Population and Nesting Behaviour of Weaver Ants, Oecophylla smaragdina from Meghalaya, India

Weaver ants are known for their unique nest-building skills using leaves and larval-silk as a binding agent. The weaver ants, Oecophylla smaragdina are present in large numbers in the Ri-Bhoi district, Meghalaya. Ri-Bhoi district is a hot and humid place with 22 -30°C. This is the first study from this region to examine the nesting behavior along with the population of these ants from here. It was noted that they build nests in different types of trees but it is more abundant in needlewood trees (Schima wallichi) locally known as ‘diengngan.’ From the central trunk of the S. wallichi tree, the nearest distance of the nest is about 0.7 m and the farthest up to 3.4 m. The nests of O. smaragdina are somewhat round-oval and use leaves of different sizes ranging from 8-32 cm2. The nests are made at a height ranging from 4-25 m and their average nest size is about 9,483 cm3. They utilize about twenty leaves with a specific number of chambers to keep their broods, the queen, and food. The number of worker ants, pupae, and larvae are variable in different nests because of nest size, location/height of the nests, and the trees. When the nest population increases, they locate a new spot and build a satellite nest where they get the right amount of sunlight and shelter from predators and adverse environmental factors. These weaver ants are also used as medicine, food and fish bait by the indigenous people in Meghalaya.

Seabird Tissue Archival and Monitoring Project (STAMP) Data from 1999-2010

The multi-entity, long-term Seabird Tissue Archival and Monitoring Project (STAMP) has collected eggs from various avian species throughout the North Pacifc Ocean for over 20 years to create a geospatial and temporal record of environmental conditions. Over 2,500 samples are currently archived at the NIST Biorepository at Hollings Marine Laboratory in Charleston, South Carolina. Longitudinal monitoring efforts of this nature provide invaluable data for assessment of both wildlife and human exposures as these species often consume prey (e.g., fish) similar to, and from sources (e.g., oceanic) comparable to, human populations nearby. In some areas, seabird eggs also comprise a signifcant part of subsistence diets providing nutrition for indigenous peoples. Chemometric profles and related health implications are known to differ across species. Eggs, however, can be diffcult to assign to a species unless the bird is observed on the nest from which the sample was collected due to similar appearance within a genus and sympatric nesting behavior. This represents a large point of uncertainty for both wildlife managers and exposure researchers alike.

Distribution, habitat use, and nesting behavior of the King Cobra (Ophiophagus hannah) in the Trashigang Forest Division, Eastern Bhutan

The King Cobra, Ophiophagus hannah (Cantor 1836), is the longest venomous snake in the world; it is globally threatened and has been categorized as Vulnerable (VU) on the IUCN Red List. Herein we describe distribution, habitats, and nesting behavior of the King Cobra in Trashigang Forest Division, Bhutan. We recorded a total of 18 King Cobras during the one-year survey period. The elevational distribution was 650–2,057 m but most sightings were at lower elevations. King Cobras did not appear to prefer or avoid any particular habitat type but most records were from areas where human disturbances were at a minimum. We monitored a nest containing 37 eggs that was actively guarded by a female. This study was the first of its kind in Trashigang Forest Division and we hope that the information obtained will help formulate appropriate conservation strategies for this globally threatened species.

Nesting Biology of the Solitary Ground-Nesting Bee Diadasina riparia (Apidae: Emphorini)

We described the nesting behavior and ecology of Diadasina riparia, from an urban dry forest fragment. The nests of D. riparia were shallow with circular entrance, closed by a mud plug, from which a straight vertical tunnel ending in one or more brood cells. Most females need one day for construction and provisioning the nest. The natural enemies were flies of Anthrax genus, the cleptoparasitic bees Leiopodus trochantericus, and Mutillidae wasp. The feature about the life history, nest architecture, materials used, pollen provisions behavior and associated organisms of this species allow the comparison with other emphorines species.

A novel mouse model of postpartum depression using emotional stress as evaluated by nesting behavior

Postpartum depression is an important mental health issue not only for the mother but also for the child’s development, other family members, and the society. An appropriate animal model is desired to elucidate the pathogenesis of postpartum depression. However, methods for stress loading during pregnancy have not been established. Behavioral experiments to investigate postpartum depression-like behaviors should be conducted without stress because behavioral tests affect rearing behaviors such as lactation. Therefore, we developed a new mouse model of postpartum depression using a psychological stress method. Mating partners were made to witness their partners experiencing social defeat stress and then listen to their cries. Emotional stress loading during pregnancy significantly increased postpartum depression-like behaviors. Postpartum depression also affected nurturing behaviors and caused disturbances in pup care. Furthermore, nesting behavior was impaired in the stressed group, suggesting that the observation of nesting behavior may be useful for assessing social dysfunction in postpartum depression. These results demonstrate the utility of this new mouse model of postpartum depression.

PSXIII-21 Water consumption evolution during later gestation is associated to the farrowing day

The aim of this study was to verify the utility of water consumption as an indicator to early predict the farrowing day. A total of 28 flowmeters (iPERL®) were installed in two farrowing rooms (12 + 16 places) to record every hour the individual water consumption from the entry of the sows (Landrace × Large White) in the farrowing unit until parturition, along 5 consecutive batches (120 sows). Up to 200,377 records were managed using Python code. Data were analyzed by using the MIXED procedure of SAS. Parity (gilts, G; sows, S), time slot (morning, M: 6-14h; afternoon, A: 14-22h; night, N: 22-6h) and pre-farrowing days (-9 to -1) and farrowing (0) were included as fixed factors and the farrowing crate as a random effect. Water consumption was lower in gilts (G: 6.9 ± 1.12 L/d vs S: 8.5 ± 1.09 L/d; P < .0001). Up to 57.5% of water consumption occurred in the morning (M: 13.1 ± 1.11 L), 31.0% (A: 7.1 ± 1, 11 L) during the afternoon/evening and 11.8% (N: 2.7 ± 1.11 L) at night. From day -9 (4.9 ± 1.29 L) to -3 (9.2 ± 1.17 L), water intake lineally increased (around 0.8 L/d), highlighting the increase in 2 L at day -2 (11.2 ± 1.16 L; P < .0001). This peak could be related to a greater manipulation of the drinker due to nesting behavior. Finally, on day -1 a drastic and significant decrease in intake was observed (7.3 ± 1.16 L; P < .0001), just before the decrease in feed intake on day 0. The results suggest that water intake can be a valid indicator to early predict the day of farrowing, thus allowing foremen to better plan the management routines in the farrowing unit, especially in large sow farms.

EK100 and Antrodin C Improve Brain Amyloid Pathology in APP/PS1 Transgenic Mice by Promoting Microglial and Perivascular Clearance Pathways

Alzheimer’s disease (AD) is characterized by the deposition of β-amyloid peptide (Aβ). There are currently no drugs that can successfully treat this disease. This study first explored the anti-inflammatory activity of seven components isolated from Antrodia cinnamonmea in BV2 cells and selected EK100 and antrodin C for in vivo research. APPswe/PS1dE9 mice were treated with EK100 and antrodin C for one month to evaluate the effect of these reagents on AD-like pathology by nesting behavior, immunohistochemistry, and immunoblotting. Ergosterol and ibuprofen were used as control. EK100 and antrodin C improved the nesting behavior of mice, reduced the number and burden of amyloid plaques, reduced the activation of glial cells, and promoted the perivascular deposition of Aβ in the brain of mice. EK100 and antrodin C are significantly different in activating astrocytes, regulating microglia morphology, and promoting plaque-associated microglia to express oxidative enzymes. In contrast, the effects of ibuprofen and ergosterol are relatively small. In addition, EK100 significantly improved hippocampal neurogenesis in APPswe/PS1dE9 mice. Our data indicate that EK100 and antrodin C reduce the pathology of AD by reducing amyloid deposits and promoting nesting behavior in APPswe/PS1dE9 mice through microglia and perivascular clearance, indicating that EK100 and antrodin C have the potential to be used in AD treatment.