Proximate and Quantitative Phytochemical Analysis of Detarium microcarpum (Sweet Detar) Fruit Pulp

The current study aimed to evaluate, in vitro, the antioxidant capacity and the human lymphocyte-protective effect of the ethanolic extract from Detarium microcarpum fruit pulp against oxidative stress damage. Human lymphocytes were incubated with different concentrations of extract, followed by the addition of hydrogen peroxide or tert-butyl hydroperoxide. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the antioxidant property of the extract was evaluated in vitro using hydrogen peroxide and nitric oxide radical-scavenging assays. Compared to the vehicle, the fruit pulp ethanol extract did not exhibit a cytotoxic effect on human lymphocytes. Furthermore, the cytotoxicity of hydrogen peroxide and tert-butyl hydroperoxide to human lymphocytes was significantly reduced by fruit extract pretreatment. The extract and ascorbic acid exhibited similar cytoprotective activity (p > 0.05). The fruit pulp extract showed more antioxidant activity than gallic acid in the hydrogen peroxide-scavenging model, while in the nitric oxide-quenching model, the fruit extract and gallic acid showed similar activity. The fruit pulp of D. microcarpum contains potent antioxidant and cell-protective compounds. The use of the fruit pulp of D. microcarpum as a food supplement could rescue cellular oxidative damage responsible for numerous pathologies.

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Physicochemical Characterization, Phytochemical and HPTLC Fingerprinting Studies on Fruit of Couroupita Guianensis

Chiang Mai University Journal of Natural Sciences ◽

10.12982/cmujns.2021.078 ◽

2021 ◽

Vol 20 (4) ◽

Author(s):

Lawrence Anna Sheba ◽

◽

Anuradha Venkatraman ◽

Keyword(s):

High Performance ◽

Physicochemical Characterization ◽

Water Soluble ◽

Phytochemical Analysis ◽

Important Criterion ◽

Fruit Pulp ◽

Primary Metabolites ◽

Couroupita Guianensis ◽

Medicinal Properties

Abstract Traditional knowledge and literature studies report that each part of a plant has tremendous medicinal values. Validation of these medicinal plants scientifically is an important criterion for the development of plant-based drugs. Couroupita guianensis (Family: Lecythidaceae) is a plant with immense medicinal properties. To authenticate its biological value, the present investigation aims to standardize the fruit of C. guianensis based on physicochemical characterization, phytochemical analysis both qualitatively and quantitatively, and high-performance thin-layer liquid chromatography (HPTLC) fingerprinting studies. Fruit pulp of C. guianensis was obtained, processed and extracted with solvents such as petroleum ether, chloroform, ethanol and hydroalcohol. Moisture content, total ash, water-soluble ash and acid-insoluble ash values were calculated. Preliminary phytochemical analysis revealed the existence of several secondary metabolites in the extracts. In addition, interpreting peaks obtained from HPTLC analysis revealed the presence of potential bioactive phytoconstituents in all the extracts. The quantitative determination proclaimed that fruit pulp was found to be rich in phenolics and flavonoids followed by tannin and saponin. Further, primary metabolites were quantified and they were found to be abundant in the fruit pulp. Henceforth, the outcome of these results provides information for assessing the quality of the sample that could help in ensuring its therapeutic efficacy. Keywords: Couroupita guianensis, HPTLC, Physicochemical characterization, Phytochemical, Fingerprinting

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In Vivo Antiplasmodial Activity of Detarium microcarpum (Fabaceae) Stem Bark Extract

Thrita ◽

10.5812/thrita.116921 ◽

2021 ◽

Vol 10 (1) ◽

Author(s):

Amira Rahana Abdullahi ◽

Sani Malami ◽

Lawal Alhassan Bichi

Keyword(s):

Hematological Parameters ◽

Lethal Dose ◽

Parasitic Infection ◽

Stem Bark ◽

Antiplasmodial Activity ◽

Bark Extract ◽

Control Group ◽

Phytochemical Analysis ◽

Detarium Microcarpum ◽

Stem Bark Extract

Background: Malaria is a parasitic infection that is common in the tropical and sub-tropical regions of the world. It is estimated to kill one million individuals annually. Detarium microcarpum (Fabaceae family) is used traditionally for the treatment of malaria, diabetes, hypertension, convulsions, pneumonia, leprosy, and meningitis. The purpose of this research was to evaluate the antiplasmodial potential and safety profile of the methanol extract of D. microcarpum stem bark. Methods: The extract was subjected to phytochemical screening and oral median lethal dose (LD50) estimation. Using curative, suppressive, and prophylactic experimental animal models, antiplasmodial activity was assessed in mice infected with chloroquine-sensitive Plasmodium berghei berghei. Biochemical and hematological tests were conducted in rats orally administered with the extract for 28 days. One-way analysis of variance (ANOVA) was used to analyze the data, followed by Dunnett's post hoc test. Results: Alkaloids, flavonoids, saponins, tannins, triterpenes, and glycosides were found in the extract through phytochemical analysis. When compared to the negative control group, the extract at the tested doses (200, 400, and 800 mg/kg) showed a significant (P < 0.001) curative, suppressive, and prophylactic effect and significantly (P < 0.001) prolonged the survival time of mice in the treated groups. The extract had an insignificant (P > 0.05) effect on the biochemical and hematological parameters tested. Conclusions: The results suggest that the stem bark extract of D. microcarpum at the doses tested possesses antiplasmodial activity and is relatively safe after short-term use.

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Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from <i>Detarium microcarpum</i> Guill. and Perr. (Caesalpiniaceae)

American Journal of Biomedical and Life Sciences ◽

10.11648/j.ajbls.20180604.13 ◽

2018 ◽

Vol 6 (4) ◽

pp. 78

Author(s):

Ablassé Rouamba

Keyword(s):

Dna Repair ◽

Ethanol Extract ◽

Fruit Pulp ◽

Detarium Microcarpum

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In vitro and in vivo anti-salmonella properties of hydroethanolic extract of Detarium microcarpum Guill. & Perr. (Leguminosae) root bark and LC-MS-based phytochemical analysis

Journal of Ethnopharmacology ◽

10.1016/j.jep.2020.113049 ◽

2020 ◽

Vol 260 ◽

pp. 113049

Author(s):

Michel Arnaud Mbock ◽

William Feudjou Fouatio ◽

Raceline Gounoue Kamkumo ◽

Patrick Valère Tsouh Fokou ◽

Florence Ngueguim Tsofack ◽

...

Keyword(s):

Phytochemical Analysis ◽

Root Bark ◽

Detarium Microcarpum ◽

Hydroethanolic Extract

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PHYTOCHEMICAL ANALYSIS AND ASSESSMENT OF THE PROSPECTIVE USE OF QUINCE IN MEDICINE

Global problems of modernity ◽

10.26787/nydha-2713-2048-2020-1-10-11-12-54-57 ◽

2020 ◽

pp. 54-57

Author(s):

Sayapina A.E.

Keyword(s):

Medicinal Plant ◽

Quality Indicators ◽

Raw Materials ◽

Current Trend ◽

Phytochemical Analysis ◽

Fruit Pulp ◽

Preliminary Assessment ◽

Waste Products ◽

Plant Raw Materials ◽

Pharmaceutical Production

Taking into account the current trend of the wider application of food products and waste products of their production, it is relevant to study the possibility of using quince (Cydonia) and quince fruit pulp as medicinal plant raw materials. The scientific novelty of the research consists in the development of quality indicators of new medicinal plant raw materials, the research confirms the possibility of using the quince fruit pulp. In the course of the study, a complex of pharmacological works was carried out, as well as a preliminary assessment of the effectiveness of the implementation of waste-free technology in pharmaceutical production.

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