Constitutive expression of Arabidopsis bZIP transcription factor AREB1 activates cross-signaling responses in soybean under drought and flooding stresses

2021 ◽  
Vol 257 ◽  
pp. 153338
Author(s):  
Martina Bianca Fuhrmann-Aoyagi ◽  
Claudete de Fátima Ruas ◽  
Elton Gargioni Grisoste Barbosa ◽  
Patricia Braga ◽  
Larissa Alessandra Cardoso Moraes ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Constitutive Expression ◽  
Bzip Transcription Factor

scholarly journals Isolation, expression and function analysis of a bZIP transcription factor IbbZIP37 in sweetpotato (Ipomoea batatas L. [Lam])

2019 ◽  
pp. 134
Author(s):  
Wenbin Wang, Xiangpo Qiu ◽  
Yanxin Yang ◽  
Ho-Soo Kim ◽  
Xiaoyun Jia, Huan Yu ◽  
Sang-Soo Kwak
Keyword(s):  
Transcription Factor ◽  
Stress Tolerance ◽  
Transcriptional Activation ◽  
Abiotic Stresses ◽  
Ipomoea Batatas ◽  
Function Analysis ◽  
Constitutive Expression ◽  
Bzip Transcription Factor ◽  
Fibrous Root ◽  
Cis Element

bZIP transcription factor play an important regulatory role in the response to multiple abiotic stresses. However, our knowledge of the stress tolerance functions of bZIP family genes in sweetpotato (Ipomoea batatas [L.] Lam) remains limited. In the present study, we isolated and functionally characterized an IbbZIP37 gene encoding an abiotic stress-inducible bZIP group A transcription factor. Sequence analysis showed that the IbbZIP37 contained a typical bZIP domain and five conserved Ser/Thr kinase phosphorylation sites (RXXS/T). The IbbZIP37 protein was localized in the nucleus and possessed transcriptional activation activity. The results of electrophoretic mobility shift assays indicated that IbbZIP37 can bind to the ABRE cis-element, not do to DRE cis-element in vitro. The IbbZIP37 gene showed the highest level of constitutive expression in root, especially in fibrous root and storage root body. Gene expression was induced by ABA and several environmental stresses including drought, salt and heat shock. Our results suggest that IbbZIP37 is a positive transcription regulator of the abiotic stresses response, which can be used as an excellent candidate for improving the stress tolerance of different crop plants.  

scholarly journals A combinatorial action of GmMYB176 and GmbZIP5 controls isoflavonoid biosynthesis in soybean (Glycine max)

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Arun Kumaran Anguraj Vadivel ◽  
Tim McDowell ◽  
Justin B. Renaud ◽  
Sangeeta Dhaubhadel
Keyword(s):  
Transcription Factor ◽  
Hairy Roots ◽  
Defense Mechanisms ◽  
Biosynthetic Pathway ◽  
Bzip Transcription Factor ◽  
Myb Transcription Factor ◽  
In Planta ◽  
Rnai Silencing ◽  
Protein Protein Interaction ◽  
Soybean Roots

AbstractGmMYB176 is an R1 MYB transcription factor that regulates multiple genes in the isoflavonoid biosynthetic pathway, thereby affecting their levels in soybean roots. While GmMYB176 is important for isoflavonoid synthesis, it is not sufficient for the function and requires additional cofactor(s). The aim of this study was to identify the GmMYB176 interactome for the regulation of isoflavonoid biosynthesis in soybean. Here, we demonstrate that a bZIP transcription factor GmbZIP5 co-immunoprecipitates with GmMYB176 and shows protein–protein interaction in planta. RNAi silencing of GmbZIP5 reduced the isoflavonoid level in soybean hairy roots. Furthermore, co-overexpression of GmMYB176 and GmbZIP5 enhanced the level of multiple isoflavonoid phytoallexins including glyceollin, isowighteone and a unique O-methylhydroxy isoflavone in soybean hairy roots. These findings could be utilized to develop biotechnological strategies to manipulate the metabolite levels either to enhance plant defense mechanisms or for human health benefits in soybean or other economically important crops.

Interaction between optineurin and the bZIP transcription factor NRL

2013 ◽  
Vol 38 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Chunxia Wang ◽  
Katsuhiro Hosono ◽  
Masafumi Ohtsubo ◽  
Kentaro Ohishi ◽  
Jie Gao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Bzip Transcription Factor

ADH2 expression is repressed by REG1 independently of mutations that alter the phosphorylation of the yeast transcription factor ADR1

1993 ◽  
Vol 13 (7) ◽  
pp. 4391-4399
Author(s):  
K M Dombek ◽  
S Camier ◽  
E T Young
Keyword(s):  
Transcription Factor ◽  
Glucose Repression ◽  
Phosphorylation Site ◽  
Constitutive Expression ◽  
Partial Activation ◽  
Dependent Protein ◽  
Multiple Levels ◽  
Kinase Phosphorylation ◽  
Protein Kinase Phosphorylation Site ◽  
Dependent Pathway

In Saccharomyces cerevisiae, expression of the ADH2 gene is undetectable during growth on glucose. The transcription factor ADR1 is required to fully activate expression when glucose becomes depleted. Partial activation during growth on glucose occurred in cells carrying a constitutive allele of ADR1 in which the phosphorylatable serine of a cyclic AMP (cAMP)-dependent protein kinase phosphorylation site had been changed to alanine. When glucose was removed from the growth medium, a substantial increase in the level of this constitutive expression was observed for both the ADH2 gene and a reporter construct containing the ADR1 binding site. This suggests that glucose can block ADR1-mediated activation independently of cAMP-dependent phosphorylation at serine 230. REG1/HEX2/SRN1 was identified as a potential serine 230-independent repressor of ADH2 expression. Yeast strains carrying a deletion of the REG1 gene, reg1-1966, showed a large increase in ADR1-dependent expression of ADH2 during growth on glucose. A smaller increase in ADR1-independent expression was also observed. Additionally, an increase in the level of ADR1 expression and posttranslational modification of the ADR1 protein were observed. When the reg1-1966 allele was combined with various ADR1 constitutive alleles, the level of ADH2 expression was synergistically elevated. This indicates that REG1 can act independently of phosphorylation at serine 230. Our results suggest that glucose repression in the presence of ADR1 constitutive alleles occurs primarily through a REG1-dependent pathway which appears to affect ADH2 transcription at multiple levels.

Altered expression of the bZIP transcription factor DRINK ME affects growth and reproductive development inArabidopsis thaliana

2016 ◽  
Vol 88 (3) ◽  
pp. 437-451 ◽  
Author(s):  
Paulina Lozano-Sotomayor ◽  
Ricardo A. Chávez Montes ◽  
Marina Silvestre-Vañó ◽  
Humberto Herrera-Ubaldo ◽  
Raffaella Greco ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Reproductive Development ◽  
Bzip Transcription Factor ◽  
Altered Expression

scholarly journals Thyrotroph embryonic factor (TEF) is differentially expressed in high-grade serous ovarian cancers.

2020 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Ovarian Cancer ◽  
Transcription Factor ◽  
Differential Expression ◽  
Expression Profiles ◽  
Gynecologic Cancer ◽  
Bzip Transcription Factor ◽  
High Grade ◽  
Significant Differential Expression ◽  
Primary Tumors ◽  
Embryonic Factor

Ovarian cancer is the most lethal gynecologic cancer (1). We sought to identify genes associated with high-grade serous ovarian cancer (HGSC) by comparing global gene expression profiles of normal ovary with that of primary tumors from women diagnosed with HGSC using published microarray data (2, 3). We previously reported differential expression of the PAR-bZIP transcription factor HLF in HGSC (4). Here, we report significant differential expression of a second PAR-bZIP transcription factor, thyrotroph embryonic factor (TEF) (5) in high-grade serous ovarian tumors.

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