Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development

During animal ontogenesis, body axis patterning is finely regulated by complex interactions among several signaling pathways. Nitric oxide (NO) and retinoic acid (RA) are potent morphogens that play a pivotal role in vertebrate development. Their involvement in axial patterning of the head and pharynx shows conserved features in the chordate phylum. Indeed, in the cephalochordate amphioxus, NO and RA are crucial for the correct development of pharyngeal structures. Here, we demonstrate the functional cooperation between NO and RA that occurs during amphioxus embryogenesis. During neurulation, NO modulates RA production through the transcriptional regulation of Aldh1a.2 that irreversibly converts retinaldehyde into RA. On the other hand, RA directly or indirectly regulates the transcription of Nos genes. This reciprocal regulation of NO and RA pathways is essential for the normal pharyngeal development in amphioxus and it could be conserved in vertebrates.

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Crosstalk between Nitric Oxide and Retinoic Acid pathways is essential for amphioxus pharynx development

10.1101/2020.06.22.164632 ◽

2020 ◽

Author(s):

F Caccavale ◽

G Annona ◽

L Subirana ◽

H Escriva ◽

S Bertrand ◽

...

Keyword(s):

Nitric Oxide ◽

Transcriptional Regulation ◽

Retinoic Acid ◽

Signaling Pathways ◽

The Other ◽

Vertebrate Development ◽

Reciprocal Regulation ◽

Axial Patterning ◽

Response Elements ◽

Complex Interactions

AbstractDuring animal ontogenesis, body axis patterning is finely regulated by complex interactions between several signaling pathways. Nitric Oxide (NO) and Retinoic Acid (RA) are potent morphogens that play a pivotal role in vertebrate development. Their involvement in axial patterning of head and pharynx shows conserved features in the chordate phylum. Indeed, in the cephalochordate amphioxus NO and RA are crucial for the correct development of pharyngeal structures. Here we demonstrate the functional cooperation between NO and RA occurring in amphioxus embryogenesis. During neurulation, NO modulates RA production through the transcriptional regulation of Aldh1a.2 that irreversibly converts retinaldehyde into RA. On the other hand, RA regulates the transcription of Nos genes, probably through RA Response Elements found in their regulatory regions. The reciprocal regulation of NO and RA pathways results to be essential for the normal pharyngeal development in amphioxus and suggests that this regulatory crosstalk could be conserved in vertebrates.

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Correlational Methods for Analysis of Dance Movements

Dance Research ◽

10.3366/drs.2011.0021 ◽

2011 ◽

Vol 29 (supplement) ◽

pp. 283-304 ◽

Cited By ~ 8

Author(s):

Timothy R. Brick ◽

Steven M. Boker

Keyword(s):

Motion Capture ◽

Mirror Symmetry ◽

Cross Correlation ◽

The Other ◽

Free Form ◽

Motion Capture Data ◽

Complex Interactions ◽

Dance Movements ◽

Time Lagged ◽

Insight Into

Among the qualities that distinguish dance from other types of human behavior and interaction are the creation and breaking of synchrony and symmetry. The combination of symmetry and synchrony can provide complex interactions. For example, two dancers might make very different movements, slowing each time the other sped up: a mirror symmetry of velocity. Examining patterns of synchrony and symmetry can provide insight into both the artistic nature of the dance, and the nature of the perceptions and responses of the dancers. However, such complex symmetries are often difficult to quantify. This paper presents three methods – Generalized Local Linear Approximation, Time-lagged Autocorrelation, and Windowed Cross-correlation – for the exploration of symmetry and synchrony in motion-capture data as is it applied to dance and illustrate these with examples from a study of free-form dance. Combined, these techniques provide powerful tools for the examination of the structure of symmetry and synchrony in dance.

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Faculty Opinions recommendation of Transcriptional Regulation Contributes to Prioritized Detoxification of Hydrogen Peroxide over Nitric Oxide.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.735709074.793568903 ◽

2019 ◽

Author(s):

Robert Poole

Keyword(s):

Nitric Oxide ◽

Hydrogen Peroxide ◽

Transcriptional Regulation

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The Signaling Pathways in Nitric Oxide Production by Neutrophils Exposed to N-nitrosodimethylamine

Letters in Drug Design & Discovery ◽

10.2174/1570180815666180426121503 ◽

2018 ◽

Vol 16 (2) ◽

pp. 194-199

Author(s):

Wioletta Ratajczak-Wrona ◽

Ewa Jablonska

Keyword(s):

Nitric Oxide ◽

Signaling Pathways ◽

Molecular Mechanisms ◽

Polymorphonuclear Neutrophils ◽

Microbial Pathogens ◽

Human Neutrophils ◽

No Production ◽

Oxide Synthase ◽

Inducible Nitric Oxide

Background: Polymorphonuclear neutrophils (PMNs) play a crucial role in the innate immune system’s response to microbial pathogens through the release of reactive nitrogen species, including Nitric Oxide (NO). </P><P> Methods: In neutrophils, NO is produced by the inducible Nitric Oxide Synthase (iNOS), which is regulated by various signaling pathways and transcription factors. N-nitrosodimethylamine (NDMA), a potential human carcinogen, affects immune cells. NDMA plays a major part in the growing incidence of cancers. Thanks to the increasing knowledge on the toxicological role of NDMA, the environmental factors that condition the exposure to this compound, especially its precursors- nitrates arouse wide concern. Results: In this article, we present a detailed summary of the molecular mechanisms of NDMA’s effect on the iNOS-dependent NO production in human neutrophils. Conclusion: This research contributes to a more complete understanding of the mechanisms that explain the changes that occur during nonspecific cellular responses to NDMA toxicity.

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Transcriptional regulation of carbonic anhydrase II by retinoic acid in the human pacreatic tumor cell line DANG

FEBS Letters ◽

10.1016/0014-5793(95)00596-2 ◽

1995 ◽

Vol 368 (1) ◽

pp. 45-48 ◽

Cited By ~ 11

Author(s):

Stefan Rosewicz ◽

Ernst-Otto Riecken ◽

Ute Stier

Keyword(s):

Transcriptional Regulation ◽

Retinoic Acid ◽

Carbonic Anhydrase ◽

Tumor Cell ◽

Cell Line ◽

Tumor Cell Line ◽

Carbonic Anhydrase Ii

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Pro-Angiogenic Effects of Resveratrol in Brain Endothelial Cells: Nitric Oxide-Mediated Regulation of Vascular Endothelial Growth Factor and Metalloproteinases

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/jcbfm.2012.2 ◽

2012 ◽

Vol 32 (5) ◽

pp. 884-895 ◽

Cited By ~ 43

Author(s):

Fabricio Simão ◽

Aline S Pagnussat ◽

Ji Hae Seo ◽

Deepti Navaratna ◽

Wendy Leung ◽

...

Keyword(s):

Nitric Oxide ◽

Vascular Endothelial Growth Factor ◽

Endothelial Cells ◽

Growth Factor ◽

Signaling Pathways ◽

Tube Formation ◽

Mitogen Activated Protein Kinase ◽

Vascular Endothelial ◽

Cerebral Endothelial Cells ◽

Endothelial Growth Factor

Resveratrol may be a powerful way of protecting the brain against a wide variety of stress and injury. Recently, it has been proposed that resveratrol not only reduces brain injury but also promotes recovery after stroke. But the underlying mechanisms are unclear. Here, we tested the hypothesis that resveratrol promotes angiogenesis in cerebral endothelial cells and dissected the signaling pathways involved. Treatment of cerebral endothelial cells with resveratrol promoted proliferation, migration, and tube formation in Matrigel assays. Consistent with these pro-angiogenic responses, resveratrol altered endothelial morphology resulting in cytoskeletal rearrangements of β-catenin and VE-cadherin. These effects of resveratrol were accompanied by activation of phosphoinositide 3 kinase (PI3-K)/Akt and Mitogen-Activated Protein Kinase (MAPK)/ERK signaling pathways that led to endothelial nitric oxide synthase upregulation and increased nitric oxide (NO) levels. Subsequently, elevated NO signaling increased vascular endothelial growth factor and matrix metalloproteinase levels. Sequential blockade of these signaling steps prevented resveratrol-induced angiogenesis in cerebral endothelial cells. These findings provide a mechanistic basis for the potential use of resveratrol as a candidate therapy to promote angiogenesis and neurovascular recovery after stroke.

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CO2 laser enhances the chilling tolerance of wheat seedlings by stimulating NO synthesis

Canadian Journal of Plant Science ◽

10.1139/cjps-2015-0385 ◽

2016 ◽

Vol 96 (5) ◽

pp. 796-807

Author(s):

Yi-ping Chen ◽

Qiang Liu ◽

Dong Chen

Keyword(s):

Nitric Oxide ◽

Laser Irradiation ◽

Sodium Tungstate ◽

Chilling Stress ◽

Chilling Tolerance ◽

The Other ◽

Control Group ◽

Wheat Seedlings ◽

Oxide Synthase ◽

Protein Treatment

To investigate the mechanism by which laser irradiation enhances the chilling tolerance of wheat seedlings, seeds were exposed to different treatments, and biochemical parameters were measured. Compared with the control group, chilling stress (CS) led to an increase in the concentrations of malondialdehyde (MDA) and H2O2, and decreases in the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), catalase (CAT), peroxidase (POD), and nitric oxide synthase (NOS), and the concentrations of nitric oxide (NO) and protein. Treatment with 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), sodium tungstate (ST), and NG-nitro-L-arginine methyl ester (L-NAME) followed by CS resulted in further increases in the concentrations of MDA and H2O2 and further decreases in the other parameters. However, treatment with PTIO, ST, and L-NAME followed by laser irradiation had the opposite effects on these parameters. When the seeds were treated with PTIO, ST, and L-NAME followed by laser and CS, the concentrations of MDA and H2O2 were significantly lower and the other parameters were higher than in the PTIO, ST, and L-NAME plus CS groups. These results suggest that CO2 laser irradiation enhances the chilling tolerance of wheat seedlings by stimulating endogenous NO synthesis.

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