INFLUENCE OF TEMPERATURE AND PLANTING DENSITY ON PERIPHERAL BLOOD PARAMETERS OF THE AFRICAN SHARPTOOTH CATFISH IN THE CONDITIONS OF RAS

The article presents the results of research on the influence of biotic and abiotic factors, in particular, planting density and temperature on the peripheral blood composition of the African sharptooth catfish. It was found that when the water temperature decreases by 4-60С, compared with the optimal temperature, the structure of differential blood count, the content of red blood cells and white blood cells changes in catfish. The answer to the effect of temperature factor affected polymorphonuclear cells and monocytes in differential blood count, and to a lesser extent, lymphocytes. The other components of differential blood count did not change significantly. Significant changes occurred in red blood system. When the temperature decreased, the content of red blood cells in the blood significantly decreased. At the same time, the content of white blood cells significantly increased. Similar reactions of the blood system were observed with increased planting density. It was shown that the proportion of monocytes with increased planting density increased significantly. It is monocytes that carry out phagocytosis, which allows the body to fight naturally against the penetration of foreign invaders. It should be noted that the proportion of monocytes increased by more than for 76% against the background of increased planting density. This is obvious due to the fact that with a high planting density, the concentration of fish metabolites in water increases.. A high level of organic matter contributes to the development of pathogenic and opportunistic pathogenic microbiota that threatens the body of fish. It is this process that, in our opinion, stimulates mechanism of increased production of monocytes.

Organosomatic indices and leukocyte formula of chub (Squalius cephalus L) from the Suturlija River

Coefficients of organs, along with haematological characteristics, represent an important means of monitoring the health and condition of fish. Organosomatic indices show the state of organ systems and individual organs. They manifest as changes in size, which are reflected through a decrease or increase, under the influence of environmental factors. Chub individuals from the Suturlija River were used for analysis. The paper analyses the Fulton's condition factor, heart, liver, and spleen coefficients as a condition factor. Furthermore, the values ​​of total length, standard length, and body weight were determined for all individuals. Also, certain haematological parameters were analysed in the paper: total leukocyte count and differential blood count. A total of 19 chubs caught from the Suturlija River were analysed. The obtained result of Fulton's condition factor (1.53) indicates favourable habitats for chub individuals in the Suturlija River. The results of organosomatic indices are without major variations between individuals (CSI = 0.184, SSI = 0.992, HSI = 0.133). The mean leukocyte count was 45.857x109/l, with the highest proportion of lymphocytes in the differential blood count.

Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood

Blood is arguably the most important bodily fluid and its analysis provides crucial health status information. A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. What is lacking to advance initial blood diagnostics is an unbiased and quick functional assessment of blood that can narrow down the diagnosis and generate specific hypotheses. To address this need, we introduce the continuous, cell-by-cell morpho-rheological (MORE) analysis of diluted whole blood, without labeling, enrichment or separation, at rates of 1000 cells/sec. In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. This approach takes previous results of mechanical studies on specifically isolated blood cells to the level of application directly in blood and adds a functional dimension to conventional blood analysis.

Detection of human disease conditions by single-cell morpho-rheological phenotyping of whole blood

Blood is arguably the most important bodily fluid and its analysis provides crucial health status information. A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. What is lacking to advance initial blood diagnostics is an unbiased and quick functional assessment of blood that can narrow down the diagnosis and generate specific hypotheses. To address this need, we introduce the continuous, cell-by-cell morpho-rheological (MORE) analysis of whole blood, without labeling, enrichment or separation, at rates of 1,000 cells/sec. In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. This approach takes previous results of mechanical studies on specifically isolated blood cells to the level of application directly in whole blood and adds a functional dimension to conventional blood analysis.

PREGLED NAJPOGOSTEJŠIH NAPRAVILNOSTI V IZVIDU KRVNE SLIKE V URGENTNIH AMBULANTAH IN PREPOZNAVA URGENTNIH STANJ

Complete blood count is basic investigation in medicine. It gives us necessary information for acutely sick patient's diagnostic and treatment. We describe the most frequent etiology of red, white and thrombocyte cell lines abnormalities and give notice on conditions who require immediate measures.We had analyzed 1297 complete blood count results of patients who sought medical help in Emergency setting of Ljubljana's health care center from 1.1. 2014 to 31.1. 2014.Every fourth patient treated in the emergency setting had abnormalities in complete blood count. The most frequent finding was leucocytosis, following normocyte anemia and erytrocite's morphological abnormalities without laboratory signs of anemia. Every tenth patient had abnormalities in two or three cell lines.Complete blood count is probably the most basic investigation in medicine. From the results we can suspect on many different pathologies. Differential blood count should be ordered if we find abnormalities in complete blood count. Physicians should read and evaluate complete blood count with the same dedication as we read electrocardiogram.