scholarly journals Tumour-homing chimeric polypeptide-conjugated polypyrrole nanoparticles for imaging-guided synergistic photothermal and chemical therapy of cancer

Theranostics ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 2634-2645 ◽  
Author(s):  
Mengmeng Sun ◽  
Jianwen Guo ◽  
Hanjun Hao ◽  
Tong Tong ◽  
Kun Wang ◽  
...  
Keyword(s):  
Polypyrrole Nanoparticles ◽  
Chimeric Polypeptide ◽  
Tumour Homing

scholarly journals Investigating the Disordered and Membrane-Active Peptide A-Cage-C Using Conformational Ensembles

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3607
Author(s):  
Olena Dobrovolska ◽  
Øyvind Strømland ◽  
Ørjan Sele Handegård ◽  
Martin Jakubec ◽  
Morten L. Govasli ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Protein Misfolding ◽  
Driving Forces ◽  
Conformational Space ◽  
Supported Bilayers ◽  
Conformational Ensembles ◽  
Protein Membrane ◽  
Chimeric Polypeptide ◽  
Protein Misfolding And Aggregation ◽  
In Silico Analyses

The driving forces and conformational pathways leading to amphitropic protein-membrane binding and in some cases also to protein misfolding and aggregation is the subject of intensive research. In this study, a chimeric polypeptide, A-Cage-C, derived from α-Lactalbumin is investigated with the aim of elucidating conformational changes promoting interaction with bilayers. From previous studies, it is known that A-Cage-C causes membrane leakages associated with the sporadic formation of amorphous aggregates on solid-supported bilayers. Here we express and purify double-labelled A-Cage-C and prepare partially deuterated bicelles as a membrane mimicking system. We investigate A-Cage-C in the presence and absence of these bicelles at non-binding (pH 7.0) and binding (pH 4.5) conditions. Using in silico analyses, NMR, conformational clustering, and Molecular Dynamics, we provide tentative insights into the conformations of bound and unbound A-Cage-C. The conformation of each state is dynamic and samples a large amount of overlapping conformational space. We identify one of the clusters as likely representing the binding conformation and conclude tentatively that the unfolding around the central W23 segment and its reorientation may be necessary for full intercalation at binding conditions (pH 4.5). We also see evidence for an overall elongation of A-Cage-C in the presence of model bilayers.

A novel electrically conductive and biodegradable composite made of polypyrrole nanoparticles and polylactide

Biomaterials ◽  
2004 ◽  
Vol 25 (13) ◽  
pp. 2477-2488 ◽  
Author(s):  
Guixin Shi ◽  
Mahmoud Rouabhia ◽  
Zhaoxu Wang ◽  
Lê H Dao ◽  
Ze Zhang
Keyword(s):  
Electrically Conductive ◽  
Biodegradable Composite ◽  
Polypyrrole Nanoparticles

Preparation and characterization of RGD tumour-homing-peptide-modified plasminogen K5

2010 ◽  
Vol 57 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Yang Wang ◽  
Zhongqiang Guo ◽  
Qing Ge ◽  
Enhua Wang ◽  
Xiuli Zhang ◽  
...  
Keyword(s):  
Homing Peptide ◽  
Tumour Homing

scholarly journals IMMUNOCHEMICAL ANALYSIS OF RECOMBINANT CHIMERIC POLYPEPTIDE OspCgar+afz OF BORRELIA GARINII AND B. AFZELIIISOLATES

2016 ◽  
pp. 37-44
Author(s):  
VS. .. Karavaev ◽  
E. S. Oleinikova ◽  
M. Sh. Azaev ◽  
A. B. Beklemishev'
Keyword(s):  
Recombinant Proteins ◽  
Western Siberia ◽  
West Siberia ◽  
Amino Acid Sequences ◽  
Borrelia Garinii ◽  
Test Systems ◽  
Antigenic Activity ◽  
Escherichia Coli Cells ◽  
Antigenic Properties ◽  
Chimeric Polypeptide

Aim. Comparative study of antigenic properties of recombinant proteins OspCgar and OspCafz and recombinant chimeric polypeptide OspCgar+afZ, that contains amino acid sequences of mature immune dominant OspC proteins of West-Siberian isolates of Borrelia garinii (OspCgar) and B. afzelii (OspCafz), and evaluation of possibility of their use as antigens during creation of test-systems for serodiagnostics of Lyme borreliosis (LB) on the territory of Western Siberia. Materials and methods. Recombinant chimeric polypeptide OspCgar+afz and recombinant mature proteins OspCgar and OspCafz, obtained by expression of the corresponding genes in Escherichia coli cells, purified by affinity chromatography in Ni-NTA-sepharose CL-6B and studied by EIA method for the ability to bind antibodies from sera of LB patients. Results. A difference in sensitivity of determination by EIA method of specific IgM and IgG against borreliae in blood sera of LB patients with localized stage of the disease during use of OspCgar, OspCafz and OspCgar+afZ chimera as antigens was shown. Chimeric antigen OspCgar+afz was established to show higher antigenic activity compared with each of the OspCgar or OspCafZ antigens separately. Conclusion. The results of the study allow to examine the recombinant chimeric polypeptide OspCgar+afz as a possible component during creation of test-systems for serodiagnostics of LB on the territory of West Siberia.

Enhanced photothermal therapy of biomimetic polypyrrole nanoparticles through improving blood flow perfusion

Biomaterials ◽  
2017 ◽  
Vol 143 ◽  
pp. 130-141 ◽  
Author(s):  
Xuejun Wang ◽  
Haichun Li ◽  
Xianping Liu ◽  
Ye Tian ◽  
Huishu Guo ◽  
...  
Keyword(s):  
Blood Flow ◽  
Photothermal Therapy ◽  
Flow Perfusion ◽  
Polypyrrole Nanoparticles

Ultra-low voltage triggered release of an anti-cancer drug from polypyrrole nanoparticles

Nanoscale ◽  
2018 ◽  
Vol 10 (20) ◽  
pp. 9773-9779 ◽  
Author(s):  
Devleena Samanta ◽  
Niloufar Hosseini-Nassab ◽  
Aidan D. McCarty ◽  
Richard N. Zare
Keyword(s):  
Low Voltage ◽  
Cancer Drug ◽  
Cancer Drugs ◽  
Triggered Release ◽  
Redox Active ◽  
Anti Cancer ◽  
Polypyrrole Nanoparticles

Redox-active anti-cancer drugs can be released without compromising their bioactivity from polypyrrole nanoparticles that respond to ultra-low voltages (−75 mV).

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