Effect of Alcoholic Extract of Opuntia Ficus Indica on Semen Quality of Awassi Rams after Different Cooling Periods

2019 ◽  
Vol 10 (9) ◽  
pp. 924
Author(s):  
Abd AL-Kareem Abd AL-Reda Hobi ◽  
Ahmed Mahdi Abbas AL-Helal
Keyword(s):  
Semen Quality ◽  
Alcoholic Extract ◽  
Opuntia Ficus Indica

Protective effect of natural antioxidant Opuntia ficus-indica on ram semen quality stored at 5 °C for 72

2021 ◽  
Author(s):  
Larbi ALLAI ◽  
Xavier Druart ◽  
Pinar Terzioğlue ◽  
Noureddine Louanjli ◽  
Boubker Nasser ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Skim Milk ◽  
Butylated Hydroxytoluene ◽  
Emission Spectrometry ◽  
Control Group ◽  
Opuntia Ficus Indica ◽  
Analytical Instruments

Abstract Background Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5°C. The antioxidant activity of ETHEX was studied using DPPH• assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES), and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaâd rams with an artificial vagina. The ejaculates with more than 70 % motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1–8 % of ETHEX (37°C; 0.8 × 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48 and 72 h. Results The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2 % ETHEX in SM increased the sperm motility, viability, membrane integrity and decreased the abnormality, spontaneous and catalyzed lipids peroxidation (P < 0.05) up to 72 h. In addition, semen diluted with 1 and 2 % ETHEX decreased the level of DNA fragmentation compared to the control group (P < 0.05). Conclusions In conclusion the ETHEX could be recommended to improve the quality of liquid ram spermatozoa. However, its effects on sperm physiology and artificial insemination should be further studied.

Quality of ram spermatozoa separated with modified swim up method

2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Fluid Medium ◽  
Normal Morphology ◽  
The Mean ◽  
Mass Activity ◽  
Fresh Semen ◽  
Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

Effect of Fusarium toxin contaminated wheat on health, nutrient digestibility and semen quality of adult cockerels

2014 ◽  
Author(s):  
M. Ebrahem ◽  
Susanne Kersten ◽  
Christine Ehling ◽  
Hana Valenta ◽  
Ulrike Taylor ◽  
...  
Keyword(s):  
Semen Quality ◽  
Nutrient Digestibility ◽  
Fusarium Toxin

scholarly journals Hematologic Profile and Semen Quality of Male Timor Deer (Rusa timorensis) at Various Hierarchies

2018 ◽  
Vol 119 ◽  
pp. 012027
Author(s):  
D. Samsudewa ◽  
S. S. Capitan ◽  
C. C. Sevilla ◽  
R.S.A. Vega ◽  
P. P. Ocampo
Keyword(s):  
Semen Quality ◽  
Hematologic Profile

scholarly journals Are proton-pump inhibitors harmful for the semen quality of men in couples who are planning pregnancy?

2016 ◽  
Vol 106 (7) ◽  
pp. 1666-1672.e2 ◽  
Author(s):  
Nicole A. Huijgen ◽  
Maria A.J. de Ridder ◽  
Katia M. Verhamme ◽  
Gert R. Dohle ◽  
Ann M. Vanrolleghem ◽  
...  
Keyword(s):  
Proton Pump Inhibitors ◽  
Proton Pump ◽  
Semen Quality

scholarly journals Semen quality of 1559 young men from four cities in Japan: a cross-sectional population-based study

BMJ Open ◽  
2013 ◽  
Vol 3 (4) ◽  
pp. e002222 ◽  
Author(s):  
Teruaki Iwamoto ◽  
Shiari Nozawa ◽  
Makiko Naka Mieno ◽  
Katsunori Yamakawa ◽  
Katsuyuki Baba ◽  
...  
Keyword(s):  
Semen Quality ◽  
Young Men ◽  
Population Based ◽  
Cross Sectional ◽  
Population Based Study

scholarly journals Once-a-day Tadalafil administration improves the spermogram parameters in fertile patients

2015 ◽  
Vol 87 (3) ◽  
pp. 210 ◽  
Author(s):  
Antonio Corvasce ◽  
Giuseppe Albino ◽  
Teresa Leonetti ◽  
Agnese Filomena Buonomo ◽  
Ettore Cirillo Marucco
Keyword(s):  
Seminal Fluid ◽  
Semen Quality ◽  
Type A ◽  
Daily Treatment ◽  
Sperm Cells ◽  
Average Increase ◽  
Once Daily ◽  
Positive Effects ◽  
Semen Volume

Objectives: We explored the safety of Tadalafil once-daily treatment for 12 week and its effects on semen quality in a clinical subpopulation of men with psychogenic erectile dysfunction (ED). Patients and Methods: Twenty-seven men, between 19 and 35 years, unaware of their fertility status, suffering from psychogenic ED were enrolled. The examination of the seminal fluid was performed twice before administration of Tadalafil and twice after three months of continuous daily administration of Tadalafil 5 mg. The volume of the seminal fluid, the concentration of sperm, the concentration of sperm with type “a+b” motility, the concentration of sperm with type “a” motility, the concentration of normal sperm were taken into consideration. The results before treatment with Tadalafil (T0) and after 3 months of treatment (T3m) were compared. Results: The administration of once-daily Tadalafil 5mg, brings to an average increase of the total number of sperm cells, both total and fast motility (type a) and the percentage of nemasperms, and to an average increase of semen volume of only 0.41 ml. These quantitative and qualitative improvements of the seminal fluid resulted statistically significant as regard motility, nemasperm percentage and seminal fluid volume. No unespected safety findings were observed. Conclusions: Tadalafil administration improves the quality of sperm cells and seminal fluid: in particular motility, percentage of nemasperms and volume of seminal fluid. We emphasize the safety of the once-daily treatment with tadalafil 5mg and the positive effects on spermatogenesis.

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