Protective effect of natural antioxidant Opuntia ficus-indica on ram semen quality stored at 5 °C for 72

2021 ◽  
Author(s):  
Larbi ALLAI ◽  
Xavier Druart ◽  
Pinar Terzioğlue ◽  
Noureddine Louanjli ◽  
Boubker Nasser ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Skim Milk ◽  
Butylated Hydroxytoluene ◽  
Emission Spectrometry ◽  
Control Group ◽  
Opuntia Ficus Indica ◽  
Analytical Instruments

Abstract Background Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5°C. The antioxidant activity of ETHEX was studied using DPPH• assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES), and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaâd rams with an artificial vagina. The ejaculates with more than 70 % motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1–8 % of ETHEX (37°C; 0.8 × 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48 and 72 h. Results The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2 % ETHEX in SM increased the sperm motility, viability, membrane integrity and decreased the abnormality, spontaneous and catalyzed lipids peroxidation (P < 0.05) up to 72 h. In addition, semen diluted with 1 and 2 % ETHEX decreased the level of DNA fragmentation compared to the control group (P < 0.05). Conclusions In conclusion the ETHEX could be recommended to improve the quality of liquid ram spermatozoa. However, its effects on sperm physiology and artificial insemination should be further studied.

scholarly journals A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Suherni Susilowati ◽  
Imam Mustofa ◽  
Wurlina Wurlina ◽  
Indah Norma Triana ◽  
Suzanita Utama ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Artificial Insemination ◽  
Skim Milk ◽  
Egg Yolk ◽  
Control Group ◽  
Genetic Quality ◽  
Treatment Groups ◽  
Igf I ◽  
Complex Protein

Artificial insemination has proven to be an effective method for increasing population size and genetic quality of Kacang goats. However, innovation is required to maintain the quality of Kacang goat semen in storage. This study aimed to examine the effects of supplementing the 150 kDa protein assumed as IGF-I complex derived from bull seminal plasma in skim milk-egg yolk extender on the quality of Kacang goat sperm stored at 5°C. Twelve ejaculates collected from three Kacang goats were divided into three groups. In the control group (T0), the ejaculates were extended with skim milk-egg yolk only. In the treatment groups (T1 and T2), the ejaculates were extended with skim milk-egg yolk supplemented with the IGF-I complex protein at 12 μg and 24 μg/100 mL, respectively. The extended semen was stored at 5°C, and the viability, motility, intactness of the plasma membrane, malondialdehyde concentration, and apoptotic sperm percentage were evaluated daily for five days. The results showed that the T1 was the most effective treatment for maintaining Kacang goat semen at a quality acceptable for artificial insemination over five days of storage at 5°C. However, the T0 and T2 groups retained acceptable qualities for only three days at 5°C. It could be concluded that supplementation of 12 μg of the 150 kDa protein derived from bull seminal plasma per 100 mL extender successfully extended the life span of Kacang goat sperm for five days.

Semen quality of Merino rams. 1. The effects of fleece length and season on semen quality

1965 ◽  
Vol 5 (18) ◽  
pp. 243
Author(s):  
DG Fowler
Keyword(s):  
Semen Quality ◽  
Control Group ◽  
Monthly Variation ◽  
Summer Period ◽  
High Quality ◽  
Semen Characteristics

One group of 11 medium wool Peppin Merino rams was kept in short wool during summer 1960-61 (treatment). A second group remained unshorn during this period (control). Semen was collected from all rams at 14-day intervals during the summer period, October 1960 until March 1961, and from control rams until 243 March 1962. Both groups of rams had semen of similar and high quality during summer 1960-61. In the control group there was significant monthly variation for all semen characteristics studied but semen quality remained generally high. Significant differences were found between individual rams for all semen characteristics studied, some rams having markedly degenerate semen during the summer periods.

scholarly journals Effect of Selenium and Vitamin E Supplementation on Semen Quality in Dogs with Lowered Fertility

2015 ◽  
Vol 59 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Anna Domosławska ◽  
Sławomir Zduńczyk ◽  
Wojciech Niżański ◽  
Andrzej Jurczak ◽  
Tomasz Janowski
Keyword(s):  
Vitamin E ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Control Group ◽  
Organic Selenium ◽  
Healthy Dogs ◽  
Manual Manipulation ◽  
Motility Parameters

Abstract Thirty clinically healthy dogs with poor semen quality were used in the study. Fifteen dogs were supplemented daily with selenium (0.6 mg/kg organic selenium from yeast) and vitamin E (5 mg/kg) per os for 60 d. The control group (15 dogs) was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated with a Hamilton Thorne sperm analyser, version IVOS 12.3. For the assessment of sperm morphology, Diff-Quik stain was used. The percentage of live and dead spermatozoa was estimated on dried smears stained with eosin-nigrosin. The concentration of spermatozoa, most motility parameters determined (PMOT, VSL, VCL, ALH, BCF, RAPID, MEDIUM, SLOW, and STATIC), and the percentage of spermatozoa morphologically normal and live increased significantly (P < 0.05) after 60 d of supplementation. In the control group, there were no changes in motility parameters while the concentration and total sperm count decreased over the duration of the study. In conclusion, supplementation with selenium and vitamin E for 60 d can improve the quality of semen in dogs with lowered fertility.

scholarly journals A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

2016 ◽  
Vol 85 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Marina Anastasia Karageorgiou ◽  
Georgios Tsousis ◽  
Constantin M. Boscos ◽  
Eleni D. Tzika ◽  
Panagiotis D. Tassis ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Medium Term ◽  
Semen Collection ◽  
Chromatin Integrity ◽  
Boar Semen ◽  
Short Time

The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16–18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research.

scholarly journals Preparation of dahi from cow and buffalo skim milk with addition of mango (Mangifera indica) juice

2017 ◽  
Vol 3 (2) ◽  
pp. 191-197
Author(s):  
Sawpna Rani Biswas ◽  
Sumya Kibria ◽  
Md Rezwanul Habib ◽  
Mohammad Shohel Rana Siddiki ◽  
Md Harun Ur Rashid
Keyword(s):  
Mangifera Indica ◽  
Skim Milk ◽  
Viable Count ◽  
Control Group ◽  
Total Viable Count ◽  
Control Groups ◽  
Microbiological Test ◽  
Overall Acceptability ◽  
Mango Juice

The aim of this research was evaluation of dahi prepared from cow and buffalo skim milk addition of mango juice. Four types of dahi were prepared from cow’s and buffalo’s skim milk which designated by A (cows skim milk), B (cows skim milk with 10% mango juice), C (buffalo’s skim milk) and D (buffalo’s skim milk with 10% mango juice). The quality of prepared dahi was measured by organoleptic, chemical and microbiological test. Smell and taste, body and consistency, colour and texture score of dahi improved due to adding mango juice to compare with their control group. The overall acceptability score of B and D sample were 85.67±1.53 and 81.67±4.04 which higher than both control group. From chemical analysis, it was found higher total solids in 10% mango juice containing B (202.0±0.20 g/kg) and D (196.0±0.49 g/kg) dahi samples than both control groups. Again, lower fat and protein contents found in 10% mango juice containing B and D dahi samples. Total viable count was found significantly higher in both 10% mango juice containing B and D dahi samples than both control groups. In conclusion, that addition of mango juice with cow’s skim milk dahi or buffalo skim milk dahi were both better in terms of organoleptic and chemical qualities more acceptable than plain skim milk dahi.Asian J. Med. Biol. Res. June 2017, 3(2): 191-197

scholarly journals Effect of dietary supplementation with rocket salad (Eruca sativa) seeds powder on certain seminal plasma traits of Hy – line laying breeder roosters subjected to oxidative stress induced by hydrogen peroxide

2012 ◽  
Vol 36 (0A) ◽  
pp. 62-69
Author(s):  
Hazim J. Al – Daraji
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Seminal Plasma ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Control Group ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

This study was conducted to evaluate the effect of adding different levels of rocket salad seeds powder to the diet on seminal plasma traits of roosters subjected to oxidative stress induced by hydrogen peroxide. A total of 60 Hy – line laying breeder roosters 57 weeks old were used in this study. Roosters were randomly distributed into 5 treatments with 3 replicates each. Each replicate constituted of 4 roosters (12 roosters for each treatment). Experimental treatments were as following: T1: Males fed control diet and normal water, T2: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.25 ml hydrogen peroxide (0.5%) / litter of water, T3: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.5 ml hydrogen peroxide (0.5%) / litter of water, T4: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 1 ml hydrogen peroxide (0.5%) / litter of water, and T5: Males fed control diet and drink tap water supplemented with 1 ml hydrogen peroxide (0.5%) / litter of water. Males were treated with hydrogen peroxide (6%) and rocket salad for 12 weeks starting from 59 week of male ages. Results revealed that treated the roosters with hydrogen peroxide without adding rocket salad powder to the diet of these roosters (T5) resulted in highly significant (p< 0.01) decrease as regards concentrations of phospholipids, cholesterol, glutathione, the activity of superoxide desmutase and catalase, and total antioxidant activity in seminal plasma and highly significant (p< 0.01) increase concerning concentrations of tyrosine and malondialdehyde as compared with control group (T1) and rocket salad powder treatments (T2, T3, T4) after 12 weeks of experiment. However, supplementing diet of roosters with rocket salad powder (T2, T3, T4) resulted in highly significant (p< 0.01) increase with relation to concentrations of phospholipids, cholesterol, glutathione, the activity of superoxide desmutase and catalase, and total antioxidant activity in seminal plasma and highly significant (p< 0.01) decrease respecting concentrations of tyrosine and malondialdehyde as compared with (T5) In conclusion adding rocket salad powder to the diet of roosters had important role in limiting the negative effect of oxidative stress induced by hydrogen peroxide on seminal plasma quality of roosters. Therefore, dietary supplementation with rocket salad powder could be used as one of important tools for improving semen quality of roosters.

scholarly journals An Evaluation of Semen Quality of Donor Bulls in the Central Artificial Insemination Station (CAIS), Kundasale, Sri Lanka

2021 ◽  
Vol 32 (4) ◽  
pp. 426
Author(s):  
W. W. Abeygunawardana ◽  
P. G. A. Pushpakumara ◽  
H. M. S. Wasana ◽  
W. M. T. D. Rathnakumara ◽  
B. Alexander
Keyword(s):  
Sri Lanka ◽  
Artificial Insemination ◽  
Semen Quality

scholarly journals Effect of adding Manganese chloride and Co-enzymes (α-lipoic acid and Q10) on post-cryopreservation semen quality characteristics of Holstein bulls

2018 ◽  
Vol 41 (2) ◽  
pp. 86-93
Author(s):  
Eidan S. M.
Keyword(s):  
Lipoic Acid ◽  
Semen Quality ◽  
Manganese Chloride ◽  
Control Group ◽  
Control Groups ◽  
Holstein Bulls ◽  
Animal Resource ◽  
Fresh Semen ◽  
Artificial Vagina

     This study was undertaken to explore the adding effect of manganese chloride (MnCl2), co-enzyme Q10 (Co-Q10) as well as α-lipoic acid to Tris extender on Freeze ability of Holstein bulls’ semen. This study was carried out at the department of artificial insemination at the Directorate of Animal Resource, Ministry of Agriculture in Abu-Ghraib, Baghdad for the duration from October\ 2013 to Jun\ 2014, including three experiments. Seven Holstein bulls of 3.5-4 years old were used in this study. Semen was collected via artificial vagina by one ejaculate/ bull/ week. The assessments were conducted for fresh semen, which was later pooled, equally divided for various treatments within each experiment, using Tris extender. In the first experiment, pooled semen was divided into three groups. First group was diluted with Tris only. Manganese chloride was added to Tris extender (0.7 mM) in the 2nd group while in 3rd group (0.9 mM) of the Manganese chloride was used. In the second experiment, semen was divided equally into three groups. The first group was considered as a control group diluted with Tris only. Co-enzyme Q10 was added to 2nd (0.2 mM) and 3rd groups (0.5 mM) treatments respectively. In the third experiment, semen was divided into three groups. The first group was diluted with Tris only (control group). While the 2nd and 3rd groups were added 0.5 and 1.0 mM α-lipoic acid respectively. The effect of these additions on Holstein bulls semen quality was studied during different periods (48 hours, one, two and three months post cryopreservation) for three experiments. The results revealed that the addition two levels of the MnCl2 (2nd and 3rd, experiment 1), Co- Q10  (2nd and 3rd, experiment 2) and α- lipoic acid (2nd and 3rd experiment 3) led to significant increases freezability as compared with control groups during all the experiment periods. In conclusion, the addition of MnCl2, Co-Q10 and α-lipoic acid led to improved post-cryopreservation semen quality of Holstein bulls. This will in turn enhance fertility rate of artificially-inseminated cows and owners economic income consequently.

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