scholarly journals In vitro health beneficial activities of Pumpkin seeds from Cucurbita moschata cultivated in Lemnos

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Danai Sakka ◽  
Haralabos C. Karantonis
Keyword(s):  
Platelet Activating Factor ◽  
Health Improvement ◽  
Cucurbita Moschata ◽  
Pumpkin Seed ◽  
Anti Oxidant ◽  
Pumpkin Seeds ◽  
Anti Inflammatory ◽  
Locally Grown ◽  
Seed Extracts

Pumpkin seeds are commonly consumed in Greece. Although Cucurbita moschata is locally grown in Lemnos and is traditionally used in pumpkin pies, the seeds are currently discarded after consumption of the fruit flesh. The aim of the present study was to investigate the nutritional functionality of pumpkin seeds from Cucurbita moschata grown in Lemnos.Cucurbita moschatas’ seeds, raw or roasted, were appropriately extracted and the results are presented for raw versus (vs) roasted seed extracts. The phenolic content was expressed as μg gallic acid/g of seeds according to Folin-Ciocalteau assay (370.3 ± 19.1 vs 551.0 ± 22.0). Antioxidant capacity was expressed as equivalent amount for 50% scavenging in mg of seeds for DPPH (50.03 ± 5.91 vs 25.82 ± 6.77) and ABTS (17.85 ± 0.77 vs 12.77 ± 0.76) assays, and as μmol of trolox/g of seeds for FRAP (1.19 ± 0.05 vs 2.50 ± 0.23) and CUPRAC (2.13 ±0.11 vs 3.25 ± 0.06) assays. Anti-inflammatory/anti-thrombotic and anti-diabetic activities were expressed as mg of seeds for 50% inhibition of platelet activating factor (0.62 vs 0.15) and as μg of seeds for 25% inhibition of alpha-glycosidase (40.0 vs 61.0) activities respectively. Moreover, anti-atherogenic activity was expressed as the % increase in lag time of human plasma oxidation (62.7 versus 163.2)Raw and roasted pumpkin seed extracts exert anti-oxidant, anti-thrombotic/anti-inflammatory, anti-atherogenic and antidiabetic activities. Cucurbita moschata seeds may represent a novel opportunity for development of functional foods, with a local interest in Lemnos that would contribute also to the regional public health improvement.

scholarly journals In Vitro Effects of Pumpkin (Cucurbita moschata) Seed Extracts on Echinococcus granulosus Protoscoleces

2020 ◽  
Author(s):  
Zahra HESARI ◽  
Meysam SHARIFDINI ◽  
Mohammad Kazem SHARIFI-YAZDI ◽  
Saeedeh GHAFARI ◽  
Sara GHASEMI ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Echinococcus Granulosus ◽  
Cucurbita Moschata ◽  
Total Extract ◽  
Hydroalcoholic Extract ◽  
Organic Extract ◽  
Pumpkin Seeds ◽  
In Vitro Effects ◽  
Seed Extracts

Background: Echinococcus granulosus parasite causes a zoonotic disease which is important for public and veterinary health. Since pumpkin seeds (Cucurbita sp.) are used as traditional vermifuge in Iran, they may be a potential herbal anthelmintic. Methods: This study was designed in 2016 to evaluate the in vitro scolicidal effect of Cucurbita moschata seeds form northern part of Iran. Hydroalcoholic and petroleum ether extracts were prepared by maceration and soxhlet respectively. Both extracts with four different concentrations (100, 10, 1, 0.1 mg/ml) were incubated against protoscoleces in 5, 15, 30 and 60 min. Results: Maximum mortality was 16% with 1% hydroalcoholic extract in 60 min. The highest mortality with organic extract was 4% with 10% concentration in 60 min (P=0.015). Conclusion: Since highest mortality was 16%, the extract did not reach to LD50 (50% mortality). Therefore, the potency of the total extract is not sufficient as potential scolicidal drug.

scholarly journals Bioactive Compound Analysis of Coriandrum Sativum L Against Microbial Keratitis

Ophthalmology ◽  
2018 ◽  
pp. 268-282
Author(s):  
V. Nithya
Keyword(s):  
Multiple Drug Resistance ◽  
Bioactive Compound ◽  
Coriandrum Sativum ◽  
Multiple Drug ◽  
Herbal Drug ◽  
Fungal Populations ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Seed Extracts

In recent years, multiple drug resistance has been developed due to indiscriminate use of existing drugs in the treatment of infectious diseases. One such herbal drug is Coriandrum Sativum L that possesses potential pharmaceutical activities and used in several ayurvedic formulations. Keratitis caused by bacteria, viruses, fungi and parasites. The main goal of this study is to evaluate the antibacterial, antifungal activity, anti-oxidant activity and anti-inflammatory activity of Coriandrum Sativum L leaves and seeds using various organic solvent of plant extract against keratitis disease. The present study carried out the effect on various organic solvents extract of leaves and seeds of Corindrum sativum not only on the bacterial and fungal populations isolated from the conjunctivitis infected eyes but also to screen the phytochemical, anti-inflammatory and in vitro antioxidant potential of the leaf and seed extracts.

Bioactive Compound Analysis of Coriandrum Sativum L against Microbial Keratitis

2017 ◽  
pp. 109-125
Author(s):  
V. Nithya
Keyword(s):  
Multiple Drug Resistance ◽  
Bioactive Compound ◽  
Coriandrum Sativum ◽  
Multiple Drug ◽  
Herbal Drug ◽  
Fungal Populations ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Seed Extracts

In recent years, multiple drug resistance has been developed due to indiscriminate use of existing drugs in the treatment of infectious diseases. One such herbal drug is Coriandrum Sativum L that possesses potential pharmaceutical activities and used in several ayurvedic formulations. Keratitis caused by bacteria, viruses, fungi and parasites. The main goal of this study is to evaluate the antibacterial, antifungal activity, anti-oxidant activity and anti-inflammatory activity of Coriandrum Sativum L leaves and seeds using various organic solvent of plant extract against keratitis disease. The present study carried out the effect on various organic solvents extract of leaves and seeds of Corindrum sativum not only on the bacterial and fungal populations isolated from the conjunctivitis infected eyes but also to screen the phytochemical, anti-inflammatory and in vitro antioxidant potential of the leaf and seed extracts.

scholarly journals Anthelmintic effects of pumpkin (Cucurbita moschata) seed extract on Ascaris suum in vitro

2017 ◽  
Vol 10 (1) ◽  
pp. 1-6
Author(s):  
SHITA GANESTYA ◽  
SUTARMIADJI DJUMARGA ◽  
CR. SITI UTARI
Keyword(s):  
Ascaris Suum ◽  
Sampling Technique ◽  
Seed Extract ◽  
Cucurbita Moschata ◽  
Spearman Correlation ◽  
Pumpkin Seed ◽  
Death Time ◽  
Significant Difference ◽  
Seed Extracts

Ganestya S, Djumarga S, Utari CRS. 2012. Anthelmintic effects of pumpkin (Cucurbita moschata) seed extract on Ascaris suum in vitro. Biofarmasi 10: 1-6. The aim of this research was to determine the effect of pumpkin seed extract towards the Lethal Death Time of Ascaris suum Goeze in vitro. The type of this research was an quasi-experiment using a post-test only controlled group design. The subject of this research was the actively motile adult Ascaris suum Goeze. A purposive sampling technique was used to collect the samples by considering the length of each worm without noticing the sex of worm. The subjects were divided into four groups, each group consisted of 10 worms, with 5 times replication. Physiological saline solution and pyrantel pamoate 0.236% were used as the negative and positive control groups, respectively, while the pumpkin seed extracts used for the treatment groups were at the concentrations of 54.5% and 70.5%. The observation and checking towards the dead worm were performed once an hour. The data obtained were analyzed by using a Kruskal-Wallis test, continued with Mann-Whitney and Spearman Correlation tests. The Lethal Death Time of A. suum by pumpkin seed extracts at a concentration of 54.5% was 11 hour 48 minutes, while at a concentration of 70.5%, the Lethal Death Time was 7 hours 48 minutes. The result of Kruskal-Wallis test showed a significant difference among all of the groups (p<0.05). A significant difference was also shown at every group by Mann-Whitney test (p<0.05). Meanwhile, the result of Spearman correlation test showed a significant negative correlation between the concentration of pumpkin seed extract and the Lethal Death Time as p<0.05 and coefficient correlation was -0.950. Pumpkin seed extract showed an anthelmintic effect against A. suum in vitro.

Red Grape Polyphenol Oral Administration improves Immune Response in Women Affected by Nickel-Mediated Allergic Contact Dermatitis

2020 ◽  
Vol 20 ◽  
Author(s):  
Thea Magrone ◽  
Emilio Jirillo ◽  
Manrico Magrone ◽  
Matteo Antonio Russo ◽  
Paolo Romita ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Oral Administration ◽  
Allergic Contact Dermatitis ◽  
Laboratory Data ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Red Grape ◽  
Allergic Contact ◽  
Drop Outs

Background: Our previous findings demonstrated that in vitro supplementation of polyphenols, extracted from seeds of red grape (Nero di Troia cultivar), to peripheral lymphomonocytes from patients affected by allergic contact dermatitis (ACD) to nickel (Ni) could reduce release of pro-inflammatory cytokines and nitric oxide (NO), while increasing levels of interleukin (IL)-10, an anti-inflammatory cytokine. Objective: To assess whether an intervention with oral administration of polyphenols leads to a reduction of peripheral biomarkers in ACD patients. Method: At T0, 25 patients affected by ACD to Ni were orally administered with 300 mg polyphenols prodie extracted from seeds of red grape (Nero di Troia cultivar) (NATUR-OX®) for 3 months (T1). Other 25 patients affected by ACD to Ni received placebo only for the same period of time. Serum biomarkers were analyzed at T0 and T1. In both groups seven drop outs were recorded. Result: At T1 in comparison to T0, in treated patients, values of IFN-γ, IL-4, IL-17, PTX3 and NO decreased, while IL-10 levels increased when compared with T0 values. Conversely, in placebo-treated patients no modifications of biomarkers were evaluated at T1. Conclusion: Present laboratory data rely on the anti-oxidant, anti-inflammatory and anti-allergic properties of polyphenols.

scholarly journals Bioactive Lipids of Marine Microalga Chlorococcum sp. SABC 012504 with Anti-Inflammatory and Anti-thrombotic Activities

Marine Drugs ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 28
Author(s):  
Katie Shiels ◽  
Alexandros Tsoupras ◽  
Ronan Lordan ◽  
Constantina Nasopoulou ◽  
Ioannis Zabetakis ◽  
...  
Keyword(s):  
Essential Fatty Acids ◽  
Lipid Fraction ◽  
Platelet Activating Factor ◽  
Human Platelets ◽  
Bioactive Molecules ◽  
Bioactive Lipids ◽  
Alternative Source ◽  
Lipid Fractions ◽  
Anti Inflammatory

Microalgae are at the start of the food chain, and many are known producers of a significant amount of lipids with essential fatty acids. However, the bioactivity of microalgal lipids for anti-inflammatory and antithrombotic activities have rarely been investigated. Therefore, for a sustainable source of the above bioactive lipids, the present study was undertaken. The total lipids of microalga Chlorococcum sp., isolated from the Irish coast, were fractionated into neutral-, glyco-, and phospho-lipids, and were tested in vitro for their anti-inflammatory and antithrombotic activities. All tested lipid fractions showed strong anti-platelet-activating factor (PAF) and antithrombin activities in human platelets (half maximal inhibitory concentration (IC50) values ranging ~25–200 μg of lipid) with the highest activities in glyco- and phospho-lipid fractions. The structural analysis of the bioactive lipid fraction-2 revealed the presence of specific sulfoquinovosyl diacylglycerols (SQDG) bioactive molecules and the HexCer-t36:2 (t18:1/18:1 and 18:2/18:0) cerebrosides with a phytosphingosine (4-hydrosphinganine) base, while fraction-3 contained bioactive phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules. These novel bioactive lipids of Chlorococcum sp. with putative health benefits may indicate that marine microalgae can be a sustainable alternative source for bioactive lipids production for food supplements and nutraceutical applications. However, further studies are required towards the commercial technology pathways development and biosafety analysis for the use of the microalga.

scholarly journals EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITY OF AQUEOUS AND METHANOLIC SEED EXTRACTS OF CITRULLUS LANATUS

2017 ◽  
Vol 9 (5) ◽  
pp. 29
Author(s):  
Iswariya S. ◽  
Uma T. S.
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Aqueous Extract ◽  
Seed Extract ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Anti Inflammatory ◽  
Seed Extracts

Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.

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