Isolation and Identification of Microorganisms Associated with Bioremediation of Oil Spilled Site in Bodo West, Rivers State, Nigeria.

2019 ◽  
pp. 1-8
Author(s):  
Tombari Bodo ◽  
Lekpa Kingdom David ◽  
Batombari Gbidum Gimah
Keyword(s):  
Aspergillus Niger ◽  
Local Government ◽  
Bacillus Anthracis ◽  
Micrococcus Luteus ◽  
Mineral Salt ◽  
Morphological Observation ◽  
Isolation And Identification ◽  
Streptococcus Faecalis ◽  
Rivers State

The samples collected from an oil spilled sites in Bodo West in Gokana Local Government of Rivers State in Nigeria were isolated to identify microorganisms associated with bioremediation. The population of about 311 different forming colonies were recorded in the study area; out of which 18 distinctive colonies were identified based on their morphological observation. From the selected isolates, 10 of them were assumed to be degraders because they form maximum clear zones on the mineral salt media. The results of the analysis show that notable number of microorganism of which seven bacteria and seven fungi were isolated and identified. The bacteria are Micrococcus luteus, Streptococcus lactic, Streptococcus epidemidis, Streptococcus faecalis, Clostridium sprogenes, Aerococcus viridems, and Bacillus anthracis. The fungi are Articulosspara inflate, Dendospora erecta, Aspergillus niger, Liodioderium Species, Geotichrum albdum, Aspergillus funigatus and Sreptothric atrax. On the strength of the result, it is inferred that microorganisms are associated with bioremediation and can be used for environmental and petroleum cleanup exercise in an oil spilled site.

scholarly journals Prevalence of Soil Transmitted Helminthes (STHs)Among Pupils of Community Primary Schools in Nkpor and Mgbodohia, Obio/Akpor Local Government Area, Rivers State, Nigeria

2018 ◽  
Author(s):  
LeBari Barine Gboeloh ◽  
Ike-Ihunwo Chikaire Ndamzi
Keyword(s):  
Public Health ◽  
Local Government ◽  
Mixed Infection ◽  
Primary Schools ◽  
Local Government Area ◽  
Sub Saharan Africa ◽  
Health Concern ◽  
Public Health Concern ◽  
Significant Difference ◽  
Rivers State

ABSTRACTSoil transmitted helminthes (STHs) are common public health concern among children in Sub saharan Africa. A study to determine the prevalence and intensity of these parasites among pupils of two primary schools in Nkpor and Mgbodohia communities, Obio/Akpor Local Government Area, Rivers State, Nigeria was conducted. The formo-ether concentration technique was used to concentrate and separate the eggs and cysts from the faeces. Out of 107 pupils (56males and 51 females) investigated, 81 (75.7 %) were positive for at least one gastrointestinal helminth. The parasites identified included Ascaris lumbricoide(58.0%), Hookworms(28.4%), Trichirus trichiura(13.6%) and A.lumbricoide + T. trichiura (6.3%). Ascaris lumbricoide was significantly (P<0.05) higher in prevalence than other parasites. Although more females (54.3%) were infected than males (45.7%), there was no significance(P>0.05) difference in the prevalence in relation to sex. Of the 44 males infected, 24(54.5%), 5 (11.4%), 13(29.5%) and 2(4.5%) haboured A. lumbricoide, T. trichiura, Hookworms and mixed infection (A. lumbricoide + T. trichiura) repectively. Out of the 37 females infected, 21 (56.8%), 3(8.1%), 10(27.0%) and 3(8.1%) haboured A. lumbricoide, T. trichiura, Hookworms and mixed infection (A. lumbricoide + T. trichiura) repectively. There was no significance difference in the prevalence of A. lumbricoide and Hookworms between males (54.5%) and females (56.8%). There was significant difference in prevalence among two major age groups (5-10years-45% and 11-15years-41.9%). Children within the age group of 16-20years had the least infection (9.9%). The intensities of A.lumbricoide, T.trichiura and hookworm were 246.5, 107.5 and 187 Epg respectively. The intensity of A. lumbricoide was significantly difference than other parasites identified.Soil transmited helminthes remain a public health concern among children in the study area. Provision of portable water, toilet facilities, good education on the epidemiology of STHs and regular de-worming will enhance control measures.

scholarly journals Survey of Technologies Used in Cassava Processing in Ikwerre Local Government Area of Rivers State

2020 ◽  
pp. 26-34
Author(s):  
N. S. Amadi ◽  
U. E. Solomon
Keyword(s):  
Local Government ◽  
Social Science ◽  
Local Government Area ◽  
Rivers State ◽  
Structured Questionnaire

The purpose of this study was to survey the technologies used in cassava processing in Ikwerre Local Government Area of Rivers State. Specifically, the study was proposed to determine the technologies used in harvesting, peeling, grinding, extracting water, sifting and frying crushed cassava tubers into Garri. A structured questionnaire titled “Survey of technologies used in cassava processing in Ikwerre Local Government Area of Rivers State (STUCP)”.was administered to one hundred-fifty farmers in ten villages in Ikwerre Local Government Area of Rivers State, Nigeria. Data was analyzed with the use of pictorial view of the technology used in cassava processing and Statistical package for social science was used in determining the frequency, percentage and mean. The findings revealed that more need to be done in terms of the technology used in harvesting, peeling, grinding, extracting water, sifting and frying crushed cassava tubers into Garri. One of the recommendations was that technology used in cassava processing should be improved in order to boost garri production in Rivers State.

scholarly journals Fungal Population and Physicochemical Characteristics of Abattoir-Impacted Soil in Iwofe, Rivers State

2021 ◽  
pp. 36-44
Author(s):  
Williams, Janet Olufunmilayo ◽  
Owhorji, Gloria
Keyword(s):  
Heavy Metals ◽  
Aspergillus Niger ◽  
Physicochemical Characteristics ◽  
Control Soil ◽  
Fungal Population ◽  
Candida Sp ◽  
Fungal Isolates ◽  
Fungal Load ◽  
Microbiological Techniques ◽  
Rivers State

Aim: To determine the fungal population and physicochemistry of abattoir impacted soil in Iwofe, Rivers State. Study Design: This study focused on Abattoir impacted soil. Statistical analysis of data and interpretation was carried out. Place and Duration of Study: Abattoir impacted soil was collected from three points in an abattoir located in Iwofe, Rivers State while the unpolluted soil which served as control was collected from the Rivers State University, Port Harcourt in January, 2021. Methodology: Standard microbiological techniques were used: the fungal population was determined by inoculating aliquots of an appropriate dilution resulting from a ten-fold serial dilution on prepared Sabouraud dextrose agar plates in duplicates. Plates were later incubated for 3-5 days after which colonies were enumerated and used in obtaining the fungal population in the soil samples while distinct colonies were subcultured for macroscopic and microscopic identification of fungi. The physicochemical parameters and heavy metals were analyzed using standard methods. Results: Fungal load in the control and abattoir impacted soil were 1.09×105 and 3.9×104 CFU/g, respectively. The fungal load of the control soil was significantly higher (P˂0.05) than the abattoir impacted soil. The fungal isolates identified in the abattoir impacted soil were Microsporium sp, Aspergillus niger and Candida sp while Aspergillus niger, Aspergillus flavus, Fusarium sp, Penicillium sp, Mucor sp and Rhizopus sp were identified from the control soil. The pH, temperature, nitrate and phosphate of the abattoir soil were 6.7, 28.33℃, 27.83(mgKg-1) and 1055(mgKg-1), respectively. The concentrations of Cadmium, Iron and Lead in the abattoir Impacted soil and control soil were 0.81, 563.35 and 7.12 mgKg-1, 0.51, 582.0 and 3.18 mgKg-1, respectively. The physico chemistry and heavy metals in the abattoir soil were within acceptable limits. Discussion and Conclusion: The findings from this study showed that heavy metals in abattoir impacted soil had an impact in the fungal population which led to the isolation of only three fungal isolates belonging to Microsporium sp, Candida sp and Aspergillus niger. More so, despite the presence of heavy metals in the abattoir impacted soil, the metals were all within permissible limits. Thus, the abattoir impacted soil was not heavily polluted.

Evaluation of microorganisms associated with vended frozen fish in Ado Ekiti locality

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 21-28
Author(s):  
P.T. Olagbemide ◽  
F.C. Akharaiyi
Keyword(s):  
Fish Species ◽  
Microbial Contamination ◽  
Micrococcus Luteus ◽  
Alcaligenes Faecalis ◽  
Total Coliform ◽  
Clupea Harengus ◽  
Microbial Infection ◽  
Isolation And Identification ◽  
Fish Samples ◽  
Frozen Fish

Fishes are world widely consumed by all categories of works of life because of their richness in protein, readily available and affordable by all. The basic nutrient of protein in fish that is so important in man’s diet also attracted microorganisms for their growth and multiplication. Meanwhile, the association of microorganisms in fishes depend on the environment of culture and their proliferation due to inadequate storage facilities. The aim of this study was focused on the isolation and identification of microorganisms from four frozen fish species sold in the Ado Ekiti metropolis. Frozen fish samples of Scomber scombrus (Atlantic Mackerel), Clupea harengus (Atlantic herring), Urophycis tenuis (White hake or mud hake) and Trachurus trachurus (Atlantic horse mackerel) from two markets in Ado Ekiti were microbiologically analyzed for possible microbial contamination. On the fish samples, the total heterotrophic count (THC) was 3.5×104 – 5.6×104 CFU/g, total coliform count (TCC) was 2.4×104 – 5.1×104 CFU/g, total Salmonella/Shigella count (TSSC) was 1.3×104 – 3.5×104 CFU/g, total Vibrio count (TVC) was 1.1×104 – 2.3×104 CFU/g and total fungal count (TFC) was 1.3×103 – 2.3×103 Spore/g were analyzed by cultural methods. There were variations in microbial loads among the fish species in the surveyed markets. The microorganisms identified with their percentage occurrence were Bacillus cereus (11.54%), Streptococcus faecium (13.46%), Alcaligenes faecalis (5.77%), Salmonella enterica serovar Typhi (5.77%), Micrococcus luteus (9.62%), Vibrio cholerae (7.69%), Aerococcus viridans (3.85%), Pseudomonas aeruginosa (7.69%), Xanthomonas fragariae (7.69%), Staphylococcus aureus (11.54%), Clostridium butyricum (7.68%), Escherichia coli (7.69%), Aspergillus fumigatus (11.11%), Aspergillus flavus (24.44%), Aspergillus clavatus (8.89%), Aspergillus fishcheri (6.69%), Aspergillus terreus (8.89%), Mucor mucedo (17.78%), Penicillium digitatum (13.33%) and Aspergillus parasiticus (8.89%). The results emphasized the microbial contamination of the fishes. The results obtained could serve as an awareness to consumers that microbial infection is possible from frozen fishes and as data for future reference in epidemiology or outbreak of disease from eating frozen fish.

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