scholarly journals Physicochemical Characterization, Phytochemical and HPTLC Fingerprinting Studies on Fruit of Couroupita Guianensis

2021 ◽  
Vol 20 (4) ◽  
Author(s):  
Lawrence Anna Sheba ◽  
◽  
Anuradha Venkatraman ◽  
Keyword(s):  
High Performance ◽  
Physicochemical Characterization ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Important Criterion ◽  
Fruit Pulp ◽  
Primary Metabolites ◽  
Couroupita Guianensis ◽  
Medicinal Properties

Abstract Traditional knowledge and literature studies report that each part of a plant has tremendous medicinal values. Validation of these medicinal plants scientifically is an important criterion for the development of plant-based drugs. Couroupita guianensis (Family: Lecythidaceae) is a plant with immense medicinal properties. To authenticate its biological value, the present investigation aims to standardize the fruit of C. guianensis based on physicochemical characterization, phytochemical analysis both qualitatively and quantitatively, and high-performance thin-layer liquid chromatography (HPTLC) fingerprinting studies. Fruit pulp of C. guianensis was obtained, processed and extracted with solvents such as petroleum ether, chloroform, ethanol and hydroalcohol. Moisture content, total ash, water-soluble ash and acid-insoluble ash values were calculated. Preliminary phytochemical analysis revealed the existence of several secondary metabolites in the extracts. In addition, interpreting peaks obtained from HPTLC analysis revealed the presence of potential bioactive phytoconstituents in all the extracts. The quantitative determination proclaimed that fruit pulp was found to be rich in phenolics and flavonoids followed by tannin and saponin. Further, primary metabolites were quantified and they were found to be abundant in the fruit pulp. Henceforth, the outcome of these results provides information for assessing the quality of the sample that could help in ensuring its therapeutic efficacy. Keywords: Couroupita guianensis, HPTLC, Physicochemical characterization, Phytochemical, Fingerprinting

THE EFFECT OF PROCESSING METHODS ON THE NUTRITIONAL QUALITY OF AFRICAN BREADFRUITS (TRECULIA AFRICANA) SEEDS

2020 ◽  
Vol 16 (2) ◽  
pp. 60
Author(s):  
Nwozo Sarah Onyenibe ◽  
Julius Oluwaseun Oluwafunmilola ◽  
Stanley Udogadi Nwawuba
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Nutritional Quality ◽  
High Performance ◽  
Phytochemical Analysis ◽  
Mineral Contents ◽  
Processing Methods ◽  
African Breadfruit

The extracted seeds of African breadfruit are identified to be extremely healthy whenever it is correctly processed. Therefore, the aim of the present study was to evaluate the effects of processing methods on the nutritional quality of African breadfruit seed. A qualitative phytochemical analysis including: Alkaloid, Flavonoid, Saponin, Tannin, Anthraquinone, Terpenoids, Steroid, and Cardiac Glycosides for the different fraction of African breadfruit seed was performed using a standard method. The result revealed the presence and greater amount of phytochemical for the raw fraction; seven in eight, six in eight for steamed fraction, and four in eight for boiled and roasted respectively. Anti-nutrient, Proximate, and Mineral Content were also conducted using standard methods. The amino acid composition was determined using High-Performance Liquid Chromatography (HPLC). The results of the present study revealed that anti-nutrients including Phytate, Tannins, and Oxalate were significantly p<0.05 reduced in the boiled fraction 5.47±0.15, 3.42±0.02 and 6.89±0.05, and highest in the raw fraction 7.77±0.01, 5.09±0.03 and 9.34±0.14. The proximate composition including; percentage crude fat, Ash, Carbohydrate, Fatty acid, and Energy value were significantly lower p<0.05 in the boiled fraction relative to the other fractions. Mineral contents; calcium, magnesium, sodium, potassium, and phosphorus were also significantly p<0.05 elevated in the boiled fraction relative to the raw, steamed, and roasted fraction. The amino acid composition was highest in the roasted and boiled fraction 57.350 and 56.978, and lowest in the steamed and raw fraction 35.754 and 28.748 respectively. Therefore, boiling (cooking) is encouraged for the preparation of African breadfruit seed.

scholarly journals High-performance Acetone Soluble Tape Transfer Printing Method for Heterogeneous Integration

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jiaqi Zhang ◽  
Yichang Wu ◽  
Zhe Li ◽  
Yachao Zhang ◽  
Yue Peng ◽  
...  
Keyword(s):  
Sapphire Substrate ◽  
High Performance ◽  
Water Soluble ◽  
Experimental Results ◽  
Heterogeneous Integration ◽  
Transfer Printing ◽  
Adhesion Promoter ◽  
Thermal Release ◽  
Printing Method

Abstract A high-performance transfer printing method using a new soluble tape which can be dissolved in acetone is proposed to be used in heterogeneous integration. Si inks array was transferred from SOI wafers onto various substrates without adhesion promoter by this new method which we refer to as the acetone soluble tape (AST) method to compare with other transfer printing methods by using thermal release tape (TRT), water soluble tape (WST) and polydimethylsiloxane (PDMS). By using the AST method, the transfer printing process does not involve interface contention between stamp/inks and inks/receiver substrate so that it maximizes the transfer printing efficiency. Experimental results present the AST method has good performances, and various alien substrates, even curvilinear surfaces, can be selected as receiver substrates by the AST method. To examine the quality of the transferred Si inks, the Si TFTs were fabricated by using the Si membrane transferred by the AST method on sapphire substrate and the devices show the good performance. All the results confirm that the AST method is an effective method in heterogeneous integration.

scholarly journals PHYSICOCHEMICAL AND PHYTOCHEMICAL ANALYSIS OF Mangifera indica linn.( AAMRA KERNEL )

2020 ◽  
Vol p4 (05) ◽  
pp. 2394-2400
Author(s):  
Pooja Dogra ◽  
A. Rama Murthy ◽  
Sumit Nathani
Keyword(s):  
Mangifera Indica ◽  
Natural Antioxidants ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Tropical Regions ◽  
Potential Source ◽  
Mango Kernel ◽  
Phytochemical Constituents ◽  
Phytochemical Studies

Introduction: Mangifera Indica Linn., Anacardiaceae family, is a large evergreen tree of tropical and sub-tropical regions of the world. Mango kernel can be used as a potential source for functional food ingredi-ents, animal feeds, antimicrobial compounds and cosmetic due to its high quality of fat and protein as well as high levels of natural antioxidants. Aim: The aim of the present study was to evaluate the physicochemical and phytochemical analysis of Mangifera Indica Linn. Methods Aamra Kernel were collected, cleaned, dried and powdered. Aqueous and alcoholic extracts of Aamra kernel were analyzed for physicochemical and phytochemical constituents. The study was conduct-ed as per the guidelines of Ayurvedic pharmacopeia of India. Results: The result of physicochemical parameters (moisture content: 10.2%, total ash: 2.12%, acid insolu-ble ash: 0.3%, water soluble ash: 1.42%, aqueous extractive value: 14.67% and alcoholic extractive value 12.98%). The phytochemical studies revealed the presence of active constituents, carbohydrates, amino acid, steroids, proteins, saponins, alkaloids, glycosides, tannins in aqueous and alcoholic extracts. Conclusion: All studies standardization parameters like physicochemical and phytochemical screening pa-rameters provide the knowledge in the identification, authentication of kernel of Mangifera Indica Linn. Hence it is concluded that this drug supposed to be great beneficial and boon for our society.

scholarly journals Fingerprint Study of Curcuma xanthorrhiza Rhizome by High Performance Thin Layer Chromatography (HPTLC)

2016 ◽  
Vol 1 (2) ◽  
pp. 9-14
Author(s):  
Tina Wikara ◽  
Anny Sulistiowaty ◽  
Sri Murhandini ◽  
Tepy Usia
Keyword(s):  
High Performance ◽  
Phytochemical Analysis ◽  
Hptlc Plate ◽  
Spot Detection ◽  
Ich Guidelines ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography ◽  
Chloroform Methanol

The rhizome of Curcuma xanthorrhiza Roxb is intensively used in Indonesia as traditional medicine. It is widely used for hepatoprotective and anti inflammatory activities. To ensure the quality of its extract, we have studied the fingerprint or phytochemical analysis. This research was aimed to produce a chromatogram profile of the rhizome by HPTLC. The HPTLC fingerprint chromatogram of C. xanthorrhiza rhizome was performed using HPTLC plate of silica gel 60 F254 as the stationary phase and chloroform-methanol (97:3) as the mobile phase. Spot detection was carried out by TLC photo documentary system at 254 and 366 nm and TLC scanner at 427 nm. The developed method was validated according to ICH guidelines by determination of specificity and precision. We found that the specifity and precission of the method were met the acceptance criteria. In conclusion, the developed method is valid and could be used for quality control and standardization of herbal medicine containing C. xanthorrhiza rhizome.

scholarly journals Phyto-Pharmacognostical Standardization with HPTLC Fingerprinting of Root of Citraka (Plumbago Zeylanica L.)

2021 ◽  
pp. 20-33
Author(s):  
Akhilraj A.R ◽  
Rukmini S ◽  
Amalraj A.R
Keyword(s):  
Medicinal Plant ◽  
Research Work ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Plumbago Zeylanica ◽  
Light Yellow ◽  
Physico Chemical ◽  
The Family ◽  
Medicinal Properties ◽  
Analytical Standards

Background: Citraka or Sveta Citraka (Plumbago zeylanica L.) is an important medicinal plant mentioned in Ayurvedic classics, belongs to the family Plumbaginaceae. The root of the plant exhibits medicinal properties. It has a broad range of pharmacological activities, including anti-inflammatory, antibacterial, antifungal, anticancer, antidiabetic, antioxidant, hepatoprotective, wound healing and cytotoxic. Objective: The aim of the study was to evaluate phyto-pharmacognostical standardization with HPTLC fingerprinting of root of P. zeylanica, one of the most accepted and beneficial medicinal plant in Ayurveda. Materials and Methods: P. zeylanica was collected from Konni, Kerala, India during the month of May 2018 and its root was studied for macroscopic, microscopic, physico-chemical, preliminary phytochemical screening and HPTLC fingerprinting following the standard protocol recommended by The Ayurvedic Pharmacopoeia of India and Quality Standards of Indian Medicinal Plants. Results: The root is long, stout, cylindrical and reddish to dark brown in colour. The transverse section of root showed 5-7 rows of small cubical or rectangular celled cork with light yellow walls, polygonal or slightly tangentially elongated cortex cells containing starch grains and coloured contents, phloem containing small groups of fibres, radially arranged xylem vessels and narrow, 1-4 seriate medullary rays. Physico-chemical analysis showed water soluble extractive value as 26.11±0.01 w/w and alcohol soluble extractive value as 15.85±0.00 w/w. Preliminary phytochemical analysis of root decoction showed the presence of alkaloids, carbohydrates, tannins, saponins, resin and quinones. Conclusion: The information obtained from this research work may be useful to establish the botanical as well as analytical standards for the root of P. zeylanica.

scholarly journals The PHARMACOGNOSTICAL, PHYSICOCHEMICAL AND HIGH-PERFORMANCE THIN LAYER CHROMATOGRAHY (HPTLC) EVALUATION OF ERANDAMULADI KALKA CHOORNA

2020 ◽  
Vol 9 (5) ◽  
Author(s):  
Swathi N. ◽  
Anup B. Thakar ◽  
Harisha C.R. ◽  
V.J. Shukla
Keyword(s):  
Pharmaceutical Analysis ◽  
High Performance ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Low Back ◽  
Soluble Extract ◽  
Physico Chemical ◽  
Common Ailment ◽  
Back Ache ◽  
Water Soluble Extract

Introduction: Erandamuladi Niruha Basti is mentioned in Charaka Samhita which is indicated in Trika, Prishta Shoola (low back ache) and acts as Maruta Nigraha. Low backache is the most common ailment in today’s busy life. Erandamuladi Kalka used in Basti contains 9 drugs in which most of the drugs are having Ushna Veerya and are Shoolahara in nature. Method: Sookshma Choorna of Erandamuladi Kalka was evaluated for their pharmacognostic and pharmaceutical analysis. Results: Pharmacognostic study showed the presence of contents such as cluster crystal of Hapusha, starch grains of Musta, spiral vessels of Bala etc. Physico-chemical analysis showed that the loss on drying 6.28%, Ash value 13.26%, Water soluble extract 19.34%, Alcohol soluble extract 4.24%, pH 7.0 Discussion and Conclusion: The pharmacognostical and phytochemical analysis of Erandamuladi Kalka Choorna confirmed the purity and genuinity of drug, which can be espoused for laying down the standards of it. Keywords: Erandamuladi Kalka Choorna, Pharmacognostic, Phytochemical analysis, HPTLC

scholarly journals EXTRACTION OF FLAVONOID FROM VARIOUS PARTS OF COUROUPITAGUIANENSIS AND ITS EFFICACY AGAINST PATHOGENIC BACTERIA

2017 ◽  
Vol 10 (4) ◽  
pp. 354 ◽  
Author(s):  
Hemalatha S ◽  
Tahira Akther ◽  
Mohd Shahanbaj Khan
Keyword(s):  
Antibacterial Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Analysis ◽  
Phytochemical Analysis ◽  
Plant Parts ◽  
Couroupita Guianensis ◽  
Wide Range ◽  
Different Parts

Objective: Quantitative, qualitative, high performance liquid chromatography (HPLC) analysis of flavonoids content from different parts of Couroupita guianensis and antibacterial activity against various pathogenic bacteria.Methods: Different parts of C. guianensis such as leaves, stem bark, flower anther, flower petals, fruit rind, and fruit pulp were collected and used for extraction  of active constituents. The phytochemical analysis was conducted to identify the presence of flavonoids in different plant parts. The amount of flavonoids were analyzed by quantitative analysis. Furthermore, HPLC analysis was performed to isolate and identify the flavonoids from the different parts. The methanol extract of different plant parts was also used to test the antibacterial efficacy in different human bacterial pathogens.Results: Flavonoids demonstrate a wide range of biochemical and pharmacological effects. In this study, we identified the flavonoids content in different parts of C. guianensis. The HPLC analysis has significantly proved the presence of flavonoids in different plants parts and amount of flavonoids differs in different parts of the plant. The results also showed the significant efficacy of its extract to the different pathogenic bacterial strains.Conclusion: Our study suggested that C. guianensis is a richer source of flavonoids and due to a higher amount of flavonoids; it may have enormous potential to scavenge the free radicals, oxidative damage of cell during different stresses including bacterial infections. We also suggested that HPLC analysis is efficient method to isolate and identify the different compounds and study can further extend to identify and isolate the different novel compounds from C. guianensis.Keywords: Couroupita guianensis, High performance liquid chromatography, Flavonoids, Antibacterial activity.

scholarly journals PHARMACOGNOSTICAL AND PHYTOCHEMICAL STUDY ON LEAF OF COMBRETUM INDICUM (L.)

2021 ◽  
Vol 12 (5) ◽  
pp. 13-16
Author(s):  
Jyothi Jose ◽  
Subrahmanya P ◽  
Ravi Rao S
Keyword(s):  
Vascular Bundle ◽  
Transverse Section ◽  
Glandular Trichome ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Calcium Oxalate Crystals ◽  
Phytochemical Study ◽  
Starch Grains ◽  
Medicinal Properties ◽  
Endodermal Layer

Combretum indicum (L.) is an extra-pharmacopeial drug with an abundance of medicinal properties. Throughout the world, different Parts of Combretum indicum (L.) is used in curing many ailments by folklore healers. In India, the drug Combretum indicum (L.) is commonly known by the name MadhuMalathi and used by the folklore healers of different areas. Especially the use of Combretum indicum (L.) is seen among the folklore Practitioners of Dakshina Kannada District in curing ailments including paediatric conditions. Thus, it is very important to explore the identity of this drug by the proper Pharmacognostical and Phytochemical analysis. Hence, the present study was aimed at Pharmacognostical and Phytochemical study on Leaf of Combretum indicum (L.). The transverse section of the midrib of the leaf shows the presence of the endodermal layer; which is single-layered, surrounds by a vascular bundle and packed with starch grains. Endodermis covers the vascular bundle and contains several starch grains. The powder microscopy shows the presence of glandular trichome, calcium oxalate crystals etc. The percentage of extractive value was maximum in methanol which was 7.18%, Preliminary phytochemical study showed the presence of Proteins, Carbohydrates, Tannins, Steroids, Alkaloids, Triterpenoids, Starch, Resin and Phenols. The total ash attained was 7.84% and the water-soluble ash was 3.4%.

scholarly journals Fingerprint Study of Curcuma xanthorrhiza Rhizome by High Performance Thin Layer Chromatography (HPTLC)

2016 ◽  
Vol 1 (2) ◽  
pp. 9-14 ◽  
Author(s):  
Tina Wikara ◽  
Anny Sulistiowaty ◽  
Sri Murhandini ◽  
Tepy Usia
Keyword(s):  
High Performance ◽  
Phytochemical Analysis ◽  
Hptlc Plate ◽  
Spot Detection ◽  
Ich Guidelines ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography ◽  
Chloroform Methanol

The rhizome of Curcuma xanthorrhiza Roxb is intensively used in Indonesia as traditional medicine. It is widely used for hepatoprotective and anti inflammatory activities. To ensure the quality of its extract, we have studied the fingerprint or phytochemical analysis. This research was aimed to produce a chromatogram profile of the rhizome by HPTLC. The HPTLC fingerprint chromatogram of C. xanthorrhiza rhizome was performed using HPTLC plate of silica gel 60 F254 as the stationary phase and chloroform-methanol (97:3) as the mobile phase. Spot detection was carried out by TLC photo documentary system at 254 and 366 nm and TLC scanner at 427 nm. The developed method was validated according to ICH guidelines by determination of specificity and precision. We found that the specifity and precission of the method were met the acceptance criteria. In conclusion, the developed method is valid and could be used for quality control and standardization of herbal medicine containing C. xanthorrhiza rhizome.

scholarly journals Physicochemical characterization and microbiology quality of the Pentadesma butyracea fruit pulp collected from various parks in Benin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bernolde Paul Ayegnon ◽  
Ifagbémi Bienvenue Chabi ◽  
Folachodé Ulrich Gildas Akogou ◽  
Adéchola Pierre Polycarpe Kayodé
Keyword(s):  
Average Length ◽  
Physicochemical Characterization ◽  
Chemical Characterization ◽  
Titratable Acidity ◽  
Physical Aspect ◽  
Fruit Pulp ◽  
Crude Lipid ◽  
Post Harvest Loss

AbstractIn Benin, P. butyracea fruit pulp undergoes significant post-harvest loss due to its low valorization. The collected fruits in five parklands from a survey of transformer's perception were characterized through their visual observations, the determination of their dimensions and pulp proportions. The nutritional value of pulp was evaluated through its chemical characterization. The results of this study revealed that four shapes (ellipsoid, globular, ovoid, ellipsoid twisted) identify the physical aspect of P. butyracea fruits in the P. butyracea parklands. The average length, diameter at the equator, mass and pulp proportion of fruits were 130.93 mm, 86.98 mm, 125.63 g and 35.79%, respectively. The physicochemical characterization showed that the P. butyracea fruit pulp contained 3.37–3.41 pH, 3.33–4.61% protein, 20.37–20.78% fiber, 2.78–3.57% ash, 6.31–6.51% crude lipid, 85.77–86.47% moisture, 21.54–23.22 mg/mL total phenolics, 0.73–1.03% titratable acidity, 65.10–66.94% carbohydrates, 335.75–338.99 kcal calorie and minerals (Ca, Fe, Zn, Mn). The antioxidant activity result showed that the P. butyracea fruit pulp is a valuable source of antioxidant pigments. Faecal coliforms are not detected in pulps and the aerobic mesophilic bacteria, yeasts and moulds, and Staphylococcus aureus were below detection level in P. butyracea fruit pulps.

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