Determination of antioxidant and anti-inflammatory activities, as well as in vitro cytotoxic activities of extracts of Anastatica hierochuntica (Kaff Maryam) against HeLa cell lines

In our continuing interest on SarawakGarciniaspecies, we carried out the evaluation of antioxidant, anti-inflammatory and cytotoxic activities on the methanolic extracts ofGarcinia nervosa. The extracts were prepared from its air-dried grounded leaves and barks. The evaluation of antioxidant activities was done using the (2,2-diphenyl-1-picrylhydrazyl) DPPH radical scavenging assay and the result showed high radical scavenging activities. Meanwhile, the anti-inflammatory evaluation was performed using the lipoxygenase assay, hyaluronidase assay, and xanthine oxidase assay which showed, both of these extracts exhibited high anti-inflammatory properties. The lipoxygenase assay showed a high inhibition of enzyme activity for the barks extracts and a moderate enzyme activity for the leaves extracts. However, there were low inhibitions for both extracts in the hyaluronidase assay and only the barks extracts exhibited moderate antigout properties in the xanthine oxidase assay. For the cytotoxic assay, the extracts exhibited positive responses against the three cancer cell lines, the HeLa cell lines, MCF-7 cell lines, and HT-29 cell lines. Thus,Garcinia nervosacontains high antioxidativeand anti-inflammation properties, which have great potential in the development of pharmaceutical and dermatological products.

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In-vitro Cytotoxicity and In-silico Insights of the Multi-target Anticancer Candidates from Haplophyllum tuberculatum

Borneo Journal of Pharmacy ◽

10.33084/bjop.v4i3.1955 ◽

2021 ◽

Vol 4 (3) ◽

pp. 192-201

Author(s):

Mosab Yahya Al-Nour ◽

Ahmed H Arbab ◽

Mohammad Khalid Parvez ◽

Arwa Y Mohamed ◽

Mohammed S Al-Dosari

Keyword(s):

Hela Cell ◽

Anticancer Activity ◽

Cell Lines ◽

In Vitro Cytotoxicity ◽

Binding Modes ◽

Web Servers ◽

Hela Cell Lines ◽

Ic50 Values ◽

Haplophyllum Tuberculatum

This study aimed to investigate the anticancer activity of Haplophyllum tuberculatum(Forsk.) aerial parts ethanol extract and fractions and reveal the potential anticancer targets, binding modes, pharmacokinetics, and toxicity properties of its phytoconstituents. MTT assay was used to investigate the anticancer activity. TargetNet, ChemProt version 2.0, and CLC-Pred web servers were used for virtual screening, and Cresset Flare software was used for molecular docking with the 26 predicted targets. Moreover, pkCSM, swiss ADME, and eMolTox web servers were used to predict pharmacokinetics and safety. Ethanolic extracts of H. tuberculatum on HepG2 and HeLa cell lines showed promising activities with IC50 values 54.12 and 48.1 µg/mL, respectively. Further, ethyl acetate fraction showed the highest cytotoxicity on HepG2 and HeLa cell lines with IC50 values 41.7 and 52.31 µg/mL. Of 70 compounds screened virtually, polygamain, justicidin A, justicidin B, haplotubine, kusunokinin, and flindersine were predicted as safe anticancer drugs candidates. They showed the highest binding scores with targets involved in cell growth, proliferation, survival, migration, tumor suppression, induction of apoptosis, metastasis, and drug resistance. Our findings revealed the potency of H. tuberculatum as a source of anticancer candidates that further studies should support.

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GC–MS analysis for compound identification in leaf extract of Lumnitzera racemosa and evaluation of its in vitro anticancer effect against MCF7 and HeLa cell lines

Journal of King Saud University - Science ◽

10.1016/j.jksus.2019.01.014 ◽

2020 ◽

Vol 32 (1) ◽

pp. 780-783 ◽

Cited By ~ 1

Author(s):

G. Eswaraiah ◽

K. Abraham Peele ◽

S. Krupanidhi ◽

M. Indira ◽

R. Bharath Kumar ◽

...

Keyword(s):

Hela Cell ◽

Cell Lines ◽

Leaf Extract ◽

Compound Identification ◽

Anticancer Effect ◽

Ms Analysis ◽

Hela Cell Lines ◽

Lumnitzera Racemosa

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Accumulation of betulinic, oleanolic, and ursolic acids in In vitro cell cultures of Lantana camara L. and their significant cytotoxic effects on HeLa cell lines

Biotechnology and Bioprocess Engineering ◽

10.1007/s12257-010-0054-7 ◽

2010 ◽

Vol 15 (6) ◽

pp. 1038-1046 ◽

Cited By ~ 30

Author(s):

Priyanka Srivastava ◽

Naresh Kasoju ◽

Utpal Bora ◽

Rakhi Chaturvedi

Keyword(s):

Hela Cell ◽

Cell Lines ◽

Cell Cultures ◽

Lantana Camara ◽

Cytotoxic Effects ◽

Hela Cell Lines ◽

In Vitro Cell Cultures

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Evaluation of photodynamic therapy and nuclear imaging potential of subphthalocyanine integrated TiO2 nanoparticles in mammary and cervical tumor cells

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424619500639 ◽

2019 ◽

Vol 23 (07n08) ◽

pp. 908-915 ◽

Cited By ~ 3

Author(s):

Fatma Yurt ◽

Kasim Ocakoglu ◽

Ozge Er ◽

Hale Melis Soylu ◽

Mine Ince ◽

...

Keyword(s):

Photodynamic Therapy ◽

Hela Cell ◽

Cell Line ◽

Cell Lines ◽

Nuclear Imaging ◽

Target Tissue ◽

Hela Cell Lines ◽

Wi38 Cell

This study, subphthalocyanines (SubPc) and SubPc integrated TiO2 nanoparticles (SubPc-TiO[Formula: see text] were synthesized as novel photosensitizers. Their PDT effects were evaluated. Furthermore, nuclear imaging potential of [Formula: see text]I-labelled SubPc/SubPc-TiO2 were examined in mouse mammary carcinoma (EMT6) and cervix adenocarcinoma (HeLa) cell lines. The uptake results show that SubPc labelled with [Formula: see text]I radionuclide ([Formula: see text]I-SubPc) in EMT6 and HeLa cell lines was found to be approximately the same as in the WI38 cell line. However, the uptake values of SubPc-TiO2 labelled with [Formula: see text]I ([Formula: see text]I-SubPc-TiO[Formula: see text] in EMT6 and HeLa cell lines were determined to be two times higher than in the WI38 cell line. In other words, the target/non-target tissue ratio was identified as two in the EMT6 and HeLa cell lines. [Formula: see text]I-SubPc-TiO2 is promising for imaging or treatment of breast and cervix tumors. In vitro photodynamic therapy studies have shown that SubPc and SubPc-TiO2 are suitable agents for PDT. In addition, SubPc-TiO2 has higher phototoxicity than SubPc. As a future study, in vivo experiments will be held and performed in tumor-bearing nude mice.

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