scholarly journals Comparative transcriptome analysis of R3a and Avr3a-mediated defense responses in transgenic tomato

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11965
Author(s):  
Dongqi Xue ◽  
Han Liu ◽  
Dong Wang ◽  
Yanna Gao ◽  
Zhiqi Jia
Keyword(s):  
Late Blight ◽  
Transcriptome Analysis ◽  
Late Blight Resistance ◽  
Defense Responses ◽  
Endogenous Hormones ◽  
Comparative Transcriptome ◽  
Incompatible Interaction ◽  
Comparative Transcriptome Analysis ◽  
Phenylpropanoid Biosynthesis ◽  
Plant Pathogen Interaction

Late blight caused by Phytophthora infestans is one of the most devastating diseases in potatoes and tomatoes. At present, several late blight resistance genes have been mapped and cloned. To better understand the transcriptome changes during the incompatible interaction process between R3a and Avr3a, in this study, after spraying DEX, the leaves of MM-R3a-Avr3a and MM-Avr3a transgenic plants at different time points were used for comparative transcriptome analysis. A total of 7,324 repeated DEGs were detected in MM-R3a-Avr3a plants at 2-h and 6-h, and 729 genes were differentially expressed at 6-h compared with 2-h. Only 1,319 repeated DEGs were found in MM-Avr3a at 2-h and 6-h, of which 330 genes have the same expression pattern. Based on GO, KEGG and WCGNA analysis of DEGs, the phenylpropanoid biosynthesis, plant-pathogen interaction, and plant hormone signal transduction pathways were significantly up-regulated. Parts of the down-regulated DEGs were enriched in carbon metabolism and the photosynthesis process. Among these DEGs, most of the transcription factors, such as WRKY, MYB, and NAC, related to disease resistance or endogenous hormones SA and ET pathways, as well as PR, CML, SGT1 gene were also significantly induced. Our results provide transcriptome-wide insights into R3a and Avr3a-mediated incompatibility interaction.

scholarly journals Comparative transcriptome analysis reveals defense responses against soft rot in Chinese cabbage

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Mengyang Liu ◽  
Fang Wu ◽  
Shan Wang ◽  
Yin Lu ◽  
Xueping Chen ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Transcriptome Analysis ◽  
Soft Rot ◽  
Defense Responses ◽  
Comparative Transcriptome ◽  
Comparative Transcriptome Analysis

scholarly journals Transcript Dynamics in Wounded and Inoculated Scots Pine

2021 ◽  
Vol 22 (4) ◽  
pp. 1505
Author(s):  
Vilnis Šķipars ◽  
Dainis Ruņģis
Keyword(s):  
Scots Pine ◽  
Transcriptome Analysis ◽  
Protein Gene ◽  
Heterobasidion Annosum ◽  
Comparative Transcriptome ◽  
Cell Wall Modification ◽  
Comparative Transcriptome Analysis ◽  
Family Protein ◽  
Plant Pathogen Interaction ◽  
One Year

Comparative transcriptome analysis provides a useful tool for the exploration of plant–pathogen interaction by allowing in-depth comparison of gene expression between unaffected, inoculated and wounded organisms. Here we present the results of comparative transcriptome analysis in genetically identical one-year-old Scots pine ramets after wounding and inoculation with Heterobasidion annosum. We identified 230 genes that were more than 2-fold upregulated in inoculated samples (compared to controls) and 116 downregulated genes. Comparison of inoculated samp les with wounded samples identified 32 differentially expressed genes (30 were upregulated after inoculation). Several of the genes upregulated after inoculation are involved in protection from oxidative stress, while genes involved in photosynthesis, water transport and drought stress tolerance were downregulated. An NRT3 family protein was the most upregulated transcript in response to both inoculation and wounding, while a U-box domain-containing protein gene was the most upregulated gene comparing inoculation to wounding. The observed transcriptome dynamics suggest involvement of auxin, ethylene, jasmonate, gibberellin and reactive oxygen species pathways and cell wall modification regulation in response to H. annosum infection. The results are compared to methyl jasmonate induced transcriptome dynamics.

scholarly journals Comparative Transcriptome Analysis Reveals a Preformed Defense System in Apple Root of a Resistant Genotype of G.935 in the Absence of Pathogen

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Yanmin Zhu ◽  
Jonathan Shao ◽  
Zhe Zhou ◽  
Robert E. Davis
Keyword(s):  
Transcriptome Analysis ◽  
Genetic Regulation ◽  
Pythium Ultimum ◽  
Defense Responses ◽  
Comparative Transcriptome ◽  
Defense System ◽  
Apple Rootstock ◽  
Data Set ◽  
Resistant Genotype ◽  
Comparative Transcriptome Analysis

Two apple rootstock genotypes G.935 and B.9 were recently demonstrated to exhibit distinct resistance responses following infection by Pythium ultimum. As part of an effort to elucidate the genetic regulation of apple root resistance to soilborne pathogens, preinoculation transcriptome variations in roots of these two apple rootstock genotypes are hypothesized to contribute to the observed disease resistance phenotypes. Results from current comparative transcriptome analysis demonstrated elevated transcript abundance for many genes which function in a system-wide defense response in the root tissue of the resistant genotype of G.935 in comparison with susceptible B.9. Based on the functional annotation, these differentially expressed genes encode proteins that function in several tiers of defense responses, such as pattern recognition receptors for pathogen detection and subsequent signal transduction, defense hormone biosynthesis and signaling, transcription factors with known roles in defense activation, enzymes of secondary metabolism, and various classes of resistance proteins. The data set suggested a more poised status, which is ready to defend pathogen infection, in the root tissues of resistant genotype of G.935, compared to the susceptible B.9. The significance of preformed defense in the absence of a pathogen toward overall resistance phenotypes in apple root and the potential fitness cost due to the overactivated defense system were discussed.

Genome-wide comparative transcriptome analysis of the A4-CMS line ICPA 2043 and its maintainer ICPB 2043 during the floral bud development of pigeonpea

2021 ◽  
Author(s):  
Abhishek Bohra ◽  
Abhishek Rathore ◽  
Prasad Gandham ◽  
Rachit K. Saxena ◽  
S. J. Satheesh Naik ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Comparative Transcriptome ◽  
Bud Development ◽  
Comparative Transcriptome Analysis ◽  
Genome Wide ◽  
Cms Line ◽  
Floral Bud

Comparative transcriptome analysis reveals the key regulatory genes for higher alcohol formation by yeast at different α-amino nitrogen concentrations

2021 ◽  
Vol 95 ◽  
pp. 103713
Author(s):  
Ya-Ping Wang ◽  
Zhong-Guan Sun ◽  
Cui-Ying Zhang ◽  
Qiao-Zhen Zhang ◽  
Xue-Wu Guo ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Amino Nitrogen ◽  
Regulatory Genes ◽  
Comparative Transcriptome ◽  
Higher Alcohol ◽  
Comparative Transcriptome Analysis ◽  
Nitrogen Concentrations ◽  
Alcohol Formation
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