A Novel Approach: Effect of polarity Index of mobile phase on Retention Time of Antihyperlipidemic Antihypertensive and Angiotensin inhibiting Drugs in RP-HPLC Method

A reverse phase HPLC method is developed for the determination of Raloxifene in pharmaceutical dosage forms. Chromatography was carried out on an inertsil C18 column using a mixture of acetonitrile and phosphate buffer (30:70 v/v) as the mobile phase at a flow rate of 1 mL/min. Detection was carried out at 290 nm .The retention time of the drug was 10.609 min. The method produced linear responses in the concentration range of 0.5-200 µg/mL of Raloxifene. The method was found to be applicable for determination of the drug in tablets.

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APPLICATION OF VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF ARMODAFINIL IN BULK AND FORMULATION

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2017v9i5.22162 ◽

2017 ◽

pp. 158-161

Author(s):

Devi Ramesh ◽

Mohammad Habibuddin

Keyword(s):

Retention Time ◽

Mobile Phase ◽

Hplc Method ◽

Reverse Phase Hplc ◽

Reverse Phase ◽

Rp Hplc ◽

Ich Guidelines ◽

Validation Parameters ◽

Isocratic Mode

Objective: The objective of the present study is to develop and validate a simple, rapid, sensitive reverse phase HPLC method for the determination of Armodafinil present in bulk and its pharmaceutical formulations.Methods: The chromatographic separation was achieved by using Hypersil ODS C-18 (150 x 4.6 mm, 5Âµ) in an isocratic mode with mobile phase methanol: phosphate buffer 3.0 (60:40 %v/v) was used. The flow rate was 1 ml/min and effluent was monitored at 225 nm. The method was validated for validation parameters i.e. linearity, accuracy, precision and robustness according to ICH guidelines.Results: The retention time of Armodafinil was 4.2 min and the linearity range of the method was 500-20000ng/ml with regression (r2) coefficient 0.9998. The method was validated for precision, accuracy, robustness and which were found to be within the acceptable limits according to the ICH guidelines. Also, the method was successfully applied for the estimation of Armodafinil in the marketed formulation of Nuvigil and the recovery was found to be>98%.Conclusion: The developed method possess good selectivity, specificity, there is no interference found in the blank at a retention time of ARM and good correlation between the peak area and concentration of the drugs under prescribed conditions. Hence, the method can be applied for routine analysis of Armodafinil.Â

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Development and Validation of RP-HPLC for Estimation of Neratinib in Bulk and Tablet Dosage Form

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2019.110202 ◽

2019 ◽

Vol 11 (02) ◽

Author(s):

M Lakshmi Kanth ◽

B Raj Kama

Keyword(s):

Flow Rate ◽

Retention Time ◽

Chromatographic Separation ◽

Mobile Phase ◽

Dosage Form ◽

Hplc Method ◽

Rp Hplc ◽

Monopotassium Phosphate ◽

Development And Validation ◽

Tablet Dosage

An accurate RP-HPLC method developed for the estimation of Neratinib in bulk and tablet dosage form. The method is and validated for parameters linearity, accuracy, suitability, specificity, precession, LOD, LOQ and robustness. An Altima column (150 mm × 4.6 mm × 5μ) used for chromatographic separation within a runtime of 6 min. The mobile phase buffer (monopotassium phosphate) and acetonitrile (60:40 v/v) with 0.1% formic acid is used. The flow rate maintained at 1.0 ml/min with the effluents monitored at 215 nm. The Neratinib analyzed at retention time of 4.001. The concentration linear over 30-180μg/ml with regression equation y = 6065.6x + 795.43 and regression co-efficient 0.999.

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Reverse-phase High-Performance Liquid Chromatography Method Development and Validation for Estimation of Glimepiride in Bulk and Tablet Dosage Form

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/10.25258/ijpqa.11.2.18 ◽

2020 ◽

Vol 11 (02) ◽

pp. 296-302

Author(s):

Aseem Kumar ◽

Anil Kumar Sharma ◽

Rohit Dutt

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Flow Rate ◽

Retention Time ◽

Mobile Phase ◽

High Performance ◽

Hplc Method ◽

Reverse Phase ◽

Rp Hplc ◽

Development And Validation

The present work demonstrates a simple, rapid, precise, specific, and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method for analyzing glimepiride in pure and tablet forms. The present method was developed using a C18 column 150 × 4.6 mm, with 5 μm, and packing L1 maintained at a temperature of 30°C. The mobile phase was prepared by dissolving 0.5 gram of monobasic sodium phosphate in 500 mL of distilled water, pH of the solution adjusted to 2.1 to 2.7 with 10% phosphoric acid, and added 500 mL of acetonitrile. The mobile phase was pumped in the highperformance liquid chromatography (HPLC) system at a flow rate of 1 mL/min, and separation was carried out at 228 nm, using an ultraviolet (UV) detector. The chromatographic separation was achieved with peak retention time (RT) at about 9.30 minutes, and the method was found to be linear over a concentration range of 40 to 140 μg/mL. The specificity of the method represented no interference of the excipients during the analysis, and stability testing after 24 hours also showed that the method is suitable and specific. The accuracy was between 99.93 to 99.96%, with limit of detection (LOD) and limit of quantitation (LOQ) being 0.354 μg/mL, 1.18 μg/mL, respectively. Satisfactory results were found for precision and robustness parameters during the development and validation stage for the analytical method. The proposed method was also adopted for the analysis of glimepiride tablets to improve the overall quality control. Using this method, symmetric peak shape was obtained with reasonable retention time. The retention time of glimepiride for six repetitions is 9.3 ± 0.1 minutes; the run time is 21 minutes. The proposed RP-HPLC method is a modification of the United States Pharmacopeia (USP) method, and it was found to be valid for glimepiride within concentration ranges 40 to 140 μg/mL, using C18 analytical columns, and isocratic elution with UV detection, and at 1 mL/min of flow rate.

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Method Development and Validation of Degradation Studies of Lenalidomide by RP-HPLC

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00744 ◽

2021 ◽

pp. 4281-4286

Author(s):

Punna Venkateshwarlu ◽

Mehul M. Patel

Keyword(s):

Retention Time ◽

Mobile Phase ◽

Method Development ◽

Hplc Method ◽

Mobile Phase Flow Rate ◽

Pharmaceutical Dosage Forms ◽

Rp Hplc ◽

System Suitability ◽

Wide Range

A simple, accurate, RP HPLC method was developed by this study determination of lenalidomide. This method is developed by Shimadzu LC -2010 HT by using C18 (250 X 4.6 X mm X 5µ) column in solvents Phosphate buffer: Acetonitrile (55:45) v/v as mobile phase and the temperature was maintained at 25°C. The mobile phase flow rate 1ml/min was pumped and sample wavelength was detected at 242nm by ultraviolet -visible spectrophotometer. The retention time was found 2.5 min. The number of theoretical plates and tailing factor for lenalidomide was observed 16199.817 (NLT 2000) and 1.128 (NMT 2). The method was validated for analytical standards such as linearity, accuracy, precision, system suitability and robustness. LOD and LOQ values obtained from regression of lenalidomide 0.058 and 0.174µg/ml. The regression equation of validated method for lenalidomide is Y=5223x+183075. In wide range of 25 to 150 (µg/ml) the linearity was observed. The method was validated and a recovery study indicates accuracy of this method. The Retention time less compared to established methods. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Lenalidomide in bulk drug and in its pharmaceutical dosage forms.

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RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF METFORMIN HCL AND CANAGLIFLOZIN IN PHARMACEUTICAL FORMULATION

INDIAN DRUGS ◽

10.53879/id.58.06.10607 ◽

2021 ◽

Vol 58 (06) ◽

pp. 49-59

Author(s):

Hemant Kumar T ◽

◽

Gowri Sankar D ◽

Keyword(s):

Flow Rate ◽

Retention Time ◽

Mobile Phase ◽

Orthophosphoric Acid ◽

Hplc Method ◽

Pharmaceutical Formulation ◽

Simultaneous Estimation ◽

Rp Hplc ◽

Interday Precision ◽

Metformin Hcl

A simple, specific, accurate, precise and stability-indicating RP-HPLC method was developed and validated for the simultaneous estimation of metformin HCl and canagliflozin in pharmaceutical formulation. The method was developed using the Altima C8 column (150 ×4.6 mm, 0.5 μm) with a mobile phase consisting of acetonitrile and 0.1 % orthophosphoric acid in water (62:38 %V/V) with a flow rate of 1 mL/min. Detection was carried out at 254 nm using a PDA detector. The retention time for metformin HCl and canagliflozin was found to be 2.282 and 3.339 min, respectively. The proposed method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was observed over a concentration range 15-225 μg/mL for metformin HCl (r2 =0.9995) and 5-40 μg/mL for canagliflozin (r2 =0.9988). The % RSD for intraday and interday precision was found to be 0.13 and 0.20 for metformin HCl and 0.18 and 0.20 for canagliflozin. The LOD and LOQ were found to be 0.16 μg/mL and 0.54 μg/mL for metformin HCl and LOD and LOQ were found to be 0.05 and 0.21 μg/mL for canagliflozin. Metformin HCl and canagliflozin were subjected to stress conditions of degradation including acidic, alkaline, oxidative, thermal and photolysis.

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A Validated RP – HPLC Method for Simultaneous Estimation of Cefixime and Cloxacillin in Tablets

E-Journal of Chemistry ◽

10.1155/2008/152390 ◽

2008 ◽

Vol 5 (3) ◽

pp. 648-651 ◽

Cited By ~ 3

Author(s):

G. Rathinavel ◽

P. B. Mukherjee ◽

J. Valarmathy ◽

L. Samueljoshua ◽

M. Ganesh ◽

...

Keyword(s):

Flow Rate ◽

Retention Time ◽

Phosphate Buffer ◽

Mobile Phase ◽

Hplc Method ◽

Simultaneous Estimation ◽

Rp Hplc

This paper presents a RP-HPLC method for the simultaneous estimation of cefixime and cloxacillin in tablets. The process was carried out on C18column (5 μm, 25 cm × 4.6 mm, i.d) using phosphate buffer (pH 5.0), acetonitrile and methanol in the ratio 80:17:3 respectively as a mobile phase at a flow rate of 2mL/min. Wavelength was fixed at 225 nm. The retention time of cefixime and cloxacillin was found to be 5.657 and 6.200 min, respectively. The developed method is rapid and sensitive and it can be used for estimation of combination of these drugs in tablets.

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Simultaneous estimation of gabapentin and methylcobalamin in bulk and pharmaceutical dosage form by RP-HPLC

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i1.2204 ◽

2019 ◽

Vol 9 (1) ◽

pp. 170-174

Author(s):

Anjali Bakshi ◽

K Monika ◽

Shweta Bhutada ◽

M. Bhagvan Raju

Keyword(s):

Particle Size ◽

Flow Rate ◽

Retention Time ◽

Mobile Phase ◽

Dosage Form ◽

Hplc Method ◽

Simultaneous Estimation ◽

Pharmaceutical Dosage Form ◽

Rp Hplc ◽

Ich Guidelines

A simple, selective, linear, precise, and accurate RP-HPLC method was developed and validated for the simultaneous estimation of Gabapentin & Methylcobalamin from bulk and formulation. Chromatographic separation was achieved Isocratically on an Inertsil C18 column (150x4.6, 5µ particle size) using a mobile phase Buffer: Acetonitrile in the ratio of 60:40 v/v. The flow rate was 1.0 ml/min, effluents were detected at 264 nm and 10µl of sample was injected. Retention time of Gabapentin & Methylcobalamin was found to be 2.7 and 4.13 min respectively. Linearity of the method was in the concentration range of 25-150 µg for Gabapentin & 0.125-0.750 µg for Methylcobalamin. Percent recoveries obtained for both the drugs were 100.00%. The percentage RSD for precision of the method was found to be less than 2%. The method was validated according to the ICH guidelines. The method developed was successfully applied for the analysis of simultaneous estimation of Gabapentin & Methylcobalamin tablets and was fairly good in comparison with other methods. Keywords: Gabapentin, Methylcobalamin, HPLC.

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A VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PANTOPRAZOLE AND CINITAPRIDE IN A PHARMACEUTICAL FORMULATION

INDIAN DRUGS ◽

10.53879/id.51.01.p0027 ◽

2014 ◽

Vol 51 (01) ◽

pp. 27-33

Author(s):

N. R Dighade ◽

◽

S. P. Padmane ◽

A. V. Kasture

Keyword(s):

Flow Rate ◽

Retention Time ◽

Mobile Phase ◽

Hplc Method ◽

Simultaneous Estimation ◽

Capsule Formulation ◽

Reverse Phase Hplc ◽

Reverse Phase ◽

Rp Hplc ◽

Effluent Flow Rate

The study describes a validated stability indicating reverse- phase HPLC method for the simultaneous estimation of pantoprazole and cinitapride in capsule formulation. The proposed RP-HPLC method utilizes an Eclipse XDB C18 Column (150 × 4.6 mm i.d., 5μm), optimum mobile phase consisting of 10 mM phosphate buffer: acetonitrile: THF in the ratio of 64:36:0.5 V/V (pH 3.5) V/V, effluent flow rate 1 mL/min and UV detection wavelength of 266 nm. The selected chromatographic conditions were found to effectively separate pantoprazole and cinitapride with retention time of 7.17 min and 3.56 min, respectively. Linearity for pantoprazole and cinitapride was found in the range of 2-30 μg/mL and 0.15- 2.28 μg/mL, respectively. The developed method was statistically validated for the linearity, accuracy, precision, robustness, ruggedness and specificity. The proposed method was found to be simple, accurate, precise, economical and specific. Therefore, it can be used for simultaneous analysis of these drugs in capsule formulation.

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A comparative technique using as Joint studying to prove structure and determination the Quantitative estimation of organic compound (Irbesartan) as drug in multicomponent tablet form

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v9i3.6 ◽

2019 ◽

Vol 9 (o3) ◽

Author(s):

Imad Tarek Hanoon ◽

Abed Mohammed Daheir AL-Joubory 2 ◽

Marwa Mohamed Saied 3

Keyword(s):

Mobile Phase ◽

Chemical Structure ◽

Quantitative Estimation ◽

Potassium Phosphate ◽

Hplc Method ◽

Pharmaceutical Dosage Forms ◽

Tablet Form ◽

Rp Hplc ◽

Percent Recovery

A simple , specific, accurate and precise RP-HPLC method was developed for determination of Irbesartan (IRB) in pharmaceutical dosage forms in tablets products and sachet using symmetry (L 1 ) column at 30°C . The signal was detected at 225 nm. A mobile phase dissolve 0.5 g of buffer potassium phosphate in 100 ml distilled water and adjust pH 2.7 , methanol and acetonitrile at ratio (40 :30 :30 ) . and flow rate 1.2ml/min -1 at pH=7.2 a mobile phase The percent recovery was detected 101 % and the linearity of concentration was 10-50 µg.ml -1 and supported this method by using (FT.I.R.) spectrum method for organic spectrophotometer to prove the chemical structure of this drug and some physical properties . we are obtained the result is identical of other literature . The proposed method was applied successfully for determination of the IRB in tablets products.

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