scholarly journals Genetic profiling of bacteria belongs to genus Pseudomonas, what affects legumes

2018 ◽  
Vol 22 ◽  
pp. 120-125
Author(s):  
L. A. Dankevich
Keyword(s):  
Species Identification ◽  
Pseudomonas Syringae ◽  
Causative Agent ◽  
Genetic Heterogeneity ◽  
Molecular Phylogenetics ◽  
Molecular Genetic ◽  
Close Similarity ◽  
Genetic Profiling ◽  
Typical Representative

Aim. For the purpose of correct inter species identification and estimation of group’s heterogeneity, the genome fingerprinting of isolated by us and collection "Pseudomonas lupini" strains as well as typical representative of genus, affecting legumes, has been carried out. Methods. In the course of research, microbiological, molecular genetic (REP-PCR) methods and method of molecular phylogenetics (UPGMA) were used. Results. The genetic heterogeneity of isolated and collections "Pseudomonas lupini" strains has been estimated. A relationship between isolated and collections "Pseudomonas lupini" strains with the typical Pseudomonas syringae strains, affecting legumes, for BOX, REP and ERIC profiles has been determined. Conclusions. BOX, ERIC and REP-profiling of the genome of the agent of lupines’ brown spottiness revealed significant genetic heterogeneity of its population (from 20 to 50 % of heterogeneity) and close similarity of this pathogen to representatives of the species Pseudomonas syringae.Keywords: identification, genetic heterogeneity, REP-PCR, causative agent of lupines’ brown spottiness.

scholarly journals REP-PCR analisis of single agent of cucumber bacterial diseases

2017 ◽  
Vol 15 (1) ◽  
pp. 25-31
Author(s):  
L. A. Dankevych
Keyword(s):  
Species Identification ◽  
Statistical Methods ◽  
Genetic Heterogeneity ◽  
Significant Relationship ◽  
Molecular Genetic ◽  
Single Agent ◽  
Genetic Homogeneity ◽  
Bacterial Diseases ◽  
Typical Strain ◽  
Correct Species Identification

Aim. For the purpose of correct species identification and estimation of population’s heterogeneity, the fingerprinting of the genome of isolated by us Pectobacterium sp., collection «Erwinia toxica» strains and typical representatives of certain species of Pectobacterium and Diskeya genera has been carried out. Methods. In the course of research, microbiological, molecular genetic (REP-PCR), mathematical-statistical methods of research were used. Results. On the basic of BOX, REP and ERIC profiles the significant affinity between isolated Pectobacterium sp. and collections «Erwinia toxica» strains with the typical P. carotovorum susp. carotovorum UCM B1075T has been established. Genetic heterogeneity of isolated Pectobacterium sp. and collections «Erwinia toxica» strains has been estimated. Conclusions. It has been found the significant relationship between isolates Pectobacterium sp. and the collection «Erwinia toxica» strains with the typical strain P. carotovorum susp carotovorum UCM B1075T on the basic of their BOX, REP and ERIC profiles. Most likely, this indicates that they belong to this species. The genetic homogeneity of isolated Pectobacterium sp. strains of and the genetic heterogeneity of the collection «Erwinia toxica» strains is probably due to the plant’s selection from similar or different region.Keywords: identification, genetic heterogeneity, REPPCR, «Erwinia toxica», Pectobacterium sp.

The Leucine-Rich Repeat Domain Can Determine Effective Interaction Between RPS2 and Other Host Factors in Arabidopsis RPS2-Mediated Disease Resistance

Genetics ◽  
2001 ◽  
Vol 158 (1) ◽  
pp. 439-450 ◽  
Author(s):  
Diya Banerjee ◽  
Xiaochun Zhang ◽  
Andrew F Bent
Keyword(s):  
Disease Resistance ◽  
Pseudomonas Syringae ◽  
Plant Disease Resistance ◽  
Effective Interaction ◽  
Molecular Genetic ◽  
Defense Responses ◽  
Host Factors ◽  
Leucine Rich Repeat ◽  
Domain Swap ◽  
Allele Specific

Abstract Like many other plant disease resistance genes, Arabidopsis thaliana RPS2 encodes a product with nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains. This study explored the hypothesized interaction of RPS2 with other host factors that may be required for perception of Pseudomonas syringae pathogens that express avrRpt2 and/or for the subsequent induction of plant defense responses. Crosses between Arabidopsis ecotypes Col-0 (resistant) and Po-1 (susceptible) revealed segregation of more than one gene that controls resistance to P. syringae that express avrRpt2. Many F2 and F3 progeny exhibited intermediate resistance phenotypes. In addition to RPS2, at least one additional genetic interval associated with this defense response was identified and mapped using quantitative genetic methods. Further genetic and molecular genetic complementation experiments with cloned RPS2 alleles revealed that the Po-1 allele of RPS2 can function in a Col-0 genetic background, but not in a Po-1 background. The other resistance-determining genes of Po-1 can function, however, as they successfully conferred resistance in combination with the Col-0 allele of RPS2. Domain-swap experiments revealed that in RPS2, a polymorphism at six amino acids in the LRR region is responsible for this allele-specific ability to function with other host factors.

scholarly journals Revisiting the Issue of the Molecular-genetic Structure of the Causative Agent of the Bovine Leukemia Virus in the Russian Federation

2016 ◽  
Vol 9 (42) ◽  
Author(s):  
Irina Mikhailovna Donnik ◽  
Maksim Valeryevich Petropavlovsky ◽  
Anna Sergeevna Krivonogova ◽  
Irina Alekseevna Shkuratova ◽  
Marzena Rola-Łuszczak ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Russian Federation ◽  
Causative Agent ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Molecular Genetic ◽  
The Russian Federation

scholarly journals Genetic profiling of mistletoe (Viscum album L.) using RAPD-analysis

2020 ◽  
Vol 26 ◽  
pp. 82-86
Author(s):  
Yu. O. Bilonozhko ◽  
A. N. Rabokon ◽  
A. S. PostovoitovA ◽  
L. O. Kalafat ◽  
S. M. PrivAlikhin ◽  
...  
Keyword(s):  
Rapd Markers ◽  
Rapd Analysis ◽  
Molecular Genetic ◽  
Viscum Album ◽  
Dna Fragments ◽  
Coniferous Species ◽  
Genetic Profiling ◽  
Random Amplification ◽  
Polymerase Chain ◽  
Genetic Profiles

Aim. The aim of this research was genetic profiling and identification of genetic differences between V. album speciments, growing on deciduous and coniferous species of woody plants using RAPD markers. Methods. The method of polymerase chain reaction (PCR) with random primers (Random Amplification of Polymorphic DNA - RAPD) was used. Amplified DNA fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using the staining with silver nitrate. Results. All the studied mistletoe samples were differentiated from each other, and their unique molecular genetic profiles were obtained. 241 amplified DNA fragments were detected in the range from 200 to 2000 bp, 152 fragments (63%) were polymorphic. The samples were divided into two separate groups depending on the type of host plant. Conclusions. The fact that the samples formed two separate clades confirms the assumption that mistletoe, which grow on pine and grow on maple, represents two separate subspecies of V. album. Keywords: Viscum album L., molecular genetic markers, polymorphism, RAPD.

scholarly journals ATPS-43POTENTIAL ROLE OF MOLECULAR GENETIC PROFILING TEST FOR THE TREATMENT OF PRIMARY AND SECONDARY CNS MALIGNANCIES

2015 ◽  
Vol 17 (suppl 5) ◽  
pp. v27.4-v27
Author(s):  
Lyndon Kim ◽  
James Evans ◽  
Kevin Judy ◽  
Christopher Farrell ◽  
David Andrews
Keyword(s):  
Molecular Genetic ◽  
Genetic Profiling ◽  
Cns Malignancies

MOLECULAR GENETIC PROFILING OF GLEASON GRADE 4/5 PROSTATE CANCERS COMPARED TO BENIGN PROSTATIC HYPERPLASIA

2001 ◽  
Vol 166 (6) ◽  
pp. 2171-2177 ◽  
Author(s):  
THOMAS A. STAMEY ◽  
JANET A. WARRINGTON ◽  
MITCHELL C. CALDWELL ◽  
ZUXIONG CHEN ◽  
ZHENBIN FAN ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Molecular Genetic ◽  
Prostatic Hyperplasia ◽  
Gleason Grade ◽  
Genetic Profiling ◽  
Prostate Cancers

scholarly journals Personalized oncology as a global uniting aim of clinical experience, unified protocols and modern technologies on the way to the victory against neoplastic processes

2020 ◽  
pp. 53-54
Author(s):  
O.V. Herasymenko
Keyword(s):  
Decision Making ◽  
Growth Rate ◽  
Personalized Medicine ◽  
Molecular Genetic ◽  
Individual Characteristics ◽  
Genetic Characteristics ◽  
Genetic Profiling ◽  
Diagnostic Measures ◽  
Treatment And Prevention ◽  
Personalized Oncology

Background. According to the prognoses, by 2025 the global cancer incidence will increase to 24 million cases per year, and mortality – up to 16 million cases. The annual growth rate of tumors number exceeds the growth rate of global population, which justifies the feasibility of improving the diagnosis, treatment and prevention of these diseases. Objective. Describe the main features of personalized oncology. Materials and methods. Analysis of regulatory documents and literature sources on this topic. Results and discussion. In their work Ukrainian oncologists are guided by the domestic documents and the documents of international societies. Personalization of treatment involves taking into account such characteristics of the disease as its stage, morphological affiliation of cells and molecular genetic characteristics of the tumor, as well as such patient characteristics as sex, age, general somatic status, comorbidities, and place of residence. Personalized medicine is a set of methods for diagnosis, treatment and prevention of pathological conditions, based on the individual characteristics of the patient (genetic, epigenetic, transcriptomic, proteomic, metagenomic markers, phenotypic features). For the introduction of personalized medicine into oncology it is necessary to use the molecular genetic profiling of the tumor, to assess the risks with the help of several specialists, to involve the patient in decision-making regarding treatment and diagnostic measures. The main tools for the widespread introduction of personalized medicine include qualified specialists, laboratories of pharmacogenetics and pharmacokinetics, the formation of competencies in health care providers and the development of personalized pharmacy. The main result is the widespread implementation of treatment programs using personalized protocols with targeted delivery of the drug to the appropriate targets, taking into account the state of the body of the particular patient at the specific time. Conclusions. 1. The incidence of cancer is steadily growing. 2. Personification of treatment involves taking into account the stage of the disease, morphological affiliation of cells and molecular genetic characteristics of the tumor, as well as individual characteristics of the patient. 3. For the introduction of personalized medicine in oncology it is necessary to use molecular genetic profiling of the tumor, to assess the risks with the help of several specialists, to involve the patient in decision-making regarding treatment and diagnostic measures.

scholarly journals First Record of Trichobilharzia physellae (Talbot, 1936) in Europe, a Possible Causative Agent of Cercarial Dermatitis

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1473
Author(s):  
Nikolaus Helmer ◽  
Hubert Blatterer ◽  
Christoph Hörweg ◽  
Susanne Reier ◽  
Helmut Sattmann ◽  
...  
Keyword(s):  
Species Identification ◽  
Molecular Genetic ◽  
First Record ◽  
Integrative Taxonomy ◽  
Freshwater Snails ◽  
Cercarial Dermatitis ◽  
Close Relationship ◽  
Avian Schistosomes ◽  
First Time

Several species of avian schistosomes are known to cause dermatitis in humans worldwide. In Europe, this applies above all to species of the genus Trichobilharzia. For Austria, a lot of data are available on cercarial dermatitis and on the occurrence of Trichobilharzia, yet species identification of trematodes in most cases is doubtful due to the challenging morphological determination of cercariae. During a survey of trematodes in freshwater snails, we were able to detect a species in the snail Physella acuta (Draparnaud, 1805) hitherto unknown for Austria, Trichobilharzia physellae; this is also the first time this species has been reported in Europe. Species identification was performed by integrative taxonomy combining morphological investigations with molecular genetic analyses. The results show a very close relationship between the parasite found in Austria and North American specimens (similarity found in CO1 ≥99.57%). Therefore, a recent introduction of T. physellae into Europe can be assumed.

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