scholarly journals Characterization of Rosa canina Fruits Collected in Urban Areas of Slovakia. Genome Size, iPBS Profiles and Antioxidant and Antimicrobial Activities

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1888 ◽  
Author(s):  
Katarína Rovná ◽  
Eva Ivanišová ◽  
Jana Žiarovská ◽  
Peter Ferus ◽  
Margarita Terentjeva ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Genome Size ◽  
Urban Areas ◽  
Antimicrobial Activities ◽  
Viable Count ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Rosa Canina ◽  
Interdisciplinary Study

The studies of plant bacterial endophytes, colonizing the plant tissues without any signs of diseases, are essential for understanding of ecological interactions. The aim of our study is to detect microbiological contamination and to assess the antimicrobial, antioxidant activity, total phenolic, carotenoid content, genome size, and ploidy of non-cultivated Rosa canina sampled from urban areas. Samples of Rosa canina fruits were collected in three locations in Slovakia. The highest total viable count and the Enterobacteriaceae count in fruits were 4.32 log CFU/g and 4.29 log CFU/g, respectively. Counts of the mesophilic anaerobic sporulating bacteria, Pseudomonas spp., and of the microscopic fungi and yeasts were 3.00, 2.15 log CFU/g, 3.65 log CFU/g, and 2.76 log CFU/g, respectively. Regarding the antimicrobial activity, Escherichia coli and Klebsiela oxytoca were the most sensitive species among the assayed microorganisms to the treatment with the ethanolic extracts of Rosa canina fruits. The fruits were rich in bioactive compounds, polyphenols, and carotenoids, that could be related to their antioxidant activity. Genome sizes of analyzed samples ranged from 2.3 to 2.96. DNA-based fingerprinting obtained by iPBS markers of the Rosa canina var. lapidicola Heinr. Braun., was characterized by some distinctive inserted loci. An interdisciplinary study was performed for the dog roses from different parts of Slovakia that resulted in deeper characterization of this species.

scholarly journals Analysis of Antioxidant and Antiallergic Active Components Extracted From the Edible Insect Oxya yezoensis

2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110233
Author(s):  
Masahiro Saiki ◽  
Naomichi Takemoto ◽  
Maki Nagata ◽  
Masako Matsumoto ◽  
Yhiya Amen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Unsaturated Fatty Acids ◽  
Soluble Fraction ◽  
Total Phenolic ◽  
Key Factors ◽  
Active Components ◽  
Food And Agriculture ◽  
Food And Agriculture Organization

In recent years, entomophagy has attracted increased attention, as it was recommended as a potential source of food by the Food and Agriculture Organization of the United Nations. In Japan, Oxya yezoensisis one of the most widely eaten insect species, but studies of its functionality as a food are limited. In this study, we reported the optimal characterization of the total phenolic compounds in methanolic extract (OME) and different fractions of OME. Additionally, the antioxidant and antiallergic activities of the OME fractions were evaluated. The results showed that the ethyl acetate-soluble fraction of OME has potential antioxidant activity, whereas the n-hexane-soluble fraction showed the strongest inhibition of β-hexosaminidase, which is one of the key factors in allergic reactions. It was concluded that phenolic compounds might contribute to the antioxidant activity while unsaturated fatty acids contribute to the antiallergy activity.

DESIGN, SYNTHESIS, AND CHARACTERIZATION OF THE SOME NOVEL 2‐AMINO-PYRIDINE‐3‐ CARBONITRILE AND 2‐AMINO-4H-PYRAN‐3‐CARBONITRILE DERIVATIVES AGAINST ANTIMICROBIAL ACTIVITY AND ANTIOXIDANT ACTIVITY

2020 ◽  
pp. 125-134
Author(s):  
SURENDRA BABU LAGU ◽  
RAJENDRA PRASAD YEJELLA
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antimicrobial Activities ◽  
Docking Studies ◽  
Synthesis And Characterization ◽  
Zone Of Inhibition ◽  
Design Synthesis ◽  
Instrumental Methods ◽  
Objective Investigation

Objective: Investigation, the series of newer 2‐amino-pyridine‐3‐carbonitrile and 2‐amino-4H-pyran‐3‐carbonitrile derivative were synthesized and evaluated antimicrobial activities and antioxidant activity. Methods: Novel synthesized chalcones were further condensation to give 2-amino-3-cyanopyridine and 2-amino-3-cyanopyrans in the presence of malononitrile, pyridine, and ammonia acetate. The product is characterized by conventional and instrumental methods. Pyridine and 4-H-Pyran and their analogs occupy prime position due to their diverse applications. Results: The compounds A3C and B3C exhibited marked zone of inhibition with 30.02±0.02 mm and 29.06±0.01 mm, respectively. Docking studies suggested possible interactions with dihydrofolic reductase 4 with 9.15 and −9.67 kcal/mol, respectively. The IC50 30.28±0.01 exhibited A3C by 2,2-diphenylpicrylhydrazyl methods which is better among the series. The 2-amino-3-cyanopyridine derivatives were found good activity than 2-amino-3-cyanopyrane derivative. Among all synthesized compounds few having potent activity and some are near to the standard. Conclusion: Antimicrobial activity and antioxidant of the newly synthesized pyrans and pyridines derivatives will definitely inspire future researchers for the preparation of new analogs.

scholarly journals Enrichment of Phenolic Compounds from Olive Mill Wastewater and In Vitro Evaluation of Their Antimicrobial Activities

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Saleh Abu-Lafi ◽  
Mahmoud Sami Al-Natsheh ◽  
Reem Yaghmoor ◽  
Fuad Al-Rimawi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Olive Oil ◽  
Acid Value ◽  
Antimicrobial Activities ◽  
Olive Mill Wastewater ◽  
Total Phenolic ◽  
The Stability ◽  
Olive Mill

The production of olive oil generates massive quantities of by-product called olive mill wastewater (OMWW). The uncontrolled disposal of OMWW poses serious environmental problems. The OMWW effluent is rich in several polyphenolic compounds. Liquid-liquid extraction of OMWW using ethyl acetate solvent was used to enrich phenolic compounds under investigation. Total phenolic and flavonoid content and antioxidant activity of the extract were determined. HPLC coupled to photodiode array (PDA) detector was used to analyze the main three phenolic compounds of OMWW, namely, hydroxytyrosol, tyrosol, and oleuropein. The antimicrobial activity of the extract was also investigated. Additionally, the OMWW extract was used as natural preservative and antioxidants for olive oil. Results showed that OMWW is very rich in phenolic compounds and has strong antioxidant activity. HPLC analysis showed that the extract contains mainly hydroxytyrosol and tyrosol but no oleuropein. The OMWW extract showed also positive activities as antibacterial (gram positive and gram negative) and antifungal as well as activities against yeast. The addition of OMWW extract to olive oil samples has an effect on the stability of olive oil as reflected by its acid value, peroxide value, K232 and K270, and total phenolic content.

scholarly journals Antioxidant Activities, Total Phenolic Compound And Pigment Contents of Tropical Sargassum sp. Extract, Macerated In Different Solvents Polarity

2019 ◽  
Vol 22 (1) ◽  
pp. 73 ◽  
Author(s):  
Jelita Rahma Hidayati ◽  
Ervia Yudiati ◽  
Delianis Pringgenies ◽  
Zaenal Arifin ◽  
Diah Tri OktaviyantI
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compound ◽  
Antiradical Activity ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Phenolic Compound ◽  
Pigment Contents ◽  
Chlorophyll A Content

Exposure of sunlight lead tropical Sargassum sp. to maintain their growth and moreover to bring up their secondary metabollite for life struggling. Sargassum sp. has bioactive compounds that has a potential antioxidant acitivity such as phenolic compounds as well as chlorophyll and carotenoids. This research was conducted to determine antioxidant activities, phenolic compound and pigmens of Sargassum sp. with different solvent that have different polarities. Sample was macerate with n-heksana, ethyl acetate, methanol and aquadest. All the parameters were done spectrophotometrically. IC50 was used to determine the antioxidant activity by antiradical scavenging activity using DPPH (515 nm). Total phenolic compound were tested by Folin-Ciocalteu solution  and used gallic acid as standard (725 nm). The chlorophylls a content were measured at wavelength 662 nm and 645 nm and carotenoids were measured at wavelength 470 nm. The results showed best IC50 is achieved by aquadest extract (72.95 ±0.22 ppm). The highest Total phenolic compound is achieved by ethyl acetat extract (120.29 ±0,404 mg GAE/g sample). The highest chlorophyll a content is achieved by ethyl acetat extract (18.23 ± 0,049 mg/g sample) and the highest carotenoid content is achieved by ethyl acetat extract extract (60.65 ±0,008  µmol/g sample). It can be concluded that aquadest extract can be categorized as the strong antioxidant and antiradical activity, ethyl acetat as a medium antioxidant activity. The simple and save methods of aquadest extract promising that Sargassum  sp. frorn Indonesia is a good candidate compoud for nutraceutical and cosmeceutical approach.  

scholarly journals Chemical compositions, antioxidant and antimicrobial activities of Tubu (Pycnarrhena longifolia) leaves used as ingredient in traditional functional foods

Food Research ◽  
2020 ◽  
Vol 4 (3) ◽  
pp. 823-830
Author(s):  
N.K. Mohammed ◽  
B.J. Muhialdin ◽  
N.S. Masri ◽  
R. Sukor ◽  
F. Abd-El Aziem ◽  
...  
Keyword(s):  
Amino Acids ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Sugar Content ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Chemical Compositions ◽  
Food Ingredient

Tubu (Pycnarrhena longifolia) leaves are known as food enhancer and medicinal plant by several ethnics of Malaysia. The aims of the study were to determine the tubu leaves composition and biological activity including antioxidant and antibacterial. The mineral, sugar and amino acids were determined using HPLC, and bioactive compounds using GCMS. The antioxidant activity was evaluated by DPPH assay, and the antibacterial activity screened by disc diffusion method. The dry leaf compositions were 6.6% moisture, 11.6% ash, 7.0% crude protein, 15.7% crude fiber, 12.1% crude fat and carbohydrate 47%. A total of seven minerals were present in the methanol extract and the highest content was for iron (159.5 mg) and calcium (16.8 mg). The sugar profile showed high sugar content for glucose, sucrose, rhamnose, and fructose which was 8, 13, 7, and 20 mg/g, respectively. A total of sixteen amino acids was present in the tubu leaf extract, and seven bioactive compounds were identified by GC-MS/MS. The antioxidant activity was 87%, total phenolic compound content was 26.49 mg/mL and total flavonoid content was 13.39 mg/mL. Tubu leaves showed antibacterial activity towards Salmonella enterica serovar Typhimurium, Escherichia coli, Bacillus cereus and Staphylococcus aureus. This study showed that tubu leaves had rich sources of amino acids, minerals, sugars and bioactive compounds, potential to be a functional food ingredient.

Preliminary Phytochemical Screening, Evaluation of the Phenolic Compositions and Antioxidant Activities of Four Iranian Alyssum Species

2020 ◽  
Vol 16 (5) ◽  
pp. 581-587
Author(s):  
Pooran Golkar ◽  
Ahmad Fotoohi ◽  
Claudio Frezza
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Beta Carotene ◽  
Biochemical Properties ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Phytochemical Screening ◽  
Methanolic Extracts ◽  
Lobularia Maritima

Background: Alyssum L. is a genus of herbaceous perennial or annual plants belonging to the Brassicaceae family. Little is known about biochemical properties of Alyssum species, specially endemics to Iran. In particular, the species were A. homolocarpum (Fisch. & C.A.Mey.) Boiss., A. lepidotum Boiss., A. maritimum (L.) Lam. (now syn. of Lobularia maritima (L.) Desv.) and A. simplex Rudolph. and eight accessions were studied. These diverse species accessions were collected in six different areas. Materials and Methods: In this work, the Total Phenolic Content (TPC), the Total Flavonoid Content (TFD), the Total Flavonol Content (TFL), the Carotenoid content (Car) and the Anthocyanin content (Ant) of the leaf methanolic extracts of four Alyssum L. species collected in Iran, were assessed. The antioxidant activity assay for every extract obtained from the eight accessions was also carried out according to three distinct methodologies including three different methods including: DPPH, Beta carotene/ linoleic acid and phosphomolibdate assays. Results: The highest TPC, TFD and TFL values were separately observed in two different accessions of A. lepidotum whereas the highest carotenoid content was observed in one accession of A. homolocarpum and the highest anthocyanin content was observed in A. maritimum. Different results were observed for different methodolies for antioxidant evaluations methods and some of them were found to have values, expressed in mg/mL, much lower with respect to the control. The least DPPH activity and the highest total antioxidant activity with phosphomolybdate assay was found in A. lepidotum. Conclusion: The preliminary phytochemical screening and the evaluation of their antioxidant activities were reported here for the first time for Iranian Alyssum species. The findings eventually recommends, the use of those accessions in the ethnopharmacological and nutraceutical fields.

Deinbollia pinnata: Isolation and Characterization of Some Phytochemicals from Aerial Parts and their Antioxidant Potential

2020 ◽  
Vol 10 ◽  
Author(s):  
Yakubu Rufai ◽  
Norazah Basar ◽  
Aliyu Sani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Ferric Ion ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Isolation And Characterization ◽  
Ic50 Value

Background: Deinbollia pinnata belonging to Sapindaceae family are known as medicinal plants for the treatment of human diseases. Because of human over-exploitation, the choice of phytochemicals re-search and screening was basically made to add to the available compounds and ethno-medicinal records. Objective: To investigate phytochemicals and their antioxidant activities. Method: Antioxidant assays (DPPH, ABTS, FRAP) activities; phytochemical contents (Folin Ciocalteaus’s (FC) and aluminum chloride) methods; and phytochemical analysis were employed. Successful characterization of isolates using spectroscopic methods (1H NMR, 13C NMR, FTIR and GC-MS analysis). Results: Alkaloid, simple sugar, phenolics, tannins and steriods were present in all the parts. Saponin, terpenes and flavonoids were found in both leaves and root bark, while root bark and heartwood possessed quinones. The methanol extracts demonstrated total phenolic content at 75. 9 ± 2.05 GAE (mg GAE/g dw) and total flavonoid content at 88.41 ± 0.56 QUE (mg QUE/g dw). The plant extracts showed good DPPH scavenging activity with percentage inhibition more than 70% at 125 ppm. The methanol extracts showed excellent antioxidant activity towards ABTS assay with the IC50 value of 13.68 µg/mL. The extracts also exhibited the most potent ferric ion reducer at 3.45 ± 1.30 mM FRAP equivalent. Methyl gallate (2) displayed the highest antioxidant radical scavenging activity towards DPPH assay with the IC50 value of 0.19 µg/mL and pyrogallol (4) showed the highest ABTS activity with the SC50 value of 7.44 µg/mL. While, 3,4-dihydroxybenzoic acid (1) also showed the potential as ferric ion reducer ranging from 0.67± 0.01 to 3.63±0.12 mM FRAP equivalent respectively. Conclusion: Based on the antioxidant activity, methanolic extracts and its isolated constituents can be used as antioxidant sources for the management of oxidative causative diseases. Being the first report of these phytochemicals from genius Deinbollia and specie pinnata.

Ethnobotanical study, phytochemistry and antioxidant activity of medicinal plants used in the treatment of inflammation in two cities of Burkina Faso

2021 ◽  
Vol 2 (2) ◽  
pp. 01-012
Author(s):  
Armandine LEMA ◽  
Mindiédiba Jean BANGOU ◽  
Tangbadioa Hervé COULIDIATI ◽  
Mamadou SAWADOGO ◽  
Hyacinthe M THIOMBIANO ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Burkina Faso ◽  
Urban Areas ◽  
Polyphenolic Compounds ◽  
Total Phenolic ◽  
Ethnobotanical Study ◽  
Ethnobotanical Survey ◽  
Phytochemical Study ◽  
Traditional Practitioners

Background and Objective: The present study aimed to contribute to the knowledge of medicinal plants used in the treatment of inflammation in Burkina Faso, as well as to quanti fy the polyphenolic compounds content and to evaluate the antioxidant activity of two most solicited species. Materials and Methods: An ethnobotanical survey was carried out in the urban areas of Bobo-Dioulasso and Fada N'Gourma in order to collect sociodemographic and ethnobotanical data from traditional practitioners. Methanolic extracts of the plant organs were obtained using an extractor apparatus. The quantification of polyphenolic compounds contents was done by spectrophotometry, respectively with the Folin Ciocalteu reagent and aluminum trichloride. Likewise, antioxidant activity was evaluated by three methods (ABTS●+ DPPH● and FRAP) and the reading of optical densities was performed with the spectrophotometer. Results: One hundred and twelve (112) traditional practitioners were interviewed and seventy-three (73) plant species were identified in both areas. According to the frequency of quotation, Entada africana and Khaya senegalensis species were retained for further study. Phytochemical study revealed that the total phenolic content was high in the trunk bark of K. senegalensis (73.12 ± 1.8 mg GAE/100mg) and the total flavonoid content in the trunk bark of E. africana (8.42 ± 0.78 mg QE/100mg). Of the antioxidant activity, the highest value was observed at the level of the ABTS●+ radical inhibition method with the best result 13247.2 ± 219.84 µmol AAE/g (leaves of E. africana). Conclusion: The phytochemical analyses of these species indicate their richness in total phenolics and flavonoids contents, and could justify their use in the treatment of inflammatory diseases.

scholarly journals Composition, content of bioactive compounds, and antioxidant activity of fruit pulps from the Brazilian Amazon biome

2017 ◽  
Vol 52 (10) ◽  
pp. 933-941 ◽  
Author(s):  
Lara Borghi Virgolin ◽  
Fernanda Rosan Fortunato Seixas ◽  
Natália Soares Janzantti
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Brazilian Amazon ◽  
Soluble Solids ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Total Phenolic Compounds ◽  
Total Carotenoid Content

Abstract: The objective of this work was to evaluate the physicochemical composition, the bioactive compounds, and the total antioxidant activity of the fruit pulps of abiu (Pouteria caimito), achachairu (Garcinia humilis), araza (Eugenia stipitata), bilimbi (Averrhoa bilimbi), and yellow mangosteen (Garcinia xanthochymus) from the Brazilian Amazon biome. Total soluble solid content, total and reducing sugar contents, titratable acidity contents, pH, ascorbic acid content, moisture, protein, lipid, ash, and caloric value were determined. The contents of total anthocyanins, yellow flavonoids, and total carotenoids, besides total phenolic compounds and antioxidant activity, were also evaluated. The abiu pulp showed the highest contents of ash, total sugars, reducing sugars, total soluble solids, calorie value, and pH, among the analyzed fruits. The achachairu pulp showed the highest content of total phenolic compounds, with significant antioxidant activity, while the araza pulp had the highest total carotenoid content, and the yellow mangosteen, the highest yellow flavonoid content. The positive correlation between antioxidant activity and total phenolic compounds suggests that these compounds are the most important for determining antioxidant activity in the evaluated fruit pulps.

scholarly journals Effect of Natural Fermentation on the Chemical Composition, Mineral Content, Phytochemical Compounds, and Antioxidant Activity of Ziziphus spina-christi (L.) “Nabag” Seeds

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1228
Author(s):  
Amro B. Hassan ◽  
Salah A. Al Maiman ◽  
Mohammed A. Mohammed ◽  
Ghedeir M. Alshammari ◽  
Dalal A. Alkhudhayri ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Mineral Content ◽  
Crude Fiber ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Total Carotenoid ◽  
Total Carotenoid Content

Effects of fermentation on the chemical composition, mineral, total phenolic, total flavonoid, tannin, vitamin C, total carotenoid content, and antioxidant activity of “Nabag” Ziziphus spina-christi (L.) seeds were investigated. The fermentation process was carried out for 6, 12, 24, and 48 h. The fermentation significantly (p < 0.05) improved the chemical composition and mineral content of “Nabag” seeds, particularly the Ca, Fe, and Zn content. The phenolic, vitamin C, total carotenoid content, and antioxidant activity were significantly (p < 0.05) increased as a result of fermentation compared with unfermented Ziziphus spina-christi (L.) seeds. Fermentation of the seeds for 48 h resulted in the highest increase in crude fiber, Ca, Fe, Zn, and bioactive compounds. These results indicate the potential utilization of fermented “Nabag” seeds in the production and formulation of functional foods rich in crude fiber, essential minerals, and bioactive compounds.

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