Î±-GLUCOSIDASE INHIBITOR AND ANTIOXIDANT ACTIVITY ASSAYS OF GUAVA LEAF, CASHEW LEAF AND THE COMBINATIONS AS ANTIDIABETIC AGENT

Background: Previous identification of N,N-diethylthiobarbiturates as potential α-glucosidase inhibitory potential prompted us to investigate the antiglycation activity of these synthetic compounds (1-25) in order to identify the lead candidates for their possible antidiabetic potential. Methods: Synthetic compounds (1-25) were evaluated for their antiglycation activity using Bovine Serum Albumin assay (BSA). Results: Compounds exhibited varying degree of inhibition in the range of IC50 = 61.16 ± 2.3 - 656.71 ± 2.5 µM as compared to the standard rutin (IC50 = 294.5 ± 1.50 µM). Among the twenty five synthetic molecules, seven compounds showed good activity in comparison with the standard. Compound 4 (IC50 = 61.16 ± 2.3 µM) having hydroxy substituents was the most active molecule of the library. This study revealed that compound 4 has dual acting antidibetic molecule. Conclusion: In conclusion, the synthetic N,N-diethylthiobarbiturates can act as lead molecules. Furthermore, synthetic variations on N,N-diethylthiobarbituric acid moiety might be helpful in generating a library of potential anti diabetic agent. Especially, compound 4 has been identified as dual acting antidiabetic agent i.e. α-glucosidase inhibitor and antiglycating agent.

Download Full-text

Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract

Food Chemistry ◽

10.1016/j.foodchem.2006.07.034 ◽

2007 ◽

Vol 103 (2) ◽

pp. 381-388 ◽

Cited By ~ 170

Author(s):

Suganya Tachakittirungrod ◽

Siriporn Okonogi ◽

Sombat Chowwanapoonpohn

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

Antioxidant Action ◽

Guava Leaf

Download Full-text

A facile approach synthesis of benzoylaryl benzimidazole as potential α-amylase and α-glucosidase inhibitor with antioxidant activity

Bioorganic Chemistry ◽

10.1016/j.bioorg.2021.105073 ◽

2021 ◽

pp. 105073

Author(s):

Lotfi M. Aroua ◽

Hind R. Almuhaylan ◽

Fahad M. Alminderej ◽

Sabri Messaoudi ◽

Sridevi Chigurupati ◽

...

Keyword(s):

Antioxidant Activity ◽

Glucosidase Inhibitor

Download Full-text

Bioactive constituents from the leaves of Quercus phillyraeoides A. Gray for α-glucosidase inhibitor activity with concurrent antioxidant activity

Food Science and Human Wellness ◽

10.1016/j.fshw.2016.02.004 ◽

2016 ◽

Vol 5 (2) ◽

pp. 85-94 ◽

Cited By ~ 3

Author(s):

Anastasia Wheni Indrianingsih ◽

Sanro Tachibana

Keyword(s):

Antioxidant Activity ◽

Bioactive Constituents ◽

Inhibitor Activity ◽

Glucosidase Inhibitor ◽

Quercus Phillyraeoides

Download Full-text

Antioxidant Activity Study And Α-glucosidase Inhibitor Phenolic Leaf Extract Bungur (Lagerstroemia speciosa) From Meru National Park Betiri

Jurnal ILMU DASAR ◽

10.19184/jid.v17i1.2672 ◽

2017 ◽

Vol 17 (1) ◽

pp. 39 ◽

Cited By ~ 1

Author(s):

Jainur Rochman ◽

Tri Agus Siswoyo ◽

A.A. Istri Ratnadewi

Keyword(s):

Antioxidant Activity ◽

Superoxide Anion ◽

National Park ◽

Digestive System ◽

Methanol Extract ◽

Ethyl Acetate Extract ◽

Total Phenolic ◽

Dpph Radical ◽

Glucosidase Inhibitor ◽

Phenolic Extract

Study activities phenolic extract of bungur leaf as an antioxidant and inhibitor for the enzyme α-glucosidase was performed by spectrophotometric method. The extraction was carried out by three solvents with different polarity level in order to get three types of extracts namely hexane extract Bungur (HB), ethyl acetate extract Bungur (EAB), and the methanol extract Bungur (MB). The units, used in the analysis, is the total phenolic that standardized in gallic acid. The antioxidant activity of phenolic extracts Bungur leaf can be seen from its ability to reduce free radicals through the damping test DPPH radical, superoxide anion, and hydroxyl. Potential extract phenolic leaf Bungur as an inhibitor of α-glucosidase were analyzed by inhibition of α-glucosidase, but in this study also tested the inhibition of the enzyme α-amylase because both of these enzymes worked on the digestive system and hydrolyze carbohydrates. The results generally showed that the methanol extract of leaves Bungur potential as antioxidants that compared with standard vitamin C. In addition, it had as well as potential for inhibitors of α-glucosidase and α-amylase that compared akarbosa standard, which is expected to be a natural antidiabetic agent.Keywords: phenolic, bungur, antioxidant, inhibitor, α-amilase, α-glukosidase

Download Full-text

Efficient Synthesis of 2-[(3,4-Dichlorophenyl)methyl]-1H-benzimidazole Derivatives, and their α-Glucosidase Inhibitory and Antioxidant Activities

Letters in Organic Chemistry ◽

10.2174/1570178617666200330130024 ◽

2020 ◽

Vol 17 (10) ◽

pp. 806-814

Author(s):

Emre Menteşe ◽

Fatih Yılmaz ◽

Nimet Baltaş

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Benzimidazole Derivatives ◽

Efficient Synthesis ◽

Antioxidant Power ◽

Glucosidase Inhibitor ◽

Ferric Reducing Antioxidant Power ◽

Inhibitory Activities

In this work, some new 2-[(3,4-dichlorophenyl)methyl]-1H-benzimidazole derivatives containing different five-membered heterocycles like 1,3,4-oxadiazole, 1,3,4-triazole, and 1,3,4- thiadiazole moieties were designed and efficiently synthesized starting from 2-[(3,4-dichlorophenyl) methyl]-1H-benzimidazole. The products were screened for their in vitro antioxidant and α-glucosidase inhibitory activities. Among the synthesized compounds, some of them showed efficient α-glucosidase inhibition with IC50 values ranging between 16.05 ± 0.94 and 77.02 ± 1.12 μg/mL when they were compared with the standard α-glucosidase inhibitor acarbose having IC50 value 12.04 ± 0.68 μg/mL. The antioxidant activity of all products was screened by using various in vitro antioxidant assays, including CUPric Reducing Antioxidant Capacity (CUPRAC) and Ferric Reducing Antioxidant Power (FRAP) assays. Also, the radical scavenging activities of the products were assayed by using the ABTS method. method.

Download Full-text

ANTI-DIABESITY PRINCIPLE FROM THE SEEDS OF PHYLLANTHUS EMBLICA L.

INDIAN DRUGS ◽

10.53879/id.57.12.12323 ◽

2021 ◽

Vol 57 (12) ◽

pp. 41-50

Author(s):

Priyanka Rathod ◽

Chandana Kulkarni ◽

Raman P. Yadav

Keyword(s):

Antioxidant Activity ◽

Pancreatic Lipase ◽

Inhibitory Activity ◽

Methanolic Extract ◽

Scavenging Activity ◽

Phyllanthus Emblica ◽

Natural Substrate ◽

Glucosidase Inhibitor ◽

Pancreatic Lipase Inhibition ◽

Lipase Inhibition

In recent years, pancreatic lipase inhibitor and α- glucosidase inhibitor have been highlighted as potential anti-diabesity principles. In the present study, seeds of Phyllanthus emblica L. (Family: Phyllanthaceae) was studied for anti-diabesity potential in terms of pancreatic lipase inhibitory activity, α-glucosidase inhibitory activity and antioxidant activity. At 100μg/ml concentration, pancreatic lipase inhibition of the methanolic extract using synthetic substrate obtained was 73.2±0.1% (IC50 59.1μg/ml), whereas pancreatic lipase inhibition using natural substrate was 87.9 ± 2.62%. α- glucosidase inhibition of the extract at 50μg/ml was measured as 94.4±0.37% (IC50 34.4μg/ml). The superoxide scavenging activity of the extract was found to be 81.5±0.41%. Interestingly, upon TLC fingerprinting, only one band with Rf 0.70 showed multifunctional activity. The phytochemical found to be present was an alkaloid. The results evidenced the presence of multifunctional smart molecule in methanolic extract of P. emblica L and showed an alkaloid as the component responsible for anti-diabesity potential.

Download Full-text