture of the cells in vitro. Both of these treatments prevented the maturation of the kinetics of the pumps to the Ki-sensitive pattern, supporting the hypothesis that interaction of the pumps with Lp antigens is responsible for the maturation of the pumps. Strong supportive evidence came from experiments on Na(+)-K+ pumps from rat kidney delivered into immature LK sheep red blood cells by microsome fusion.(ABSTRACT TRUNCATED AT 250 WORDS)


2001 ◽  
Vol 67 (6) ◽  
pp. 2515-2525 ◽  
Author(s):  
Julien Brillard ◽  
Carlos Ribeiro ◽  
Noël Boemare ◽  
Michel Brehélin ◽  
Alain Givaudan

ABSTRACT Xenorhabdus spp. and Photorhabdus spp. are major insect bacterial pathogens symbiotically associated with nematodes. These bacteria are transported by their nematode hosts into the hemocoel of the insect prey, where they proliferate within hemolymph. In this work we report that wild strains belonging to different species of both genera are able to produce hemolysin activity on blood agar plates. Using a hemocyte monolayer bioassay, cytolytic activity against immunocompetent cells from the hemolymph ofSpodoptera littoralis (Lepidoptera: Noctuidae) was found only in supernatants of Xenorhabdus; none was detected in supernatants of various strains of Photorhabdus. During in vitro bacterial growth of Xenorhabdus nematophila F1, two successive bursts of cytolytic activity were detected. The first extracellular cytolytic activity occurred when bacterial cells reached the stationary phase. It also displayed a hemolytic activity on sheep red blood cells, and it was heat labile. Among insect hemocyte types, granulocytes were the preferred target. Lysis of hemocytes by necrosis was preceded by a dramatic vacuolization of the cells. In contrast the second burst of cytolytic activity occurred late during stationary phase and caused hemolysis of rabbit red blood cells, and insect plasmatocytes were the preferred target. This second activity is heat resistant and produced shrinkage and necrosis of hemocytes. Insertional inactivation of flhD gene in X. nematophilaleads to the loss of hemolysis activity on sheep red blood cells and an attenuated virulence phenotype in S. littoralis (A. Givaudan and A. Lanois, J. Bacteriol. 182:107–115, 2000). This mutant was unable to produce the early cytolytic activity, but it always displayed the late cytolytic effect, preferably active on plasmatocytes. Thus, X. nematophila produced two independent cytolytic activities against different insect cell targets known for their major role in cellular immunity.


Nature ◽  
1973 ◽  
Vol 243 (5407) ◽  
pp. 408-410 ◽  
Author(s):  
MICHAEL K. HOFFMANN ◽  
DELF SCHMIDT ◽  
HERBERT F. OETTGEN

2011 ◽  
Vol 61 (3) ◽  
pp. 303-317 ◽  
Author(s):  
Saber A. Sakr ◽  
Ezz M. El-Gamal

AbstractAdriamycin (ADR) is an anticancer drug used in treatment of a variety of neoplastic lesions. Its use is limited due to diverse toxicities including cardiotoxicity, hepatotoxicity and nephrotoxicity. The present work was conducted to study the effect of fenugreek seed extract on nephrotoxicity induced by ADR in albino rats. Administrating animals with a single dose of ADR (10 mg/kg body weight) induced histopathological, immunohistochemical and biochemical alterations. Kidneys of ADR-treated rats showed many histopathological alterations. The renal tubules were degenerated and the glomeruli were atrophied. The intertubular spaces were infiltrated by inflammatory leucocytic cells. An increase in expression of alfa-SMA was recorded. ADR caused marked elevation in serum creatinine and blood urea nitrogen. Moreover, ADR administration to animals significantly increased the concentration of malondialdehyde and decreased the activity of superoxide dismutase (SOD) in kidney tissues. These changes were time-dependent. Treating animals with ADR and aqueous seed extract of fenugreek (0.4 g/kg) led to an improvement in the histological structure of the kidney together with a decrease in expression of α-SMA. Urea and creatinine were significantly decreased. Moreover, fenugreek treatment reduced the concentration of malondialdehyde (lipid peroxidation marker) and increased the activity of antioxidant enzyme, SOD. In conclusion, the results of the present work indicated that fenugreek seeds had ameliorative effect on kidney damage induced by ADR and this may be mediated by its potent antioxidant effects


2019 ◽  
Vol 29 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Lucie Widowati ◽  
Ani Isnawati ◽  
Sukmayati Alegantina ◽  
Fifi Retiaty

Abstract The proportion of stunting in children under 5 years old in Indonesia was approximately 30,8% in 2018. One of the things that is presumably related to the occurrence of stunting is lack of nutrition that can be attributed to exclusive breastfeeding. Fenugreek seeds have properties as laktogoga and moringa leaves in addition to having efficacy as lactagoga, also have advantage in nutritional content, particularly high protein content, groups of minerals and vitamins. The aim of the study was to assess the potential of the formula fenugreek seed extract (Trigonella foenum-graecum L) and moringa leaves (Moringa oleifera Lamk.) as a supplement to release milk while at the same having nutrient content. Methods using the test weighting method in pregnant female rats by measuring the volume of milk taken by baby rats. Test were carried out on five groups of mother rats namely groups than were given : fenugreek and moringa extract (1: 1) with different doses I, II and III; comparison group (moloco) and control group (distilled water). Each mother fed five rats, provision of extract was carried out starting the first day after the mother gave birth, and measurement were take on 6th, 11th, 16th to 21st day The volume of milk consumed by five rats was counted as difference of body weight after and before breastfeeding. Moringa nutritional assessment is carried out using standard methods on, iron, calcium and potassium content; vitamin A and vitamin C content and protein level. Fenugreek-moringa extract (1: 1) dose of 30 mg/200g bb, can increase the volume of breast milk of female rat larger than the untreated group (p≤0.05). The cumulative weight gain of rat infants was greater than the control group (p≤0.05), according to the nutrients obtained from the fenugreek-moringa extract. Moringa leaves as a component of extract, have high nutritional value of minerals, vitamins and proteins and have advantages as lactogogum. Conclusion: The administration of moringa leaf and fenugreek seed extract (1: 1) dose of 30 mg / 200 g bb in breastfeeding female rats has a 2.4 times greater potential in increasing milk expenditure compared to breastfeeding female rats who did not get the same extract. Fenugreek-moringa extract has superior potential as laktagogum and at the same time a high nutritional supplement. Abstrak Proporsi kejadian stunting pada anak usia di bawah 5 tahun (balita) di Indonesia tercatat sekitar 30,8% pada tahun 2018. Salah satu hal yang diduga berkaitan dengan terjadinya stunting adalah gizi kurang yang dapat dikaitkan dengan pemberian air susu ibu (ASI) eksklusif. Biji klabet memiliki khasiat sebagai laktagoga dan daun kelor selain mempunyai khasiat sebagai laktagoga, juga mempunyai keunggulan pada kandungan gizinya, terutama kandungan protein yang tinggi, golongan mineral dan vitamin. Penelitian bertujuan untuk menilai potensi formula ekstrak biji klabet (Trigonella foenum-graecum L.) dan daun kelor (Moringa oleifera Lamk.) sebagai suplemen pelancar pengeluaran air susu sekaligus memiliki kandungan nutrisi yang tinggi. Metode menggunakan test weighting method pada tikus betina hamil, dengan cara mengukur volume air susu yang diminum oleh bayi tikus. Dilakukan uji pada lima kelompok induk tikus yaitu: kelompok yang diberi ekstrak klabet dan kelor (1:1) dengan dosis I, II dan III yang berbeda; kelompok pembanding (moloco) dan kelompok kontrol (akuades). Tiap induk tikus menyusui lima ekor anak tikus. Pemberian bahan uji dilakukan mulai hari pertama setelah induk tikus melahirkan, dan pengukuran dilakukan pada hari ke-6, 11, 16 sampai 21. Volume air susu yang diminum lima ekor anak tikus dihitung sebagai selisih berat badan sesudah dan sebelum disusui induknya. Penilaian gizi kelor dilakukan menggunakan metoda standar, terhadap kandungan mineral besi, kalsium dan kalium; kandungan vitamin A dan vitamin C, serta kadar protein. Hasil penelitian menunjukkan ekstrak klabet-kelor (1:1) dosis 30 mg/200g bb, dapat memperbanyak pengeluaran volume air susu induk tikus lebih besar dari kelompok yang tidak diberi perlakuan (p≤0,05). Pertambahan berat kumulatif bayi tikus, lebih besar dibandingkan kelompok kontrol (p≤0,05), sesuai dengan gizi yang didapat dari ekstrak klabet-kelor. Daun kelor sebagai komponen ekstrak, mempunyai nilai gizi mineral, vitamin, dan protein yang tinggi serta memiliki keunggulan sebagai laktogogum. Kesimpulan penelitian adalah pemberian ekstrak biji klabet-daun kelor (1:1) dosis 30 mg/200g bb pada tikus betina menyusui mempunyai potensi 2,4 kali lebih besar dalam meningkatkan pengeluaran air susu dibandingkan kelompok tikus betina menyusui yang tidak mendapatkan ekstrak yang sama. Ekstrak biji klabet-kelor mempunyai potensi unggul sebagai laktagogum dan sekaligus suplemen dengan gizi tinggi.


1991 ◽  
Vol 29 (3) ◽  
pp. 235-240 ◽  
Author(s):  
Makoto Nakamura ◽  
Tomoaki Yoshida ◽  
Ken-ichi Isobe ◽  
Takashi Iwamoto ◽  
S.M. Jamshedur Rahman ◽  
...  

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