Probiotic Potential and Genomic Evaluation of Lactic Acid Bacteria Isolated from Fermented Sorghum-Based Gruel

Forty eight isolates were randomly isolated from fermented red sorghum (Sorghum bicolor) and white sorghum-based (Sorghum vulgare) gruel and evaluated for their probiotic abilities. Forty five out of the forty eight isolates were Gram-positive, catalase negative and non-sporulating, which were further evaluated. Twenty out of the forty five isolates showed tolerance to acid with their ability to survive extreme pH 2.0, 3.0 and 4.0 respectively at different levels. These were further screened for their in vitro inhibition ability against selected test pathogens (Escherichia coli, Salmonella Typhimurium, Bacillus sp., Pseudomonas aeruginosa, and Staphylococcus aureus). Fourteen out of the twenty isolates had antimicrobial activity against the test pathogens at different levels. They were further screened for their antibiotic susceptibility pattern, all the fourteen isolates showed susceptibility to different antibiotics at different levels. These isolates were screened for their bile tolerance ability, all the fourteen isolates showed tolerance to the bile salt concentration (0.3%, 0.5% and 1%) at different levels respectively. The fourteen isolates were also screened for their gastric transit tolerance ability; all the fourteen isolates were able to tolerate gastric transit at 0 minute, 90 minutes and 180 minutes respectively. They were further screened for their in vitro hydrophobicity ability, only six out of the fourteen isolates showed percentage hydrophobicity of 40% and above. These six isolates were identified at the sub-species level using 16S RRNA Sequencing for isolates identification and construction of phylogeny. They were identified as Lactobacillus plantarum strain ST1, Lactobacillus brevis strain SC4, Lactobacillus casei strain 0108, Lactobacillus plantarum strain HASOB9a, Pediococcus pentosaceus strain SH 740 and Lactobacillus plantarum ZJ316 respectively. Out of the six identified isolates, the complete genome of Lactobacillus plantarum ZJ316 had been sequenced and was retrieved alongside with the complete genome data of four other species of Lactobacillus plantarum of industrial importance available in public domain, which was used for the mini comparative genome analysis in this research, using bioinformatics and visual analytics tools (Tableau Desktop). It was revealed that the genome size of Lactobacillus plantarum ZJ316 is second to that of Lactobacillus plantarum 16 and higher than that of the others whose genome had been completely sequenced.

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Large Plasmid Complement Resolved: Complete Genome Sequencing of Lactobacillus plantarum MF1298, a Candidate Probiotic Strain Associated with Unfavorable Effect

Microorganisms ◽

10.3390/microorganisms7080262 ◽

2019 ◽

Vol 7 (8) ◽

pp. 262

Author(s):

McLeod ◽

Fagerlund ◽

Rud ◽

Axelsson

Keyword(s):

Lactobacillus Plantarum ◽

Complete Genome ◽

Probiotic Strain ◽

General Interest ◽

Probiotic Properties ◽

Large Plasmid ◽

Reverse Transcriptases ◽

Probiotic Potential ◽

Unfavorable Effect

Considerable attention has been given to the species Lactobacillus plantarum regarding its probiotic potential. L. plantarum strains have shown health benefits in several studies, and even nonstrain-specific claims are allowed in certain markets. L. plantarum strain MF1298 was considered a candidate probiotic, demonstrating in vitro probiotic properties and the ability to survive passage through the human intestinal tract. However, the strain showed an unfavorable effect on symptoms in subjects with irritable bowel syndrome in a clinical trial. The properties and the genome of this strain are thus of general interest. Obtaining the complete genome of strain MF1298 proved difficult due to its large plasmid complement. Here, we exploit a combination of sequencing approaches to obtain the complete chromosome and plasmid assemblies of MF1298. The Oxford Nanopore Technologies MinION long-read sequencer was particularly useful in resolving the unusually large number of plasmids in the strain, 14 in total. The complete genome sequence of 3,576,440 basepairs contains 3272 protein-encoding genes, of which 315 are located on plasmids. Few unique regions were found in comparison with other L. plantarum genomes. Notably, however, one of the plasmids contains genes related to vitamin B12 (cobalamin) turnover and genes encoding bacterial reverse transcriptases, features not previously reported for L. plantarum. The extensive plasmid information will be important for future studies with this strain.

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Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽

10.3390/pr9050879 ◽

2021 ◽

Vol 9 (5) ◽

pp. 879

Author(s):

Seong-Shin Lee ◽

Jeong-Seok Choi ◽

Dimas Hand Vidya Paradhipta ◽

Young-Ho Joo ◽

Hyuk-Jun Lee ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Dry Matter ◽

Lactobacillus Brevis ◽

Neutral Detergent Fiber ◽

Dry Matter Digestibility ◽

Fiber Digestibility ◽

Aerobic Stability ◽

Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

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Complete Genome Sequence of Lanthionine-Producing Lactobacillus brevis Strain 100D8, Generated by PacBio Sequencing

Microbiology Resource Announcements ◽

10.1128/mra.01220-18 ◽

2018 ◽

Vol 7 (14) ◽

Cited By ~ 1

Author(s):

Min-Jung Kim ◽

Hye Sun Kim ◽

Sam Churl Kim ◽

Youn-Sig Kwak

Keyword(s):

Antifungal Activity ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Lactobacillus Brevis ◽

Pacbio Sequencing ◽

Circular Chromosome ◽

Coding Sequences ◽

Content Type

Lactobacillus brevis strain 100D8 was isolated from rye silage and showed rapid acidification ability in vitro and antifungal activity against mycotoxin-producing fungi. We report here the complete genome sequence of L. brevis strain 100D8, which has a circular chromosome (2,351,988 bp, 2,304 coding sequences [CDSs]) and three plasmids (45,061 bp, 57 CDSs; 40,740 bp, 40 CDSs; and 39,943 bp, 57 CDSs).

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Colonisation potential of plantaricin-producing Lactobacillus plantarum SF9C andS-layer-carrying Lactobacillus brevis SF9B among gut microbiota

10.21203/rs.2.23400/v2 ◽

2020 ◽

Author(s):

Katarina Butorac ◽

Martina Banic ◽

Jasna Novak ◽

Andreja Leboš Pavunc ◽

Ksenija Uroic ◽

...

Keyword(s):

Gut Microbiota ◽

Lactobacillus Plantarum ◽

Lactobacillus Brevis ◽

Illumina Miseq ◽

Rrna Gene ◽

Microbiota Composition ◽

Combined Application ◽

Probiotic Potential ◽

Gut Microbiota Composition

Abstract Background: The influence of an S-layer-carrying strain Lactobacillus brevis SF9B and a plantaricin-producing strain Lactobacillus plantarum SF9C on the gut microbiota composition was evaluated in the rats. Considering the probiotic potential of Lb. brevis SF9B, this study aimed to examine the antibacterial activity of Lb. plantarum SF9C and potential for their in vivo colonisation, which could be the basis for the investigation of their synergistic functionality. Results: A plantaricin-encoding cluster was identified in Lb. plantarum SF9C, a strain which efficiently inhibited the growth of Listeria monocytogenes ATCC®19111™ and Staphylococcus aureus 3048. Contrary to the plantaricin-producing SF9C strain, the S-layer-carrying SF9B strain excluded Escherichia coli 3014 and Salmonella enterica serovar Typhimurium FP1 from adhesion to Caco-2 cells. Finally, DGGE analysis of the V2-V3 region of the 16S rRNA gene confirmed the transit of two selected lactobacilli through the gastrointestinal tract (GIT). Microbiome profiling via the Illumina MiSeq platform revealed the prevalence of Lactobacillus spp. in the gut microbiota of rats suggesting their colonisation potential in GIT.Conclusion: The combined application of Lb. plantarum SF9C and Lb. brevis SF9B could influence the intestinal microbiota composition, which is reflected through the increased abundance of Lactobacillus genus, but also through altered abundances of other bacterial genera, either in the model of healthy or aberrant microbiota of rats. The obtained results contributed to the functional aspects of SF9C and SF9B strains which could be incorporated in the probiotic-containing functional foods and therefore have a beneficial influence on the gut microbiota composition.

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Utilização de bactérias ácido-lácticas isoladas do trato intestinal de tilápia-do-nilo como probiótico

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2008000900015 ◽

2008 ◽

Vol 43 (9) ◽

pp. 1201-1207 ◽

Cited By ~ 26

Author(s):

Adolfo Jatobá ◽

Felipe do Nascimento Vieira ◽

Celso Buglione Neto ◽

Bruno Corrêa Silva ◽

José Luís Pedreira Mouriño ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Lactobacillus Brevis ◽

Enterococcus Durans

O objetivo deste trabalho foi isolar bactérias ácido-lácticas do intestino de tilápias-do-nilo, e avaliar seu potencial probiótico. Foram isoladas cepas de bactérias ácido-lácticas, e foi avaliada a inibição aos patógenos in vitro. As cepas com os melhores resultados foram identificadas e utilizadas no experimento de colonização do trato intestinal de tilápias-do-nilo, via suplementação na dieta, em delineamento inteiramente ao acaso, com três tratamentos e quatro repetições. Foram avaliados: o total de bactérias, as bactérias ácido-lácticas, Vibrio ssp. e Pseudomonas ssp. A cepa com melhor resultado foi utilizada na infecção experimental, em delineamento inteiramente ao acaso, em esquema fatorial 2x3: dieta suplementada com a cepa e dieta-controle; e os peixes não submetidos à injeção, peixes submetidos à injeção de solução salina e à injeção de Enterococcus durans, com três repetições. Foram avaliados os parâmetros hematológicos. As duas cepas identificadas foram: Lactobacillus plantarum e Lactobacillus brevis, que colonizaram o trato intestinal de tilápias, contudo L. plantarum teve menor número total de bactérias e de Pseudomonas ssp. Foi observado maior número total de eritrócitos, trombócitos, leucócitos, linfócitos, neutrófilos e monócitos, em peixes alimentados com L. plantarum e submetidos à injeção de E. durans. O L. plantarum tem efeito probiótico e melhora o sistema imune das tilápias.

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Lactobacillus plantarum ZJ316 improves the quality of Stachys sieboldii Miq. pickle by inhibiting harmful bacteria growth and degrading nitrite, promoting the gut microbiota health in vitro

Food & Function ◽

10.1039/d1fo03025f ◽

2022 ◽

Author(s):

Shu Ting Hang ◽

Ling zhou Zeng ◽

Jia run Han ◽

Zhong qin Zhang ◽

Qingqing Zhou ◽

...

Keyword(s):

Quality Control ◽

Lactic Acid ◽

Lactobacillus Plantarum ◽

Microbial Contamination ◽

Nitrite Accumulation ◽

Bacteria Growth ◽

Lactobacillus Plantarum Zj316 ◽

Stachys Sieboldii

Microbial contamination and nitrite accumulation are two major concerns on the quality control of fermented vegetables. In the present study, a lactic acid bacteria strain Lactobacillus plantarum ZJ316 (ZJ316) was...

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In-vitro assessment of probiotic potential of Lactobacillus plantarum WU-P19 isolated from a traditional fermented herb

Annals of Microbiology ◽

10.1007/s13213-017-1318-7 ◽

2017 ◽

Vol 68 (2) ◽

pp. 79-91 ◽

Cited By ~ 13

Author(s):

Wilawan Palachum ◽

Yusuf Chisti ◽

Wanna Choorit

Keyword(s):

Lactobacillus Plantarum ◽

Probiotic Potential ◽

In Vitro Assessment

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In Vitro Characterization of Indigenous Probiotic Strains Isolated from Colombian Creole Pigs

Animals ◽

10.3390/ani10071204 ◽

2020 ◽

Vol 10 (7) ◽

pp. 1204 ◽

Cited By ~ 2

Author(s):

César Betancur ◽

Yordan Martínez ◽

Guillermo Tellez-Isaias ◽

Mavir Carolina Avellaneda ◽

Borja Velázquez-Martí

Keyword(s):

Lactobacillus Plantarum ◽

Lactobacillus Brevis ◽

Coli Strain ◽

Good Growth ◽

Biochemical Tests ◽

Enteropathogenic Bacteria ◽

In Vitro Characterization ◽

Serovar Typhimurium ◽

Probiotic Strains

Three lactic acid strains were isolated from feces of the native Zungo Pelado breed of pigs (n = 5) and presumably identified as belonging to the Lactobacillaceae family by morphological techniques showing that they were Gram-positive/rod-shaped and catalase- and oxidase-negative. They were then identified by biochemical tests using API 50CHL as Lactobacillus plantarum (CAM6), Lactobacillus brevis (CAM7), and Lactobacillus acidophilus (CL4). However, 16S rRNA identification showed that all three strains were Lactobacillus plantarum. Additionally, all three isolates were able to grow in pH 3 and 4. Interestingly, the growth of the CAM7 strain decreased at pH 5.6 compared to that of the CAM6 strain (p < 0.05), and the growth of the CL4 strain was reduced at pH 7(p < 0.05). All three candidates showed good growth on bile salts (≥0.15%), and CAM6 and CAM7 showed better tolerance at higher concentrations (0.30%). Similarly, all strains tolerated sodium chloride (NaCl) concentrations from 2 to 10%. These strains also grew well at all temperatures tested (30, 37, and 42 °C). The CAM6 strain showed in vitro antibacterial activity against selected enteropathogenic bacteria (Escherichia coli strain NBRC 102203 and Salmonella enterica serovar Typhimurium 4.5.12) and commensal bacteria (Klebsiella pneumoniae ATCC BAA-1705D-5 and Pseudomonas aeruginosa ATCC 15442) and resistance to all antibiotics except amoxicillin. Further studies to evaluate the effects of these probiotic candidate strains in commercial pigs are currently underway.

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