scholarly journals Effects of Water Treatment Methods on Protein Profile of Escherichia coli O157:H7 and Escherichia coli Non-O157 Isolated from Drinking Water Sources in Ado-Ekiti, Ekiti State, Nigeria

2017 ◽  
Vol 20 (5) ◽  
pp. 1-10
Author(s):  
Busayo Olowe ◽  
Jacob Oluyege ◽  
Oladiran Famurewa ◽  
Titilayo Femi-Ola ◽  
Adebayo Osesusi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Treatment ◽  
Protein Profile ◽  
Water Sources ◽  
Escherichia Coli O157 ◽  
Treatment Methods ◽  
Drinking Water Sources

scholarly journals Prevalence of Antibiotic-Resistant Escherichia coli in Drinking Water Sources in Hangzhou City

2017 ◽  
Vol 8 ◽  
Author(s):  
Zhaojun Chen ◽  
Daojun Yu ◽  
Songzhe He ◽  
Hui Ye ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Sources ◽  
Antibiotic Resistant ◽  
Drinking Water Sources

Occurrence and separation of Cryptosporidium oocysts in drinking water treatment

2000 ◽  
Vol 41 (7) ◽  
pp. 159-163 ◽  
Author(s):  
P. Dolejš ◽  
O. Ditrich ◽  
T. Machula ◽  
N. Kalousková ◽  
G. Puzová
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Removal Efficiency ◽  
Drinking Water Treatment ◽  
Water Sources ◽  
Mixing Conditions ◽  
Drinking Water Sources ◽  
Cryptosporidium Oocysts ◽  
Mixing Intensity ◽  
Treatment Train

The presence of Cryptosporidium sp. in Czech drinking water sources was monitored. High numbers of Cryptosporidium oocysts were found in the Slezska Harta reservoir after 1997 summer floods. The influence of iron (III) coagulant dose, mixing conditions and preozonation on the removal efficiency of Cryptosporidium oocysts was studied. Low mixing intensity produced flocs with poor settling features. The efficiency of sedimentation expressed as residual iron after sedimentation was proportional to removal efficiency of oocysts. Both underdosing or overdosing of iron (III) coagulant resulted in decrease of COD, turbidity and oocyst removal. Preozonation positively influenced removal efficiency of oocysts in the conventional drinking water treatment train studied.

Contamination of Canadian private drinking water sources with antimicrobial resistant Escherichia coli

2013 ◽  
Vol 47 (9) ◽  
pp. 3026-3036 ◽  
Author(s):  
Brenda L. Coleman ◽  
Marie Louie ◽  
Marina I. Salvadori ◽  
Scott A. McEwen ◽  
Norman Neumann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Sources ◽  
Drinking Water Sources

scholarly journals Abundance of pathogenic Escherichia coli, Salmonella typhimurium and Vibrio cholerae in Nkonkobe drinking water sources

2006 ◽  
Vol 4 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Maggy N. B. Momba ◽  
Veronica K. Malakate ◽  
Jacques Theron
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Salmonella Typhimurium ◽  
Vibrio Cholerae ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Water Sources ◽  
E Coli ◽  
Drinking Water Sources

In order to study the prevalence of enteric pathogens capable of causing infection and disease in the rural communities of Nkonkobe, bacterial isolates were collected from several surface water and groundwater sources used by the community for their daily water needs. By making use of selective culture media and the 20E API kit, presumptive Escherichia coli, Salmonella spp. and Vibrio cholerae isolates were obtained and then analysed by polymerase chain reaction assays (PCR). The PCR successfully amplified from water samples a fragment of E. coli uidA gene that codes for β-D-glucuronidase which is a highly specific characteristic of enteropathogenic E. coli, enterotoxigenic E. coli and entero-invasive E. coli. The PCR also amplified the epsM gene from water samples containing toxigenic V. cholerae. Although E. coli was mostly detected in groundwater sources, toxigenic V. cholerae was detected in both surface and groundwater sources. There was a possibility of Salmonella typhimurium in Ngqele and Dyamala borehole water samples. The presence of these pathogenic bacteria in the above drinking water sources may pose a serious health risk to consumers.

scholarly journals Detection of RpoS Gene in Escherichia Coli O157:H7 and non-O157 and Their Survival Pattern in Water Treatment Methods

2020 ◽  
Vol 1 (1) ◽  
pp. 14-21
Author(s):  
lowe, B. M. ◽  
Adelegan, O.
Keyword(s):  
Escherichia Coli ◽  
Water Treatment ◽  
Moringa Oleifera ◽  
Escherichia Coli O157 ◽  
High Concentration ◽  
Treatment Methods ◽  
E Coli ◽  
Rpos Gene ◽  
Test Organisms ◽  
Survival Pattern

This study aimed to detect presence of RpoS gene in Escherichia coli O157:H7 and non-O157 and investigate their survival pattern in different water treatment methods. A total of fifteen serologically and molecularly identified E. coli was selected from a previous work, out of which eight were Escherichia coli O157 and seven were E. coli non-O157. From among these, S30 and S89 identified isolates served as presentative E. coli O157:H7 and non-O157 respectively for survival studies. The water treatment methods used employed included: use of silver, lime, storage, acidification (low pH), high temperature and Moringa oleifera. Survival pattern of the test organisms under the influence of these methods were carried out using standard techniques. Molecular detection of stress response gene, RpoS, in the fifteen (15) test organisms was performed following manufacturer’s instruction. Results showed that for both test organisms, silver was bactericidal at high concentration while storage allows their survival up till 21 days though with a reduction in cfu. Both organisms showed low survival at pH 9 while E. coli O157:H7 and non-O157 could survive at pH 4 and 6 respectively. E. coli O157:H7 survived better than non-O157 at high concentration of lime. While both survived at low temperature, E. coli O157:H7 survive better at 60oC. Sunlight and chlorine showed mild and complete bactericidal action respectively with increased exposure time for both test organisms. Moringa oleifera was only effective at a reduced concentration on the two organisms. Detection of RpoS genes showed that only 66.7% carried the gene in them while 33.3% did not. Findings from this study show that the possession of stressed genes in bacteria causing waterborne disease could allow these organisms to survive water treatment methods adopted in many under developed countries or rural communities. This suggests a threat to health of these communities.

scholarly journals Antimicrobial Resistance, Virulence Factors, and Pathotypes of Escherichia coli Isolated from Drinking Water Sources in Jordan

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 86 ◽  
Author(s):  
Samer Swedan ◽  
Heba Abu Alrub
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Sources ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Disk Diffusion Method ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Drinking Water Sources ◽  
Synergy Test

The study investigated the prevalence of potentially pathogenic and drug resistant Escherichia coli among drinking water sources in Jordan. A total of 109 confirmed E. coli isolates were analyzed. Antimicrobial susceptibility testing was done using the Kirby Bauer disk diffusion method. Phenotypic identification of extended spectrum beta-lactamase (ESBL) and carbapenemase production was done using the double disk synergy test and the modified Hodge test, respectively. Isolates’ plasmid profiles were determined by gel electrophoresis. PCR was used for detection of virulence and resistance genes. Overall, 22.0% of the isolates were potentially intestinal pathogenic E. coli (IPEC); namely enteroaggregative E. coli (16.5%), enteropathogenic E. coli (2.8%), enteroinvasive E. coli (1.8%), and enterohemorrhagic E. coli (0.9%). A third of the isolates were multi-drug resistant. The highest rates of antimicrobials resistance were observed against ampicillin (93.6%) and sulfamethoxazole/trimethoprim (41.3%). All isolates were susceptible to imipenem, meropenem, doripenem and tigecycline. The prevalence of ESBL and carbapenemase producers was 54.1% and 2.8%, respectively. BlaVIM was the most prevalent resistance gene (68.8%), followed by blaCTX (50.5%), blaTEM (45.9%), blaNDM (11%), blaKPC (4.6%), and blaSHV (0.9%). Fifty-eight (53.2%) isolates contained one or more plasmid ranging from 1.0 to 8.0 kbp. Overall, high prevalence of potentially pathogenic and resistant isolates was observed.

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