scholarly journals Free prolin content in Arabidopsis thaliana Cat2 and Cat3 knockout mutants under salt stress

2016 ◽  
Vol 14 (1) ◽  
pp. 3-7 ◽  
Author(s):  
N. O. Didenko ◽  
I. M. Buzduga ◽  
R. A. Volkov ◽  
I. I. Panchuk
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Sodium Chloride ◽  
Cellular Response ◽  
Resistance Mechanisms ◽  
Stress Conditions ◽  
Free Proline ◽  
Knockout Mutants ◽  
Knockout Line

Aim. In plants, the enzymatic and non-enzymatic environmental stress resistance mechanisms function in a concerted manner, but the role of specific isoforms of antioxidant enzymes and their relationship to low molecular weight protective compounds is poorly understood. To investigate this question free proline levels were compared under salt stress conditions in wild-type Arabidopsis thaliana and knockout mutants for the catalase genes Cat2 and Cat3. Methods. Free proline content was measured under various treatments of plants with sodium chloride. Results. It was shown that under salt stress conditions free proline increase is impaired in leaves of mutants lacking CAT2 and CAT3 activity. In addition, cat3 knockout line shows a reduced basal level of proline under non-stress conditions. Conclusions. Suppression of response to salt stress points to a possible link between the rearrangement of the antioxidant system in the Cat genes knockouts and the regulation of protective cellular response to salt stress.Key words: Arabidopsis thaliana, knockout mutants, catalase, proline, sodium chloride.

scholarly journals The content of polyphenolic compounds in the Arabidopsis thaliana knockout mutant Cat2 under salt stress

2016 ◽  
Vol 14 (2) ◽  
pp. 174-177
Author(s):  
N. O. Didenko ◽  
I. M. Buzduga ◽  
R. A. Volkov ◽  
I. I. Panchuk
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Sodium Chloride ◽  
Stress Responses ◽  
Stress Proteins ◽  
Polyphenolic Compounds ◽  
Knockout Mutant ◽  
Knockout Mutants ◽  
Multigenic Family

Aim. In plants, the defense response to abiotic stress includes induction of stress proteins and increase in content of protective metabolites. To date, the role of specific isoforms of antioxidant enzymes in stress responses and their relation to low-molecular weight protective compounds are still not clarified. To study this question the content of polyphenolic compounds (PPC) was evaluated under salt stress in Arabidopsis thaliana wild-type (WT) and in catalase 2 (Cat2) knockout mutant plants. Methods. PPC content in different variants of treatment with sodium chloride was measured. Results. It was shown that under optimal cultivation conditions the content of PPC in leaves of cat2 mutants is higher than in WT leaves. However, cultivation of isolated shoots in nutrient medium resulted in a faster depletion of the PPC pool in the cat2 line. Also, short-term salt stress results in equal depletion of the PPC pool in both, WT and cat2. Conclusions. The increase of PPC content in cat2 leaves is a manifestation of metabolic alterations that aim to compensate the reduced catalase activity.Keywords: multigenic family, knockout mutants, polyphenolic compounds, sodium chloride, Arabidopsis thaliana.

Flavonoid profiling by LC-MS IN 14 – 3-3ν knockout mutants of Arabidopsis thaliana during drought stress conditions

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
F Nabbie ◽  
O Shperdheja ◽  
J Millot ◽  
J Lindberg ◽  
B Peethambaran
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Conditions ◽  
Knockout Mutants

Identification of two phenotypes of Arabidopsis thaliana under in vitro salt stress conditions

2007 ◽  
Vol 51 (3) ◽  
pp. 436-442 ◽  
Author(s):  
K. B. Carrasco ◽  
R. Fornasiero ◽  
A. Tassoni ◽  
N. Bagni
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Conditions

scholarly journals ROS-Scavengers, Osmoprotectants and Violaxanthin De-Epoxidation in Salt-Stressed Arabidopsis thaliana with Different Tocopherol Composition

2021 ◽  
Vol 22 (21) ◽  
pp. 11370
Author(s):  
Ewa Surówka ◽  
Dariusz Latowski ◽  
Michał Dziurka ◽  
Magdalena Rys ◽  
Anna Maksymowicz ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Transgenic Line ◽  
Wild Type ◽  
Carbon And Nitrogen ◽  
Nacl Concentration ◽  
Tocopherol Composition ◽  
Ros Scavengers ◽  
Excessive Accumulation

To determine the role of α- and γ-tocopherol (TC), this study compared the response to salt stress (200 mM NaCl) in wild type (WT) Arabidopsis thaliana (L.) Heynh. And its two mutants: (1) totally TC-deficient vte1; (2) vte4 accumulating γ-TC instead of α-TC; and (3) tmt transgenic line overaccumulating α-TC. Raman spectra revealed that salt-exposed α-TC accumulating plants were more flexible in regulating chlorophyll, carotenoid and polysaccharide levels than TC deficient mutants, while the plants overaccumulating γ-TC had the lowest levels of these biocompounds. Tocopherol composition and NaCl concentration affected xanthophyll cycle by changing the rate of violaxanthin de-epoxidation and zeaxanthin formation. NaCl treated plants with altered TC composition accumulated less oligosaccharides than WT plants. α-TC deficient plants increased their oligosaccharide levels and reduced maltose amount, while excessive accumulation of α-TC corresponded with enhanced amounts of maltose. Salt-stressed TC-deficient mutants and tmt transgenic line exhibited greater proline levels than WT plants, lower chlorogenic acid levels, and lower activity of catalase and peroxidases. α-TC accumulating plants produced more methylated proline- and glycine- betaines, and showed greater activity of superoxide dismutase than γ-TC deficient plants. Under salt stress, α-TC demonstrated a stronger regulatory effect on carbon- and nitrogen-related metabolites reorganization and modulation of antioxidant patterns than γ-TC. This suggested different links of α- and γ-TCs with various metabolic pathways via various functions and metabolic loops.

scholarly journals Characterization of a Novel Member of the DegS-DegU Regulon Affected by Salt Stress in Bacillus subtilis

1998 ◽  
Vol 180 (7) ◽  
pp. 1855-1861 ◽  
Author(s):  
Véronique Dartois ◽  
Michel Débarbouillé ◽  
Frank Kunst ◽  
Georges Rapoport
Keyword(s):  
Bacillus Subtilis ◽  
Salt Stress ◽  
Control Region ◽  
Negative Control ◽  
Control Site ◽  
High Salt ◽  
Stress Conditions ◽  
Target Sequence ◽  
Marine Habitats

ABSTRACT As a soil bacterium also found in estuarine and marine habitats,Bacillus subtilis has evolved various sensing and adaptation systems in order to face salt stress conditions. Among these regulatory mechanisms is the DegS-DegU signal transduction system, which was previously shown to be stimulated by high salt concentrations. A search for promoters regulated in response to salt stress led to the identification of wapA, encoding a wall-associated protein, which is strongly expressed at low salt concentrations and almost completely repressed in the presence of 0.7 M disodium succinate. Repression of wapA transcription by salt stress was shown to require the phosphorylated form of DegU. Moreover, DegU-mediated repression of wapA occurred only in high-salt medium. Alignment between the control region ofwapA and other DegU-regulated promoters allowed the identification of a putative DegU target sequence, AGAAN11TTCAG. Mutation/deletion analyses of thewapA promoter region confirmed the role of the putative DegU control site in repression of wapA transcription at high salt concentrations and revealed a second site of repression located downstream from the transcription start site. Since residual negative control was observed at this second site in the absence of DegU, it seems likely that an additional repressor acts on thewapA control region to further downregulatewapA transcription under salt stress conditions.

scholarly journals Potential Role of Annexin AnnAt1 from Arabidopsis thaliana in pH-Mediated Cellular Response to Environmental Stimuli

2007 ◽  
Vol 48 (6) ◽  
pp. 792-803 ◽  
Author(s):  
Karolina M. Gorecka ◽  
Cyril Thouverey ◽  
Rene Buchet ◽  
Slawomir Pikula
Keyword(s):  
Arabidopsis Thaliana ◽  
Cellular Response ◽  
Potential Role ◽  
Environmental Stimuli

scholarly journals The endophytic fungus Piriformospora indica enhances Arabidopsis thaliana growth and modulates Na + /K + homeostasis under salt stress conditions

Plant Science ◽  
2017 ◽  
Vol 263 ◽  
pp. 107-115 ◽  
Author(s):  
Mohamed E. Abdelaziz ◽  
Dongjin Kim ◽  
Shawkat Ali ◽  
Nina V. Fedoroff ◽  
Salim Al-Babili
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Endophytic Fungus ◽  
Stress Conditions ◽  
Piriformospora Indica
Sign in / Sign up

Export Citation Format

Share Document