Factors Affecting Zoo Visitors’ Conservation Beliefs and Knowledge of Large Carnivores in 2009 and a Dozen Years Later

Teaching in a formal learning environment mainly focuses on gaining knowledge, and scarcely on the development of pro-environmental attitudes. Knowledge can also be gained in informal learning institutions, such as zoos, and their potential use in general public education should not be neglected. This paper explores factors influencing the conservation beliefs of zoo visitors about brown bears, grey wolves, and Eurasian lynx. The study undertaken in Zoo Ljubljana (Slovenia) consisted of surveys performed in 2009 (n = 613) and in 2021 (n = 257). The levels of knowledge and education influenced both supporting and opposing beliefs about the three large carnivore species. The gender factor was less uniform: both supporting and opposing beliefs about lynx were demonstrated, but only opposing beliefs about brown bear and wolf. The study indicates that knowledge has the most significant influence on conservation beliefs, thus highlighting the importance of educational and communication activities in management and conservation actions regarding large carnivore species. The varied gender influence suggests that species-specific educational activities should be encouraged.

Multi-year Use of the Standard Gauge Railway Underpasses by Mammals in Tsavo, Kenya: Influence of Underpass Dimensions, Infrastructure and Ecology

Rail and road infrastructure is essential for economic growth and development but can cause a gradual loss in biodiversity and degradation of ecosystem function and services. We assessed influence of underpass dimensions, fencing, proximity to water and roads, Normalized Difference Vegetation Index (NDVI), presence of other species and livestock on underpass use by large and medium-sized mammals. Results revealed hyenas and leopards used the underpasses more than expected whereas giraffes and antelopes used the underpasses less than expected. Generalized linear mixed effects models (GLMMs) revealed that underpass height influenced their use by wildlife, with several species preferring to use taller underpasses. Electric fencing increased underpass use by funneling species towards underpasses, except for elephants and black-backed jackal for which it reduced underpass passage. GLMMs also revealed that the use of underpasses by livestock reduced the probability of their use by nearly 50% of wildlife species. Carnivore species were more likely to cross underpasses used by their prey. Buffalo, livestock, and hyenas used underpasses with higher NDVI and near water sources while baboons, dik-diks and antelope avoided underpasses with high NDVI. The findings suggest a need for diverse, and comprehensive approach for mitigating the negative impacts of rail on African wildlife.

A Unique Case of Fatal Coinfection Caused by Leptospira spp. and Hepatozoon canis in a Red Fox Cub (Vulpes vulpes)

Red foxes are the most abundant wild carnivore species in Europe commonly exposed to pathogenic Leptospira and Hepatozoon canis. Despite high seroprevalence, the clinical disease caused by these pathogens in red foxes has never been reported. Herein, we report the first-ever case of a fatal Leptospira spp. and H. canis coinfection in a two-month-old red fox cub with acute haemolytic anaemia, mild bronchopneumonia, intraalveolar haemorrhage, and tubulonephrosis. The presence of pathogenic Leptospira spp. DNA was detected in the kidney and lung tissues of the infected animal. In contrast to our previous knowledge, we believe that such fatal cases due to concomitant infection by Leptospira spp. and H. canis, especially in young animals, may commonly occur in nature. However, further studies are required to identify other factors that possibly contribute to the severity and the pathogenic effect of Leptospira spp. and H. canis infections in red foxes.

A seasonal pulse of ungulate neonates influences space use by carnivores in a multi-predator, multi-prey system

The behavioral mechanisms by which predators encounter prey are poorly resolved. In particular, the extent to which predators engage in active search for prey versus incidentally encountering them is unknown. The distinction between search and incidental encounter influences prey population dynamics with active search exerting a stabilizing force on prey populations by alleviating predation pressure on low-density prey and increasing it for high26 density prey. Parturition of many large herbivores occurs during a short and predictable temporal window in which young are highly vulnerable to predation. Our study aims to determine how a suite of carnivores responds to the seasonal pulse of newborn ungulates using contemporaneous GPS locations of four species of predators and two species of prey. We used step-selection functions to assess whether coyotes, cougars, black bears, and bobcats actively searched for parturient females in a low-density population of mule deer and a high-density population of elk. We then assessed whether searching carnivores shifted their habitat use toward areas exhibiting a high probability of encountering neonates. None of the four carnivore species encountered parturient mule deer more often than expected by chance suggesting that predation of young resulted from incidental encounters. By contrast, we determined that cougar and male bear movements positioned them in proximity of parturient elk more often than expected by chance which is evidence of searching behavior. Although both male bears and cougars searched for neonates, only male bears used elk parturition habitat in a way that dynamically tracked the phenology of the elk birth pulse suggesting that maximizing encounters with juvenile elk was a motivation when selecting resources. Our results support the existence of a stabilizing mechanism to prey populations through active search behavior by predators because carnivores in our study searched for the high45 density prey species (elk) but ignored the low-density species (mule deer). We conclude that prey density must be high enough to warrant active search, and that there is high interspecific and intersexual variability in foraging strategies among large mammalian predators and their prey.

Zoonotic Genotypes of Enterocytozoon bieneusi in Wild Living Invasive and Native Carnivores in Poland

Wild carnivores, both introduced and native species, are able to adapt well to peri-urban environments, facilitating cross-species pathogen transmission with domestic animals, and potentially humans. The role of wild living reservoir hosts cannot be ignored because of their known carriage of E. bieneusi zoonotic genotypes. In the past decades, populations of wild living carnivores, i.e., native, such as red foxes, and invasive, such as raccoon dogs and raccoons, have increased and adapted to synanthropic environments across Europe, including Poland. The knowledge concerning E. bieneusi genotype identification and distribution in wild carnivores is limited worldwide. A total of 322 individual fecal samples from six carnivore species, i.e., raccoon, raccoon dog, red fox, European badger, pine and beech martens, were collected and then analysed for the presence of E. bieneusi using the nested PCR method. Overall prevalence of the pathogen was estimated to be as high as 27.3%. The infection rates for E. bieneusi varied between the carnivore species, from 13.7% in beech martens to 40.4% in raccoon dogs. Based on sequence analysis of the ITS region of the rRNA gene marker, we detected five known genotypes of E. bieneusi in examined animals. In the invasive species, E. bieneusi NCF2 and D genotypes have been identified, whereas in the native ones, E. bieneusi NCF2, D, C, EbCar2 and Type IV genotypes were identified. All E. bieneusi genotypes recorded in this survey clustered in Group 1, showing their zoonotic potential. Our results provide the first description of the occurrence and genotypes of the microsporidian E. bieneusi in wild living population of raccoon dogs in Europe. Our findings are important for the study of pathogen epidemiology and emphasize the fact that the invasive and the native wild living carnivores, both widely distributed, should be considered more seriously as significant sources of zoonotic pathogens hazardous to domestic and farmed animals and humans.

The case for case studies: A new approach to evaluating the effectiveness of livestock protection tools

Livestock operations in California face livestock losses due to a range of carnivore species. Simultaneously, there is an increased call to reduce the use of lethal predator control methods and replace them with nonlethal methods. Livestock guardian dogs (Canis lupus familiaris) are one such nonlethal livestock protection tool (LPT), yet research is still lacking on the factors and situations that impact their effectiveness. Using three case studies, we demonstrate the value of objective analyses that explicitly address the inherent differences in ranch management, environment, and surrounding land uses in examining livestock guardian dogs as an LPT. We used semi-structured questionnaire surveys of livestock operators to collect information on effectiveness, behavior, and producer satisfaction of LGDs protecting poultry (Gallus gallus domesticus), calves (Bovus taurus), and sheep (Ovis aries) on private and public land and in conjunction with a variety of other livestock protection tools. We aimed to address all aspects related to the use of LGDs as a means of informing livestock operators’ decisions on whether LGDs are an appropriate tool for a particular operation. The case studies demonstrated the complexities involved in applying LGDs as a LPT within the context of a livestock operation. In two of the three case studies, LGDs did not entirely eliminate livestock losses yet operator satisfaction remained high.

Animal lifestyle affects acceptable mass limits for attached tags

Animal-attached devices have transformed our understanding of vertebrate ecology. To minimize any associated harm, researchers have long advocated that tag masses should not exceed 3% of carrier body mass. However, this ignores tag forces resulting from animal movement. Using data from collar-attached accelerometers on 10 diverse free-ranging terrestrial species from koalas to cheetahs, we detail a tag-based acceleration method to clarify acceptable tag mass limits. We quantify animal athleticism in terms of fractions of animal movement time devoted to different collar-recorded accelerations and convert those accelerations to forces (acceleration × tag mass) to allow derivation of any defined force limits for specified fractions of any animal's active time. Specifying that tags should exert forces that are less than 3% of the gravitational force exerted on the animal's body for 95% of the time led to corrected tag masses that should constitute between 1.6% and 2.98% of carrier mass, depending on athleticism. Strikingly, in four carnivore species encompassing two orders of magnitude in mass ( ca 2–200 kg), forces exerted by ‘3%' tags were equivalent to 4–19% of carrier body mass during moving, with a maximum of 54% in a hunting cheetah. This fundamentally changes how acceptable tag mass limits should be determined by ethics bodies, irrespective of the force and time limits specified.

Eurasian lynx populations in Western Europe: What prospects for the next 50 years?

Persistence of viable populations may be uncertain for large carnivore species, especially for those established in human-dominated landscapes. Here, we studied the Eurasian lynx in Western Europe established in the Upper Rhine metapopulation (i.e., Jura, Vosges-Palatinian and Black Forest populations) and in the Alpine population. These populations are currently considered as endangered or critically endangered due to high anthropogenic mortality and isolation. We assessed lynx persistence over a 50-year time horizon by implementing a spatially-explicit individual-based model while accounting for road mortality and habitat selection. For the Alpine and Jura populations, forecasts showed a steady growth rapidly reaching a stabilization phase with high resident female occupancy, and a positive growth for the newly re-established Vosges-Palatinian population. Moreover, this population showed a difference in female occupancy between the northern part, where a recent reintroduction program was conducted in the Palatinate Forest, and the southern part. Only the group of individuals in the Black Forest had an irregular growth probably due to the small number of only male lynx at start of the simulation and poor connectivity to surrounding populations. Exchanges of individuals between populations were limited, and the Jura population played the role of a crossroad. Persistence of lynx in Western Europe seems likely on a large scale over the next 50 years. However, lynx persistence in the southern part of the Vosges-Palatinian population and in the Black Forest appears challenging without long-term conservation management.

Temporal activity patterns suggesting niche partitioning of sympatric carnivores in Borneo, Malaysia

To propose proper conservation measures and to elucidate coexistence mechanisms of sympatric carnivore species, we assessed temporal activity patterns of the sympatric carnivore species using 37,379 photos collected for more than 3 years at three study sites in Borneo. We categorized activity patterns of nine carnivore species (one bear, three civets, two felids, one skunk, one mustelid, one linsang) by calculating the photo-capturing proportions at each time period (day, night, twilight). We then evaluated temporal activity overlaps by calculating the overlap coefficients. We identified six nocturnal (three civets, one felid, one skunk, one linsang), two diurnal (one felid, one mustelid), and one cathemeral (bear) species. Temporal activity overlaps were high among the nocturnal species. The two felid species possessing morphological and ecological similarities exhibited clear temporal niche segregation, but the three civet species with similar morphology and ecology did not. Broad dietary breadth may compensate for the high temporal niche overlaps among the nocturnal species. Despite the high species richness of Bornean carnivores, almost half are threatened with extinction. By comparing individual radio-tracking and our data, we propose that a long-term study of at least 2 or 3 years is necessary to understand animals’ temporal activity patterns, especially for sun bears and civets, by camera-trapping and to establish effective protection measures.