scholarly journals Vitamin C supplementation reduces expression of circulating miR-451a in subjects with poorly controlled type 2 diabetes mellitus and high oxidative stress

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10776
Author(s):  
Laongthip Ruknarong ◽  
Chongchira Boonthongkaew ◽  
Nisa Chuangchot ◽  
Amonrat Jumnainsong ◽  
Naruemon Leelayuwat ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Vitamin C ◽  
Mirna Expression ◽  
Essential Element ◽  
Oxidative Status ◽  
Vitamin C Supplementation

Background Vitamin C is an essential element required for normal metabolic function. We investigated the effect of vitamin C supplementation on circulating miRNA (miR) expression in subjects with poorly controlled type 2 diabetes mellitus (T2DM). Changes in miR expression were also correlated with clinical measures of disease. Methods Pre- and post-vitamin C supplementation samples from five participants who had increased vitamin C levels, improved oxidative status and polymorphonuclear (PMN) function after receiving 1,000 mg of vitamin C daily for six weeks were screened for miRNA expression using the NanoString miRNA assay. Differences in miRNA expression identified from the miRNA screen were validated by qRT-PCR. Results Four miRNAs showed significantly different expression post-vitamin C supplementation relative to baseline, including the down-regulation of miR-451a (−1.72 fold change (FC), p = 0.036) and up-regulation of miR-1253 (0.62 FC, p = 0.027), miR-1290 (0.53 FC, p = 0.036) and miR-644a (0.5 FC, p = 0.042). The validation study showed only miR-451a expression was significantly different from baseline with vitamin C supplementation. MiR-451a expression was negatively correlated with vitamin C levels (r =  − 0.497, p = 0.049) but positively correlated with levels of malondialdehyde (MDA) (r = 0.584, p = 0.017), cholesterol (r = 0.564, p = 0.022) and low-density lipoproteins (LDL) (r = 0.522, p = 0.037). Bioinformatics analysis of the putative target genes of miR-451a indicated gene functions related to signaling pathways involved in cellular processes, such as the mammalian target of rapamycin (mTOR) signaling pathway. Conclusions Vitamin C supplementation altered circulating miR-451a expression. The results from this pilot study suggest that miRNAs could be used as biomarkers to indicate oxidative status in subjects with T2DM and with poor glycemic control and could lead to a novel molecular strategy to reduce oxidative stress in T2DM.

scholarly journals Type 2 Diabetes Mellitus And Vitamin C

2019 ◽  
Vol 09 (03) ◽  
pp. 232-236
Author(s):  
Shabzain Ishrat ◽  
Talea Hoor ◽  
Mohammed Sajid Abbas Jaffri
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Vitamin C ◽  
Good Control ◽  
Type 2 Dm ◽  
Vitamin C Supplementation ◽  
Antioxidant Supplements

Type 2 diabetes mellitus (DM) is a chronic disease which deteriorates the quality of life with time. Type 2 DM accounts for more than 90% cases of diabetes mellitus as compared to other types of this disease. There is significant oxidative stress in type 2 DM which plays an important role in the pathogenesis of disease. In order to combat this oxidative stress antioxidant supplements have to be added as add on therapy along with treatment of type 2 DM. Vitamin C is the safest antioxidant which plays significant role in diminishing the oxidative stress. The vitamin C supplementation have good control of FBS and HbA1c and therefore helps in achieving better glycemic control along with prevention of lipid abnormalities.

scholarly journals Study of role of vitamin C in type 2 diabetes mellitus patients

2021 ◽  
Vol 11 (1) ◽  
pp. 58
Author(s):  
Shashikala Eda ◽  
Somnath Motgi ◽  
Rohith Singh ◽  
Vijay Raghawa Rao B. N.
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Vitamin C ◽  
Glycosylated Hemoglobin ◽  
Public Health Problem ◽  
Hypoglycemic Agents ◽  
Global Public Health ◽  
Vitamin C Supplementation

Background: Diabetes mellitus (DM) is the most common non-communicable diseases in the present millennium which has become a global public health problem. The treatment of type 2 Diabetes mellitus (T2 DM) often is initiated with monotherapy of oral antidiabetic drugs (OADs), which often do not decrease the plasma sugar levels effectively and consistently that will reduce short term and long-term complications associated with T2 DM. Hence the current study is aimed to determine the effectiveness of vitamin C supplementation with standard OADs on glycemic control.Methods: This study consisted of 120 T2 DM patients with 80 males and 40 females with a mean age of 50.88 yrs were divided into four groups with equal number of males and females in each group depending upon the OADs they received in solo or with vitamin C for 12 weeks. After the written consent, a detail clinical history, clinical examination, biochemical investigations including fasting plasma sugar (FPS), post prandial plasma sugar (PPS), glycosylated hemoglobin (HBA1c), serum creatinine, serum electrolytes, chest X-ray PA view and standard ECG were done. Repeat FPS, PPS and HBA1c were done after 4, 8 and 12 weeks of study.Results: After 12 weeks of study FBS, PPS and HBA1c decreased significantly (p<0.01) in study groups (Metformin and teneligliptin with vitamin C) as compared to control groups (OADs without vitamin C). Vitamin C supplementation with OADs found to be effective, well tolerated and devoid of any side effects.Conclusions: OADs are effective and affordable hypoglycemic agents with vitamin C supplementation.

scholarly journals Assessment of oxidative damage and lipid profile levels in tobacco chewers with Type 2 diabetes mellitus

2018 ◽  
Vol 18 (1) ◽  
pp. 78-82
Author(s):  
T Nagamma ◽  
Nirjala Laxmi Madhikarmi ◽  
Singh PP
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Vitamin C ◽  
Oxidative Damage ◽  
Lipid Profile ◽  
Newly Diagnosed ◽  
Tobacco Chewing

Objectives: To assess oxidative damage and lipid profile levels in Type 2 diabetes mellitus patients with or without tobacco chewing habit. Methods: Total of 141 newly diagnosed Type 2 diabetes mellitus patients with tobacco chewing habit from past >18 years were included, 136 Type 2diabetes mellitus without any tobacco chewing habit, 140 normal healthy subjects without any tobacco chewing habit were included in the study. Blood sample was collected after 8-12 hours fasting from each subject to estimate glucose and cholesterol. The plasma was used for estimation of total antioxidant (TAA) activity, Vitamin C and thiobarbituric acid reacting substances (TBARS) by standard methods. Statistical analysis was done with SPSS version 16. Results: The glucose, cholesterol and TBARS levels were found to be increased significantly (p<0.001) in diabetic patients with tobacco chewing habit, as well as TAA, Vitamin C levels decreased significantly (p<0.001) when compared with healthy individuals. Conclusion: We observed increased oxidative stress and declined levels of antioxidants in newly diagnosed type 2 diabetes patients with tobacco chewing habit. It is known that hyperglycemia induces oxidative stress and further facilitate the progression of diabetic complications. Bangladesh Journal of Medical Science Vol.18(1) 2019 p.78-82

scholarly journals Study on Glycemic Statusand Oxidative Stress Level in Patients of Type 2 Diabetes Mellitus With and Without Retinopathy: A Teaching Hospital Based Study

2020 ◽  
Vol 9 (1) ◽  
pp. OT01-OT04
Author(s):  
Rachana Gahlawat ◽  
Dinesh Narain Saksena
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diabetic Retinopathy ◽  
Vitamin C ◽  
Antioxidant Status ◽  
Newly Diagnosed ◽  
Outpatient Services

Background: Diabetes is a chronic disease and sustained hyperglycemia attacks both micro vessels and macro vessels throughout the body. It is the leading cause of retinopathy, nephropathy, end-stage renal disease, non traumatic lower extremity amputations.Subjects and Methods:Twenty five (25) Patients with newly diagnosed type 2 diabetes mellitus without any signs of retinopathy were identified from outpatient services of department of Medicine and Twenty five (25) Patients with newly diagnosed type 2 diabetes mellitus with retinopathy were identified from outpatient services of department of Ophthalmology along with 25 healthy subjects were taken as controls. Diabetic retinopathy was diagnosed after doing detailed dilated fundoscopic examination as per the standard criteria.Results:The levels of FBS, HbA1c, total cholesterol, triglycerides and MDA were significantly higher in the diabetics without retinopathy, while the HDL and Vitamin C levels were lower as compared to those of the control group. The levels of FBS HbA1c, cholesterol, triglycerides and MDA were significantly higher in the diabetics with retinopathy, while the HDL and Vitamin C levels were lower as compared to those in the diabetes without retinopathy.Conclusion: The increased oxidative stress and a decreased antioxidant status can predict the micro-vascular complications in diabetes mellitus. The raised MDA levels indicate the oxidative stress and the decreased Vitamin C levels indicate the reduced antioxidant status in diabetic retinopathy.

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