scholarly journals Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

2016 ◽  
Vol 78 (2) ◽  
pp. 94-109
Author(s):  
Sahib Zada ◽  
Abbas Ali Naseem ◽  
Seong-Joo Lee ◽  
Muhammid Rafiq ◽  
Imran Khan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Mineralogical Analysis ◽  
Isolation And Characterization

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

scholarly journals Isolation and Characterization of Phenolic Compounds and Anthocyanins from Murta (Ugni molinae Turcz.) Fruits. Assessment of Antioxidant and Antibacterial Activity

Molecules ◽  
2015 ◽  
Vol 20 (4) ◽  
pp. 5698-5713 ◽  
Author(s):  
Maria Junqueira-Gonçalves ◽  
Lina Yáñez ◽  
Carolina Morales ◽  
Muriel Navarro ◽  
Rodrigo A. Contreras ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Isolation And Characterization ◽  
Ugni Molinae

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 3 (2) ◽  
pp. 28 ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

scholarly journals OPTIMASI SINTESIS DAN KARAKTERISASI SABUN CAIR DARI KEMIRI (Aleurites moluccana) DENGAN BANTUAN GELOMBANG ULTRASONIC

2021 ◽  
Vol 6 (1) ◽  
pp. 1-10
Author(s):  
Aman Santoso ◽  
◽  
Syafiuddin Syafiuddin ◽  
Dedek Sukarianingsih ◽  
Sumari Sumari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Fatty Acid Content ◽  
Chemical Properties ◽  
Ultrasonic Waves ◽  
Raw Material ◽  
Hazelnut Oil ◽  
Liquid Soap ◽  
Liquid Form ◽  
Isolation And Characterization

Liquid soap has become a basic necessity, especially during the Covid pandemic. The quality of soap is influenced by the type of raw material used. Candlenut oil saponification with potassium hydroxide produces liquid soap which has the potential to be anti-bacterial. Ultrasonic waves (US) produce cavitation which speeds up the reaction. This study aims: (1) Synthesis and characterization of liquid hazelnut oil soap, (2) to determine the antibacterial activity of liquid soap. The stages in this study were: (1) isolation and characterization of hazelnut oil, (2) making liquid soap at various alkaline concentrations with US (3) characterizing liquid soap (4) testing for antibacterial activity. The results showed that liquid soap can be made by saponification with KOH hazelnut oil, obtained physical properties of viscous liquid form, yellowish white color, distinctive aroma. The chemical properties of liquid soap have a pH of 8.46, a density of 1.10 g/mL, a free fatty acid content of 0.34%, a free alkaline content of 0.05%, a viscosity of 1,460 cSt and a surface tension of 4.33 x 10-4 N/m. Candlenut liquid soap has antibacterial activity in the weak category against E. coli bacteria and in the moderate category against S. aureus bacteria

scholarly journals Isolasi dan Karakterisasi Fungi Endofit Umbi Porang (Amorphophallus muelleri Blume) sebagai Antibakteri

BIOSCIENTIAE ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 55
Author(s):  
Muhammad Fazrul Rahman ◽  
Witiyasti Imaningsih ◽  
Sasi Gendro Sari
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
E Coli ◽  
Isolation And Characterization ◽  
Venomous Animal ◽  
Animal Bites ◽  
Aspergillus Sp ◽  
Escherichia Coli Bacteria

Medicinal plants such as porang (Amorphophallus muelleri Blume) can produce bioactive compounds from plants-associated endophytes. Therefore, medicinal plants were a sources of isolation of endophytic fungi and endophytic fungi were a sources of secondary metabolites that have anticancer, antimalarial, antimicrobial, and so on. A Porang tuber has been used as a medicine for boils, medicine for sliced wounds and medicine for wounds due to venomous animal bites. The research was aimed to carry out isolation and characterization of endophytic fungi from porang tuber, and to test the ability of endophytic fungi from porang tuber as antibacterial against gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) bacteria. Isolation of endophytic fungi from porang tubers succeeded in getting five different fungi isolates. Based on macroscopic and microscopic characteristics, endophytic fungi isolated from porang tuber were members of the genus Curvularia, Penicillium, 2 isolates of Aspergillus, and 1 isolate that had not been identified. Porang endophytic fungi had antibacterial activity against S. aureus, but did not have antibacterial activity against E. coli bacteria. Endophytic fungi that had antibacterial activity were Curvularia sp., Penicillium sp., Aspergillus sp.

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