Microsatellite analysis of genetic diversity and population structure of Jordanian barley (Hordeum vulgare L.) reveals genetic polymorphism and divergence associated with inflorescence type

2018 ◽  
Vol 19 (1) ◽  
pp. 86 ◽  
Author(s):  
Maen Hasan ◽  
Nidal Odat ◽  
Issam Qrunfleh ◽  
Yahya Shakhatreh ◽  
Sobhia Saifan
Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 118
Author(s):  
Ljiljana Brbaklić ◽  
Dragana Trkulja ◽  
Sanja Mikić ◽  
Milan Mirosavljević ◽  
Vojislava Momčilović ◽  
...  

Determination of genetic diversity and population structure of breeding material is an important prerequisite for discovering novel and valuable alleles aimed at crop improvement. This study’s main objective was to characterize genetic diversity and population structure of a collection representing a 40-year long historical period of barley (Hordeum vulgare L.) breeding, using microsatellites, pedigree, and phenotypic data. The set of 90 barley genotypes was phenotyped during three growing seasons and genotyped with 338 polymorphic alleles. The indicators of genetic diversity showed differentiation changes throughout the breeding periods. The population structure discriminated the breeding material into three distinctive groups. The principal coordinate analysis grouped the genotypes according to their growth habit and row type. An analysis of phenotypic variance (ANOVA) showed that almost all investigated traits varied significantly between row types, seasons, and breeding periods. A positive effect on yield progress during the 40-year long breeding period could be partly attributed to breeding for shorter plants, which reduced lodging and thus provided higher yield stability. The breeding material revealed a considerable diversity level based on microsatellite and phenotypic data without a tendency of genetic erosion throughout the breeding history and implied dynamic changes in genetic backgrounds, providing a great gene pool suitable for further barley improvement.


2020 ◽  
Vol 33 (2) ◽  
pp. 115-124
Author(s):  
Maha R. Khalil ◽  
Hussain A. Almahasneh ◽  
Salam Y. Lawand

Investigation was carried out at the laboratory of Biotechnology, Faculty of Agriculture, Damascus University, during the season 2017-2018. Seven varieties were planted to determine the degree of genetic similarity using SSR-technique (Simple Sequence Repeats), and 14 double primers were used for this purpose. The analysis results revealed that all primers showed polymorphism among the evaluated varieties, except Bmag0385. primers produced a total of 42 alleles with a polymorphic percentage of 88.27%. The number of alleles for each primer varied from 1 allele for the primer (Bmac0067) to 7 alleles for the primer (Bmag0006) in average of 3 alleles per primer. Cluster analysis and Dendrogram showed the highest degree of genetic similarity between variety Arabi asuad  and variety Arabi abiad (0.7619). While it was low between variety Fourat4 and variety Arabi abiad (0.3571), and varieties Fourat4 and Fourat3 (0.3571) which indicated wide genetic diversity among them.


2022 ◽  
Vol 101 (1) ◽  
Author(s):  
Allo A. Dido ◽  
M. S. R. Krishna ◽  
Ermias Assefa ◽  
Dawit T. Degefu ◽  
B. J. K. Singh ◽  
...  

2020 ◽  
Vol 7 (12) ◽  
pp. 144-161
Author(s):  
Allo A. Dido ◽  
◽  
Kassahun Tesfaye ◽  
M.S.R. Krishna ◽  
Dawit T. Degefu ◽  
...  

Landraces play a key role in crop breeding by providing beneficial trait for improvement of related crops and their genetic diversity studies are very important for breeding program and identification of parental lines. In this study, 585 barley (Hordeum vulgare L.) landraces collected from 13 agro-ecological zones of Ethiopia were evaluated along with 10 cultivars for their phenotypic diversity and population structure in relation to agronomic traits, resistance to major diseases and barley shoot fly. Data on 22 agronomic traits, three major diseases and barley shoot fly resistance-related traits were recorded. Univariate and multivariate approaches such as principal component and cluster analyses were applied to assess the genetic diversity and population structure. The analysis of variance indicated significant genotypic main, accessions x year and accession x environment interaction effects for almost all the traits evaluated. However, the accessions x environment interactions were mainly due to changes in magnitude rather than crossover types of interactions. The diversity analysis indicated that the population was highly structured according to kernel row-type, region (geographic) origin and altitude classes. Since the population is highly structured, appropriate statistical models will be needed when this population is used for association mapping studies. Eight principal components (PCs) in principal component analysis (PCA) accounted for the variation of 83.01%. The most related traits were included in the same PC, implying that results from PCA could give clues as to the relationship among traits. Though variability existed within and among clusters, useful germplasm clustered together. These materials are important sources of germplasm for the improvement of agronomic, disease and insect pest resistance traits. Keywords: Barley, diseases, genetic diversity, landraces, multivariate, shoot fly


2016 ◽  
Vol 339 (11-12) ◽  
pp. 454-461 ◽  
Author(s):  
Ammar Elakhdar ◽  
Mohamed Abd EL-Sattar ◽  
Khairy Amer ◽  
Assma Rady ◽  
Toshihiro Kumamaru

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