scholarly journals Molecular Diversity Analysis in Cotton (Gossypium hirsutum L.) Using RAPD Markers

2020 ◽  
pp. 33-44
Author(s):  
Md. Abdul Wahab ◽  
Shamim Ara Sumi ◽  
Absana Islam ◽  
M. E. Houqe
Keyword(s):  
Gossypium Hirsutum ◽  
Rapd Markers ◽  
Molecular Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Research Work ◽  
Dna Amplification ◽  
Gossypium Hirsutum L ◽  
Average Value ◽  
Cotton Genotypes ◽  
Selection Of

Cotton (Gossypium hirsutum L) is considered as major textile fiber around the world. This research aimed to analyze the molecular diversity among 9 cotton genotypes collected from Cotton Development Board, Bangladesh using the 7 Random Amplified Polymorphic DNA (RAPD) markers. Some total of 18 DNA bands were obtained and among them, 13 were polymorphic bands. The range of DNA amplification varied from 180 to 800 bp. The percentage of polymorphism was about 50.71. Genetic diversity ranged from 0.22 to 0.44 with an average value of 0.34. Nei’s genetic distance ranging from 0.1667 to 0.6667 and most importantly PIC value ranged from 0.18 to 0.35 with an average value of 0.27. The PIC value indicated that most of the studied cotton genotypes were moderately diversified and homogenous as well as no heterozygosity found. A dendrogram indicating the relative genetic similarity of the Bangladeshi cotton genotypes was constructed which followed in two major clusters (A and B) among the studied material. The Cotton Development Board (CDB), Bangladesh committed to working on cotton improvement and this investigation will be helpful for the selection of diversified genotypes in varietal improvement in cotton. The output of this research will be a baseline for future molecular research work on cotton genotypes in Bangladesh.

Phylogenetic grouping and identification of Rhizobium isolates on the basis of random amplified polymorphic DNA profiles

1993 ◽  
Vol 39 (7) ◽  
pp. 665-673 ◽  
Author(s):  
John J. Dooley ◽  
Stephen P. Harrison ◽  
Lance R. Mytton ◽  
Malcolm Dye ◽  
Ann Cresswell ◽  
...  
Keyword(s):  
Rhizobium Leguminosarum ◽  
Rhizobium Meliloti ◽  
Random Amplified Polymorphic Dna ◽  
Dna Amplification ◽  
Distinct Group ◽  
Principal Coordinate Analysis ◽  
The Other ◽  
Phylogenetic Grouping ◽  
Dna Profiles ◽  
Selection Of

Through the use of a single, random 15mer as a primer, between 1 and 12 DNA amplification products were obtained per strain from a selection of 84 Rhizobium and Bradyrhizobium isolates. A principal-coordinate analysis was used to analyse the resulting amplified DNA profiles and it was possible to assign isolates to specific groupings. Within the species Rhizobium leguminosarum, the biovar phaseoli formed a distinct group from the other biovars of the species, viciae and trifolii, which grouped together. Isolates of Rhizobium meliloti and Bradyrhizobium species formed their own clear, specific groups. Although it was possible to identify individual isolates on the basis of differences in their amplified DNA profiles, there was evidence that some amplified segments were conserved among individuals at the biovar and species levels.Key words: Rhizobium, DNA amplification, random primers.

scholarly journals Variability of morphometric indicators of honey bees bred in Almaty region

2021 ◽  
Author(s):  
U.A. Nuralieva ◽  
A.A. Baisabyrova ◽  
G.A. Moldakhmetova ◽  
K.A. Temirbayeva ◽  
R.Zh. Shimelkova ◽  
...  
Keyword(s):  
Honey Bees ◽  
Selection Process ◽  
Research Work ◽  
Effective Management ◽  
Ministry Of Education ◽  
Average Value ◽  
The Republic ◽  
Efficiency Of Selection ◽  
Bee Colonies ◽  
Selection Of

One of the ways to intensify the production of beekeeping products is selection. Bee breeding is not only one of the most important methods, but also the most economically efficient way to increase the productivity of bee colonies. Thus, the selection of bees and the implementation of its achievements into production are one of the most important and most effective directions for intensifying beekeeping. Research work was carried out under the project of program-targeted financing of the Ministry of Education and Science of the Republic of Kazakhstan on the topic "Development of technologies for effective management of the selection process in beekeeping." This article examines the characteristics of the morphometric indicators of honeybees in the Almaty region of the Republic of Kazakhstan. The material for the research was the specimens of worker bees from apiaries located in the Almaty region of the Devochkin farm, Panov farm, Kalinin Individual Entrepreneur, Adilgazy Individual Entrepreneur, Kashkimbaev farm. To carry out the study according to the method of A.B. Kartashev, 35 samples of bees were worked out. Changes in the parameters of the wings, including the cubital and dumbbell index, discoidal displacement by bee species: Central Russian, Carpathian, Italian and Carniolian honey bee, are considered. It was found that in Kalinin’s apiary morphometric indicators for the cubital index, the average value was 2,787%. As a result, the morphometric indices for the cubital index in bees of the IP Kalinin bee were 2.777%. Whereas in other farms, the average value was significantly lower for all indicators. Accordingly, the percentage of the cubital index was 7.42-17.36%, the dumbbell index was 6.77-11.81%, and the discoidal displacement was 32.91-47.37%. According to all indicators, it is clear that the Kalinin Individual Entrepreneur’s bee farm is superior to other bee farms in terms of morphometric data. This is due to the isolation of the beekeeping and out of reach of other bees, thus ensuring a low level of hybridization. The considered analysis of the species belonging to the entire apiary, as well as economically useful features, can significantly increase the efficiency of selection work in beekeeping.

Germ line genetic transformation in cotton (Gossypium hirsutum L.) by selection of transgenic meristematic cells with a herbicide molecule

Plant Science ◽  
2005 ◽  
Vol 168 (5) ◽  
pp. 1227-1233 ◽  
Author(s):  
Francisco J.L. Aragão ◽  
Giovanni R. Vianna ◽  
Silvia B.R.C. Carvalheira ◽  
Elibio L. Rech
Keyword(s):  
Genetic Transformation ◽  
Gossypium Hirsutum ◽  
Germ Line ◽  
Gossypium Hirsutum L ◽  
Meristematic Cells ◽  
Selection Of

Genetic fingerprinting of Australian cotton cultivars with RAPD markers

Genome ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 1005-1008 ◽  
Author(s):  
D. S. Multani ◽  
B. R. Lyon
Keyword(s):  
Cluster Analysis ◽  
Gossypium Hirsutum ◽  
Dna Markers ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pairwise Comparisons ◽  
Breeding Line ◽  
Genetic Fingerprinting ◽  
Broad Agreement

RAPD (random amplified polymorphic DNA) markers generated by 30 random decamer primers were used to fingerprint 12 released cultivars and a breeding line of Gossypium hirsutum and 1 cultivar of G. barbadense presently under cultivation in Australia. Among a total of 453 developed markers, 69 (15.2%) were only present (unique) in the G. barbadense cultivar Pima S-7. Of the remaining markers, 128 (33.3%) were fixed in all 13 G. hirsutum cultivars. In pairwise comparisons of the degree of band sharing, nine closely-related cultivars showed 92.1–98.9% genetic similarity. Cluster analysis of genetic distance estimates between each of the cultivars revealed phylogenetic relationships in broad agreement with the known lineage of the cultivars. Ten of the G. hirsutum cultivars can be characterized individually based upon cultivar-specific RAPD markers, thus making it possible to differentiate closely related cultivars by molecular markers.Key words: RAPD, DNA fingerprinting, Gossypium hirsutum, Gossypium barbadense, cotton cultivars.

scholarly journals Molecular Diversity and Association Analysis of Drought and Salt Tolerance in Gossypium hirsutum L. Germplasm

2014 ◽  
Vol 13 (9) ◽  
pp. 1845-1853 ◽  
Author(s):  
Yin-hua JIA ◽  
Jun-ling SUN ◽  
Xi-wen WANG ◽  
Zhong-li ZHOU ◽  
Zao-e PAN ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Gossypium Hirsutum ◽  
Association Analysis ◽  
Molecular Diversity ◽  
Gossypium Hirsutum L ◽  
Drought And Salt Tolerance

scholarly journals ESTRATÉGIAS DE MANEJO DO DÉFICIT HÍDRICO EM FASES FENOLÓGICAS DO ALGODOEIRO COLORIDO

Irriga ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 656-662
Author(s):  
Lauriane Almeida dos Anjos Soares ◽  
KAROLINE MARIA MENDES DIAS ◽  
HEMILE MACHADO NASCIMENTO ◽  
GEOVANI SOARES DE LIMA ◽  
KELDER JOSÉ ALVES DE OLIVEIRA ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Water Deficit ◽  
Irrigated Agriculture ◽  
Limiting Factor ◽  
Gossypium Hirsutum L ◽  
Root Shoot Ratio ◽  
Colored Cotton ◽  
Cotton Genotypes ◽  
E Mail ◽  
Phenological Phases

ESTRATÉGIAS DE MANEJO DO DÉFICIT HÍDRICO EM FASES FENOLÓGICAS DO ALGODOEIRO COLORIDO     LAURIANE ALMEIDA DOS ANJOS SOARES1; KAROLINE MARIA MENDES DIAS2; HEMILE MACHADO NASCIMENTO3; GEOVANI SOARES DE LIMA4; KELDER JOSÉ ALVES DE OLIVEIRA5 E SAULO SOARES DA SILVA6   1 Unidade Acadêmica de Ciências Agrárias, Universidade Federal de Campina Grande, UFCG, Rua Jario Vieira Feitosa, 1770, Bairro dos Pereiros, Pombal, PB, Brasil. E-mail: [email protected] 2 Unidade Acadêmica de Ciências Agrárias, Universidade Federal de Campina Grande, UFCG, Rua Jario Vieira Feitosa, 1770, Bairro dos Pereiros, Pombal, PB, Brasil. E-mail: [email protected]. 3 Unidade Acadêmica de Ciências Agrárias, Universidade Federal de Campina Grande, UFCG, Rua Jario Vieira Feitosa, 1770, Bairro dos Pereiros, Pombal, PB, Brasil. E-mail: [email protected] 4 Unidade Acadêmica de Engenharia Agrícola, Universidade Federal de Campina Grande, UFCG, Rua Aprígio Veloso, 882 -Universitário, Campina Grande, PB, Brasil. E-mail: [email protected] 5 Unidade Acadêmica de Ciências Agrárias, Universidade Federal de Campina Grande, UFCG, Rua Jario Vieira Feitosa, 1770, Bairro dos Pereiros, Pombal, PB, Brasil. E-mail: [email protected] 6 Unidade Acadêmica de Engenharia Agrícola, Universidade Federal de Campina Grande, UFCG, Rua Aprígio Veloso, 882 -Universitário, Campina Grande, PB, Brasil. E-mail: [email protected]     1 RESUMO   A escassez hídrica é um fator limitante para a agricultura irrigada, principalmente em algumas fases do algodoeiro, afetando os processos fisiológicos e consequentemente a produtividade. Neste contexto, objetivou-se com este trabalho avaliar a fitomassa e os componentes de produção de genótipos de algodoeiro colorido sob déficit hídrico nas diferentes fases de fenológicas da planta. As plantas foram conduzidas em lisímetros sob condições de campo, no Centro de Ciências e Tecnologia Agroalimentar pertencente à Universidade Federal de Campina Grande, na cidade de Pombal-PB. O delineamento utilizado foi em blocos ao acaso em esquema fatorial 3 x 3, sendo três genótipos de algodão de fibra colorida (BRS Rubi, BRS Topázio e BRS Safira) irrigados sob déficit hídrico durante as três fases de desenvolvimento da cultura (vegetativa, floração e frutificação). A relação raiz/parte aérea é mais sensível aos efeitos do déficit hídrico na fase de floração e formação dos capulhos. Dentre os genótipos o BRS Topázio é o mais tolerante ao déficit hídrico (40% da ETr) durante a fase vegetativa para o número de sementes totais. O déficit hídrico na fase de frutificação do algodoeiro colorido pode ser utilizado no cultivo do algodoeiro com as menores perdas no peso de 100 sementes.   Palavras-chave: Gossypium hirsutum L., fitomassa, produção     SOARES, L. A. dos A.; DIAS, K. M. M.; NASCIMENTO, H. M.; LIMA, G. S. de OLIVEIRA, K. J. A. de; SILVA, S. S. da STRATEGIES FOR THE MANAGEMENT OF WATER DEFICIT IN PHENOLOGICAL PHASES OF COLORED COTTON   2 ABSTRACT   Water scarcity is a limiting factor for irrigated agriculture, mainly in some phases of cotton, affecting physiological processes and consequently productivity. In this context, this work aimed to evaluate the phytomass and production components of colored cotton genotypes under water deficit in different phenological phases of the plant. The plants were conducted in lysimeters under field conditions, at the Center for Science and Agrifood Technology belonging to the Federal University of Campina Grande, in the city of Pombal-PB. The design used was in a randomized block in a 3 x 3 factorial scheme, with three colored fiber cotton genotypes (BRS Rubi, BRS Topázio and BRS Safira) irrigated under water deficit during the three stages of crop development (vegetative, flowering and fruiting). The root/shoot ratio is more sensitive to the effects of water deficit during flowering and boll formation. Among the genotypes, BRS Topázio is the most tolerant to water deficit (40% of ETr) during the vegetative phase for the number of total seeds. The water deficit in the fruiting stage of colored cotton can be used in the cultivation of cotton with the lowest losses in the weight of 100 seeds.   Keywords: Gossypium hirsutum L., phytomass, production

scholarly journals Molecular diversity of Pakistani upland cotton (Gossypium hirsutum L.) varieties based on microsatellite markers

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Seema Aslam ◽  
Huma Iqbal ◽  
Mujahid Hussain ◽  
Muhammad Baber
Keyword(s):  
Genetic Diversity ◽  
Gossypium Hirsutum ◽  
Upland Cotton ◽  
Molecular Diversity ◽  
Genetic Relatedness ◽  
Genome Mapping ◽  
Gossypium Hirsutum L ◽  
Low Genetic Diversity ◽  
Comparative Genome Mapping ◽  
The Mean

For the management of germplasm and varietal characterization the understanding and estimation of genetic diversity plays a key role. In this study genetic diversity of thirty Pakistani Upland cotton (Gossypium hirsutum L.) varieties was determined by using thirteen SSR markers as these markers are highly polymorphic and efficient for comparative genome mapping. Total 48 band were produced by all thirteen markers; five markers were monomorphic and eight were polymorphic. Highest polymorphism was 68% that produced by NAU 2083 marker. To find the genetic relatedness between thirty studied varieties UPGMA was used for the construction of dendrogram. The similarity between studied thirty varieties ranged from 38.46% to 100%. Highest pic value was 0.6484 shown by NAU 2083 and the mean pic value was 0.2833 which predicted that very low genetic diversity is present among the cotton varieties under investigation.

scholarly journals Identification of Random Amplified Polymorphic DNA (RAPD) Markers for Self-incompatibility Alleles in Hazelnut

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 591e-591
Author(s):  
Kirk W. Pomper ◽  
Anita N. Azarenko ◽  
Joel W. Davis ◽  
Shawn A. Mehlenbacher
Keyword(s):  
Rapd Markers ◽  
Uv Light ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Self Incompatibility ◽  
Ethidium Bromide Staining ◽  
Young Leaves ◽  
Incompatibility Alleles ◽  
Selection Of

Random amplified polymorphic DNA (RAPD) markers were identified for self-incompatibility (SI) alleles that will allow marker-assisted selection of desired S-alleles and assist in cloning the locus responsible for the sporophytic SI displayed in hazelnut (Corylus avellana L.). DNA was extracted from young leaves collected from field-planted parents and 27 progeny of the cross OSU 23.017 (S1 S12) × VR6-28 (S2 S26). Screening of 10-base oligonucleotide RAPD primers was performed using bulked segregant analysis. DNA samples from six trees each were pooled into four “bulks,” one for each of the following: S1 S2, S1 S26, S2 S12, and S12 S26. “Super bulks” of twelve trees each for S1, S2, S12, and S26 then were created for each allele by combining the appropriate bulks. The DNA from these four super bulks and also the parents was used as a template in the PCR assays. Amplification products were electrophoresed on 2% agarose gels and photographed under UV light after ethidium bromide staining. 200 primers were screened and one RAPD marker each was identified for alleles S2 (OPI-07700) and S1 (OPJ-141700).

scholarly journals Genetic Distance and Variation among Cotton (Gossypium hirsutum. L) Genotypes for Yield Traits in Kamashi District of Benishangul-Gumuz Regional State, Ethiopia

2019 ◽  
Vol 7 (4) ◽  
pp. 91-100
Author(s):  
Kedir Wulchafo Hussen
Keyword(s):  
Gossypium Hirsutum ◽  
Genetic Distance ◽  
Block Design ◽  
Yield Traits ◽  
Gossypium Hirsutum L ◽  
Mean Values ◽  
Regional State ◽  
Wide Range ◽  
Randomized Complete Block Design ◽  
Cotton Genotypes

The present research was carried out to determine and record genetic distance and variation among cotton (Gossypium hirsutum L) genotypes for yield traits in Kamashi district of benishangul-gumuz regional state for fourteen upland cotton genotypes. The genotypes were evaluated for ten traits in randomized complete block design with three replications. Data were collected on days of 50% flowering, days to 65%boll opening, plant height, Number of monopodial branches per plant, Number of sympodial branches per plant, Boll number per plant, Boll weight Seed cotton yield per hectare, Lint yield and Ginning out tern. The data were subjected to analysis of variance. Genotypes were varied significantly for most of the traits studied and there were wide range variation on mean values for most of the traits which indicated the presence of variability among the tested genotypes.

Sign in / Sign up

Export Citation Format

Share Document