scholarly journals Identification of dehydrin genes Dhn1 and Zmdhn13 alleles in maize varieties and lines

2021 ◽  
Vol 28 ◽  
pp. 42-47
Author(s):  
M. V. Halaieva ◽  
V. I. Fait
Keyword(s):  
Zea Mays ◽  
Allele Frequency ◽  
Zea Mays L ◽  
Geographical Origin ◽  
Molecular Genetic ◽  
Chain Reaction ◽  
Maize Varieties ◽  
Polymerase Chain ◽  
Dehydrin Genes

Aim. Determination of molecular genetic polymorphism of maize varieties and lines (Zea mays L.) of Ukrainian and world selection by Dhn1 and ZmDHN13 dehydrin genes. Methods. Polymerase chain reaction (PCR), PAAgel-electrophoresis. Results. Maize varieties and lines of different geographical origin and different years of creation were analyzed by Dhn1 and ZmDhn13 dehydrin genes. Conclusions. The use of directional primers revealed five different alleles of the Dhn1 locus, which differed in the size of the amplification product: 186, 190, 194, 196 and 200 bp. The most common allele was 196 bp (46.2%). Two alleles of the ZmDhn13 locus were detected: 86 bp and 82 bp. Allele frequency 86 bp was 61.5% and the allele frequency 82 bp was 38.5%. The sequences of the dehydrin genes Dhn1 and ZmDhn13 are not conservative, deletions and / or insertions within these genes are observed. Accordingly, the structure and functional activity of the dehydrin proteins that encode these genes in different genotypes can vary greatly. Keywords: Zea mays L., dehydrins, genes, drought resistance.

Determination of the geographical origin of maize ( Zea mays L.) using mineral element fingerprints

2019 ◽  
Vol 100 (3) ◽  
pp. 1294-1300 ◽  
Author(s):  
Feng Wang ◽  
Haiyan Zhao ◽  
Chundi Yu ◽  
Juan Tang ◽  
Wei Wu ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Geographical Origin ◽  
Mineral Element

scholarly journals Genetic Variation Among Three Zea Mays L Cultivars In Iraq

2021 ◽  
Vol 910 (1) ◽  
pp. 012013
Author(s):  
Faten Khaleel ◽  
Janan saeed
Keyword(s):  
Polymerase Chain Reaction ◽  
Zea Mays ◽  
Genetic Variation ◽  
Molecular Marker ◽  
Zea Mays L ◽  
Conventional Polymerase Chain Reaction ◽  
Schematic Diagram ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Ampli Fication

Abstract Maize is a very fertile and widely environmental grown crop and it globally cultivated. The purpose of our research was to determine genetic variation among three Zea mays L. cultivars (Al maha, Drachma and Talar F-1). The primer ITS1 and ITS4 used as a molecular marker in a conventional Polymerase Chain Reaction (PCR) with a 290 bp ampli- fication outcome. The nucleotide sequences of amplification products were analyzed, sequence alignment was significantly revealed which confirming Zea mays diagnosis. Furthermore, analysis of genetic relationship revealed a neighboring relationship between Talar F-1 and Almaha cultivars(93),whereas phylogeny schematic diagram clearly showed presence of Drachma cultivar in other cluster(77).

A Study of the Efficiency of Modern Domestic Disinfectants in the System of TB Control Activities

2015 ◽  
Vol 2 (2) ◽  
pp. 26-31 ◽  
Author(s):  
A. Paliy ◽  
A. Zavgorodniy ◽  
B. Stegniy ◽  
A. Gerilovych
Keyword(s):  
Molecular Genetic ◽  
Critical Role ◽  
Bactericidal Effect ◽  
Chain Reaction ◽  
Tb Control ◽  
Source Of Infection ◽  
Polymerase Chain ◽  
And Control ◽  
Chemical Groups ◽  
Test Objects

Due to the absence of elaborated effi cient means for specifi c prevention of bovine tuberculosis, it is ex- tremely important to detect and eliminate the source of infection and to take veterinary and sanitary preven- tive measures. Here the critical role is attributed to disinfection, which breaks the epizootic chain due to the elimination of pathogenic microorganisms in the environment and involves the application of disinfectants of different chemical groups. Aim. To study the tuberculocidal properties of new disinfectants DZPT-2 and FAG against atypical mycobacteria Mycobacterium fortitum and a TB agent Mycobacterium bovis. Methods. The bacteriological and molecular-genetic methods were used. Results. It was determined that DZPT-2 prepara- tion has bactericidal effect on M. fortuitum when used in the concentration of 2.0 % of the active ingredient (AI) when exposed for 5–24 h, while disinfectant FAG has a bactericidal effect in the concentration of 2.0 % when exposed for 24 h. Disinfectant DZPT-2 in the concentration of 2.0 % of the AI, when exposed for 5–24 h, and FAG preparation in the concentration of 2.0 %, when exposed for 24 h, and with the norm of consump- tion rate of 1 cubic decimeter per 1 square meter disinfect the test-objects (batiste, wood, glazed tile, metal, glass), contaminated with the TB agent M. bovis. Conclusions. Disinfecting preparations of DZPT-2 in the concentration of 2.0 % of AI when exposed for 5 h and FAG in the concentration of 2.0 % when exposed for 24 h may be used in the complex of veterinary and sanitary measures to prevent and control TB of farm ani- mals. The possibility of using the polymerase chain reaction as an additional method of estimating tuberculo- cide activity of disinfectants was proven.

scholarly journals Identification of Associations between SSR Markers and Quantitative Traits of Maize (Zea mays L.)

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 182
Author(s):  
Jan Bocianowski ◽  
Kamila Nowosad ◽  
Barbara Wróbel ◽  
Piotr Szulc
Keyword(s):  
Zea Mays ◽  
Ssr Markers ◽  
Marker Assisted Selection ◽  
Quantitative Traits ◽  
Zea Mays L ◽  
Fresh Weight ◽  
Multiple Traits ◽  
Good Tool ◽  
Maize Varieties ◽  
Hybrid Maize

Microsatellite or simple sequence repeat (SSR) markers have wide applicability for genetic analysis in crop plant improvement strategies. Marker-assisted selection is an important tool for plant breeders to increase the efficiency of a breeding process, especially for multigenic traits, highly influenced by the environment. In this paper, the relationships between SSR markers and 26 quantitative traits of hybrid maize varieties (Zea mays L.) were analyzed. Association analyses were performed based on 30 SSR primers in a set of thirteen hybrid maize varieties. A total of 112 SSR markers were detected in these genotypes. The number of alleles per locus ranged from 1 to 17, with the average number of alleles per locus equal to 3.7. The number of molecular markers associated with observed traits ranged from 1 (for the number of kernels in row, ears weight and fresh weight of one plant) to 14 (for damage of maize caused by P. nubilalis) in 2016 as well as from 1 (for soil plant analysis development—SPAD, the number of grains in ear and fresh weight of one plant) to 12 (for carotenoids content) in 2017. The sum of statistically significant associations between SSR markers and at least one trait was equal to one hundred sixty in 2016 as well as one hundred twenty-five in 2017. Marker trait associations (MTAs) were found on the basis of regression analysis. The proportion of the total phenotypic variances of individual traits explained by the marker ranged from 24.4% to 77.7% in the first year of study and from 24.3% to 77.9% in 2017. Twenty-two SSR markers performed a significant effect on at least one tested trait in both years of experiment. The three markers (phi021/4, phi036/3, and phi061/2) can be a good tool in marker-assisted selection because they allow simultaneous selection for multiple traits in both years of study, such as the number of kernels in row and the number of grains in ear (phi021/4), the number of plant after germination, the number of plants before harvest, and the number of ears (phi036/3), as well as moisture of grain and length of ears (phi061/2).

scholarly journals Determination of water stress with spectral reflectance on sweet corn (Zea mays L.) using classification tree (CT) analysis

2013 ◽  
Vol 100 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Levent Genc ◽  
Melis Inalpulat ◽  
Unal Kizil ◽  
Mustafa Mirik ◽  
Scot E. Smith ◽  
...  
Keyword(s):  
Zea Mays ◽  
Water Stress ◽  
Spectral Reflectance ◽  
Zea Mays L ◽  
Sweet Corn ◽  
Classification Tree ◽  
Ct Analysis
Sign in / Sign up

Export Citation Format

Share Document