scholarly journals In Vitro Characterization of Indigenous Probiotic Strains Isolated from Colombian Creole Pigs

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1204 ◽  
Author(s):  
César Betancur ◽  
Yordan Martínez ◽  
Guillermo Tellez-Isaias ◽  
Mavir Carolina Avellaneda ◽  
Borja Velázquez-Martí
Keyword(s):  
Lactobacillus Plantarum ◽  
Lactobacillus Brevis ◽  
Coli Strain ◽  
Good Growth ◽  
Biochemical Tests ◽  
Enteropathogenic Bacteria ◽  
In Vitro Characterization ◽  
Serovar Typhimurium ◽  
Probiotic Strains

Three lactic acid strains were isolated from feces of the native Zungo Pelado breed of pigs (n = 5) and presumably identified as belonging to the Lactobacillaceae family by morphological techniques showing that they were Gram-positive/rod-shaped and catalase- and oxidase-negative. They were then identified by biochemical tests using API 50CHL as Lactobacillus plantarum (CAM6), Lactobacillus brevis (CAM7), and Lactobacillus acidophilus (CL4). However, 16S rRNA identification showed that all three strains were Lactobacillus plantarum. Additionally, all three isolates were able to grow in pH 3 and 4. Interestingly, the growth of the CAM7 strain decreased at pH 5.6 compared to that of the CAM6 strain (p < 0.05), and the growth of the CL4 strain was reduced at pH 7(p < 0.05). All three candidates showed good growth on bile salts (≥0.15%), and CAM6 and CAM7 showed better tolerance at higher concentrations (0.30%). Similarly, all strains tolerated sodium chloride (NaCl) concentrations from 2 to 10%. These strains also grew well at all temperatures tested (30, 37, and 42 °C). The CAM6 strain showed in vitro antibacterial activity against selected enteropathogenic bacteria (Escherichia coli strain NBRC 102203 and Salmonella enterica serovar Typhimurium 4.5.12) and commensal bacteria (Klebsiella pneumoniae ATCC BAA-1705D-5 and Pseudomonas aeruginosa ATCC 15442) and resistance to all antibiotics except amoxicillin. Further studies to evaluate the effects of these probiotic candidate strains in commercial pigs are currently underway.

scholarly journals Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Lactobacillus Brevis ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Fiber Digestibility ◽  
Aerobic Stability ◽  
Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

scholarly journals Utilização de bactérias ácido-lácticas isoladas do trato intestinal de tilápia-do-nilo como probiótico

2008 ◽  
Vol 43 (9) ◽  
pp. 1201-1207 ◽  
Author(s):  
Adolfo Jatobá ◽  
Felipe do Nascimento Vieira ◽  
Celso Buglione Neto ◽  
Bruno Corrêa Silva ◽  
José Luís Pedreira Mouriño ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Lactobacillus Brevis ◽  
Enterococcus Durans

O objetivo deste trabalho foi isolar bactérias ácido-lácticas do intestino de tilápias-do-nilo, e avaliar seu potencial probiótico. Foram isoladas cepas de bactérias ácido-lácticas, e foi avaliada a inibição aos patógenos in vitro. As cepas com os melhores resultados foram identificadas e utilizadas no experimento de colonização do trato intestinal de tilápias-do-nilo, via suplementação na dieta, em delineamento inteiramente ao acaso, com três tratamentos e quatro repetições. Foram avaliados: o total de bactérias, as bactérias ácido-lácticas, Vibrio ssp. e Pseudomonas ssp. A cepa com melhor resultado foi utilizada na infecção experimental, em delineamento inteiramente ao acaso, em esquema fatorial 2x3: dieta suplementada com a cepa e dieta-controle; e os peixes não submetidos à injeção, peixes submetidos à injeção de solução salina e à injeção de Enterococcus durans, com três repetições. Foram avaliados os parâmetros hematológicos. As duas cepas identificadas foram: Lactobacillus plantarum e Lactobacillus brevis, que colonizaram o trato intestinal de tilápias, contudo L. plantarum teve menor número total de bactérias e de Pseudomonas ssp. Foi observado maior número total de eritrócitos, trombócitos, leucócitos, linfócitos, neutrófilos e monócitos, em peixes alimentados com L. plantarum e submetidos à injeção de E. durans. O L. plantarum tem efeito probiótico e melhora o sistema imune das tilápias.

scholarly journals Potential Health Benefits of Ropy Exopolysaccharides Produced by Lactobacillus plantarum

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3293 ◽  
Author(s):  
Tülin Yılmaz ◽  
Ömer Şimşek
Keyword(s):  
Lactobacillus Plantarum ◽  
Immune Modulation ◽  
Radical Scavenging ◽  
Fermentation Medium ◽  
Potential Health ◽  
Tnf Α ◽  
Medium Level ◽  
Probiotic Strains ◽  
Health Promoting

The ability of Lactobacillus plantarum to produce exopolysaccharides (EPS) of various structures and properties is effective in showing both starter and probiotic culture qualification. In this study, the potential health promoting functions of the ropy EPS produced by Lactobacillus plantarum strains isolated from tarhana were tested. A stimulation of the pro-inflammatory IL-12 and TNF-α cytokines was observed in the presence of the ropy EPS suggesting an in vitro immune modulation. Similarly, the tested EPS demonstrated promoted the growth of the probiotic strains in fermentation medium. A medium level of radical scavenging activities of ropy EPS was observed whereas the superoxide and hydroxyl scavenging activities were more effective. The ropy EPS also showed α-glucosidase inhibition and cholesterol removal characteristics depending on their concentration. These findings revealed the potential health-promoting functions of ropy EPS from L. plantarum strains and EPS from L. plantarum PFC311 and PFC310 strains demonstrated multiple health-improving effects that can be further evaluated in food and other industries.

In Vitro Characterization of Lactic Acid Bacteria Isolated from Bovine Milk as Potential Probiotic Strains to Prevent Bovine Mastitis

2018 ◽  
Vol 11 (1) ◽  
pp. 74-84 ◽  
Author(s):  
Matías S. Pellegrino ◽  
Ignacio D. Frola ◽  
Berardo Natanael ◽  
Dino Gobelli ◽  
María E.F. Nader-Macias ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
In Vitro Characterization ◽  
Probiotic Strains

scholarly journals The Characterization and Microbiological Evaluation of Probiotic Isolated from Bambara Groundnut

2021 ◽  
Vol 9 (1) ◽  
pp. 54-64
Author(s):  
Babatunde Kazeem Adeoye ◽  
Elizabeth Funmilayo Aransiola ◽  
Gbenga Alebiowu ◽  
Mary Adejumoke Bisi-Johnson ◽  
Felix Oluwasola Olorunmola ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Lactobacillus Brevis ◽  
Bambara Groundnut ◽  
Lactobacillus Delbrueckii ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Enteropathogenic Bacteria ◽  
Antimicrobial Sensitivity ◽  
Freeze Dried

The study was carried out to isolate, characterize, and study antimicrobial sensitivity of lactic acid bacteria (LAB) from Bambara groundnut. Dried Bambara groundnut was fermented by spontaneous method for seven days and its pH, TTA (Total Titratable acidity) and microbial load monitored for each of the fermentation days. Seven acid-producing cultures were isolated from the sample, and isolates were further classified first by phenotype. Phenotypic and biochemical characteristics led to identification of three bacterial groups. These were followed by in vitro assessment of antimicrobial activity against enteropathogenic bacteria. The most abundant type of LAB distributed in the isolates of fermented Bambara groundnut was Lactobacillus delbrueckii, followed by Lactobacillus casei in two of the isolates. Lactobacillus brevis was found in the remaining two isolates. The growth pattern at different salt concentrations revealed that the isolates were salt tolerant at 2% and 4% while at 6.5% there was no growth. At pH 4.5 and 6.0, there were also growth. The strain evaluated showed in vitro antibacterial activity against five pathogenic microorganisms namely Escherichia coli, Salmonella sp, Shigella sp, Pseudomonas sp and Staphylococcus sp using agar well diffusion method. These results suggested that various LAB were present in Bambara groundnut. The microorganisms isolated were then freeze dried using a freeze drier and then kept at a low temperature in the refrigerator so as to preserve/store the organisms for further processes. This report thereby showed that Bambara groundnut, being an underutilized legume can serve as potential candidate for probiotic neutraceuticals. Int. J. Appl. Sci. Biotechnol. Vol 9(1): 54-64

scholarly journals Inhibitory Potential Of Lactobacillus Plantarum on Escherichia Coli

2017 ◽  
Vol 74 (2) ◽  
pp. 99 ◽  
Author(s):  
Laura MITREA ◽  
Lavinia Florina CĂLINOIU ◽  
Gabriela PRECUP ◽  
Maria BINDEA ◽  
Bogdan RUSU ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Plantarum ◽  
Alternative Therapy ◽  
Optimal Parameters ◽  
E Coli ◽  
Enteropathogenic Bacteria ◽  
Probiotic Strains ◽  
Inhibitory Potential ◽  
Mrs Broth

An alternative therapy to diarrhoeal episodes induced by E. coli is represented by probiotic strains (e.g Lactobacillus), which are able to maintain the gut micro-flora in optimal parameters. This work highlights the inhibitory potential of probiotics (L. plantarum ATCC 8014) over the enteropathogenic bacteria like E. coli ATCC 25922. For this study we co-incubated L. plantarum with E. coli in MRS broth at different concentrations (109, 108 CFU/mL – L. plantarum with 105, 104 CFU/mL – E. coli, 4 groups) for 24, 48 and 72h at 37oC. After co-incubation, E. coli colonies developed on Levine media were counted towards the control. E. coli colonies decreased after 48 and 72h of co-incubation with L. plantarum. Also, was noticed that L. plantarum (108 CFU/mL) completely inhibits E. coli (104 CFU/mL) after 72h of co-incubation. This work proves the inhibitory potential of probiotic strains (L. plantarum ATCC 8014) against enteropathogenic bacteria (E. coli ATCC 25922).

scholarly journals In Vitro Characterization of Lactobacillus plantarum Strains with Inhibitory Activity on Enteropathogens for Use as Potential Animal Probiotics

2017 ◽  
Vol 57 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Sasikumar Arunachalam Palaniyandi ◽  
Karthiyaini Damodharan ◽  
Joo-Won Suh ◽  
Seung Hwan Yang
Keyword(s):  
Lactobacillus Plantarum ◽  
Inhibitory Activity ◽  
In Vitro Characterization

Characterization and screening for probiotic potential of Lactic Acid Bacteria Isolated from Traditionally Fermented Wheat "Hamoum"

2018 ◽  
Vol 7 (4) ◽  
pp. 181-190
Author(s):  
Hafida Tahlaïti ◽  
Fatiha Dalache ◽  
Abdelkader Homrani ◽  
Saïd Nemmiche
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Brevis ◽  
Probiotic Properties ◽  
High Antibacterial Activity ◽  
Probiotic Potential ◽  
Starting Point ◽  
Probiotic Strains ◽  
One Year

The Algerian couscous, "Hamoum”, is prepared from fermented wheat which is the result of wheat grains being stored in an artisanal underground grana-ry called "Matmora" for more than one year. This method of storage has been abandoned in favor of conventional modes without being well studied and known. However, it is used on family scale farms. The purpose of this study was to explore the biodiversity of microbial flora of fermented wheat due to this type of storage. A total of 39 isolates were evaluated in terms to identify lactic acid bacteria and screening the probiotic properties. The phe-notypic results showed a diversity of lactic flora and lactic acid bacteria iso-lated from fermented wheat mainly belonging to Lactobacillus (69%) fol-lowed by Pediococcus (15%), Leuconostoc (8%) and Enterococcus (8%). The genotypic characterization revealed the dominance of Lactobacillus plantarum. The identified strains Lactobacillus plantarum (M6), Lactobacillus plantarum (R27), Lactobacillus brevis (BL8) and Pediococcus acidilactici (M5) have a high antibacterial activity and all strains have resistance to low acidic pH and bile salts. This investigation has enabled a screening of lactic acid bacteria from traditionally fermented wheat. “Hamoum” is a substrate rich in lactic acid bacteria that has exceptional probiotic potential. It’s may be the starting point for further characterization of biotechnological properties in order to identify and select probiotic strains for therapeutic purposes.

scholarly journals In Vitro and In Vivo Survival and Transit Tolerance of Potentially Probiotic Strains Carried by Artichokes in the Gastrointestinal Tract

2006 ◽  
Vol 72 (4) ◽  
pp. 3042-3045 ◽  
Author(s):  
Francesca Valerio ◽  
Palmira De Bellis ◽  
Stella Lisa Lonigro ◽  
Lorenzo Morelli ◽  
Angelo Visconti ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lactobacillus Plantarum ◽  
Bacterial Strains ◽  
Gastrointestinal Digestion ◽  
Feeding Study ◽  
Simulated Gastrointestinal Digestion ◽  
Probiotic Strains ◽  
Transit Tolerance

ABSTRACT The ability of potentially probiotic strains of Lactobacillus plantarum and Lactobacillus paracasei to survive on artichokes for at least 90 days was shown. The anchorage of bacterial strains to artichokes improved their survival in simulated gastrointestinal digestion. L. paracasei IMPC2.1 was further used in an artichoke human feeding study involving four volunteers, and it was shown that the organism could be recovered from stools.

Sign in / Sign up

Export Citation Format

Share Document