Isolation, expression and function analysis of a bZIP transcription factor IbbZIP37 in sweetpotato (Ipomoea batatas L. [Lam])

bZIP transcription factor play an important regulatory role in the response to multiple abiotic stresses. However, our knowledge of the stress tolerance functions of bZIP family genes in sweetpotato (Ipomoea batatas [L.] Lam) remains limited. In the present study, we isolated and functionally characterized an IbbZIP37 gene encoding an abiotic stress-inducible bZIP group A transcription factor. Sequence analysis showed that the IbbZIP37 contained a typical bZIP domain and five conserved Ser/Thr kinase phosphorylation sites (RXXS/T). The IbbZIP37 protein was localized in the nucleus and possessed transcriptional activation activity. The results of electrophoretic mobility shift assays indicated that IbbZIP37 can bind to the ABRE cis-element, not do to DRE cis-element in vitro. The IbbZIP37 gene showed the highest level of constitutive expression in root, especially in fibrous root and storage root body. Gene expression was induced by ABA and several environmental stresses including drought, salt and heat shock. Our results suggest that IbbZIP37 is a positive transcription regulator of the abiotic stresses response, which can be used as an excellent candidate for improving the stress tolerance of different crop plants.

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Genome-Wide Identification and Expression Analyses of the bZIP Transcription Factor Genes in moso bamboo (Phyllostachys edulis)

International Journal of Molecular Sciences ◽

10.3390/ijms20092203 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2203 ◽

Cited By ~ 6

Author(s):

Feng Pan ◽

Min Wu ◽

Wenfang Hu ◽

Rui Liu ◽

Hanwei Yan ◽

...

Keyword(s):

Transcription Factor ◽

Stress Responses ◽

Transcriptional Activation ◽

Growth And Development ◽

Expression Profiles ◽

Forest Products ◽

Regulatory Elements ◽

Moso Bamboo ◽

Bzip Transcription Factor ◽

Phyllostachys Edulis

The basic leucine zipper (bZIP) transcription factor (TF) family is one of the largest gene families, and play crucial roles in many processes, including stress responses, hormone effects. The TF family also participates in plant growth and development. However, limited information is available for these genes in moso bamboo (Phyllostachys edulis), one of the most important non-timber forest products in the world. In the present study, 154 putative PhebZIP genes were identified in the moso bamboo genome. The phylogenetic analyses indicate that the PhebZIP gene proteins classify into 9 subfamilies and the gene structures and conserved motifs that analyses identified among all PhebZIP proteins suggested a high group-specificity. Microsynteny and evolutionary patterns analyses of the non-synonymous (Ka) and synonymous (Ks) substitution rates and their ratios indicated that paralogous pairs of PhebZIP genes in moso bamboo underwent a large-scale genome duplication event that occurred 7–15 million years ago (MYA). According to promoter sequence analysis, we further selected 18 genes which contain the higher number of cis-regulatory elements for expression analysis. The result showed that these genes are extensively involved in GA-, ABA- and MeJA-responses, with possibly different mechanisms. The tissue-specific expression profiles of PhebZIP genes in five plant tissues/organs/developmental stages suggested that these genes are involved in moso bamboo organ development, especially seed development. Subcellular localization and transactivation activity analysis showed that PhebZIP47 and PhebZIP126 were localized in the nucleus and PhebZIP47 with no transcriptional activation in yeast. Our research provides a comprehensive understanding of PhebZIP genes and may aid in the selection of appropriate candidate genes for further cloning and functional analysis in moso bamboo growth and development, and improve their resistance to stress during their life.

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Identification and characterization of the bZIP transcription factor family and its expression in response to abiotic stresses in sesame

PLoS ONE ◽

10.1371/journal.pone.0200850 ◽

2018 ◽

Vol 13 (7) ◽

pp. e0200850 ◽

Cited By ~ 19

Author(s):

Yanyan Wang ◽

Yujuan Zhang ◽

Rong Zhou ◽

Komivi Dossa ◽

Jingyin Yu ◽

...

Keyword(s):

Transcription Factor ◽

Abiotic Stresses ◽

Bzip Transcription Factor ◽

Transcription Factor Family ◽

Bzip Transcription Factor Family ◽

Identification And Characterization

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Cloning and characterization of a bZIP transcription factor gene in wheat and its expression in response to stripe rust pathogen infection and abiotic stresses

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2009.02.002 ◽

2008 ◽

Vol 73 (4-5) ◽

pp. 88-94 ◽

Cited By ~ 32

Author(s):

Yi Zhang ◽

Gang Zhang ◽

Ning Xia ◽

Xiao-Jie Wang ◽

Li-Li Huang ◽

...

Keyword(s):

Transcription Factor ◽

Stripe Rust ◽

Abiotic Stresses ◽

Bzip Transcription Factor ◽

Transcription Factor Gene ◽

Pathogen Infection ◽

Factor Gene ◽

Rust Pathogen

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Genome wide identification of NAC transcription factors and their role in abiotic stress tolerance in Chenopodium quinoa

10.1101/693093 ◽

2019 ◽

Cited By ~ 1

Author(s):

Nouf Owdah Alshareef ◽

Elodie Rey ◽

Holly Khoury ◽

Mark Tester ◽

Sandra M. Schmöckel

Keyword(s):

Transcription Factor ◽

Transcription Factors ◽

Abiotic Stress ◽

Stress Tolerance ◽

Abiotic Stresses ◽

Abiotic Stress Tolerance ◽

Chenopodium Quinoa ◽

Phosphate Starvation ◽

Water Deficiency ◽

Genome Wide

AbstractChenopodium quinoa Willd. (quinoa) is a pseudocereal with high nutritional value and relatively high tolerance to several abiotic stresses, including water deficiency and salt stress, making it a suitable plant for the study of mechanisms of abiotic stress tolerance. NAC (NAM, ATAF and CUC) transcription factors are involved in a range of plant developmental processes and in the response of plants to biotic and abiotic stresses. In the present study, we perform a genome-wide comprehensive analysis of the NAC transcription factor gene family in quinoa. In total, we identified 107 quinoa NAC transcription factor genes, distributed equally between sub-genomes A and B. They are phylogenetically clustered into two major groups and 18 subgroups. Almost 75% of the identified CqNAC genes were duplicated two to seven times and the remaining 25% of the CqNAC genes were found as a single copy. We analysed the transcriptional responses of the identified quinoa NAC TF genes in response to various abiotic stresses. The transcriptomic data revealed 28 stress responsive CqNAC genes, where their expression significantly changed in response to one or more abiotic stresses, including salt, water deficiency, heat and phosphate starvation. Among these stress responsive NACs, some were previously known to be stress responsive in other species, indicating their potentially conserved function in response to abiotic stress across plant species. Six genes were differentially expressed specifically in response to phosphate starvation but not to other stresses, and these genes may play a role in controlling plant responses to phosphate deficiency. These results provide insights into quinoa NACs that could be used in the future for genetic engineering or molecular breeding.

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