scholarly journals A Comparative Study on Anti-inflammatory Activity of Hydroethanolic of Leaf, Stem and Root Extracts of Tecoma stans

2021 ◽  
pp. 214-221
Author(s):  
. Prateek ◽  
R. Gayatri Devi ◽  
J. Selvaraj ◽  
A. Jothi Priya
Keyword(s):  
Statistical Analysis ◽  
Comparative Study ◽  
Protein Denaturation ◽  
Biological Response ◽  
Biologically Active ◽  
Inflammatory Activity ◽  
Root Extracts ◽  
Anti Inflammatory ◽  
Range Test

Background: Tecoma stans is an important medicinal plant. The major bioactive compounds like alkaloids, phenols, terpenoids, glycosides, flavonoids and saponins had been isolated from this plant. The leaves bark and roots contain biologically active chemicals, and extracts from those tissues are in use as traditional folk medicines. Inflammation is a biological response of the immune system that can be triggered by a variety of factors, including pathogens, damaged cells and toxic compounds. The aim of the study was to analyze the anti-inflammatory activity of hydroethanolic leaf, stem and root extracts of Tecoma stans. Materials and methods: All chemicals and reagents were bought by sigma Chemicals Company. Anti inflammatory activity of the three different parts (leaf, stem and root) the plant were analysed by an in-vitro protein denaturation inhibition assays and inhibition calculation on protein inhibition percentage was done by the formula % inhibition=100−(A1 -A2)/A0 )*100). Statistical analysis was done by ANOVA and Duncan's multiple range test. Results: After comparing the anti-inflammatory activity of the leaf root stem of Tecoma stans, the results were plotted in graphs through statistical analysis using ANOVA and Duncan's multiple range test. The results showed that the stem of the plant has maximum anti-inflammatory activity compared to leaf and root. Conclusion: A comparative study of the extract or stem, leaf and root shows that the stem portion of the plant contains maximum anti-inflammatory activity.

scholarly journals Synthesis, Characterization and Evaluation of Anti-inflammatory Activity of Novel Indoline Derivatives

2016 ◽  
Vol 12 (12) ◽  
pp. 4557-4563
Author(s):  
Dhinesh kumar Manoharan
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Potent Inhibition ◽  
Ft Ir ◽  
Anti Inflammatory

Series of indoline derivatives were synthesized using N-(4-aminophenyl) indoline-1-carbothiamide as a precursor. The structures of synthesized compounds were confirmed by   FT-IR, 1H-NMR, 13C-NMR and LC-MS. The in vitro anti-inflammatory activity of synthesized indoline derivatives were examined by standard anti-denaturation assay. The compounds 4a (IC50 = 62.2 µg/ml) and 4b (IC50 = 60.7 µg/ml) showed potent inhibition on protein denaturation. The compounds 5a (IC50 = 97.8 µg/ml) exhibits moderate inhibition on protein denaturation

scholarly journals Anti-inflammatory activity of Punica granatum extract on oral microbes - In vitro

2019 ◽  
Vol 10 (2) ◽  
pp. 1019-1022
Author(s):  
Westeros Dominic Pereira ◽  
Geetha RV ◽  
Lakshmi Thangavelu
Keyword(s):  
Protein Denaturation ◽  
Periodontal Diseases ◽  
Punica Granatum ◽  
Inflammatory Activity ◽  
Oral Diseases ◽  
Major Health Problem ◽  
Maximum Inhibition ◽  
Anti Inflammatory ◽  
Oral Microbes

To study the anti-inflammatory effect of Punica granatum extract against the oral microbes. Oral diseases continue to be a major health problem worldwide. Dental caries and periodontal diseases are among the most important global oral health problems, although conditions such as oral and pharyngeal cancers and oral tissue lesions are also significant health concerns. Pomegranate extracts have been used for centuries in traditional medicine to confer health benefits in a number of inflammatory diseases, microbial infections and cancer. The anti-inflammatory activity of pomegranate extract was evaluated by protein denaturation assay, and the results were read spectrophotometrically. Denaturation of proteins is a great‐ documented cause of inflammation. As a part of the investigation on the mechanism of the anti-inflammatory activity, the ability to extract to inhibit protein denaturation was studied. It was effective in inhibiting heat induced albumin denaturation at different concentrations as shown in Table 1. Maximum inhibition, 70.12±1.12% was observed at500µg/ml. IC50 value was found to be 105.35±1.99µg/ml. Aspirin, a standard anti-inflammatory drug showed the maximum inhibition, 77.12±1.42% at the concentration of 200µg/ml. Hence it can be concluded that pomegranate extract has anti-inflammatory property and also can be used in products such as toothpaste and mouth wash etc.

scholarly journals In vitro Evaluation of Anti-Inflammatory Activity of Symplocos racemosa Using Protein Denaturation Assay

2021 ◽  
pp. 715-720
Author(s):  
K. Janani ◽  
R. V. Geetha ◽  
S. Rajeshkumar
Keyword(s):  
Plant Extract ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Whatman Filter Paper ◽  
Traditional Drug ◽  
Herbal Plants ◽  
Anti Inflammatory ◽  
Herbal Formulations ◽  
Symplocos Racemosa

Introduction: Recently there is considerable awareness and interest in the field of herbal medicine due to its natural origin and lesser side effects compared to Allopathy. Selected herbal plants like Symplocos racemosa, commonly known as lodhra, are found mainly in plains and lower hills of Bengal. The word ‘Lodhra’ means ‘Propitious’. Symplocos racemosa is an important Indian traditional drug used in many Ayurvedic and herbal formulations for treatment of liver as well as uterine disorders and leucorrhea. Ethnobotanical Literature indicates use of Symplocos racemosa in treatment of eye disease, skin disease, ear disorders, liver and bowel complaints, tumours,uterine disorders, spongy and bleeding gums, asthma, fever, snakebite, gonorrhoea and arthritis. Aim: To analyse the anti-inflammatory activity of Symplocos racemosa using protein denaturation assay. Materials and Methods: 2 g of Lodhra bark powder is mixed with 100 ml distilled water & boiled for 20 min at 50°C. The extract is filtered using whatman filter paper & concentrated to 10 ml.1 ml each of Bovine serum albumin is added to various fixations of plant extract (10μL - 50 μL) and the anti - inflammatory activity was evaluated by analysing the percentage inhibition. Results: From this study, it is evident that Lodhra has significant anti-inflammatory activity. At 50μℓ concentration, the plant extract shower higher anti- inflammatory activity of 76%. Conclusion: Symplocos racemosa extract has proved to exhibit effective anti- inflammatory activity. Further studies have to be carried to analyse the other properties of this herb, which can be incorporated successfully in the pharmaceutical industry.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF NIEBUHRIA APETALA (ROTH) DUNN – IN VITRO MODELS

2019 ◽  
pp. 278-281
Author(s):  
RAJESH A ◽  
DOSS A ◽  
TRESINA PS ◽  
MOHAN VR
Keyword(s):  
Methanol Extract ◽  
Protein Denaturation ◽  
In Vitro Models ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Inflammatory Agent ◽  
Potential Source ◽  
Anti Inflammatory

Objective: The objective of this study was to determine the anti-inflammatory activity of methanol extract of Niebuhria apetala and its possible mechanism of action. Methods: Methanol extract of Niebuhria apetala leaf (NAL) was assessed for its anti-inflammatory activity by in vitro methods. Using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and antilipoxygenase activity at different concentrations, in vitro anti-inflammatory activity was estimated. The standard drug used for this purpose was aspirin. Results: Methanol extract NAL at a concentration range of 100–500 μg/ml significant (p<0.01) protects the heat-induced protein denaturation. At the concentration of 500 mg/ml, NAL showed significant (p<0.01) inhibition of protease inhibitory action. Heat-induced hemolysis of erythrocyte, hypotonicity-induced hemolysis, and lipooxygenase activity were significant (p<0.01) inhibited at the concentration of 500 μg/ml. Conclusion: Finally, the present study indicates that methanol extract of Niebuhria apetala can be a potential source of anti-inflammatory agent.

scholarly journals FORMULATION AND EVALUATION OF TOPICAL HERBAL GEL CONTAINING INCLUSION COMPLEX OF CURCUMIN

2019 ◽  
pp. 196-201
Author(s):  
VANDANA D ◽  
SHWETA PAWAR
Keyword(s):  
Inclusion Complex ◽  
Protein Denaturation ◽  
Release Kinetics ◽  
Inflammatory Activity ◽  
In Vitro Dissolution ◽  
Diffusion Method ◽  
Topical Gel ◽  
In Vitro Diffusion ◽  
Anti Inflammatory

Objective: The current work was aimed to prepare a topical gel containing curcumin (CUR) for the treatment of microbial infections on skin. Methods: CUR was complexed with the β-cyclodextrin (β-CD) using kneading method in 1:1, 1:2, and 1:3 molar ratios and characterized. The inclusion complex with high aqueous solubility was loaded in the topical gel containing (2% CUR) which was prepared using carbopol, sodium CMC, and guar gum and evaluated for viscosity, spreadability, extrudability, pH, drug content, and in vitro diffusion studies. The in vitro anti-inflammatory activity of the gel was performed by albumin protein denaturation technique, the statistical analysis was done using ANOVA followed Dunnett’s t-test. The antimicrobial activity of CUR was evaluated using standard strains of Candida albicans and Escherichia coli by agar well diffusion method. Results: The complexation of CUR had an increased solubility up to 103.09 times for 1:3 molar ratio with in vitro dissolution 90.64% for 60 min. The optimized formulation F9 had viscosity of 6500.3 cps and 97.5% in vitro drug diffusion for 8 h which follows zero-order release kinetics. In vitro anti-inflammatory activity studied showed that the CUR gel has a good potency for renaturation and was as effective as standard diclofenac with 76.9% inhibition (p=0.0507). CUR showed approx. 3 mm diameter of zone of inhibition against C. albicans and E. coli. Conclusion: A stable topical gel of CUR using β-CD and carbopol was successfully prepared which showed better in vitro diffusion with promising anti-inflammatory and antifungal action.

scholarly journals Anti-inflammatory and Anti-arthritic Potentials of Maesa Montana Roots

2021 ◽  
Vol 18 (16) ◽  
Author(s):  
Sanjida SHARMIN ◽  
Rabeya Gazi JHUMA ◽  
Sanjida ISLAM ◽  
Riniara KHATUN
Keyword(s):  
Bovine Serum ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Flowering Plant ◽  
Activity Measurement ◽  
Root Extract ◽  
Standard Drug ◽  
Activity Test ◽  
Anti Inflammatory

Maesa Montana is a flowering plant of the Myrsinaceae family, which is locally known as ramjani. The current study aimed to evaluate the in-vitro anti-arthritic and anti-inflammatory activities of the root extract of this plant. The anti-inflammatory performances were measured by the hindrance of egg white denaturation, and anti-arthritic activity was investigated by Bovine serum protein. In the anti-inflammatory activity test, the methanolic root extract of this plant showed 69.29 ± 1.19 % of inhibition at a concentration of 1000 µg/mL and standard drug exhibited 90.11 ± 1.45 % of inhibition at the same concentration. Furthermore, in the anti-arthritic activity test, the extract demonstrated 68.18 ± 1.34 % of inhibition at a concentration of 1000 µg/mL whereas the standard diclofenac drug showed 90.65 ± 1.19 % of inhibition at the same concentration. These results revealed that the root extract of this plant possesses significant anti-arthritic and anti-inflammatory activities based on the inhibition of BSA and protein denaturation. HIGHLIGHTS Methanol soluble compounds extraction from the roots of Maesa Montana Anti-inflammatory activity determination using egg albumin Anti-arthritic activity measurement using bovine serum albumin GRAPHICAL ABSTRACT

scholarly journals In vitro antioxidant anti inflammatory and cytotoxicity activities from Hexane extract of Bryonopsis laciniosa fruits.

2018 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Sanjeevkumar C.B ◽  
Ramesh L Londonkar ◽  
Umesh Madire Kattegouda
Keyword(s):  
Cell Line ◽  
Potential Application ◽  
Protein Denaturation ◽  
Cancer Cell Line ◽  
Hexane Extract ◽  
Inflammatory Activity ◽  
Cytotoxicity Activity ◽  
Anti Inflammatory ◽  
Mcf 7

<p><em>Bryonopsis laciniosa </em>also known as “Shivlingi” annual climber with bright red fruits and is reported to be highly medicinal in India<em>. </em>As a folk medicine, the plant is used in treatment of broad range of diseases and disorders. In the present study, Hexane extract of <em>B. laciniosa </em>fruits were used to evaluate <em>in</em> <em>vitro</em> anti inflammatory, antioxidant and Cytotoxicity (towards MCF-7 cell line) activities.<em> In vitro</em> anti inflammatory activity by inhibition of protein denaturation, antioxidant assays like DPPH, ABTS, H<sub>2</sub>O<sub>2</sub> and FRAP were used to measure the antioxidant capacity of the hexane extracts and cytotoxicity activity using MCF-7 breast cancer cell line. Hexane extract showed the effective antioxidant activity in all assays compared to ascorbic acid and BHT. The results for<em> In vitro</em> anti inflammatory activity of hexane extract and Dichlofenac drug were equivalent, hexane extract showed promising activity for inhibition of protein denaturation assay. The cytotoxicity activity from hexane extract was noticeable against MCF-7 cell line. The overall results show potential application of <em>Bryonopsis laciniosa </em>fruits suggesting their potential application as a health-promoting functional ingredient or natural preservative in foods.</p>

scholarly journals In vitro anti-inflammatory activity of mangrove plant Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae)

2018 ◽  
Vol 5 (10) ◽  
pp. 417-426 ◽  
Author(s):  
Samanjit Kaur ◽  
M. Syed Ali ◽  
V. Anuradha ◽  
V. Suganya ◽  
A. Ashashalini ◽  
...  
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Root Extract ◽  
Rhizophora Mucronata ◽  
Membrane Stabilization ◽  
In Vivo Models ◽  
Maximum Inhibition ◽  
Anti Inflammatory

In the present study, analysis of in vitro inflammatory showed whole plant of Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae) can be the potent source. The data from this study showed that the R. mucronata leaf, bark and root extract could serve as an important anti-inflammatory agent. Moreover, among the three extracts, the stilt root and leaves extract showed highest anti inflammatory. In vitro anti-inflammatory activity of the selected plant extracts was evaluated using albumin denaturation, membrane stabilization and proteinase inhibitory assays. As part of the investigation on the mechanism of the anti-inflammation activity, ability of extract protein denaturation was studied. Maximum inhibition (296.26%) was observed from root extract followed by bark (259.48%) and leaf (237.62%). The extracts inhibited the heat induced hemolysis of RBCs to varying degree as show in table below. The maximum inhibition 284.17% was observed from bark extract followed by root (265.05%) and leaf (232.61%). It reveals that these phytochemical constituents are responsible to maximum protection of protein denaturation, albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory and anti-arthritic activities by in vivo models.

scholarly journals In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Puneet Singh ◽  
Yogesh Sharma ◽  
Ashutosh Sharma
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Local Market ◽  
Plant Materials ◽  
Whole Plant ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory ◽  
Convolvulus Pluricaulis

Aim: In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy. Material & Methods- The whole plant parts of Convolvulus pluricaulis Choisy were purchased from the local market. Whole plant materials were dried under shade and subjected to coarse powder for extraction process. Accurately weighed quantity of whole plant material was extracted using 95 % methanol by soxhlet apparatus for 72 h. Qualitative chemical tests of methanolic extracts were subjected to various chemical tests to detect various phytoconstituents. Solvent systems ethyl acetate: methanol: water (77:13:10) were found to be most satisfactory solvent system. After development of plates, they were air-dried and number of spots, color and Rf values were recorded. The % heamolysis was calculated by assuming the heamolysis produced by the control group as 100 %. Results: The preliminary phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds. At a concentration of 500 µg/ml, the extract produced 71.59% protection of RBC haemolysis as compared with 72.73% produced by prednisolone.  The methanolic extract of selected plant showed 39.70% inhibition. The Diclofenac sodium showed 55.88 % inhibition against denaturation of protein. Conclusion: In conclusion, it can be stated that the methanolic extract has beneficial effects in long lasting in membrane stabilizing method, inhibition of protein denaturation method and proteinase model. Keywords: In-Vitro, Anti-inflammatory Activity, Methanolic Extract, Convolvulus pluricaulis Choisy, Protein Denaturation Method

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