ALKALINE PHOSPHATASE ENZYME AND LACTATE DEHYDROGENASE ACTIVITY IN URINE OF PATIENTS TREATED WITH METHOTREXATE

In order study methotrexate nephrotoxicity, the activities of proximal tubule epithelial cell membrane enzymes: alkaline phosphatase (AP) and lactate dehydrogenase (LDH) were determined in 12-h-urine samples of 30 patients with lymphoblastomous leukemia. The patients were i.v. receiving 4 individual methotrexate doses of 2000 mg/m2 every 15 days followed by leucovorin as a protector. Control and methotrexate-treated group, each consisting of 30 examinees, included 4-10 years old children of both sexes. Statistically significant increase of AP and LDH activities, expressed as unuts/mmol creatinine was observed after the second therapy (p0.05) in relation to the control. Based on these results it can concluded that nephrotoxic methotrexate action is ireversible during the time period after the second applications at the level of proximal tubule epithelal cell.

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ACTIVITIES OF PROXIMAL TUBULE ENZYMES AND ALBUMIN CONCENTRATION IN URINE OF CHILDREN TREATED WITH METHOTREXATE

Contemporary Materials ◽

10.7251/comen1501074v ◽

2015 ◽

Vol 6 (1) ◽

Author(s):

Tatjana Vujić ◽

Biljana Davidović Plavšić ◽

Snežana Uletilović ◽

Svjetlana Stoisavljević Šatara ◽

Jelica Predojević Samardžić ◽

...

Keyword(s):

Epithelial Cell ◽

Proximal Tubule ◽

Statistical Significance ◽

Treated Group ◽

Urinary Albumin ◽

Albumin Concentration ◽

Cell Organelles ◽

Control Value ◽

The Third ◽

Proximal Tubule Epithelial Cell

In order to study methotrexate nephrotoxicity, the activities of proximal tubule epithelial cell membrane enzymes: alanine aminopeptidase (AAP) and gamma-glutamyltransferase (GGT), as well as of lysosomal N-acetyl-beta-D-glucosaminidase (NAG) and urinary albumin concentrations were determined in 12-h-urine samples of 30 patients with lymphoblastomous leukemia. The patients were i.v. receiving 4 individual methotrexate doses of 2000 mg/m2 every 15 days followed by leucovorin as a protector. Control and methotrexate-treated group, each consisting of 30 examinees, included 4–10 years old children of both sexes. Statistically significant increase of AAP and GGT activities, expressed as U/mmol creatinine was observed after the first two (p 0.05), as well as after the remaining two therapies (p 0.01) in relation to the control. Enzymatic activities of these two enzymes decreased to control value before the second and the third methotrexate application, but they increased again after the third application and remained elevated up to the end of experiments. Significant increase of NAG activity expressed as U/mmol creatinine (p 0.01), as well as urinary albumin levels (mg/mmol creatinine; p 0.01) were registered after the third methotrexate therapy and this elevation of the same statistical significance of the differences remained stable till the end of the therapy. Based on these results it can be concluded that during the time period of two first applications nephrotoxic methotrexate action is reversible and at the level of proximal tubule epithelial cell membranes. During the last two applications impairment is irreversible and at the level of cell organelles and glomerular filtration.

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Blood Biomarkers of Recovery Efficiency in Soccer Players

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16183279 ◽

2019 ◽

Vol 16 (18) ◽

pp. 3279 ◽

Cited By ~ 5

Author(s):

Anna Nowakowska ◽

Dorota Kostrzewa-Nowak ◽

Rafał Buryta ◽

Robert Nowak

Keyword(s):

Alkaline Phosphatase ◽

Creatine Kinase ◽

Lactate Dehydrogenase ◽

Alanine Aminotransferase ◽

Soccer Players ◽

Control Group ◽

Iron Level ◽

Recovery Efficiency ◽

Lactate Dehydrogenase Activity ◽

Over Time

Physical exercise strongly affects human metabolism and causes biochemical changes. This study aimed to investigate the relationship between routine plasma biomarker levels and recovery efficiency in soccer players during an entire competitive match season. The players participating in the study were divided into a midfielder/defender group (seven midfielders and seven defenders) and a goalie/substitute group (six persons—goalkeepers and players with a short cumulative match-time). The fasting capillary blood samples were taken 17–24 h after each competitive match. The blood plasma was used to determine the creatinine, urea, alkaline phosphatase, creatine kinase, lactate dehydrogenase, aspartate and alanine aminotransferase, iron and magnesium levels of the athletes. The levels of (AST) (aspartate aminotransferase), (ALT) (alanine aminotransferase) and (Cr) creatinine were higher in the midfielder/defender group than in the control group, but only AST and Cr significantly varied over time (AST decreased, and Cr increased with time). The (LDH) (lactate dehydrogenase) activity and urea level were significantly lower in the midfielder/defender group than in the goalie/substitute group, and it significantly varied over time (LDH decreased, and urea increased with time). No differences in the (CK) creatine kinase and (ALP) alkaline phosphatase activities between the groups was found, although CK increased significantly with time in the midfielder/defender group (particularly midfielders in the spring round). In midfielders, the AST activity and the iron level were significantly lower in the spring than in the autumn round. On the contrary, ALT, CK, urea and magnesium levels were significantly higher in the spring than in autumn round. A long-term measurement of biochemical parameters in elite soccer players indicated that AST, CK, LDH and creatinine levels, when analyzed together, could constitute a useful set of markers for monitoring recovery periods.

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HK-2: An immortalized proximal tubule epithelial cell line from normal adult human kidney

Kidney International ◽

10.1038/ki.1994.6 ◽

1994 ◽

Vol 45 (1) ◽

pp. 48-57 ◽

Cited By ~ 587

Author(s):

Michael J. Ryan ◽

Gretchen Johnson ◽

Judy Kirk ◽

Sally M. Fuerstenberg ◽

Richard A. Zager ◽

...

Keyword(s):

Epithelial Cell ◽

Proximal Tubule ◽

Cell Line ◽

Human Kidney ◽

Normal Adult ◽

Epithelial Cell Line ◽

Adult Human ◽

Proximal Tubule Epithelial Cell

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Cadmium alters the formation of benzo[a]pyrene DNA adducts in the RPTEC/TERT1 human renal proximal tubule epithelial cell line

Toxicology Reports ◽

10.1016/j.toxrep.2014.07.003 ◽

2014 ◽

Vol 1 ◽

pp. 391-400 ◽

Cited By ~ 12

Author(s):

Bridget R. Simon ◽

Mark J. Wilson ◽

Diane A. Blake ◽

Haini Yu ◽

Jeffrey K. Wickliffe

Keyword(s):

Epithelial Cell ◽

Proximal Tubule ◽

Cell Line ◽

Dna Adducts ◽

Renal Proximal Tubule ◽

Epithelial Cell Line ◽

Proximal Tubule Epithelial Cell

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Hepatic stem cells and their enzymatic markers- A review

Agricultural Reviews ◽

10.18805/ag.r-1775 ◽

2018 ◽

Author(s):

Amit Kumar ◽

Sourabh Sulabh

Keyword(s):

Stem Cells ◽

Alkaline Phosphatase ◽

Lactate Dehydrogenase ◽

Progenitor Cells ◽

Ethical Issues ◽

Fetal Liver ◽

Lactate Dehydrogenase Activity ◽

Liver Stem Cells ◽

Enzymatic Markers

Stem cells are those cells which show capacity for self-renewal and ability to give rise to multiple differentiated cellular populations. Enzymatic activity, as a marker for cell proliferation and cell viability, is used by metabolic activity assays. Liver stem cells/progenitor cells can be a useful source of liver treatment. They can repopulate and restore injured liver. Fetal liver stem/ progenitor cells have been found to be more capable in this, but are subjected to ethical issues. Adult liver stem cells and stem cells from animals can be used. Alkaline phosphatase and lactate dehydrogenase are enzymatic markers of in vitro hepatocyte culture. During in vitro cell culture, in the culture medium, secreted alkaline phosphatase activity increases during exponential growth of cells, whereas low extracellular lactate dehydrogenase activity indicates increased number of viable cells. Alkaline phosphatase and lactate dehydrogenase activities can be used to assess hepatocytes proliferation in vitro.

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