Gc-Ms Analysis of Cordia dichotoma G. Forst Bark Hexane Extract and Evaluation of Anti Bacterial Activity

In this study, the antimicrobial compounds present in leaves of Alphonsea madraspatana was identified by using RP-HPLC, LC/MS and GC/MS and found to be active against multiple drug resistant (MDR) bacteria e.g. Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Extraction of dried plant leaves was performed by solvent gradient technique. Antimicrobial study was performed with each extract. n-Hexane and methanol extracts were further subjected to analysis for identification of potent antimicrobial compounds due to their better antimicrobial activity as compared to remaining extracts. The methanolic extract was more active than n-hexane extract. As n-hexane extract contains non-polar compounds, it was subjected to GC-MS analysis and methanol extract was subjected to RPHPLC followed by LC/MS due to presence of polar components. Among the isolated compounds, four compounds were identified as potent antimicrobial. RP-HPLC and LC/MS analysis of methanol extract also ensured the presence of four potent antimicrobial compounds as follows: (i) kaempferol-3-O-rotinoside-orhamnoside (m/z 748), (ii) 3-hydroxypropylglucosinolate (m/z 377), (iii) luteolin-7-O-glucoside (m/z 448) and (iv) genistein-7-O-glycoside (m/z 432). Leaves of this plant may be used as potent antibacterial agents due to presence of antimicrobial compounds.

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PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF BIOACTIVE CONSTITUENTS OF THE HEXANE EXTRACT FROM ACANTHOSPERMUM HISPIDUM (whole plant)

Journal of Chemical Society of Nigeria ◽

10.46602/jcsn.v46i1.575 ◽

2021 ◽

Vol 46 (1) ◽

Author(s):

Akoh, O. U ◽

Mac-Kalunta O. M ◽

Emewusim, C. M

Keyword(s):

Hexane Extract ◽

Raw Material ◽

Phytochemical Screening ◽

Bioactive Components ◽

Bioactive Constituents ◽

Invasive Weed ◽

Ms Analysis ◽

Standard Procedures ◽

Whole Plant ◽

Entire Plant

Although Acanthospermum hispidum has long been considered as a competitive invasive weed of agricultural plantations, it has recently become a sought after raw material to manufacture syrups in the treatment of asthma, bronchitis and fever. In the present study, the bioactive components of Acanthospermum hispidium entire plant have been accessed via GC-MS analysis. Six compounds from the hexane extract of the whole plant were identified. The most prevalent are glycerine (100.00%), 1-benzoypiperidine (40.26%) and erythritol (15.79%). Phytochemical screening investigation carried out using standard procedures showed the presence of Alkaloids, Steriods, Saponnins, Tannin, Flavonoids, Phenol, Terpenoids.

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GC-MS analysis of the essential oil, aroma components and n-hexane extract of St. John Wort (Hypericum perforatum L., Hypericaceae)

Macedonian Pharmaceutical Bulletin ◽

10.33320/maced.pharm.bull.2018.64.01.006 ◽

2018 ◽

Vol 64 (01) ◽

pp. 63-77

Author(s):

Arijeta Shabani ◽

Marija Karapandzova ◽

Ivana Cvetkovikj Karanfilova ◽

Gjose Stefkov ◽

Svetlana Kulevanova

Keyword(s):

Essential Oil ◽

Hypericum Perforatum ◽

Experimental Studies ◽

Hexane Extract ◽

Bioactive Constituents ◽

Specific Chemical ◽

Ms Analysis ◽

Hexane Extracts ◽

Pharmacological Potential ◽

Aroma Components

St. John Wort (Hypercum perforatum L., Hypericaceae) has been used as a medicinal plant for a long period of time as this plant is characterized by a diversity of bioactive constituents which possess well documented pharmacological activities including antiviral, antimicrobial, anti-inflammatory, antioxidant, hepatoprotective and anti-tumoral activity. Nowadays, special interest is put on its essential oil as some experimental studies showed great biological and pharmacological potential. According this, the main goal of this study was GC/MS analysis of the essential oil, aroma components as well as n-hexane extracts of Hypericum perforatum that grows in Western region in R. Macedonia. GC/FID/MS analyses of the isolated essential oils from leaf, flower and herb resulted in the identification of 84 compounds. The fraction of sesquiterpenes was dominated in all examined oils and the main constituents were germacrene D (17.77-39.03%), E-caryophyllene (11.37-25.71%) and β-selinene (0.69-4.77%). GC/HS/MS analyses of the aroma components resulted in the identification of 23 compounds. Among them, isononane was identified as main aroma component (up to 75%). GC/FID/MS analyses of the n-hexane extracts resulted in the identification of 60 compounds which were characterized by the presence of terpenoid (mono- and sesquiterpene) components and non-terpenoid constituents mainly consisted of hydrocarbons and their oxygenate derivatives and related components. The non-terpenoid fraction represented the largest part of the analysed extracts. The most abundant were nonacosane (15.45-49.28%), octacosane (1.33-40.05%) and pentacosane (1.68-9.04%). The aerial parts of H. perforatum collected from Western part of R. Macedonia could be considered as a good source of essential oil with specific chemical profile as well as aroma components and high lipophilic compounds, but further investigation should be done in accordance to their possible commercial or medicinal use. Keywords: Hypericum perforatum, essential oil, aroma components, n-hexane extract, GC-MS

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PHYTOCHEMICAL ANALYSIS AND CONSTITUENTS OF HEXANE EXTRACT OF MELASTOMA MALABATHRICUM L

Journal of Institute of Science and Technology ◽

10.3126/jist.v23i1.22150 ◽

2018 ◽

Vol 23 (1) ◽

pp. 18-25 ◽

Cited By ~ 1

Author(s):

Dil Prakash Giri ◽

Meena Rajbhandari

Keyword(s):

Free Radical ◽

Ethyl Acetate ◽

Methanol Extract ◽

Total Sugar ◽

Hexane Extract ◽

Total Phenolic ◽

Aqueous Methanol ◽

Methanol Extracts ◽

Melastoma Malabathricum ◽

Ms Analysis

Hexane, dichloromethane, ethyl acetate, methanol and 50 % aqueous methanol extracts of leaf, flower and fruit of Melastoma malabathricum were screened for the presence of different classes of phytochemicals. In phytochemical screening, ethyl acetate, methanol and 50 % aqueous methanol extracts showed the presence of phenolics and flavonoids. Glycosides were found only in the 50 % methanol extract of fruit. Free radical scavenging activity of methanol extract of leaf, flower and fruit was determined using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical. Total phenolic content was measured spectrophotometrically using Folin-Ciocalteu, total flavonoids content by using aluminum chloride and total sugar content by using anthrone reagent. Gallic acid was used as a standard for the calibration of phenolics, catechin for flavonoids and glucose for carbohydrates. The highest amount of phenolic was found in the 50 % methanol extract of leaf (242.88 ± 3.52 mg GAE/g extract), the highest amount of flavonoids was found in the ethyl acetate extract of leaf (127.41 ± 6.33 mg CE/g extract). Total sugar in 50 % methanol extract of fruit was found to be 151.12 ± 8.79 mg GE/g extract. Methanol extracts of flower showed the strongest antioxidant activity with IC50 value of 17.23 μg/ml. Silyl derivative of hexane extracts were prepared by using N-methyl-N-(trimetylsilyl) trifluoroacetamide for gas chromatography-mass spectroscopic (GC-MS) analysis. The GC-MS analysis of hexane extract allowed the identification of 17 phyto-constituents by comparing the fragmentation pattern of each component in mass spectrum with the standard NIST mass spectral database.

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