scholarly journals Impact of the Purification Process on the Spray-Drying Performances of the Three Families of Lipopeptide Biosurfactant Produced by Bacillus subtilis

2021 ◽  
Vol 9 ◽  
Author(s):  
Antoine Vassaux ◽  
Marie Rannou ◽  
Soline Peers ◽  
Théo Daboudet ◽  
Philippe Jacques ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Spray Drying ◽  
Freeze Drying ◽  
Industrial Applications ◽  
Purification Process ◽  
Purification Step ◽  
Wide Range ◽  
Lipopeptide Biosurfactant ◽  
The Impact ◽  
Spray Dried

Lipopeptides produced by Bacillus subtilis display many activities (surfactant, antimicrobial, and antitumoral), which make them interesting compounds with a wide range of applications. During the past years, several processes have been developed to enable their production and purification with suitable yield and purity. The already implemented processes mainly end with a critical drying step, which is currently achieved by freeze-drying. In this study, the possibility to replace this freeze-drying step with a spray-drying one, more suited to industrial applications, was analyzed. After evaluating their thermal resistance, we have developed a spray-drying methodology applicable for the three lipopeptides families produced by B. subtilis, i.e., surfactin, mycosubtilin (iturin family), and plipastatin (fengycin family). For each lipopeptide, the spray-drying procedure was applied at three steps of the purification process by ultrafiltration (supernatant, diafiltered solution, and pre-purified fraction). The analysis of the activities of each spray-dried lipopeptide showed that this drying method is not decreasing its antimicrobial and biosurfactant properties. The methodology developed in this study enabled for the first time the spray-drying of surfactin, without adjuvants’ addition and regardless of the purification step considered. In the case of fengycin and mycosubtilin, only diafiltered solution and purified fraction could be successfully spray-dried without the addition of adjuvant. Maltodextrin addition was also investigated as the solution for the direct drying of supernatant. As expected, the performances of the spray-drying step and the purity of the powder obtained are highly related to the purification step at which the product was dried. Interestingly, the impact of mycosubtilin concentration on spray-drying yield was also evidenced.

scholarly journals Freeze versus Spray Drying for Dry Wild Thyme (Thymus serpyllum L.) Extract Formulations: The Impact of Gelatin as a Coating Material

2021 ◽  
Author(s):  
Aleksandra A. Jovanović ◽  
Steva M. Lević ◽  
Vladimir B. Pavlovic ◽  
Smilja B. Markovic ◽  
Rada V. Pjanovic ◽  
...  
Keyword(s):  
Spray Drying ◽  
Dry Weight ◽  
Drying Process ◽  
Scanning Calorimetry ◽  
Coating Agent ◽  
Microwave Assisted ◽  
Freeze Dried ◽  
Wild Thyme ◽  
The Impact ◽  
Spray Dried

Freeze drying was compared with spray drying regarding feasibility to process wild thyme drug in order to obtain dry formulations at laboratory scale starting from liquid extracts produced by different extraction methods: maceration, heat-, ultrasound-, and microwave-assisted extractions. Higher powder yield (based on the dry weight prior to extraction) was achieved by freeze than spray drying and lower loss of total polyphenol content (TPC) and total flavonoid content (TFC) due to the drying process. Gelatin as a coating agent (5% w/w) provided better TPC recovery by 70% in case of lyophilization and higher powder yield in case of spray drying by diminishing material deposition on the wall of the drying chamber. The resulting gelatin-free and gelatin-containing powders carried polyphenols in amount ~190 and 53-75 mg gallic acid equivalents GAE/g of powder, respectively. Microwave-assisted extract formulation distinguished from others by higher content of polyphenols, proteins and sugars, higher bulk density and lower solubility. The type of the drying process affected mainly position of the gelatin-derived -OH and amide bands in FTIR spectra. Spray dried formulations compared to freeze dried expressed higher thermal stability as confirmed by differential scanning calorimetry analysis and higher diffusion coefficient; the last feature can be associated with the lower specific surface area of irregularly shaped freeze-dried particles (151-223 µm) compared to small microspheres (~8 µm) in spray-dried powder.

scholarly journals Effect of Thermal Processing on Antioxidant Activity and Cytotoxicity of Waste Potato Juice

2019 ◽  
Vol 14 (1) ◽  
pp. 150-157 ◽  
Author(s):  
Przemysław Łukasz Kowalczewski ◽  
Anna Olejnik ◽  
Wojciech Białas ◽  
Piotr Kubiak ◽  
Aleksander Siger ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Low Temperature ◽  
Cytotoxic Activity ◽  
Spray Drying ◽  
Cell Lines ◽  
Freeze Drying ◽  
Cytotoxic Activities ◽  
Normal Cells ◽  
Stabilization Methods ◽  
The Impact

AbstractPotato juice (PJ), commonly considered a burdensome waste, is rich in various compounds with bioactive properties. It has long been considered a remedy for gastric problems in traditional folk medicine. If valorization of PJ through implementation in the production of functional foods is to be considered, stabilization methods must be developed to allow long-term storage of this seasonal product. It is important that such methods are chosen with regard to their effect on the bioactive value of the obtained product. In this study, the impact of four stabilization methods on the antioxidant and cytotoxic activities of PJ was investigated. Elevated temperatures were used in thermal deproteinization used to obtain DPJW (deproteinated potato juice water) and spray-drying of FPJ (fresh potato juice) that resulted in SDPJ. Freeze drying and cryoconcentration were the low temperature processing methods that yielded PJL (potato juice lyophilisate) and CPJ (cryocorncentrated potato juice), respectively. All processed materials were characterized chemically and compared with raw materials in terms of phenolic compounds content, antioxidant activity as well as cytotoxicity to human tumor cells isolated from the gastric mucosa (Hs476T cell line), colon (Caco-2 and HT-29 cell lines), and normal cells isolated from the small intestine and colon epithelium (IEC-6 and NCM460 cell lines). It was stated that high-temperature processes – thermal deproteinization and spray-drying – yielded products with increased antioxidant potential (TEAC) that also showed increased cytotoxic activity towards intestinal cancer cells. At the same time the cytotoxicity towards normal cells remained on par with that of fresh PJ (IEC-6 cells) or decreased (NCM460 cells). Thermal deproteinization significantly decreased the content of glycoalcaloids in the juice, while spray drying did not have such an effect. The two low-temperature processes investigated – cryoconcentration and freeze drying – did not affect the PJ cytotoxic activity towards any of the cell lines used in the tests, whereas they did affect the antioxidant properties and glycoalcaloids content of PJ.

Experimental study of passive vibration suppression using absorber with spherical ball impact damper

2016 ◽  
Vol 231 (17) ◽  
pp. 3193-3201 ◽  
Author(s):  
Riadh Chaari ◽  
Fathi Djemal ◽  
Fakher Chaari ◽  
Mohamed Slim Abbes ◽  
Mohamed Haddar
Keyword(s):  
Vibration Suppression ◽  
Industrial Applications ◽  
Design Parameters ◽  
Particle Impact ◽  
Ball Impact ◽  
Impact Damper ◽  
Ball Size ◽  
Wide Range ◽  
The Impact ◽  
Impact Damping

Impact dampers are efficient in many industrial applications with a wide range of frequencies. An experimental analysis of the impact damping of spherical balls is investigated to simplify the particle impact damping design and improve the vibration suppression. The objective of the study is to analyze some of the design parameters of impact damper using spherical balls. The experimental investigation consists to test the effect of the ball size for each mass level, the number of balls for each size level and different exciting force levels on vibrations of the main structure. The parametric study provided useful information to understand and optimize Particle Impact Damping design.

Laser Forming of Metal Foam Sandwich Panels: Effect of Panel Manufacturing Method

2019 ◽  
Vol 141 (5) ◽  
Author(s):  
Tizian Bucher ◽  
Min Zhang ◽  
Chang Jun Chen ◽  
Ravi Verma ◽  
Wayne Li ◽  
...  
Keyword(s):  
Metal Foam ◽  
Numerical Models ◽  
Sandwich Panels ◽  
Weight Ratio ◽  
Industrial Applications ◽  
Laser Forming ◽  
Process Conditions ◽  
Foam Core ◽  
Wide Range ◽  
The Impact

Sandwich panels with metal foam cores have a tremendous potential in various industrial applications due to their outstanding strength-to-weight ratio, stiffness, and shock absorption capacity. A recent study paved the road toward a more economical implementation of sandwich panels, by showing that the material can be successfully bent up to large angles using laser forming. The study also developed a fundamental understanding of the underlying bending mechanisms and established accurate numerical models. In this study, these efforts were carried further, and the impact of the foam core structure, the facesheet and foam core compositions, and the adhesion method on the bending efficiency and the bending limit was investigated. These factors were studied individually and collectively by comparing two fundamentally different sandwich panel types. Thermally induced stresses at the facesheet/core interface were thoroughly considered. Numerical modeling was carried out under different levels of geometric accuracy to complement bending experiments under a wide range of process conditions. Interactions between panel properties and process conditions were demonstrated and discussed.

Preparation and characterization of dried cellulose nanofibrils

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Jianping Ni ◽  
Chen Gong ◽  
Zhenghua Su ◽  
Chao Tian
Keyword(s):  
Spray Drying ◽  
Freeze Drying ◽  
Cellulose Nanofibrils ◽  
Time Dependent ◽  
X Ray ◽  
Original State ◽  
Final Mass ◽  
Freeze Dried ◽  
Spray Dried

Abstract One of the main manufacturing challenges is to obtain dried cellulose nanofibrils (CNFs) so that they can be cost effectively transported to customers. This work presents a study on using two methods of drying: freeze drying and spray drying; these dried CNFs were then characterized. The dried CNFs from either freeze drying or spray drying could not recover their original state after simple re-dispersion in water. Compared to spray dried CNFs, the microstructure of the freeze dried CNFs remained in a better shape. This was because the packing of nanofibrils as a result of freeze drying was not as tight as that from spray drying. It was demonstrated by the lower final mass residue and crystallinity of the freeze-dried CNFs, which led to better re-dispersion in water. X-ray diffractometry proved the occurrence of aggregation/hornification of the dried CNFs with increased crystallinity. Time-dependent sedimentation confirmed that the dried CNFs were incapable of forming stable water-re-dispersible suspensions.

scholarly journals Effects of Vacuum Freeze-Drying and Vacuum Spray-Drying on Biochemical Properties and Functionalities of Myofibrillar Proteins from Silver Carp

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Jianzhen Niu ◽  
Binfan Zhao ◽  
Xiujin Guo ◽  
Tao Yin
Keyword(s):  
Spray Drying ◽  
Freeze Drying ◽  
Silver Carp ◽  
Biochemical Properties ◽  
Molecular Structures ◽  
Drying Methods ◽  
Myofibrillar Proteins ◽  
Fish Protein ◽  
Freeze Dried ◽  
Spray Dried

Fish protein powders were produced from silver carp myofibrillar proteins using vacuum freeze-drying and vacuum spray-drying. Biochemical properties and functionalities of freeze-dried and spray-dried powders were determined. The myofibrillar proteins were partially denatured under both the drying methods which were evidenced by the increase of free sulfhydryl content, surface hydrophobicity, and intrinsic fluorescence while the decrease of the Ca2+-ATPase activity and percentage of the α-helical structure. With respect to vacuum freeze-drying, the proteins were denatured to a higher degree by vacuum spray-drying. The spray-dried fish protein powder showed a higher water retention capacity and emulsifying stability index, but the same solubility and emulsifying activity index. The micrographs indicate that vacuum freeze-dried powder formed a spongy structure, while the powder under vacuum spray-drying mostly appeared spherical in shape with hollow inside. Thus, the two drying methods can be used to manufacture fish protein powders with varied molecular structures and functionalities.

scholarly journals Optimization of Spray-Drying Process of Jerusalem artichoke Extract for Inulin Production

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1674
Author(s):  
Zhenzhou Zhu ◽  
Mailing Wu ◽  
Jie Cai ◽  
Shuyi Li ◽  
Krystian Marszałek ◽  
...  
Keyword(s):  
Particle Size ◽  
Water Content ◽  
Spray Drying ◽  
Freeze Drying ◽  
Heating Temperature ◽  
Great Influence ◽  
Jerusalem Artichoke ◽  
Drying Methods ◽  
Freeze Dried ◽  
Spray Dried

Jerusalem artichoke is an important natural matrix for inulin production. In this experiment, response surface methodology (RSM) was employed to optimize the spray-drying parameters in order to determine the maximal inulin yield. For this study, three independent variables (heating temperature (Tª, 110–120 °C), creep speed (V, 18–22 rpm) and pressure (P, 0.02–0.04 MPa)) were used in the experimental design. Using the Box–Behnken design, the optimal parameters obtained were: drying temperature 114.6 °C, creep speed 20.02 rpm, and pressure: 0.03 MPa. The inulin yield, water content and particle size of inulin obtained by spray-drying and freeze-drying were compared. In this regard, the spray-dried inulin consisted of a white powder having a fine particle size, and the freeze-dried inulin had a pale-yellow fluffy floc. On the other hand, the drying methods had a great influence on the appearance and internal structure of inulin powder, since the spray-dried inulin had a complete and uniform shape and size, whereas the freeze-dried inulin had a flocculated sheet structure. The analysis showed that the spray-drying led to a higher inulin yield, lower water content and better surface structure than freeze-drying.

Characterization of Lipopeptides Biosurfactants Produced by a Newly Isolated Strain Bacillus Subtilis ZNI5: Potential Environmental Application

2021 ◽  
Author(s):  
Mnif Inès ◽  
Hayfa Rajhi ◽  
Amir Bouallegue ◽  
Nour Trabelsi ◽  
Dhouha Ghribi
Keyword(s):  
Surface Tension ◽  
Bacillus Subtilis ◽  
High Performance ◽  
Surface Active Agent ◽  
Active Agent ◽  
Industrial Applications ◽  
Exchange Chromatography ◽  
Environmental Application ◽  
Molecular Weights ◽  
Lipopeptide Biosurfactant

Abstract Strain ZNI5, isolated from a hydrocarbon contaminated soil and identified as Bacillus subtilis after 16s rDNA sequence, grew and produced lipopeptides biosurfactants when cultured on glucose based media. After purification by anionic exchange chromatography and identification Reverse Phase High Performance Liquid Chromatography-Mass Spectrometry, the biosurfactant produced by ZNI5 were determined to be cyclic lipopeptides homologues. Four families of lipopeptides were identified by HPLC-MS analysis. They belongs to surfactin isoforms with molecular weights of 979, 993, 1007, 1021 and 1035 Da; iturin isoforms with molecular weights of 1028, 1042 and 1056 Da; Licheniformin with molecular weight of 1410 and newly identified isoforms named Inesfactin with molecular weights of 973 and 987 Da. Functional properties of the ZNI5 biosurfactant were studied. It was characterized as a powerful surface-active agent that decreases the surface tension of water from 72 mN/m to about 32 mN/m with a CMC value of 350 mg/L more efficient than chemical surfactants (Triton X100; CTAB and SDS). It has the capacity to disperse oil to about 80 mm at a concentration of 800 mg/L showing close efficiencies to the listed chemical surfactants. In addition, by studying the surface tension decrease capacity and the oil displacement activity, ZNI5 lipopeptide biosurfactant showed great thermal, pH and salts activity and stability enabling its use in the bioremediation fields and for diverse industrial applications.

A Comparison of Spray-Drying and Freeze-Drying for the Production of Stable Silybin Nanosuspensions

2020 ◽  
Vol 20 (6) ◽  
pp. 3598-3603 ◽  
Author(s):  
Yingying Ma ◽  
Jin Gao ◽  
Wankui Jia ◽  
Yangyang Liu ◽  
Lanying Zhang ◽  
...  
Keyword(s):  
Spray Drying ◽  
Freeze Drying ◽  
Drying Methods ◽  
Dry Powders ◽  
Term Stability ◽  
Long Term Stability ◽  
Freeze Dried ◽  
Spray Dried

Spray-drying and freeze-drying are effective approaches to improve the long-term stability of nanosuspensions. This research explored the effect of spray-drying and freeze-drying techniques on PVP K30-stabilized silybin nanosuspensions. The morphology was observed by scanning electron microscopy (SEM): The spray-dried sample was spherical, and the freeze-dried samples were rodlike with smooth surfaces. The redispersibility was studied via dynamic light scattering (DLS): The size, PDI, and zeta of the spray-dried sample were 133.27 nm, 0.214, and 24.37 mV, respectively; the size, PDI, and zeta of the freeze-dried sample were 298.70 nm, 0.114, and 20.98 mV, respectively. The in vitro dissolution was studied, and the two dry powders showed a significant increase compared to silybin. The two dried powders had better long-term stability than the liquid starting material. Overall, spray-drying and freeze-drying are appropriate drying methods for the preparation of silybin nanosuspensions with better stability and dissolution velocity.

scholarly journals Surface ModificationTo Reduce Deposition Flux of Spray Dried Noni (Morinda Citrofolia L.) Juice

2019 ◽  
Vol 16 (04) ◽  
pp. 743-750
Author(s):  
Noraziani Zainal Abidin ◽  
Saiful Irwan Zubairi ◽  
Haslaniza Hashim
Keyword(s):  
Contact Angle ◽  
Surface Modification ◽  
Spray Drying ◽  
Negative Relationship ◽  
Hydrophobic Surface ◽  
Surface Hydrophobicity ◽  
Inlet Temperature ◽  
Deposition Flux ◽  
The Impact ◽  
Spray Dried

ABSTRACT: Spray drying is widely spread technique for drying of liquid products especially fruit juices such as noni juice. However, during spray drying of noni juice stickiness problem commonly occurs due to low molecular weight sugars (sucrose, glucose and fructose). The aim of the present research was to determine the impact of Teflon surface modification on the yield of spray dried Noni juice and deposition flux. Different exposure time of feed spray was undertaken prior to adhesion flux weight determination. Three different sets of inlet temperature (100 oC, 150 OC and 190 oC) and exposure times (of 15 min, 30 min, 60 min, 75 min, 90 min and 115 min) were tested. Samples were evaluated for contact angle, weight of adhesion flux, hygroscopicity and moisture content. There was a sudden increased in deposition flux (p<0.05) as the temperature increased. At 150 and 190°C, the weight of deposition flux was not significant (p>0.05) for both plate materials. The borosilicate plates (BP) registered up to 80% deposit removal whilst Teflon plate (TP) recorded only 20%. Physical observation of the Teflon plates showed that some areas of the plate (100 °C) were fully clear from any particles. Nevertheless, for the borosilicate glass plates, the primary adhered particles tend to retain and smeared along the plates. In addition, there was a substantially negative relationship between the contact angle and adhesion weight, (r = -0,924, p<0.01). The negative connection suggests that by increasing the contact angle, the adhesion weight will decrease and vice versa. The contact angle of the Teflon (PTEFE) surface was approximately 117.2 ± 1.4°, which provided an excellent hydrophobic surface. In this research, the powder with the lowest amount of hygroscopicity was gained at 190 oC using the Teflon plate. For that reason, surface modification was indeed amplified the surface hydrophobicity thus decreasing the deposition flux.

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