scholarly journals Comparative Analysis of Immunohistochemistry of Hormone Receptors in Breast Cancer by Conventional and Microwave Tissue Processing Methods

2021 ◽  
Author(s):  
Janaky Ramakrishnan ◽  
Asiq N Sideeque ◽  
Reslin A Khader
Keyword(s):  
Predictive Value ◽  
Hormone Receptors ◽  
Tertiary Care ◽  
Progesterone Receptors ◽  
Microwave Processing ◽  
Kappa Statistics ◽  
Care Hospital ◽  
Cross Sectional ◽  
Early Therapy ◽  
Tissue Processing

Introduction: Rapid histopathological processing of neoplastic biopsies is crucial for initiating early therapy in patients. Microwave processing shortens the time to diagnosis and is less labour intensive. Aim: To compare the quality of immunohistochemical staining of Estrogen Receptors (ER) and Progesterone Receptors (PR) on paired breast samples using microwave processing versus conventional processing. Materials and Methods: A cross-sectional study was conducted at a tertiary care hospital in Kerala, India, from February 2014 to September 2015. A total of 44 paired samples from breast carcinoma patients were taken. Baseline characteristics of the patients such as age and type of carcinoma were noted. Haematoxylin and Eosin (H&E) stain was used. One slide of the pair was processed conventionally and the other using a microwave. The stained slides were observed by three pathologists. The validity of ER and PR receptor status was evaluated with sensitivity, specificity, Positive Predictive Value (PPV) and Negative Predictive Value (NPV). Kappa statistics and Spearman’s correlation test was used to denote the agreement between them. Results: The mean age was 51.32±11.16 years. There was agreement (Cohen k=0.902) between the microwave processed and conventionally processed paired breast samples for ER. The sensitivity and specificity of microwave processing in ER evaluation was 87.5% and 100% while that of PR evaluation was 92.9% and 100% respectively. The PPV was 100% and NPV was 93.3% for ER evaluation by microwave processing. Conclusion: Microwave aided tissue processing had significant advantages over conventional methods for providing a rapid diagnosis, being less laborious and had effective staining, which makes it a better choice.

Ultrasound Employed to Detect Breast Lumps among Symptomatic Patients in Tertiary Care Hospital

2020 ◽  
Vol 10 (4) ◽  
pp. 296-300
Author(s):  
Ameet Jesrani ◽  
Pari Gul ◽  
Nida Khan ◽  
Seema Nayab ◽  
Fahmida Naheed
Keyword(s):  
Predictive Value ◽  
Imaging Modality ◽  
Tertiary Care ◽  
Ultra Sound ◽  
Categorical Variables ◽  
Breast Lesions ◽  
Care Hospital ◽  
Continuous Variables ◽  
Cross Sectional ◽  
Breast Lumps

Objective: To assess different pathological breast lesions in ultra sound in a subgroup of population. Study design and setting: It was a cross sectional study conducted at Bolan Medical Complex Hospital Quetta, Pakistan from June 2018 to January 2019. Methodology: Total 103 patients with breast swelling, pain and discharge were targeted. Gray scale and Doppler Ultrasound of breast followed by FNAC/biopsy of breast lesion was performed. Data presented as mean ± standard deviation for continuous variables and frequency with percentages for categorical variables. Results: Out of 48 clinically palpable lumps US detected all of 48 lumps and additionally 12 clinically non palpable masses were detected on US examination. Thus, overall sensitivity of ultrasound in detecting breast lumps was 100%. Fibroadenoma of the breast was diagnosed accurately in 80.3% of women. Ultrasound reliably differentiated cystic from solid breast masses (100%). The sensitivity of ultrasound for detecting breast carcinoma was 63.4% with a positive predictive value of 87.5%, a negative predictive value of 99.5% and accuracy of 58.33%. US findings most suggestive of benign lesions were oval or round shape in 88.3%, well defined margin in 84%, absent lobulation in 86.04% and wider than taller ratio in 90.69% of the cases.US findings of most predictive for malignancy were of irregular shape in 81.8%, ill-defined margin in 90.9% and length to height ratio in 63.6% of cases. Conclusion: Ultrasound is simple, cheap, safe and relatively accessible imaging modality for evaluation of breast pathologies. Due to its high sensitivity in diagnosing benign breast lesions particularly cystic lesions and fibroadenoma unnecessary interventions can be avoided

scholarly journals Validity of Immunochromatographic Test for Antibody in Diagnosis of Typhoid Fever in Children Admitted in A Tertiary Care Hospital

2018 ◽  
Vol 16 (2) ◽  
pp. 20-24
Author(s):  
Zabeen Choudhury ◽  
Mohammed Rezaul Karim ◽  
Rasheda Samad ◽  
Shanjana Islam
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Predictive Value ◽  
Tertiary Care ◽  
False Negative ◽  
Care Hospital ◽  
Immunochromatographic Test ◽  
True Negative ◽  
College Hospital ◽  
Cross Sectional

Background: To determine the Validity of Immunochromatographic Test (ICT) in diagnosis of typhoid fever in children admitted in a tertiary care hospital.Methods: This cross sectional study was carried out the in Pediatric & Medicine wards of Chittagong Medical College Hospital (CMCH), Chittagong during the period July 2012 to June 2013. A total number of 150 clinically suspected cases of typhoid fever (Age >6 months to18 years) were enrolled in this study. After taking informed written consent, detailed history & clinical examination were completed. A blood culture sample was taken on the day of admission before starting antibiotic. On the 5th day onwards of appearance of fever, blood sample was taken to perform ICT. Patients received standard medical treatment of the admitting wards.Results: Blood C/S for Salmonella typhi was found positive in 16(10.7%) cases. Positive ICT for typhoid fever was found in 37(24.7%) cases. Among then, IgM was 18(12.0%) IgM+IgG were 8(5.3%) and IgG was 11(7.3%). ICT found true positive in 14, false positive in 23, false negative in 2 and true negative in 111 cases, where blood culture considered as gold standard. The difference was statistically significant (p<0.05) between two groups. Immunochromatographic Test (ICT) showed sensitivity 87.5%, specificity 82.8%, accuracy 83.3%, positive predictive value 37.8% and negative predictive value 98.2% for identification of typhoid fever.Conclusion: The present study has shown high sensitivity & specificity of ICT, it can be used as a useful & prospectful diagnostic tool.Chatt Maa Shi Hosp Med Coll J; Vol.16 (2); July 2017; Page 20-24

scholarly journals Role of CT enterography in evaluation of small bowel disorders

2019 ◽  
Vol 7 (2) ◽  
pp. 537
Author(s):  
Ritu N. Misra ◽  
Sunil Kr. Bajaj
Keyword(s):  
Small Bowel ◽  
Predictive Value ◽  
Imaging Modality ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Care Hospital ◽  
Ct Enterography ◽  
Cross Sectional ◽  
Bowel Diseases ◽  
Contrast Ct

Background: Small bowel pathologies are an enigma for clinicians and difficult to assess and evaluate for clinicians. In order to establish the efficacy of MDCT Enterography in diagnostic characterisation of small bowel lesions, the current study was undertaken.Methods: A prospective observational cross-sectional study was carried out in a tertiary care hospital. 30 patients with clinically suspected small bowel disease underwent CT enterography using iso-osmotic mannitol as neutral enteral contrast. CT enterography diagnoses were compared with clinical, surgical and histopathological results.Results: CT enterography showed a sensitivity (95.83%), specificity (100%), positive predictive value (100%), negative predictive value (85.71%), accuracy (96.66%) in diagnosis of small bowel diseases.Conclusions: CTE is a non-invasive well tolerated and reliable imaging modality for the depiction of small-bowel diseases. It provides excellent visualization of luminal, mural and extraintestinal findings.

scholarly journals Diagnostic and Prognostic Significance of CA 19-9 as Tumour Marker in Pancreatic, Hepatobiliary and Other Gastrointestinal Cancers

2021 ◽  
Author(s):  
Mitsu Vinay Vaishnav ◽  
Sameep Shyamlal Garg ◽  
Mayur Jitubhai Kokani
Keyword(s):  
Pancreatic Carcinoma ◽  
Predictive Value ◽  
Tertiary Care ◽  
Prognostic Significance ◽  
Tumour Marker ◽  
Enzyme Linked Immunosorbent Assay ◽  
Care Hospital ◽  
Prognostic Information ◽  
Cross Sectional

Introduction: The CA 19-9 antigen isolated by Koprowski and colleagues in 1979 is a lacto-N-fucopentaose II-like substance and one of the tumour-associated antigens present in serum in the mucin fraction. Close attention has been paid to the role CA 19-9 in the diagnosis of digestive tract tumours. In this study, serum analysis of CA 19-9 levels in 91 patients with gastrointestinal, hepatobiliary and pancreatic carcinoma was done. These data was used to evaluate the clinicians with adequate information on use of CA 19-9 as tumour marker- both diagnostic and prognostic. Aim: To study the role of tumour marker, CA 19-9 as a diagnostic and prognostic tool, and also to monitor the response of gastrointestinal, hepatobiliary and pancreatic cancer to treatment. Materials and Methods: This cross-sectional study was done on 91 cases of gastrointestinal, hepatobiliary and pancreatic carcinomas conducted in tertiary care hospital associated with medical college in Jamnagar, Gujarat, India were studied from September 2012 to March 2015 for two years and five months. The sample size was of 91 patients. Statistical method used was sensitivity, specificity, positive predictive value and negative predictive value. The material used was serum of the patient both pre as well as postoperatively and CalBiotech CA 19-9 Elisa Kit was used to determine the Value. The collected data were entered into Microsoft Excel spread sheet. The statistical methods used for variables were Mean and median along with Sensitivity and Specificity. Software used was “Epi Info”, version 7.0. Results: Total 91 cases of gastrointestinal, hepatobiliary and pancreatic carcinomas were studied. Enzyme Linked Immunosorbent Assay (ELISA) was used preoperatively and post operatively to determine the CA 19-9 values in patients of gastrointestinal, hepatobiliary and pancreatic carcinomas. It was found that CA 19-9 is an important tumour marker with sensitivity of 76.31% and specificity of 73.33% for diagnosis of the gastrointestinal, hepatobiliary and pancreatic carcinoma. When aided with Fine Needle Aspiration Cytology (FNAC) and histopathological findings it helps in giving a sure shot diagnosis. It also provides useful prognostic information for the same. Conclusion: This study helps to understand the role of CA 19-9 as diagnostic and prognostic marker for pancreatic, hepatobiliary and gastrointestinal carcinomas.

scholarly journals Evaluation Of Phenotypic Methods For Detection Of Carbapenem Resistance In Isolates Of Pseudomonas Aeruginosa In A Tertiary Care Hospital

2019 ◽  
Vol 09 (03) ◽  
pp. 165-169
Author(s):  
Shaista Bakhat ◽  
Yasmeen Taj ◽  
Faisal Hanif ◽  
Muhammad Faisal Faheem
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Predictive Value ◽  
Research Study ◽  
Tertiary Care ◽  
Carbapenem Resistance ◽  
Cross Sectional Study ◽  
Routine Practice ◽  
Care Hospital ◽  
Cross Sectional ◽  
Phenotypic Methods

Objective: To assess the effectiveness of Modified Hodge test (MHT) and Modified carbapenemase inactivation method (mCIM) for detection of carbapenemase enzyme produced by Pseudomonas aeruginosa strains that code for resistance towards carbapenem. Study Design and Setting: This observational cross sectional study was carried out from January 18 to January 19 in microbiology department of PNS SHIFA Karachi. Methodology: 140 isolates of Pseudomonas aeruginosa were cultured from pus samples of hospitalized patients from different wards like ENT, Surgery, Burn Unit, Plastic Surgery, ICU, Medicine, Pediatrics and family ward. These isolates were tested carbapenem resistance by two phenotypic methods namely MHT and mCIM test. This data was analyzed by using SPSS Version 23.0. Results: In our research study mCIM method showed 100% sensitivity, 99.1% specificity, positive predictive value 96.1% and negative predictive value 100% as compare to Modified Hodge Test which gave 77% sensitivity, 99.1% specificity, 95.2% PPV and 100% NPV. Conclusion: mCIM test is simple, accurate and more reliable method for detection of carbapenemase production as compared with MHT. It is recommended, cabapenemase producing isolates should be tested as a routine practice by all clinical labs laboratory.

scholarly journals Study of ER PR receptors in breast carcinoma: a study of 30 cases

2018 ◽  
Vol 5 (11) ◽  
pp. 3738
Author(s):  
Himanshu R. Patel ◽  
Ankur Shrimal ◽  
Harsh P. Trivedi
Keyword(s):  
Breast Cancer ◽  
Retrospective Study ◽  
Breast Carcinoma ◽  
Hormone Receptors ◽  
Tertiary Care ◽  
Progesterone Receptors ◽  
Care Hospital ◽  
Breast Cancer Patients ◽  
Estrogen And Progesterone Receptors ◽  
Medical College

Background: Estrogen and Progesterone receptors are found in breast cancer cells that depend on estrogen and related hormones to grow. These are steroidal nuclear type of receptors. The most common method currently used to test a tumor for estrogen and progesterone receptors is immunohistochemistry or IHC. Patients with positive hormone receptor cancer have better survival. They are candidates of anti-hormonal therapy.Methods: This is a retrospective study was carried out in the Department of Surgery of a tertiary care hospital, affiliated to Government Medical College. This is a retrospective study in which 30 randomly selected case records of breast cancer patients, who presented in the period between 2011 to 2016, and who had undergone surgery, were taken and studied in terms of history, examination, investigations, treatment given, histopathology report and development of recurrence, metastasis and survival.Results: In this study out of 30 patients, 25 patients received adjuvant chemotherapy. 2 patients developed recurrence at local site.1 patient was ER/PR both negative and 1 patient was ER +/PR-. 3 patients developed metastasis. Among these, 2 patients were ER/PR both negative and 1 patient was having ER/PR both positive.Conclusions: Hormone receptors play a significant role in breast carcinoma. Breast cancer is more common in postmenopausal group as compared to premenopausal group. Mean age at diagnosis of breast cancer in western countries is 61 years and in India it is 50 years.

scholarly journals Is Ultrasound a Viable Imaging Modality for the Diagnosis of Hepatocellular Carcinoma? A Cross-Sectional Study

2021 ◽  
Vol 15 (1) ◽  
pp. 9-11
Author(s):  
Reeta Rani ◽  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Predictive Value ◽  
Tertiary Care ◽  
False Negative ◽  
Cross Sectional Study ◽  
Arterial Phase ◽  
Care Hospital ◽  
Sectional Study ◽  
Mri Scan ◽  
Cross Sectional

Background: Radiologists are always questioning the use of an imagine modality as compared to the other, this study helps answer that question for the diagnosis of Hepatocellular carcinoma. Objective: To compare the use of MRI imaging technique with ultrasonography for the diagnosis of hepatocellular carcinoma. Study Design: The type of study is a Prospective Cross-Sectional study. Settings: A large tertiary care hospital in Karachi, Pakistan. Duration: One year from July 2018 to July 2019. Methodology: The inclusion criteria was all the patients who were referred to us with symptoms for concern for hepatic tumors. For the MRI we used a 1.5 tesla machine, and for the ultrasound we used a conventional grey scale ultrasonography with a probe of 5 MHz. We performed multiphasic contrast enhanced MRI’s, with images taken both before the injection of the contrast and afterwards in the various phases such as the arterial phase, portal venous phase and finally the delayed phase respectively. Results: The study population consisted of n=110 patients having a mean age of 46.5 +/- 5.50 years. There were n= 78 (70.90%) males and n= 32 (20.09%) females. N= 101 (91.81%) patients had a diagnosis of hepatocellular carcinoma as per MRI scan, for ultrasound n= 47 (42.72%) patients were diagnosed as true positive, n= 2 (1.81%) were false positive, n= 54 (49.09%) were false negative, while n= 7 (6.36%) cases were truly negative. The specificity was 77.77% the sensitivity was 46.53%, the positive predictive value was 95.91%, negative predictive value was 11.4%, and accuracy was 49.09% respectively. Conclusion: We found that the specificity of ultrasound as a diagnostic modality for hepatocellular carcinoma as compared to the MRI scan is good, however it is only able to correctly identify about half the patients, hence care should be taken when interpreting the results of ultrasound for hepatocellular carcinoma.

scholarly journals Study of the role of hysteroscopy with dilation and curettage in post- menopausal bleeding: a cross sectional study

2020 ◽  
Vol 9 (5) ◽  
pp. 1954
Author(s):  
Atul Padmawar ◽  
Ankita Khande
Keyword(s):  
Predictive Value ◽  
Tertiary Care ◽  
Abnormal Uterine Bleeding ◽  
Cross Sectional Study ◽  
Uterine Bleeding ◽  
Care Hospital ◽  
Cross Sectional ◽  
Dilation And Curettage ◽  
Post Menopausal

Background: Abnormal uterine bleeding is defined as any type of bleeding in which the duration, frequency or amount is excessive for an individual patient. Hysteroscopy has ushered a new era in the evaluation of abnormal uterine bleeding. With this background, we conducted a study to find the role of hysteroscopy with dilation and curettage in post-menopausal bleeding.Methods: It was an observational study conducted in the department of obstetrics and gynecology of a tertiary care hospital in Maharashtra. Post-menopausal patients with abnormal uterine bleeding, admitted in the period of March 2018 to September 2019 were included in the study. A pretested and predesigned questionnaire was used to collect the data. Hysteroscopy with dilation and curettage was done and samples were sent for histopathology.Results: Bleeding was present in all the 30 cases. About 33.33% had pain abdomen, 56.67% had hot flushes, 10.00% had mass and 10% had other symptoms in the present study. The sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy of hysteroscopy in diagnosing malignancy was 75%, 96.15%, 75%, 96.15% and 93.33% respectively.Conclusions: To conclude the most common cause of abnormal uterine bleeding in post-menopausal women in our study was polyp. The sensitivity and specificity of the hysteroscopy in diagnosing the malignant lesions is 75% and 96.15% respectively which corroborated with other study findings. Hysteroscopy with dilation and curettage is better alternative to hysterectomy and biopsy in most of the cases. 

scholarly journals Effectiveness Of Urine Dipstick In Diagnosis Of Pre-eclampsia

2019 ◽  
Vol 10 (1) ◽  
pp. 3-6
Author(s):  
Ferhat Uzair ◽  
Urooj Naz ◽  
Samia Shuja ◽  
Abdul Waheed ◽  
Tahira Assad
Keyword(s):  
Diagnostic Accuracy ◽  
Pregnant Women ◽  
Predictive Value ◽  
Tertiary Care ◽  
First Trimester ◽  
P Value ◽  
Care Hospital ◽  
Singleton Pregnancy ◽  
Cross Sectional ◽  
Urine Dipstick

Objective: To determine the efficacy of urine dipstick in Pre-eclamplasia. Study Design and Setting: This was a cross-sectional study conducted at the department of obstetrics & gynecology of a tertiary care hospital from May-2017 to September-2018. Methods: All the pregnant women of age 15-45 years, BP=140/90 mmHg on two occasions 4 hours apart or single diastolic value of 100 mmHg, gestational age >20 weeks, singleton pregnancy, booked in first trimester of pregnancy were included in the study. Statistical package SPSS 22 was used for the analysis of data chi-square tests were applied for the diagnostic accuracy of urine dipstick in detection of Pre-eclampsia in pregnant women. P-value was <0.05 was considered as significant. Results: Total 211 pregnant women were enrolled in the study. Mean (SD) age was 28.26(3.86) years. Overall sensitivity and specificity of urine dipstick was found to be 94% and 95.4% respectively, positive predictive value 95%, negative predictive value 94.5% and excellent diagnostic accuracy 94.7% of urine dipstick was observed for the diagnosis of spot urine protein creatinine ratio CONCLUSION: Urine dipstick found to be sensitive and reliable test to screen pre-eclampsia

Confidence toward vaccination as reported by parents of children admitted to a tertiary care hospital in Riyadh, Saudi Arabia: A cross sectional study

Vacunas ◽  
2020 ◽  
Vol 21 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Y.M. AlGoraini ◽  
N.N. AlDujayn ◽  
M.A. AlRasheed ◽  
Y.E. Bashawri ◽  
S.S. Alsubaie ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Care Hospital ◽  
Sectional Study ◽  
Cross Sectional
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