A Comparative Assessment on Antioxidant and Phytochemical of Trichilia monadelpha (Thonn) J.J. De Wilde (Meliaceae) Plant Extracts

This study was designed to evaluate the phytochemical screening and antioxidant activities of Trichilia monadelpha plant extracts. The leaves were extracted using two different solvents namely-(n-hexane and methanol). The dry or wet yield of n-hexane extract was 1.3%, while methanolic extract exhibited a percentage yield of 3.7 %. Phytochemical research revealed the presence of secondary plant metabolites such as; alkaloids, flavonoids, cardiac glycosides, terpenoids and saponins. In-vitro antioxidant activity was determined using three assays (DPPH free radical scavenging assay, reducing ability and hydroxy radical scavenging activity) with four concentrations (0.25, 5.0, 1.0 and 2.0 mg/L), vitamin C was also used as a standard antioxidant. The percentage of inhibition was measured, and the results from all assay models showed a concentration-dependent percentage of inhibition by the methanol extract. However, the percentage values for the 2,2, -diphenyl-1-picrylhydrazyl (DPPH), reducing ability and hydroxy radical scavenging activity assays of T. monadelpha leaf was lower than that of standard vitamin C. The Pearson’s correlation coefficient was evaluated and the results showed that of the two extracts, the methanolic extract had the most antioxidant activity with the methanol extract exhibiting a better significant correlation that had a similar trend to that of the antioxidant compound (vitamin C). The results of this study have shown that the plant Trichilia monodelpha contains bioactive compounds which may have contributed to its antioxidant properties.

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Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

Open Life Sciences ◽

10.2478/s11535-013-0247-0 ◽

2014 ◽

Vol 9 (2) ◽

pp. 149-155 ◽

Cited By ~ 4

Author(s):

Mirjana Marčetić ◽

Silvana Petrović ◽

Marina Milenković ◽

Marjan Niketić

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Chloroform Extract ◽

Volatile Fraction ◽

Scavenging Activity ◽

Antioxidant Power ◽

Aerial Parts ◽

Antimicrobial And Antioxidant Activity

AbstractThe chemical composition, antimicrobial and antioxidant activity of Eryngium palmatum, an endemic plant species from the Balkan Peninsula, were investigated. The flavonoids apigenin (9.5±0.3 mg g−1) and apigenin 7-O-glucoside (2.4±0.1 mg g−1) were determined in a methanol extract of aerial parts using HPLC analysis. The methanol extract of roots contained catechin (5.0±0.1 mg g−1), epicatechin (2.9±0.1 mg g−1), chlorogenic acid (1.6±0.0 mg g−1), gallic acid (0.9±0.0 mg g−1) and rosmarinic acid (0.9±0.2 mg g−1). GC-FID and GCMS analysis of a chloroform extract of aerial parts showed that the main volatile constituents were falcarinol, linoleic acid, hexadecanoic acid and methyl linoleate (comprising 32.6%; 24.4%; 19.9; 13.2% of the volatile fraction, respectively), while octanoic acid, tetradecanol and dodecanol dominated in the chloroform extract of the roots (34.9%; 25.8%; 22.2% of the volatile fraction, respectively). Investigation of antimicrobial activity by broth microdilution showed that the methanol and chloroform extracts of aerial parts and roots exerted a significant effect (MIC 3.5–15.6 μg mL−1) against tested Gram-positive and Gram-negative bacteria. The methanol extracts of aerial parts or roots exerted moderate ferric reducing antioxidant power, DPPH radical scavenging activity and hydroxyl radical scavenging activity.

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Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

Hemijska industrija ◽

10.2298/hemind160518047g ◽

2017 ◽

Vol 71 (5) ◽

pp. 361-370

Author(s):

Slavica Grujic ◽

Ana Dzamic ◽

Violeta Mitic ◽

Vesna Stankov-Jovanovic ◽

P.D. Marin ◽

...

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Radical Scavenger ◽

Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

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Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v8i12.2749-2755.4038 ◽

2020 ◽

Vol 8 (12) ◽

pp. 2749-2755

Author(s):

Mohammad Afzal Hossain ◽

Nadia Khan Disha ◽

Jahid Hasan Shourove ◽

Pappu Dey

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanol Extract ◽

Scavenging Activity ◽

Dpph Radical ◽

Tannin Content ◽

Dpph Radical Scavenging Activity ◽

Dpph Radical Scavenging

This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used.

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PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF CORCHORUS OLITORIUS

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2017v9i5.22138 ◽

2017 ◽

pp. 59-63

Author(s):

Abdul Sadat ◽

Mayukh Hore ◽

Kaushik Chakraborty ◽

Subhrajyoti Roy

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Phytochemical Analysis ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Dpph Radical Scavenging

Objective: The present study was carried out to evaluate the in vitro preliminary phytochemical analysis and antioxidant activity of methanolic leaf extracts of Corchorus olitorius L. The in vitro antioxidant activity was evaluated by DPPH radical scavenging activity method.Methods: Fresh jute leaves (immature, mature and senescence) were collected, air dried and the crude powder was prepared for phytochemical and antioxidant analysis. The powder was mixed with 70% methanol and the supernatant was separated. The antioxidant activity of this methanolic extract was measured on the basis of the scavenging activity of the stable 1, 1-diphenyl 2-picrylhydrazyl (DPPH) free radical with slight modifications. Phytochemical analysis was performed according to standard laboratory protocol.Results: The results indicated the presence of different phytochemicals viz. glycosides, steroids, cholesterol, alkaloids, phenols, flavonoids, riboflavin, saponins and terpenoids. The sample also showed antioxidant activity by inhibiting DPPH radical. The significant antioxidant activity of methanolic leaf extract of might be due to the presence of saponins, phenols, flavonoids and alkaloids found in the preliminary phytochemical analysis.Conclusion: Present study reveals that the jute leaf possesses different phytonutrients and exhibited DPPH radical scavenging activity, and therefore, may be used for therapeutic purposes.

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Aktivitas Penangkalan Radikal Bebas dan Kemampuan Reduksi Ekstrak Kulit Kayu Akway (Drimys piperita Hook. f.)

Jurnal Aplikasi Teknologi Pangan ◽

10.17728/jatp.3239 ◽

2019 ◽

Vol 7 (4) ◽

Cited By ~ 1

Author(s):

Gino Nemesio Cepeda ◽

Meike Meilan Lisangan ◽

Mathelda Kurniaty Roreng ◽

Elva Intan Permatasari ◽

Dolly Citra Manalu ◽

...

Keyword(s):

Free Radical ◽

Vitamin C ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Free Radical Scavenging ◽

Total Phenol ◽

Free Radical Scavenging Activity ◽

Scavenging Activity

Akway (Drimys piperita Hook. f) merupakan tumbuhan yang termasuk dalam kelompok tumbuhan berkayu, berdaun tebal aromatik dan termasuk kerabat winteraceae. Tumbuhan ini dimanfaatkan sebagai obat tradisional untuk mengobati malaria dan untuk meningkatkan vitalitas tubuh. Beberapa penelitian kandungan fitokimia ekstrak akway telah dilakukan untuk mengetahui potensi bioaktivitas akway. Ekstrak kulit kayu akway dilaporkan mengandung kelompok senyawa alkaloid, saponin, tanin, flavonoid, terpenoid dan glikosida. Minyak atsiri kulit kayu akway mengandung linalool, β-pinen, α-pinen, nerolidol dan terpineol. Senyawa-senyawa tersebut dilaporkan memiliki aktivitas antioksidan yang tinggi. Tujuan penelitian ini adalah untuk menentukan kapasitas antioksidan ekstrak kulit kayu akway secara in vitro dan vitamin C sebagai kontrol positif. Pengujian kapasitas antioksidan yang dilakukan meliputi kandungan total fenol dengan metode Folin-Ciocalteu, kandungan flavonoid dengan metode aluminum klorida, kapasitas penangkalan radikal bebas menggunakan metode DPPH (1,1-Diphenyl-2-picrylhydrazyl)-radical scavenging assay dan daya reduksi menggunakan metode reduksi Fe+3 menjadi Fe+2. Hasil penelitian menunjukkan bahwa ekstrak metanol memiliki kandungan total fenol dan flavonoid yang tertinggi dibandingkan dengan ekstrak etanol dan etilasetat. Kandungan total fenol dan flavonoid ekstrak metanol masing-masing sebesar 18,22 dan 14,32%. Ekstrak metanol dan vitamin C memiliki kapasitas menangkal radikal bebas DPPH dan daya reduksi yang paling tinggi kemudian diikuti ekstrak etanol dan etilasetat. Kemampuan menangkal radikal bebas ekstrak metanol dan vitamin C pada konsentrasi 200 µg/ml masing-masing sebesar 90% dan 88,31% sedangkan daya reduksi masing-masing sebesar 0,54 dan 0,62. Kesimpulannya, ekstrak metanol memiliki kapasitas antioksidan yang paling tinggi dibandingkan dengan ekstrak etanol dan etilasetat. Abstract Free Radical Scavenging Activity and Reducing Power of Akway (Drimys piperita Hook. f.) Bark ExtractsAkway (Drimys piperita Hook. f) was a woody and aromatic plant of winteraceae. This plant was used as traditional medical plant to heal malaria and to enhance vitality of body. Some studies were done to know bioactivity potency of akway extracts. D. piperita bark extract contains alkaloid, saponin, tanin, flavonoid, terpenoid and glycoside. The bark essential oil of the plant consists of lynalool, β-pinene, α-pinene, nerolidol and terpineol. Those compounds were exhibited high antioxidant activity. The objectives of this research were to determine total phenol and flavonoid of the extracts and its antioxidant capacity which was compared to antioxidant capacity of vitamin C. The assay of antioxidant capacity comprised of total phenol and flavonoid content, free radical scavenging activity, and reducing power. Total phenol and flavonoid was determined using Folin-Ciocalteu and aluminum chloride method, respectively while determination of free radical scavenging activity and reducing power using DPPH-radical scavenging and Fe+3 to Fe+2 reducing power method, respectively. The results indicated that methanol extract had the highest in total phenol and flavonoid content. Total phenol and flavonoid of methanol extract were 18.22% and 14.32%, respectively. Vitamin C and methanol extract had the highest DPPH (1,1-Diphenyl-2-picrylhydrazyl)-radical scavenging activity and reducing power, respectively. DPPH-radical scavenging activity of methanol extract and vitamin C in concentration of 200 µg/ml were 90% and 88.31%, respectively while its reducing power were 0.54 and 0,62, respectively. As conclusion, methanol extract had the highest antioxidant activity compared with ethanol and ethyl acetate extracts.

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Phytochemical Contents and Antioxidant Activity of Medicinal Plants from the Rubiaceae Family in Thailand

Plant Science Today ◽

10.14719/pst.2021.8.1.882 ◽

2021 ◽

Vol 8 (1) ◽

pp. 24-31

Author(s):

Rungcharn Suksungworn ◽

Sutsawat Duangsrisai

Keyword(s):

Antioxidant Activity ◽

Plant Extracts ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Scavenging Activity ◽

Antioxidant Power ◽

Nitric Oxide Radical ◽

Promising Source ◽

Phytochemical Contents

Several plants of the Rubiaceae family possess potential pharmacological properties, such as antioxidant activity, for subsequent drug development. We investigated the methanolic extracts from the bark and wood of five Rubiaceae species for phenolic and flavonoid contents and antioxidant activity. Regarding the phytochemical contents and antioxidant activity, Mitragyna diversifolia wood (437.57 ± 9.90 mg GAE g-1) and Haldina cordifolia wood (30.11 ± 0.20 mg QE g-1) displayed the highest total phenolic content (TPC) and total flavonoid content (TFC) respectively. Morinda coreia bark followed the highest antioxidant activities (IC50 = 360.58 ± 19.28 µg ml-1) in the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), Catunaregam tomentosa bark (IC50 = 13.96 ± 5.32 µg ml-1) in the nitric oxide radical scavenging activity (NO), M. coreia wood (IC50 = 918.27 ± 0.16 µg ml-1) in the superoxide radical scavenging activity (SO) and M. coreia wood (IC50 = 236.65 ± 1.66 µg ml-1) in ferric reducing antioxidant power activity (FRAP). The TPC and TFC displayed strong correlations with DPPH in M. diversifolia wood and with FRAP in M. diversifolia bark and wood. We found high correlation between TFC and FRAP in all plant extracts except C. tomentosa wood, while no relation was detected between TFC and NO in all plant extracts. Comparing Rubiaceae species, the highest antioxidant potential were showed in C. tomentosa bark. Overall, it is worth mentioning that the Rubiaceae species exhibit potential as a promising source of natural antioxidants.

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Evaluation of Antioxidant Activity of fresh Lemon (Citrus lemon L.) peel in Marrakech, Kenitra cities of Morocco and Taiz of Yemen

Mediterranean Journal of Chemistry ◽

10.13171/mjc10602006261379ka ◽

2020 ◽

Vol 10 (6) ◽

pp. 585

Author(s):

Khaled Abdu ◽

Rahma Erahioui ◽

Amina Moutawalli ◽

Ahmed Zahidi ◽

Khadija Khedid ◽

...

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Lemon Peel ◽

Dpph Radical Scavenging

The principal objective of this study was to appraise the variation of antioxidant activity, total phenols, and total flavonoids of fresh lemon peel from regions located Marrakech and Kenitra in Morocco and a region Taiz in Yemen. The test of polyphenol was performed from extracts that were measured using the Folin-Ciocalteu technique. Also, an analysis of Flavonoid was performed of was measured by using the aluminum chloride. The Free radical scavenging activity of lemon extracts was measured by 2,2′-Diphenyl-1-picrylhydrazyl hydrate (DPPH).The DPPH radical scavenging activity of ethanolic extract of fresh lemon peel found to be highest at 200μl concentration, which was 77. 15 %; 67. 88 %; 37.74%. Through it, 50% inhibition (IC50) was obtained to be 92. 04; 153. 55 and 215.407 μg/ml in Marrakech, Kenitra, and Taiz, respectively. As well, the values radical scavenging activity of methanolic extract of the same concentration, which are 82, 77 %, 53, 33 %, and 47,5%. Through it, 50% inhibition (IC50) was obtained to be 145. 6; 179. 17 and 274.899 μg/ml in Marrakech, Kenitra, and Taiz, respectively. But regarding the values radical scavenging activity of essential oil of the same concentration, which is 63.77 %, 42.19 %, and 80.25%. Through it, 50% inhibition (IC50) was obtained to be 155.54; 271.158 and 101.31 μg/ml in Marrakech, Kenitra, and Taiz, respectively. According to the results obtained in our study, the antioxidant activity increases with increased polyphenols and IC50 deficiency in Marrakech, Kenitra, and Taiz.

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In vitro and in vivo Antioxidant Properties of Extracts from the Root of Curcuma longa Linn

European Journal of Medicinal Plants ◽

10.9734/ejmp/2020/v31i630241 ◽

2020 ◽

pp. 6-12

Author(s):

Abdulrashid Mohammed ◽

Muhammad Ibrahim Usman ◽

Alhassan Muhammad Wudil ◽

Adamu Jibrin Alhassan ◽

Salisu Maiwada Abubakar ◽

...

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Curcuma Longa ◽

Radical Scavenging ◽

Diabetic Rats ◽

Scavenging Activity ◽

Liver And Kidney

Many plants possess antioxidants that exhibit additive or synergistic activities. The antioxidant activities of the root of Curcuma longa Linn extracts extracted different solvents were investigated by using several established in vitro systems: α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen Peroxide scavenging activity (HPSA), nitric oxide radical scavenging activity (NOSA) and ferric reducing antioxidant power (FRAP). The result showed that methanol extract exhibited greater antioxidant activity in vitro which was statistically significant compared to the other extracts. Based on the in vitro results, the methanol extract was subjected to column chromatography. Six pooled fractions (FI-FVI) were evaluated for in vivo antioxidant activity in liver and kidney of alloxan-induced diabetic rats using a total of forty-five (45) rats which were grouped into nine (9) groups of five (5) rats. The in vivo antioxidants showed a significant decrease in superoxide dismutase (SOD), catalase (CAT) and gluthatione peroxidase (GPx) levels in both liver and kidney of Alloxan-induced diabetic rats. These changes were significantly reversed after treatment with methanol fraction II and the standard drug. Thus, Curcuma longa Linn may be useful in the management of diabetes and oxidative stress.

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ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15013 ◽

2017 ◽

Vol 9 (2) ◽

pp. 279 ◽

Cited By ~ 1

Author(s):

Ahmed R. Abu-raghif ◽

Ghaith A. Jasim ◽

Muneer M. Hanoon

Keyword(s):

Antioxidant Activity ◽

Cell Line ◽

Proliferative Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cancer Cell Line ◽

Extraction Yield ◽

Gas Chromatography Mass Spectrometry ◽

Scavenging Activity

Objective: The aim of this study was to investigate the capability of Zizyphus spina christi methanol extract to inhibit cancer cell line proliferation.Methods: The leaves of Zizyphus spina christi were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Results: The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC50 was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC50 of 33.91 mg/ml.Conclusion: Methanol extract showed potential anti-proliferative activity against RD cell line, which could be due to its antioxidant activity.

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Phytochemical Analysis and Antimicrobial/Antioxidant Activity of Cynodon dactylon (L.) Pers. Rhizome Methanolic Extract

Journal of Food Quality ◽

10.1155/2020/5946541 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Samira Savadi ◽

Mohsen Vazifedoost ◽

Zohre Didar ◽

Mohammad Mahdi Nematshahi ◽

Eisa Jahed

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Cynodon Dactylon ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Inhibition Zone ◽

Free Radical Scavenging ◽

Free Radical Scavenging Activity ◽

Scavenging Activity

A proper use of medicinal plants requires accurate scientific information and understanding of their chemical constituents. The therapeutic effects in the plants are due to the chemical compounds therein. Cynodon dactylon (L.) Pers. of the family Poaceae is a perennial, pan-tropical species of grass which is a well-known traditional medicine and has a renowned position for treatment of many symptoms and diseases. The chemical composition, free radical scavenging activity, and antimicrobial properties of the methanolic extract of C. dactylon rhizomes against selected bacterial and fungal strains were investigated using disk-diffusion method. The results indicated that the major fatty acid structures of C. dactylon methanolic extract were palmitic acid (36.40%), oleic acid (28.26%), and linoleic acid (17.01%). Alpha-tocopherol (151.39 mg·kg−1) and sitosterol (3199.62 mg·kg−1) were the main tocopherols and sterols, respectively. According to the instrumental analysis, the total phenolic compounds of methanolic extract were equal to 917.08 mg·kg−1 and the main compound was hydroquinone (66.89%). Antioxidant activity of the methanolic extract at concentrations of 100–1000 ppm was 9.81–67.87%, which is significantly different from the 200 ppm synthetic antioxidant (BHT) with free radical scavenging activity equal to 48.93% (p<0.05). The antimicrobial study revealed that the methanolic extract of C. dactylon rhizomes was effective against all of the bacterial pathogens and the antibacterial activity increased with an increase in the concentration of extract. Therefore, the largest zone of inhibition was observed against Bacillus cereus (18.3 ± 0.4 mm) and Escherichia coli (16.8 ± 0.5 mm) at 1000 ppm. The highest resistance was observed with Pseudomonas aeruginosa (inhibition zone of 12.8 ± 0.15 mm). Methanolic extract at 200 ppm had no effect against fungus Aspergillus niger. However, at 1000 ppm concentration, an inhibition zone with a diameter of 14.4 ± 0.45 mm was formed.

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