scholarly journals Antibody response, viral load, viral clearance and growth rate in Tanzanian free-range local chickens infected with lentogenic Newcastle disease virus

2021 ◽  
Vol 13 (2) ◽  
pp. 98-105
Author(s):  
Richard Mushi James ◽  
Honorati Chiwanga Gaspar ◽  
Lemburis Mollel Esther ◽  
Walugembe Muhammed ◽  
Arsen Max Robert ◽  
...  
Keyword(s):  
Growth Rate ◽  
Viral Load ◽  
Antibody Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Viral Clearance ◽  
Free Range ◽  
Local Chickens

scholarly journals Genome-wide association study of antibody response to Newcastle disease virus in chicken

BMC Genetics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 42 ◽  
Author(s):  
Chenglong Luo ◽  
Hao Qu ◽  
Jie Ma ◽  
Jie Wang ◽  
Chunyu Li ◽  
...  
Keyword(s):  
Antibody Response ◽  
Newcastle Disease Virus ◽  
Association Study ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Genome Wide

scholarly journals Newcastle Disease Virus Induced Pathologies Severely Affect the Exocrine and Endocrine Functions of Pancreas in Chickens

2020 ◽  
Author(s):  
Zaib Ur Rehman ◽  
Shanhui Ren ◽  
Salman Latif Butt ◽  
Muhammad Naveed Anwar ◽  
Yingjie Sun ◽  
...  
Keyword(s):  
Growth Rate ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Plasma Levels ◽  
Vaccine Development ◽  
Histopathological Examination ◽  
Plasma Concentrations ◽  
Economic Losses ◽  
Mortality And Morbidity

Abstract Background: Newcastle disease virus (NDV) causes a highly contagious and devastating disease in poultry, Newcastle disease (ND), which is particularly characterized by extensive pathologies in the digestive, respiratory and nervous systems. ND cause heavy economic losses to the world poultry industry by decreasing growth rate, decrease egg productions, mortality and morbidity. Although, significant advances have been made in the vaccine development, but outbreaks are reported in vaccinated birds leading decrease growth rate. Methods: In this study, we report the damage caused by the NDV infection in the pancreas of vaccinated as well specific pathogen free chickens. Results: The histopathological examination of the pancreas showed sever damage in the form of partial depletion of zymogen granules, acinar cell vacuolization, necrosis, and apoptosis, congestion in the large and small vessels, sloughing of epithelial cells of pancreatic duct, and mild perivascular edema. Increased plasma levels of corticosterone, somatostatin, were observed in NDV infected chicks at 3 and 5-day post infection (DPI). Slight decrease in the plasma concentrations of the insulin were noticed at 5 DPI. Significant changes were not observed in the plasma levels of glucagon. Furthermore, NDV infection has decreases the activity and mRNA expression of amylase, lipase, and trypsin from the pancreas. Conclusion: Taken together, our findings highlight that NDV induces extensive tissue damage in pancreas, decrease the activity and expression of pancreatic enzymes and increase plasma corticosterone and somatostatin.

scholarly journals Survey for Newcastle Disease Virus Antibodies in Local Chickens, Ducks and Pigeons in Makurdi, Nigeria

2020 ◽  
Vol 8 (3) ◽  
pp. 55
Author(s):  
Abah Helen Owoya ◽  
Ochola Peter Friday ◽  
Ishaya Victor
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Local Chickens

scholarly journals Expression of Recombinant Fusion Protein from Local Isolate of Newcastle Disease Virus and Antibody Response to Recombinant Fusion Protein in Broiler Chickens Post-Vaccination

2020 ◽  
Vol 45 (2) ◽  
pp. 78-90
Author(s):  
R.W. Astuti ◽  
N. Wijayanti ◽  
A. Haryanto
Keyword(s):  
Antibody Response ◽  
Fusion Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Fusion Protein ◽  
Blue Staining ◽  
F Protein ◽  
Local Isolate ◽  
And Control

This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody response in the broiler sera which were injected with pure recombinant F protein compared with treated broilers that were vaccinated with commercial inactive NDV vaccines and control broilers without vaccination. The results showed that the recombinant F protein of NDV was successfully expressed, purified and visualized by SDS-PAGE with Coomassie Brilliant Blue staining and Westernblotting methods as a specific recombinant F protein with a molecular weight of 28 kDa. The pure recombinant F protein then was injected into broilers to determine the antibody response in broiler serum. Indirect ELISA showed that the production of antibodies was high in F protein vaccinated groups in comparison with other treated and control groups. The recombinant F protein has potential to be developed as a recombinant vaccine candidate after truncating the 6x His-tag part to obtain higher antibody respond if compared with antibody production in broiler serum post vaccinated with some commercially available broiler vaccines.

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