scholarly journals Determination of Genetic Diversity of Four Guppy Strains Using the Random Amplified Polymorphic DNA-PCR

2021 ◽  
pp. 1-8
Author(s):  
Riva Hafidah ◽  
Ayi Yustiati ◽  
Yuniar Mulyani ◽  
Ibnu Bangkit Bioshina Suryadi
Keyword(s):  
Genetic Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Descriptive Analysis ◽  
Similarity Index ◽  
Quantitative Descriptive Analysis ◽  
Rapd Pcr ◽  
Result Show ◽  
Pcr Method ◽  
Quantitative Descriptive

This research aims to determine genetic diversity of four strains guppy, respectively are japan blue double sword (JBD), japan blue tiger double sword (JBTD), blue moscow (BM), and panda guppy (PG) with RAPD-PCR method. The obtained genetic diversity data is used as guide reference for hybridization between four strains. The research was conducted in September 2020 to April 2021 with explorative methods and in qualitative and quantitative descriptive analysis. The research was carried out in biotechnology Laboratory, Fishery and Marine Sciences Faculty and Central Laboratory,Padjadjaran University, Indonesia.Strains of JBD, JBTD, BM obtained fromCilengkrangSubdistrict, Bandung and PG strain obtained from Parung market, Bogor. Primary OPA-03 (AGTCAGCCAC) is used for standard parameters to interpret genetic diversity among four strains of guppy. Based on results, amplification with OPA-03 primary visualize 25 bands that include five polymorphic bands and 20 monomorphic bands. The phylogenetic tree result show that there are two relationship groups. The first group are JBD, JBTD, and BM with similarity index in the range of 80-89%The first group consist two sub groups of relationship. The first sub group are JBD and JBTD with similarity index of 89%. The second sub group is BM with similarity index of 80%. The second group isPG with similarity index of 65.5%.

scholarly journals EFEKTIVITAS BALI GO LIVE SEBAGAI MEDIA PROMOSI DIGITAL PARIWISATA BALI

Jurnal IPTA ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 16
Author(s):  
Ni Luh Putu Septiani ◽  
I Nyoman Sudiarta ◽  
Putu Agus Wikanatha Sagita
Keyword(s):  
Sampling Method ◽  
Descriptive Analysis ◽  
Validity And Reliability ◽  
Epic Model ◽  
Quantitative Descriptive Analysis ◽  
Analysis Technique ◽  
Effectiveness Measurement ◽  
Quantitative Descriptive ◽  
Model Approach

The purpose of this research is to know the effectiveness of using Bali Go Live Channel as media promotion digital of Bali Tourism by using EPIC Model approach. Effectiveness measurement is done by obtaining value of empathy dimension, persuation dimension, impact dimension, and communication dimension from digital promotion conducted by Bali Go Live Channel to know how effective promotion is done. Determination of samples in this study using accidental sampling method as much as 100 respondents tourists. Data collection techniques used questionnaires and interviews. Data analysis technique in this research is quantitative descriptive analysis, validity and reliability analysis, simple tabulation analysis, and EPIC Model analysis. From the results of quantitative descriptive analysis can be seen that the measurement of dimensions empathy, dimension persuasion, dimension impact, and dimension communication entered in the range of effective scale. The results of this study show the use of Bali Go Live Channel as a digital promotion media of Bali tourism declared effective. This is shown from the EPIC Rate of 3.98 which fall into the effective category.

scholarly journals PENGARUH PENGUASAAN PSIKOLOGI PEMBELAJARAN TERHADAP PENINGKATAN KOMPETENSI GURU PENDIDIKAN AGAMA ISLAM

2017 ◽  
Vol 2 (02) ◽  
pp. 191-199
Author(s):  
Mustahidang Usman ◽  
Nurhidaya M
Keyword(s):  
Descriptive Analysis ◽  
Islamic Education ◽  
Sample Population ◽  
Analysis Method ◽  
Quantitative Descriptive Analysis ◽  
Effective Learning ◽  
Teacher Competency ◽  
Quantitative Descriptive ◽  
Method Approach

ABSTRAK Jenis penelitian adalah penelitian Survey dengan pendekatan metode kuantitatif dengan mengeksplorasi data dilapangan dengan metode analisis deskriktif kuantitatif. Mengingat bahwa populasi guru di sekolah ini kurang dari 100 orang guru maka penetapan sampel pada penelitian ini adalah sampel populasi atau keseluruhan dari populasi yaitu 18 guru.  Hasil penelitian ini menunjukkan bahwa Penguasaan psikologi pembelajaran terhadap peningkatan kompetensi guru Pendidikan Agama Islam di SMA Muhammadiyah Sungguminasa Kabupaten Gowa yaitu Bahwa penguasaan psikologi pembelajaran, mulai dari memahami siswa sebagai pelajar, memilih metode yang sesuai keadaan peserta didik, membantu peserta didik yang kesulitan dalam belajar dan lain sebagainya , sangat dituntut untuk dimiliki oleh seorang guru, agar seorang guru dapat menciptakan suasana pembelajaran yang kondusif dan efektif. Tingkat kompetensi guru Pendidikan Agama Islam di SMA Muhammadiyah Sungguminasa Kabupaten Gowa yaitu sudah baik, terbukti dari pemahaman guru tersebut tentang 4 rana kompetensi ( paedagogik, sosial, kepribadian, dan profesional) yang cukup luas dan penilaian dari rekan-rekan guru tentang kompetensi yang dimilikinya yang mencapai 97,2 % atau 16 orang guru menjawab baik. Kata Kunci : Psikologi  Pembelajaran Kompetensi Guru  ABSTRACT The type of research was survey research with quantitative method approach by exploring field data with quantitative descriptive analysis method. Given that the teacher population in this school was less than 100 teachers then the determination of the sample in this study was the sample population or the entire population of 18 teachers. The results of this study indicate that the mastery of learning psychology on improving the competence of teachers of Islamic Education in SMA Muhammadiyah Sungguminasa Gowa District that was mastery of learning psychology, ranging from understanding students as students, choosing methods appropriate to the state of learners, helping learners who have difficulty in learning and etc., was required to be owned by a teacher, so that a teacher can create a conducive and effective learning atmosphere. The level of competence of teachers of Islamic Education in SMA Muhammadiyah Sungguminasa Gowa District was good, proven from the teacher's understanding of the 4 broadest range of competence (paedagogic, social, personality, and professional), and the assessment from the teachers about their competence reached 97.2% or 16 teachers answered well. Keywords: Psychology of Teacher Competency Learning

scholarly journals Analisis Konsentrasi Spasial dan Strategi Pengembangan Industri Kecil di Jawa Timur

2017 ◽  
Vol 4 (1) ◽  
pp. 58
Author(s):  
Nurul Ma'rifatus Sa'diyah ◽  
Siti Komariyah ◽  
Anifatul Hanim
Keyword(s):  
Analytical Hierarchy Process ◽  
Descriptive Analysis ◽  
Quantitative Descriptive Analysis ◽  
Strategy Of Development ◽  
Small Industry ◽  
Quantitative Descriptive ◽  
Small Industries ◽  
Hierarchy Process

This study aims to determine the location of the concentration of industry in East Java and determines the development strategyof small industry in East Java. In this study using quantitative descriptive analysis tools and Analytical Hierarchy Process(AHP). Based on the results of this research is that the location of concentration of small industries in East Java, contained infour district/city namely: Tulungagung, Malang, Blitar and Bondowoso. In the determination of strategy of development ofsmall industries in order of priority to consider the overall criteria (training, financing, partnerships, technology) weconcluded the first priority of increased promotion, differentiation of products, improving the quality of human resources andthe expansion of the market.

scholarly journals Antibacterial Effects of Octenidine Dihydrochloride, and Benzalkanium Chloride on Acinetobacter Baumannii Strains Isolated from Clinical Samples and Determination of Genetic Diversity of Isolates by RAPD-PCR Method

2021 ◽  
Author(s):  
Azar Dokht Khosravi ◽  
Effat Abasi Montazeri ◽  
Seyedeh Roya Maki
Keyword(s):  
Genetic Diversity ◽  
Acinetobacter Baumannii ◽  
Clinical Samples ◽  
Biochemical Tests ◽  
Typing Method ◽  
Conventional Culture ◽  
Rapd Pcr ◽  
Octenidine Dihydrochloride ◽  
Pcr Method

Abstract Due to the emergence of antibiotic resistance in Acinetobacter baumannii which is one of the important causes of nosocomial infections, many problems have been raised in the successful treatment of patients with the subsequent mortality. So, the present study was performed to evaluate the antibacterial effect of Actinidine dehydrochloride, Actinisept, and Benzalkanium chloride against Acinetobacter baumannii strains isolated from clinical samples and to determine the genetic diversity of strains by RAPD-PCR. A total of 119 non-duplicate, suspected Acinetobacter baumannii isolates were collected and confirmed by conventional culture and biochemical tests and PCR technique. Susceptibility of the isolates to antibiotics was evaluated by standard Antibiotic susceptibility testing (AST). For antiseptics Octenidine dihydrochloride (OCT), Actinisept, and Benzalkonium chloride (BZK), Minimal inhibitory concentration (MIC) was assessed. The prevalence of Qac E and Qac delta E genes related to antiseptics was estimated by PCR. Finally, genetic diversity of strains was determined by RAPD-PCR. All 119 suspected isolates were confirmed as Acinetobacter baumannii using conventional tests and PCR. The isolates were mostly originated from blood samples. In AST, the lowest resistance was seen for ciprofloxacin and gentamicin. The MIC values were reported as OCT (15.26 µg) and BZK (640 µg). The antiseptic genes of qacE and qac ΔE1 were found to be present in 56 (47.05%) and 59 (49.57%) of isolates respectively. RAPD typing method revealed great diversity among A. baumannii isolates, with 37 clusters in isolates from ICU, of which 32 isolates were single and 5 were multiple. In conclusion, considering the increase of resistance to antiseptics, it is of importance to monitor the susceptibility of A. baumannii to antiseptics and to promote antiseptic stewardship in hospitals. Furthermore, in this study great diversity among A. baumannii was observed making it difficult to properly carry out infection control policies. analysis of RAPD-PCR typing results, and we found 37 clusters, among them 32 isolates were single and 5 were multiple. So, the method generated 37 RAPD type which shows great diversity among 57 out of 62 A. baumannii isolates at 80% cutoff.

scholarly journals Genetic structure and diversity among three Greek sheep breeds using Random Amplified Polymorphic DNA-PCR

2017 ◽  
Vol 62 (4) ◽  
pp. 301 ◽  
Author(s):  
I. MASTRANESTASIS (Ι. ΜΑΣΤΡΑΝΕΣΤΑΣΗΣ) ◽  
Ch. LIGDA (Χρ. ΛΙΓΔΑ) ◽  
K. THEODOROU (Κ. ΘΕΟΔΩΡΟΥ) ◽  
L. V. EKATERINIADOU (Λ.Β. ΑΙΚΑΤΕΡΙΝΙΑΔΟΥ)
Keyword(s):  
Genetic Structure ◽  
Genetic Differentiation ◽  
Phylogenetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Genetic Identity ◽  
Rapd Pcr ◽  
Pcr Method ◽  
Genetic Structure And Diversity

Genetic structure and diversity of 120 animals from three Greek local breeds were investigated by Random Amplified Polymorphic DNA (RAPD) - PCR method. Sheep samples originated from the Lesvos, Chios and Karagouniko breeds were treated with 11 random primers to estimate their genetic diversity and phylogenetic relationships. Our analysis comprised two levels of the breeds' genetic structure: i) the genetic differentiation among the three breeds and ii) the genetic differentiation among the flocks within each breed. This combined approach gave two main findings: i) the study of genetic distances and identity revealed that the Karagouniko sheep breed is genetically distinct from Chios (GD=0.1979) and Lesvos (GD=0.1691) breeds, while ii) the Chios and Lesvos breeds are genetically similar (GI=0.9631); half of the flocks of Lesvos have a relatively closer relationship with those of Chios than with the other Lesvos flocks. This is the first study that reports the close genetic relationship between the Chios and Lesvos breeds and gives strong evidence to hypotheses about their related origin. Furthermore, the study of polymorphic loci revealed particular indicators located in Karagouniko breed, as definitional datum of genetic identity or as a fingerprint of breed. Therefore, RAPD-DNA methods can be an efficient tool for the determination of phylogenetic relationships and genetic identity among the bloodstock breed of sheep.

scholarly journals Perhitungan harga pokok produksi berdasarkan metode Full Costing untuk penetapan harag jual produk pada CV. Silvi MN Paradilla Parengan

Owner ◽  
2022 ◽  
Vol 6 (1) ◽  
pp. 632-647
Author(s):  
Laras Sukma Arum Melati ◽  
Guntur Saputra ◽  
Faridatun Najiyah ◽  
Fitria Asas
Keyword(s):  
Descriptive Analysis ◽  
Production Costs ◽  
Selling Price ◽  
Quantitative Descriptive Analysis ◽  
Basic Product ◽  
Cost Of Production ◽  
Quantitative Descriptive ◽  
The Cost

The problem that becomes the background of this research is in determining the selling price of a basic product that is used is the calculation of the cost of production, which is a way to take into account the determination of cost elements into the cost of the product and the selling price that is set must be able to determine all costs that produce long term profit. Based on the determination of the correct product cost of a product, it will be able to reduce uncertainty in determining the selling price. The purpose of this study is to find out how to calculate the cost of production based on the full costing method for determining the selling price of the product. In determining the selling price of the product, the selling price method is used based on cost-plus pricing. Cost plus pricing is the determination of the price by adding a certain amount (percentage) of the selling price or cost as profit. The method used in this study is quantitative descriptive analysis method, the results of this study indicate that there are advantages in calculating the cost of goods manufactured based on the Full Costing method and to be able to determine the cost of goods sold, the production costs must be calculated at the beginning of each month based on the previous period's sales report.

scholarly journals SPK Pemberian Pinjaman dalam Upaya Mengantisipasi Kredit Macet pada Anggota Koperasi Studi Kasus: KSP Warna Artha

2020 ◽  
Vol 20 (2) ◽  
pp. 369
Author(s):  
Imron Imron
Keyword(s):  
High Risk ◽  
Descriptive Analysis ◽  
Demand Analysis ◽  
Savings And Loans ◽  
Quantitative Descriptive Analysis ◽  
Interview Method ◽  
Loan Demand ◽  
Quantitative Descriptive ◽  
Main Activity

Cooperative Savings and Loans is one type of cooperative in Indonesia that has the main activity is to provide storage services and loan funds to members of the cooperative with the aim of advancing the welfare of members in particular and society in general. From that base, it can be seen that cooperatives contain two elements, namely economic elements and social elements related to each other. It is said to have an economic element because the purpose of the cooperative itself is to achieve the welfare of members. The loan facility in the Artha Color cooperative has a high risk of non-performing loans or bad loans. The incidence of further problem loans may result in difficulties from the cooperative. This study aims to analyze the procedure of granting small and medium business loans and employee loans. The subjects of this research are staff of Artha Color Cooperative employees and members of Artha Color Cooperative. The object of his research is the analysis of the determination of lending employees to members of the cooperative. The data collected by interview method, observation and documentation are then analyzed by Quantitative Descriptive Analysis. The results showed that lending procedures to members of the cooperative has been well implemented starting from the submission of loan demand, analysis of guarantee files, conducting surveys, studying loan files, to disbursement of credit.

scholarly journals QUANTITATIVE DESCRIPTIVE ANALYSIS METHODOLOGY FOR THE DETERMINATION OF THE OPTIMUM MONTH FOR CONSUMPTION OF DAYLILIES (HEMEROCALLIS SPP.)

HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 513E-514
Author(s):  
A.N. Pollard ◽  
P. Coggins ◽  
P.R. Knight ◽  
C.E. Coker
Keyword(s):  
Descriptive Analysis ◽  
Early Morning ◽  
Sensory Attributes ◽  
Quantitative Descriptive Analysis ◽  
Analysis Methodology ◽  
Significant Difference ◽  
Quantitative Descriptive ◽  
The Ancient Chinese ◽  
Descriptive Language

There are >52,000 cultivars of daylilies (Hemerocallis spp.), some of which the ancient Chinese used for nutritional and medicinal purposes. Daylilies have tremendous potential in foodservice due to the range of color, fragrance, flavor, and textural varieties. The objective was to quantify by descriptive analysis the attribute descriptors for two edible daylily cultivars; that is, `Rosie Meyer' and `Siloam Powder Pink,' and to provide data indicating the optimum month for consumption of these two cultivars of daylilies. Blooms were harvested during early morning hours over 5 months (May through September). Six panelists were trained to use Quantitative Descriptive Analysis for 6 months on the sensory attributes of `Rosie Meyer' and `Siloam Powder Pink' cultivars. Training consisted of determining attribute categories as well as intensity ratings for references. Attribute categories included appearance, odor/scent, handfelt texture, oral texture, basic taste(s), flavor, mouthfeel factors, and chew rate. Results showed attribute categories with descriptors for `Siloam Powder Pink' as odor/scent, flavor, mouthfeel factors, and oral texture as having a significant difference (P < 0.05) for the evaluation months. `Rosie Meyer' demonstrated significant differences (P < 0.05) for the evaluation months in attribute categories for appearance, odor/scent, flavor, oral texture, and mouthfeel factors. The optimum month for consumption of `Rosie Meyer] was September (P < 0.05); for `Siloam Powder Pink,' it was July (P < 0.05). These results provide a descriptive language for daylily cultivars `Rosie Meyer' and `Siloam Powder Pink.' The optimum month for consumption to obtain the fullest range of desirable attributes will provide the food and horticultural industries with added information by which to market these cultivars.

scholarly journals Genetic Relationship of the Transgenic and Non-Transgenic Mutiara Catfish with their Hybrids Sangkuriang Catfish

2019 ◽  
Vol 4 (2) ◽  
pp. 204-213
Author(s):  
Iskarimah Yolanisa ◽  
Ibnu Dwi Buwono ◽  
Yuniar Mulyani ◽  
Iskandar Iskandar
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Clarias Gariepinus ◽  
Similarity Index ◽  
Transgenic Fish ◽  
Fast Growth ◽  
Relationship Analysis ◽  
Rapd Pcr ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Relationship Of

The male broodstock of Transgenic Mutiara catfish (P) (containing Clarias gariepinus Growth Hormone, CgGH) is a fish who show fast growth. Propagation of the Transgenic Mutiara catfish F1 and F2 progeny involves the crossing of broodstock P with Non Transgenic Mutiara catfish. New genetic variations (polymorphisms) can be generated from crosses broodstock P and F1 which lead to the fast growth inheritance of transgenic fish. Phylogenetic relationship analysis showed distance and genetic variation in broodstock P, F1, F2 and Sangkuriang catfish (as controls) using the RAPD-PCR method (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) as the research purposes. The results showed that OPA-03 (5'-AGTCAGCCAC-3 ') primer was selective for determining phylogenetic among catfish samples. The closest genetic similarity index originates from the female broodstock of Non Transgenic Mutiara catfish (P) (93%), while the farthest originates from the male broodstock of Mutiara Transgenic catfish (P) (51%) with F1 dan F2. Closely related broodstock catfish is not recommended to be crossed.

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