Phytochemical screening and anthelmintic activity of Artocarpus altilis extract

Background: Despite advances in hygiene and preventive medicine, parasitic diseases, particularly digestive parasitosis, remain a public health problem in tropical countries. Elaborate the ethnobotanical survey carried out in Brazzaville - Congo, 21 plants (divided into 20 families, 20 genera) were identified, among them 9 were selected for this study. These were: Ageratum conyzoides (L.) L., Rauvolfia mannii Stapf, Aloe buettneri A. Berger, Garcinia kola Heckel, Piper guineense Schumach & Thonn., Aframomum alboviolaceum (Ridl.) K. Schum., Plagiostyles africana (Müll .Arg.) Prain, Morinda lucida Benth, Cogniauxia podoleana Baill. Materials and Methods: The in vitro evaluation of the anthelmintic activity of the aqueous extracts of these 9 plants was determined at concentrations of 10, 25 and 50 mg/mL against Lumbricus terrestris used as animal support in comparison to the Mebendazole (20 mg/mL). The phytochemical screening realized by TLC to detect phytochemical families in plant, also, the antioxidant potential with DPPH radical was determined by UV-Visible spectrophometry in comparison to the Vitamin C. Results: Thus, P. guineense, A. conyzoides and A. alboviolaceum are those which presented significant vermicidal effect, after Mebendazole. Phytochemical screening into TLC revealed the co-presence of alkaloids, coumarins, flavonoids, tannins, sterols and terpenes.The antioxidant potential of the aqueous extracts of these different plants in comparison to vitamin C, has shown that the species A. Buettineri and A. alboviolaceum presented the best potential compared to 7 other species studied, with the concentration needed to reduce 50% of the DPPH radical (CR50) respectively 0.096 mg/mL for A. Buettineri, 0.098 mg/mL for A. alboviolaceum Berger and 0.015 mg/mL for vitamin C. Conclusion: There is modern drugs against intestinal worms, it is necessary to identify the plants with anthelmintic activity, to determine phytochemical compounds that would be responsible for their use by traditional healers to treat the intestinal parasitosis.

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In vitro thrombolytic, anthelmintic, anti-oxidant and cytotoxic activity with phytochemical screening of methanolic extract of Xanthium indicum leaves

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v10i4.23829 ◽

2015 ◽

Vol 10 (4) ◽

pp. 854 ◽

Cited By ~ 2

Author(s):

Antara Ghosh ◽

Sujan Banik ◽

Md. Ariful Islam

Keyword(s):

Cytotoxic Activity ◽

Methanolic Extract ◽

Radical Scavenging ◽

Anthelmintic Activity ◽

Positive Control ◽

Clot Lysis ◽

Total Phenolic ◽

Phytochemical Screening ◽

Anti Oxidant

Xanthium indicum is an important medicinal plant traditionally used in Bangladesh as a folkloric treatment. The current study was undertaken to evaluate thrombolytic, anthelmintic, anti-oxidant, cytotoxic properties with phytochemical screening of methanolic extract of X. indicum leaves. The analysis of phytochemical screening confirmed the existence of phytosetrols and diterpenes. In thrombolytic assay, a significant clot lysis was observed at four concentrations of plant extract compare to the positive control streptokinase (30,000 IU, 15,000 IU) and negative control normal saline. The extract revealed potent anthelmintic activity at different concentrations. In anti-oxidant activity evaluation by two potential experiments namely total phenolic content determination and free radical scavenging assay by 2, 2-diphenylpicrylhydrazyl (DPPH), the leaves extract possess good anti-oxidant property. In the brine shrimp lethality bioassay, the crude extract showed potent (LC50 1.3 μg/mL) cytotoxic activity compare to the vincristine sulfate as a positive control (LC50 0.8 μg/mL).

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Investigation of in-vitro anthelmintic activity of ethanolic leaf extract of Boerhavia diffusa (Nyctaginaceae) including pharmacognostical and phytochemical screening

Journal of Pharmacy Research ◽

10.1016/j.jopr.2013.08.009 ◽

2013 ◽

Vol 7 (8) ◽

pp. 774-780 ◽

Cited By ~ 1

Author(s):

Ramasubramania Raja Rajagopal

Keyword(s):

Leaf Extract ◽

Anthelmintic Activity ◽

Phytochemical Screening ◽

Ethanolic Leaf Extract ◽

Boerhavia Diffusa

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Evaluation of Anthelmintic Activity (In -vitro) of Boerhaavia diffusa Linn (Punarnava) Root Extract Including Phytochemical Screening

International Journal of Livestock Research ◽

10.5455/ijlr.20170330053039 ◽

2017 ◽

pp. 1

Author(s):

Sushma Shelu ◽

Chandrakant Jangde ◽

Rahul Nanotkar ◽

Rajesh Limsay ◽

Vijeya Awasthy ◽

...

Keyword(s):

Anthelmintic Activity ◽

Root Extract ◽

Phytochemical Screening ◽

Boerhaavia Diffusa

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Qualitative Phytochemical Screening and In-vitro Anthelmintic Activity of Adhatoda vasica (Acanthaceae)

Research Journal of Pharmacy and Technology ◽

10.5958/0974-360x.2017.00083.x ◽

2017 ◽

Vol 10 (2) ◽

pp. 414

Author(s):

Shabi R. Ruskin ◽

S. Ajina

Keyword(s):

Anthelmintic Activity ◽

Phytochemical Screening ◽

Adhatoda Vasica

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Anthelmintic Activities of Hexacosa-9, 11-dienoic Acid and 3-hydroxyurs-12-en-28-oic Acid Isolated from Spermacoce verticillata

Chemical Science International Journal ◽

10.9734/csji/2019/v28i130131 ◽

2019 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

S. Garba ◽

M. M. Adeyemi ◽

L. K. Musa

Keyword(s):

Fatty Acid ◽

Anthelmintic Activity ◽

Dienoic Acid ◽

Phytochemical Screening ◽

Active Components ◽

Standard Drug ◽

Pentacyclic Triterpenoid ◽

Death Time

The phytochemical screening, anthelmintics, and characterization of Spermacoce verticillata crude ethanolic extracts were carried out using standard methods. The research is aimed at investigating the in-vitro anthelmintic activity of Spermacoce verticillata and Cochlospermum tinctorium plants for possible active components. The results of phytochemical screening showed the presence of Alkaloids, Flavonoids, Tannin, Phenol, Fatty acid and Cardiac glycoside, Terpenoid and Saponin. The result of the anthelmintic activities showed that Spermacoce verticillata have paralysis time ranging from 25.9 -71.6 mins with death times ranging from 39 - 90.1 mins at concentrations range between 25 - 100 mg/cm3 The results of Chromatographic and Spectroscopic Analyses using TLC, CCH, IR, NMR GC-MS of c leads to the Isolation of two compounds namely Hexacosa-9,11-dienoic acid (fatty acid) and 3-hydroxyurs-12-en-28-oic acid (Pentacyclic triterpenoid) with paralysis/death time at concentration of 50 mg/cm3 of 22.5 mins and 36.3 mins for the fatty acid and 13.2 mins and 20.1 mins for the pentacyclic triterpenoid. The results were compared with standard drug (Albendazole) which showed higher activity than the crude extracts but lower than the isolated compounds. The results of this work therefore confirmed the traditional claim of using the plant for the treatment of helminthiasis disease.

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