Improvement of Hyperlipidemia by Aerobic Exercise in Mice through a Regulatory Effect of miR-21a-5p on its Target Genes

Background and aims Hyperlipidemia is a risk factor for cardiovascular disease, and miR-21a-5p plays an important role in the occurrence and progression of hyperlipidemia, this study aimed to investigate the mechanism of aerobic exercise improved hyperlipidemia through enhancement miR-21a-5p expression.Method Spectrophotometer detected blood lipid level; HE stained liver tissue to check the grade of liver steatosis; QPCR and Western blot detected mRNA expression and protein levels. Results There had a notable improvement in the weight gain, blood lipid level and liver steatosis in hyperlipidemia mice after 8 weeks aerobic exercise intervention. In addition, aerobic exercise significantly up-regulated the expression of miR-21a-5p, and provoked favorable changes in the expression of target genes. Knockdown of miR-21a-5p resulted in dysregulation of lipid metabolism and increased expression of target genes FABP7, HMGCR, ACAT1 and OLR1. While aerobic exercise could alleviate miR-21a-5p knock-down induced lipid metabolism disorder. Conclusion Aerobic exercise improved hyperlipidemia through miR-21a-5p-induced inhibition of target genes FABP7, HMGCR, ACAT1 and OLR1.

Ozone autohemotherapy elevates PPAR-γ expression to lower blood lipid in treatment for psoriasis

Background: Psoriasis is widely accepted as a metabolic syndrome with significantly abnormal lipid metabolism and high blood lipids keep patients in a persistent low level of inflammatory condition. Hyperlipidemia and associated inflammatory reaction are believed to be the major risk factors contributing to the onset and recurrence of psoriasis. Peroxisome proliferator activated receptor-gamma (PPAR-γ) can effectively control the blood lipid level and inhibit inflammatory reaction. Methods: Ozone autohemotherapy (OAHT) was applied to treat psoriatic patients. The psoriasis area and severity index (PASI) score and blood lipid level were used to evaluate the efficacy. PPAR-γ expression level and correlation analysis were used to determine the OAHT target involving psoriasis. Results: We found that OAHT significantly decreased patients’ PASI scores and lipid blood level. Furthermore, we found that PPAR-γ expression in CD4+ T cells from patients with psoriasis was significantly lower than healthy controls, furtherly, we detected PPAR-γ expression upregulated after treatment compared with before treatment. There, we performed the correlation analysis on PPAR-γ level and patients’ PASI scores or lipid blood level. These results suggested OAHT might obviously improve patients’ PASI scores and decrease lipid blood level by regulating PPAR-γ expression. Conclusions: We found that OAHT can attenuate the condition of psoriatic patients and lower blood lipid by inducing PPAR-γ expression, suggesting that OAHT is effective in treating psoriasis and is worthy of further evaluations for its clinical applications.

A self-assembled DNA tetrahedron as a carrier for in vivo liver-specific delivery of siRNA

Liver-targeted DNA tetrahedron was employed as the carrier for liver-specific delivery of siRNA. Liver delivery of siRNA targeting ApoB1 mRNA, which is involved with hypercholesterolemia, lowered the blood lipid level.