scholarly journals Construction and characterization of the bacteriophage P4 derivatives whose genome size suitable for packaging into a P2sir3-sized head

2015 ◽  
Vol 51 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Kyoung-Jin Kim
Keyword(s):  
Genome Size ◽  
Bacteriophage P4

Genome size and cytogenetic characterization of three Algerian Retama species

2011 ◽  
Vol 7 (5) ◽  
pp. 987-998 ◽  
Author(s):  
Radia Benmiloud-Mahieddine ◽  
Mona Abirached-Darmency ◽  
Spencer C. Brown ◽  
Meriem Kaid-Harche ◽  
Sonja Siljak-Yakovlev
Keyword(s):  
Genome Size ◽  
Cytogenetic Characterization

scholarly journals In Depth Characterization of Repetitive DNA in 23 Plant Genomes Reveals Sources of Genome Size Variation in the Legume Tribe Fabeae

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143424 ◽  
Author(s):  
Jiří Macas ◽  
Petr Novák ◽  
Jaume Pellicer ◽  
Jana Čížková ◽  
Andrea Koblížková ◽  
...  
Keyword(s):  
Genome Size ◽  
Repetitive Dna ◽  
Size Variation ◽  
Genome Size Variation ◽  
Plant Genomes

scholarly journals Genome Size Estimation of Callipogon relictus Semenov (Coleoptera: Cerambycidae), an Endangered Species and a Korea Natural Monument

Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 111
Author(s):  
Yun-Sang Yu ◽  
Soyeong Jin ◽  
Namjoon Cho ◽  
Jongok Lim ◽  
Cheol-Hak Kim ◽  
...  
Keyword(s):  
Endangered Species ◽  
Genome Size ◽  
Accurate Estimation ◽  
Size Estimation ◽  
Genome Size Evolution ◽  
Size Evolution ◽  
Longhorn Beetle ◽  
Natural Monument ◽  
First Time

We estimated the genome size of a relict longhorn beetle, Callipogon relictus Semenov (Cerambycidae: Prioninae)—the Korean natural monument no. 218 and a Class I endangered species—using a combination of flow cytometry and k-mer analysis. The two independent methods enabled accurate estimation of the genome size in Cerambycidae for the first time. The genome size of C. relictus was 1.8 ± 0.2 Gb, representing one of the largest cerambycid genomes studied to date. An accurate estimation of genome size of a critically endangered longhorned beetle is a major milestone in our understanding and characterization of the C. relictus genome. Ultimately, the findings provide useful insight into insect genomics and genome size evolution, particularly among beetles.

scholarly journals Characterization of bacteriophages used in the Salmonella enterica serovar Enteritidis phage-typing scheme

2009 ◽  
Vol 58 (1) ◽  
pp. 86-93 ◽  
Author(s):  
N. De Lappe ◽  
G. Doran ◽  
J. O'Connor ◽  
C. O'Hare ◽  
M. Cormican
Keyword(s):  
Genome Size ◽  
Salmonella Enterica ◽  
Pcr Amplification ◽  
Initial Step ◽  
Phage Typing ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Banding Patterns ◽  
Typing Scheme ◽  
Phage Dna

The 16 Salmonella enterica serovar Enteritidis (S. Enteritidis) typing phages (SETPs) used in the Laboratory of Enteric Pathogens (Health Protection Agency, London, UK) phage-typing scheme have not previously been characterized in detail. We have examined the adsorption properties of the phages with respect to a number of S. enterica serovars and defined phage morphology with electron microscopy. PFGE was used to estimate overall genome size and banding patterns generated by electrophoresis following restriction endonuclease digestion of the genome with HindIII were compared. PCR amplification and sequencing of selected genes was performed. The 16 phages comprise three morphotypes, Podoviridae (SETP1, 8, 10, 14, 15 and 16), Siphoviridae (SETP3, 5, 7, 11, 12 and 13) and Myoviridae (SETP2, 4, 6 and 9). All Podoviridae and Siphoviridae, but not Myoviridae, adsorbed to the O12 lipopolysaccharide antigen of Salmonella serogroups B (4,12) and D1 (9,12). The genome sizes for the Podoviridae and Siphoviridae (PFGE-A) were approximately 42 kb. The genome size for Myoviridae SETP2, 4 and 9 was 36.5 kb, and for myovirus SETP6 was 27 kb. HindIII digestion of phage DNA produced 9 distinct patterns of 8 to 11 bands. Relationships between phages based on digest patterns were consistent with those defined by morphology. The Podoviridae had homologues of several P22 genes while the Siphoviridae had homologues of several genes present in the sequenced siphovirus SETP3 (EF177456). This study represents an initial step in characterizing the molecular basis that underlies the widely used S. Enteritidis typing scheme.

scholarly journals Genomic characterization of Erethizon dorsatum papillomavirus 2, a new papillomavirus species marked by its exceptional genome size

2018 ◽  
Vol 99 (12) ◽  
pp. 1699-1704 ◽  
Author(s):  
Bert Vanmechelen ◽  
Roger K. Maes ◽  
Dodd G. Sledge ◽  
Samantha L. Lockwood ◽  
Stephanne L. Schwartz ◽  
...  
Keyword(s):  
Genome Size ◽  
Erethizon Dorsatum ◽  
Genomic Characterization

scholarly journals Partial Characterization of Bacteriophages from Indonesia and its Potency as Biocontrol of Xanthomonas oryzae pv. oryzae

2021 ◽  
Vol 25 (01) ◽  
pp. 75-80
Author(s):  
Desi Rejeki
Keyword(s):  
Biological Control ◽  
Genome Size ◽  
Crop Production ◽  
Xanthomonas Oryzae ◽  
Biological Control Agents ◽  
Vitro Assay ◽  
Resistant Varieties ◽  
A Genome

Bacterial leaf blight (BLB) is a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) of rice in rice-producing countries including Indonesia and attack rice in all stages of growth. In the advanced, crop production will be decreased by up to 50–70%. Recently, the practical efforts to overcome the problem by using resistant varieties, antibiotics, and sanitation; however, the ability of the pathogen to forms the new virulent pathotypes is noteworthy. Alternatively, the pathogen could be environmental-friendly controlled by utilizing bacteriophages as biological control agents because of their specific characteristics to their bacterial hosts. This research aimed to obtain information about the characteristic of the first isolated bacteriophages from Indonesia. The result showed that two bacteriophages had been isolated from soil in Arjasa Jember and soil in Gadingan Situbondo, namely phage XooX1IDN and phage XooX2IDN, respectively. The two phages were inactivated at 80ºC and stable at pH within the range of 6 to 8. The phage XooX1IDN has a genome size of approximately 39 kb, while phage XooX2IDN had a genome size 38 kb. Morphologically, both phages possessed the family of Myoviridae. Phage biocontrol in vitro assay showed that both phages significantly reduced the growth of BLB pathogen, indicating that both phages potentially, as biological control agents for BLB disease in rice. © 2021 Friends Science Publishers

Satellite bacteriophage P4: Characterization of mutants in two essential genes

Virology ◽  
1973 ◽  
Vol 53 (1) ◽  
pp. 24-39 ◽  
Author(s):  
Warren Gibbs ◽  
Richard Neal Goldstein ◽  
Roberta Wiener ◽  
Björn Lindqvist ◽  
Richard Calendar
Keyword(s):  
Essential Genes ◽  
Bacteriophage P4

Characterization of the small antisense CI RNA that regulates bacteriophage P4 immunity 1 1Edited by M. Gottesman

2002 ◽  
Vol 315 (4) ◽  
pp. 541-549 ◽  
Author(s):  
Francesca Forti ◽  
Ilaria Dragoni ◽  
Federica Briani ◽  
Gianni Dehò ◽  
Daniela Ghisotti
Keyword(s):  
Bacteriophage P4

scholarly journals Characterization of Rosa canina Fruits Collected in Urban Areas of Slovakia. Genome Size, iPBS Profiles and Antioxidant and Antimicrobial Activities

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1888 ◽  
Author(s):  
Katarína Rovná ◽  
Eva Ivanišová ◽  
Jana Žiarovská ◽  
Peter Ferus ◽  
Margarita Terentjeva ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Genome Size ◽  
Urban Areas ◽  
Antimicrobial Activities ◽  
Viable Count ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Rosa Canina ◽  
Interdisciplinary Study

The studies of plant bacterial endophytes, colonizing the plant tissues without any signs of diseases, are essential for understanding of ecological interactions. The aim of our study is to detect microbiological contamination and to assess the antimicrobial, antioxidant activity, total phenolic, carotenoid content, genome size, and ploidy of non-cultivated Rosa canina sampled from urban areas. Samples of Rosa canina fruits were collected in three locations in Slovakia. The highest total viable count and the Enterobacteriaceae count in fruits were 4.32 log CFU/g and 4.29 log CFU/g, respectively. Counts of the mesophilic anaerobic sporulating bacteria, Pseudomonas spp., and of the microscopic fungi and yeasts were 3.00, 2.15 log CFU/g, 3.65 log CFU/g, and 2.76 log CFU/g, respectively. Regarding the antimicrobial activity, Escherichia coli and Klebsiela oxytoca were the most sensitive species among the assayed microorganisms to the treatment with the ethanolic extracts of Rosa canina fruits. The fruits were rich in bioactive compounds, polyphenols, and carotenoids, that could be related to their antioxidant activity. Genome sizes of analyzed samples ranged from 2.3 to 2.96. DNA-based fingerprinting obtained by iPBS markers of the Rosa canina var. lapidicola Heinr. Braun., was characterized by some distinctive inserted loci. An interdisciplinary study was performed for the dog roses from different parts of Slovakia that resulted in deeper characterization of this species.

scholarly journals Identification and Genetic Diversity Analysis of Edible and Medicinal Malva Species Using Flow Cytometry and ISSR Molecular Markers

Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 650
Author(s):  
Iwona Jedrzejczyk ◽  
Monika Rewers
Keyword(s):  
Flow Cytometry ◽  
Molecular Markers ◽  
Genome Size ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Size Estimation ◽  
Flow Cytometric ◽  
First Choice ◽  
Issr Molecular Markers

The Malva genus contains species that reveal therapeutic properties and are mostly important in medicine and the functional food industry. Its breeding, cultivation, and utilization are based on proper germplasm/plant identification, which is difficult using morphological features. For this reason, we applied flow cytometry and inter simple sequence repeat polymerase chain reaction (ISSR-PCR) for fast and accurate species identification. Genome size estimation by flow cytometry was proposed as the first-choice method for quick accession screening. Out of the 12 tested accessions, it was possible to identify six genotypes based on genome size estimation, whereas all species and varieties were identified using ISSR markers. Flow cytometric analyses revealed that Malva species possessed very small (1.45–2.77 pg/2C), small (2.81–3.80 pg/2C), and intermediate (11.06 pg/2C) genomes, but the majority of accessions possessed very small genomes. Additionally, this is the first report on genome size assessment for eight of the accessions. The relationships between the investigated accessions showed the presence of two clusters representing malvoid and lavateroid group of species. Flow cytometry and ISSR molecular markers can be effectively used in the identification and genetic characterization of Malva species.

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