Isolation, Identification and Antibiotic Susceptibility of Escherichia coli Isolated from Raw Meat from Modake and Ile-Ife, Osun State, Nigeria

The study reported isolation, identification and antibiotic susceptibility of Escherichia coli isolated from raw meat from Modakeke and Ile-ife, Osun State, Nigeria, with the view to determining the antibiogram profiling of the bacterial isolates. In this study, five samples of fresh meat were collected from different abattoirs in Ile-Ife and Modakeke, Osun State. Isolates of Escherichia coli were isolated, identified morphologically based on their growth on nutrient agar and subjected to antibiotic susceptibility test on Mueller Hinton agar. The mean microbial load from the meat samples ranged from 8.85 x 102cfu/ml to 5.77 x 104cfu/ml. A total of 69 E. coli isolates were recovered from the meat sampled. All the isolates appeared cream, translucent, entire, convex, circular, smooth and glistering. The isolates were identified as Gram negative rods, non-motile, lactose fermenters, positive for indole test and negative for citrate utilization test. All the E. coli isolates were resistant to augmentin, ceftriazone, nitrofurantoin and gentamycin. 98.55% of E. coli isolated was resistant to amoxillin and the least resistant was recorded in ofloxacin (8.70%). However, 91.30% of the E. coli isolates was sensitive to ofloxacin, 81.16% to ciprofloxacin and 36.23% to pefloxacin while none was sensitive to augmentin, ceftriazone, nitrofurantoin and gentamycin. A total of 19 different multiple antibiotic resistance patterns were observed among the isolates. Thirty isolates (43.48%) showed multiple antibiotic resistance to 5 and 10 different antibiotic types each. The study concluded that occurrence of E. coli infection is high in the study area with high level of multiple antibiotic resistance.

Download Full-text

Antibiotic susceptibility pattern of Escherichia coli isolated from food, cooking utensils and palms of food handlers in some restaurants in Zaria, Nigeria

AROC in Pharmaceutical and Biotechnology ◽

10.53858/arocpb01022834 ◽

2021 ◽

Vol 1 (2) ◽

pp. 28-34

Author(s):

Victoria Etalong ◽

Babajide A. Tytler ◽

Igba Profit ◽

Adeshina O. Gbonjubola

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Antibiotic Susceptibility ◽

Resistance Index ◽

Major Public Health Problem ◽

Multiple Antibiotic Resistance ◽

Food Handlers ◽

Susceptibility Pattern ◽

Antibiotic Susceptibility Pattern ◽

E Coli

Foodborne disease is a major public health problem causing considerable morbidity and mortality annually. In the present study, the antibiotic susceptibility patterns of Escherichia coli isolated from food, cooking utensils and palms of food handlers in some restaurants in Zaria, Nigeria were evaluated. A total of 250 samples (220 food samples, 7 hand samples of food handlers, 10 plate samples within restaurants and 13 spoon samples) were collected from five locations in Zaria, Nigeria and analysed for microbial contaminations using standard microbiological techniques. The antibiotic susceptibility pattern of the isolates was determined using Kirby-Bauer modified disc agar diffusion technique. Results revealed that out of 158 acclaimed Enterobacteriaceae isolates evaluated, 19 % (30) were confirmed to be E. coli, while 81 % were Klebsiella. spp, Citrobacter fruendii, Enterobacter spp, Shigella spp, Salmonella spp, Serratia spp, and Cronobacter sakazaki. The majority of the isolates were resistant to amoxiclav (26.08%), ampicillin (26.08%), tetracycline (26.08%) and metronidazole (13.04%). A 33.3% of the isolates were multidrug-resistant. The E. coli isolates were mostly multiple antibiotic resistance with 43.3% having multiple antibiotic resistance index (MARI) ≥ 0.2. In conclusion, E. coli evolved resistance to ampicillin, Amoxicillin Clavulanic acid, and Tetracycline and other tested antimicrobial drugs which would make the treatment of Escherichia coli infections difficult

Download Full-text

Antibiotic susceptibility pattern of biofilm forming uropathogenic Escherichia coli isolated from UTI infected patients of Koshi Zonal Hospital in Biratnagar, Nepal

BIBECHANA ◽

10.3126/bibechana.v16i0.19192 ◽

2018 ◽

Vol 16 ◽

pp. 47-54

Author(s):

S Chaudhary ◽

B Khatiwada ◽

N K Chaudhary

Keyword(s):

Escherichia Coli ◽

Antibiotic Susceptibility ◽

Uropathogenic Escherichia Coli ◽

Urine Samples ◽

Resistance Pattern ◽

Isolation And Identification ◽

Antibiotic Susceptibility Pattern ◽

E Coli ◽

Diffusion Technique ◽

Antibiotic Susceptibility Test

Objectives: To investigate the prevalence and antibiotic resistance pattern of biofilm-forming Uropathogenic Escherichia coli (UPEC) from urine samples isolated from UTI infected patients of Koshi zonal hospital, Biratnagar.Methods: A total of 51 urine samples from urinary tract infected patients were collected from Koshi zonal hospital, Biratnagar in the period of July to August 2017. Following the isolation and identification of biofilm-forming uropathogenic Escherichia coli, antibiotic susceptibility test was performed by a modified Kirby-Bauer disc diffusion technique. The biofilm detection was done by Congo red agar method.Results: In the present study, 45% of the urine samples showed a predominant growth of E. coli, among which 70% of isolates exhibited positive biofilm formation. Biofilm forming isolates revealed 100%, 87.5%, 75%, 63% and 12.5% resistant to erythromycin, amoxicillin, cefotaxime, levofloxacin, and nitrofurantoin respectively. Approximately 87.5% of biofilm-forming isolates were found multi-drug resistant.Conclusion: The study revealed the major issue of UTI by E. coli which may be due to poor sanitation, not the proper cleanliness of genitals and unsafe sexual intercourse. Nitrofurantoin and levofloxacin were examined the most effective antibiotics for UPEC. BIBECHANA 16 (2019) 47-54

Download Full-text

Inhibition of the multiple antibiotic resistance (mar) operon in Escherichia coli by antisense DNA analogs.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.41.12.2699 ◽

1997 ◽

Vol 41 (12) ◽

pp. 2699-2704 ◽

Cited By ~ 31

Author(s):

D G White ◽

K Maneewannakul ◽

E von Hofe ◽

M Zillman ◽

W Eisenberg ◽

...

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Antisense Oligonucleotides ◽

Multiple Antibiotic Resistance ◽

Wild Type ◽

Lacz Expression ◽

E Coli ◽

Plasmid Construct ◽

Dna Analogs ◽

Susceptibility And Resistance

The multiple antibiotic resistance operon (marORAB) in Escherichia coli controls intrinsic susceptibility and resistance to multiple, structurally different antibiotics and other noxious agents. A plasmid construct with marA cloned in the antisense direction reduced LacZ expression from a constitutively expressed marA::lacZ translational fusion and inhibited the induced expression of LacZ in cells bearing the wild-type repressed fusion. The marA antisense construction also decreased the multiple antibiotic resistance of a Mar mutant. Two antisense phosphorothioate oligonucleotides, one targeted to marO and the other targeted to marA of the mar operon, introduced by heat shock or electroporation reduced LacZ expression in the strain having the marA::lacZ fusion. One antisense oligonucleotide, tested against a Mar mutant of E. coli ML308-225, increased the bactericidal activity of norfloxacin. These studies demonstrate the efficacy of exogenously delivered antisense oligonucleotides targeted to the marRAB operon in inhibiting expression of this chromosomal regulatory locus.

Download Full-text

Occurrence and Characterization of Escherichia coli O157 and Other Serotypes in Raw Meat Products in Morocco

Journal of Food Protection ◽

10.4315/0362-028x-71.10.2082 ◽

2008 ◽

Vol 71 (10) ◽

pp. 2082-2086 ◽

Cited By ~ 5

Author(s):

LUCIANO BENEDUCE ◽

GIUSEPPE SPANO ◽

ARI Q. NABI ◽

FRANCESCO LAMACCHIA ◽

SALVATORE MASSA ◽

...

Keyword(s):

Escherichia Coli ◽

Susceptibility Testing ◽

Meat Products ◽

Molecular Techniques ◽

Escherichia Coli O157 ◽

Raw Meat ◽

E Coli ◽

Meat Samples ◽

The City

In this study, 100 raw meat samples were collected from 15 local Moroccan butcheries in five different areas of the city of Rabat during a period of 4 months. Overall, 7 of 15 butcheries from three areas of the city yielded strains of Escherichia coli O157. Single isolates from 9 (9%) of 100 raw meat samples were biochemically and serologically confirmed as E. coli O157. Using molecular techniques, two strains were positive for the Shiga toxin, with two additional strains containing an attaching-effacing gene. All potentially virulent serotypes isolated from these meat samples showed distinct pulsed-field gel electrophoresis profiles. Based on antibiotic susceptibility testing, more than 70% of the isolates were resistant to ampicillin and clavulanic acid–amoxicillin. Moreover, one strain was resistant to more than three antibiotics. Our study represents the first survey of E. coli O157 and related serotypes in raw meat products in Morocco.

Download Full-text

Constitutive soxR Mutations Contribute to Multiple-Antibiotic Resistance in Clinical Escherichia coli Isolates

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.49.7.2746-2752.2005 ◽

2005 ◽

Vol 49 (7) ◽

pp. 2746-2752 ◽

Cited By ~ 63

Author(s):

Anastasia Koutsolioutsou ◽

Samuel Peña-Llopis ◽

Bruce Demple

Keyword(s):

Nitric Oxide ◽

Escherichia Coli ◽

Antibiotic Resistance ◽

Dna Sequences ◽

Single Point ◽

Constitutive Expression ◽

Clinical Isolates ◽

Multiple Antibiotic Resistance ◽

E Coli ◽

First Time

ABSTRACT The soxRS regulon of Escherichia coli and Salmonella enterica is induced by redox-cycling compounds or nitric oxide and provides resistance to superoxide-generating agents, macrophage-generated nitric oxide, antibiotics, and organic solvents. We have previously shown that constitutive expression of soxRS can contribute to quinolone resistance in clinically relevant S. enterica. In this work, we have carried out an analysis of the mechanism of constitutive soxS expression and its role in antibiotic resistance in E. coli clinical isolates. We show that constitutive soxS expression in three out of six strains was caused by single point mutations in the soxR gene. The mutant SoxR proteins contributed to the multiple-antibiotic resistance phenotypes of the clinical strains and were sufficient to confer multiple-antibiotic resistance in a fresh genetic background. In the other three clinical isolates, we observed, for the first time, that elevated soxS expression was not due to mutations in soxR. The mechanism of such increased soxS expression remains unclear. The same E. coli clinical isolates harbored polymorphic soxR and soxS DNA sequences, also seen for the first time.

Download Full-text

Isolation, identification, and antimicrobial susceptibility pattern of Campylobacter jejuni and Campylobacter coli from cattle, goat, and chicken meats in Mekelle, Ethiopia

PLoS ONE ◽

10.1371/journal.pone.0246755 ◽

2021 ◽

Vol 16 (2) ◽

pp. e0246755

Author(s):

Yohans Hagos ◽

Getachew Gugsa ◽

Nesibu Awol ◽

Meselu Ahmed ◽

Yisehak Tsegaye ◽

...

Keyword(s):

Campylobacter Jejuni ◽

Antibiotic Susceptibility ◽

Diffusion Method ◽

Campylobacter Coli ◽

Susceptibility Pattern ◽

Isolation And Identification ◽

Raw Meat ◽

Antibiotic Susceptibility Test ◽

Meat Samples ◽

Campylobacter Spp

Campylobacter jejuni and Campylobacter coli are globally recognized as a major cause of bacterial foodborne gastroenteritis. A cross-sectional study was conducted from October 2015 to May 2016 in Mekelle city to isolate, identify, and estimate the prevalence of C. jejuni and C. coli in raw meat samples and to determine their antibiotic susceptibility pattern. A total of 384 raw meat samples were randomly collected from bovine (n = 210), goat (n = 108), and chicken (n = 66), and isolation and identification of Campylobacter spp. were performed using standard bacteriological techniques and PCR. Antibiotic susceptibility test was performed using disc diffusion method. Of the total 384 raw meat samples, 64 (16.67%) were found positive for Campylobacter spp. The highest prevalence of Campylobacter spp. was found in chicken meat (43.93%) followed by bovine meat (11.90%) and goat meat (9.25%). The most prevalent Campylobacter spp. isolated from meat samples was C. jejuni (81.25%). The overall prevalence of Campylobacter in restaurants, butcher shops, and abattoir was 43.93%, 18.30%, and 9.30%, respectively. 96.8%, 81.25%, 75%, and 71% of the Campylobacter spp. isolates were sensitive to norfloxacin, erythromycin, chloramphenicol, and sulphamethoxazole-trimethoprim, respectively. However, 96.9%, 85.9%, and 50% of the isolates were resistant to ampicillin, amoxicillin, and streptomycin, respectively. Strains that developed multi-drug resistant were 68.7%. The result of this study revealed the occurrence of Campylobacter in bovine, goat, and chicken meats. Hence, there is a chance of acquiring infection via consumption of raw or undercooked meat. Thus, implementation of hygienic practices from a slaughterhouse to the retailers, proper handling and cooking of foods of meat are very important in preventing Campylobacter infection.

Download Full-text

Extended Spectrum Beta Lactamases Detection and Multiple Antibiotic Resistance Indexing of Escherichia Coli from Urine Samples of Patients from a Referral Hospital of Eastern Nepal

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v3i3.12924 ◽

2015 ◽

Vol 3 (3) ◽

pp. 423-426 ◽

Cited By ~ 1

Author(s):

A. Chakrawarti ◽

P. Dongol ◽

H. Khanal ◽

P. Subba ◽

J.J. Benerjee

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Urine Samples ◽

Multiple Antibiotic Resistance ◽

Beta Lactamases ◽

E Coli ◽

Extended Spectrum Beta Lactamases ◽

Mar Index ◽

Esbl Producers ◽

Disc Assay

Background: Escherichia coli is the most common causative agent of urinary tract infection. Antibiotic resistance among uropathogens has become a prominent public health problem. Multidrug resistance bacteria have limited the therapeutic possibilities by producing Extended Spectrum Beta Lactamases (ESBL). Objective: Since routine monitoring of ESBL producers are not conducted in clinical laboratories their true prevalence is still unknown. So the objective of this research was to assess multiple antibiotic resistance (MAR) indices and determine ESBL production among Escherichia coli isolated from urine samples. Methods: Standard microbiological techniques and antibiotic sensitivity test were performed by Kirby Bauer disc diffusion method to identify E. coli. ESBL screening was done by using Ceftriaxone, Aztreonam, Cefotaxime, Ceftazidime and Cefpodoxime whereas confirmation by combined disc assay. SPSS 16 software was used to analyze data. Results: 86.95% E. coli isolates were MDR strains. 27 isolates had multiple antibiotic resistance (MAR) index of 0.2 and 5 isolates had MAR index of 0.7. E. coli isolates showed higher degree of resistance towards Amoxicillin (100%) while 100% were sensitive towards Gentamicin followed by Nitrofurantoin (62.31%). The reliable screening agent for ESBL detection with sensitivity 100% and positive predictive value of 80% was Cefotaxime. Combined disc assay detected 12/69 (17.31%) of E. coli isolates as confirmed ESBL producers. Conclusion: The ubiquity of ESBL-producing E. coli was observed emphasizing the necessity of regular surveillance of ESBL producing clinical isolates in clinical samples to minimize multi-drug resistance strains and avert the ineffectiveness of antimicrobial agent for good health practices.\Int J Appl Sci Biotechnol, Vol 3(3): 423-426

Download Full-text

Prevalence and Loads of Fecal Pollution Indicators and the Antibiotic Resistance Phenotypes of Escherichia coli in Raw Minced Beef in Lebanon

Foods ◽

10.3390/foods9111543 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1543 ◽

Cited By ~ 1

Author(s):

Issmat I. Kassem ◽

Nivin A Nasser ◽

Joanna Salibi

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Food Safety ◽

Food Systems ◽

Meat Products ◽

Fecal Coliforms ◽

E Coli ◽

Beef Meat ◽

Minced Beef ◽

Meat Samples

Meat is an important source of high biological value proteins as well as many vitamins and minerals. In Lebanon, beef meats, including raw minced beef, are among the most consumed of the meat products. However, minced beef meat can also be an important source of foodborne illnesses. This is of a major concern, because food safety in Lebanon suffers from well-documented challenges. Consequently, the prevalence and loads of fecal coliforms and Escherichia coli were quantified to assess the microbiological acceptability of minced beef meat in Lebanon. Additionally, antibiotic resistance phenotypes of the E. coli were determined in response to concerns about the emergence of resistance in food matrices in Lebanon. A total of 50 meat samples and 120 E. coli isolates were analyzed. Results showed that 98% and 76% of meat samples harbored fecal coliforms and E. coli above the microbial acceptance level, respectively. All E. coli were resistant to at least one antibiotic, while 35% of the isolates were multidrug-resistant (MDR). The results suggest that Lebanon needs to (1) update food safety systems to track and reduce the levels of potential contamination in important foods and (2) implement programs to control the proliferation of antimicrobial resistance in food systems.

Download Full-text

Prevalence of Campylobacter, Salmonella, and Escherichia coli on the External Packaging of Raw Meat

Journal of Food Protection ◽

10.4315/0362-028x-68.3.469 ◽

2005 ◽

Vol 68 (3) ◽

pp. 469-475 ◽

Cited By ~ 31

Author(s):

F. BURGESS ◽

C. L. LITTLE ◽

G. ALLEN ◽

K. WILLIAMSON ◽

R. T. MITCHELL

Keyword(s):

Escherichia Coli ◽

External Surface ◽

Hazard Analysis ◽

Campylobacter Coli ◽

Microbiological Contamination ◽

Antimicrobial Drugs ◽

Raw Meat ◽

E Coli ◽

Meat Samples ◽

Raw Meats

During September and October 2002, 3,662 prepackaged raw meat samples were collected to evaluate the extent and nature of microbiological contamination on external surfaces of the packaging, which could potentially cross-contaminate ready-to-eat foods during and after purchase. Salmonella was detected on two (<1%) samples of external packaging (both from raw chicken), and Campylobacter was detected on 41 (1.1%) samples of external packaging. The external packaging of game fowl exhibited the highest Campylobacter contamination (3.6%), followed by raw chicken (3.0%), lamb (1.6%), turkey (0.8%), pork (0.2%), and beef (0.1%); Campylobacter jejuni and Campylobacter coli accounted for 59% (24 of 41) and 24% (10 of 41) of the contaminating Campylobacter species, respectively. C. coli isolates from the external packaging were more multiresistant to antimicrobial drugs, including quinolones such as ciprofloxacin, than was C. jejuni. Escherichia coli (an indicator of fecal contamination) was isolated from the external packaging on 4% of the raw meat samples at levels of 40 to 105 CFU per swab. The external packaging of raw meats is a vehicle for potential cross-contamination by Campylobacter, Salmonella, and E. coli in retail premises and consumers' homes. The external surface of heat-sealed packaging was less frequently contaminated with Campylobacter and E. coli compared with other types of packaging (e.g., overwrapping, bag, and tie tape) (P < 0.0001 to 0.01). In addition, external packaging of raw meats was contaminated less frequently with Campylobacter and E. coli when packaging was intact, packaging and display areas were visually clean, display temperatures were below 8°C, and hazard analysis systems were in place.

Download Full-text

The Periplasmic Protein MppA Requires an Additional Mutated Locus To Repress marA Expression in Escherichia coli

Journal of Bacteriology ◽

10.1128/jb.185.4.1465-1469.2003 ◽

2003 ◽

Vol 185 (4) ◽

pp. 1465-1469 ◽

Cited By ~ 5

Author(s):

Xiaowen Bina ◽

Vincent Perreten ◽

Stuart B. Levy

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Antibiotic Susceptibility ◽

Type Strain ◽

Wild Type Strain ◽

Binding Protein ◽

Periplasmic Protein ◽

Multiple Antibiotic Resistance ◽

Wild Type ◽

Expression In Escherichia Coli

ABSTRACT Escherichia coli strain TP985, which has an insertional mutation in the gene for the periplasmic murein tripeptide binding protein MppA, was previously reported to overproduce MarA and exhibit a multiple-antibiotic resistance (Mar) phenotype (H. Li and J. T. Park, J. Bacteriol. 181:4842-4847, 1999). We found that TP985 contained a previously unrecognized marR mutation which was responsible for the Mar phenotype. Transduction of the mppA mutation from TP985 to another wild-type strain did not affect antibiotic susceptibility. Overproduction of MppA repressed marA transcription in TP985 but not in other mppA or marR mutants. Therefore, TP985 contains an additional unknown mutation(s) that facilitates the repression of marA expression by MppA.

Download Full-text