Effects on Puberty of Nutrition-Mediated Endocrine Disruptors Employed in Agriculture

Pesticide residues are largely found in daily consumed food because of their extensive use in farming and their long half-life, which prolongs their presence in the environment. Many of these pesticides act as endocrine-disrupting chemicals after pre- or postnatal exposure, significantly affecting, among other things, the time of puberty onset, progression, and completion. In humans, precocious or delayed puberty, and early or delayed sexual maturation, may entail several negative long-term health implications. In this review, we summarize the current evidence on the impact of endocrine-disrupting pesticides upon the timing of the landmarks of female and male puberty in both animals (vaginal opening, first estrus, and balanopreputial separation) and humans (thelarche, menarche, gonadarche). Moreover, we explore the possible mechanisms of action of the reviewed endocrine-disrupting pesticides on the human reproductive system. Access to safe, healthy, and nutritious food is fundamental for the maintenance of health and wellbeing. Eliminating the presence of hazardous chemicals in largely consumed food products may increase their nutritional value and be proven beneficial for overall health. Consequently, understanding the effects of human exposure to hazardous endocrine-disrupting pesticides, and legislating against their circulation, are of major importance for the protection of health in vulnerable populations, such as children and adolescents.

Subseasonal prediction of the 2020 Great Barrier Reef and Coral Sea marine heatwave

The 2020 marine heatwave in the Great Barrier Reef and Coral Sea led to mass coral bleaching. Sea surface temperature anomalies reached +1.7°C for the whole of the Great Barrier Reef and Coral Sea and exceeded +2°C across broad regions (referenced to 1990-2012). The marine heatwave reached Category 2 (Strong) and warm anomalies peaked between mid-February and mid-March 2020. The marine heatwave’s peak intensity aligned with regions of reduced cloud cover and weak wind speeds. We used a marine heatwave framework to assess the ability of an operational coupled ocean-atmosphere prediction system (ACCESS-S1) to capture the marine heatwave’s severity, duration, and spatial extent. For initial week predictions, the predicted marine heatwave severity generally agreed with the magnitude and spatial extent of the observed severity for that week. The model ensemble mean did not capture the marine heatwave’s development phase at lead times beyond the first week. The model underestimated the marine heatwave’s spatial extent, which reached up to 95% of the study area with at least Moderate severity and up to 43% with at least Strong severity. However, most forecast ensemble members correctly predicted the period of Strong severity in the first week of the model forecast. The model correctly predicted marine heatwave conditions to persist from mid-February to mid-March but did not capture the end of the marine heatwave. The inability to predict the end of the event and other periods of less skilful prediction were related to subseasonal variability owing to weather systems, including the passage of tropical cyclones not simulated in the model. On subseasonal timescales, evaluating daily to weekly forecasts of ocean temperature extremes is an important step toward implementing methods for developing operational forecast extremes products for use in early warning systems.

FASTAFS: file system virtualisation of random access compressed FASTA files

Background The FASTA file format, used to store polymeric sequence data, has become a bioinformatics file standard used for decades. The relatively large files require additional files, beyond the scope of the original format, to identify sequences and to provide random access. Multiple compressors have been developed to archive FASTA files back and forth, but these lack direct access to targeted content or metadata of the archive. Moreover, these solutions are not directly backwards compatible to FASTA files, resulting in limited software integration. Results We designed a linux based toolkit that virtualises the content of DNA, RNA and protein FASTA archives into the filesystem by using filesystem in userspace. This guarantees in-sync virtualised metadata files and offers fast random-access decompression using bit encodings plus Zstandard (zstd). The toolkit, FASTAFS, can track all its system-wide running instances, allows file integrity verification and can provide, instantly, scriptable access to sequence files and is easy to use and deploy. The file compression ratios were comparable but not superior to other state of the art archival tools, despite the innovative random access feature implemented in FASTAFS. Conclusions FASTAFS is a user-friendly and easy to deploy backwards compatible generic purpose solution to store and access compressed FASTA files, since it offers file system access to FASTA files as well as in-sync metadata files through file virtualisation. Using virtual filesystems as in-between layer offers format conversion without the need to rewrite code into different programming languages while preserving compatibility.

Denmark

The Danish Parliament, the Folketing, is elected through a highly proportional electoral system, which places a substantial value on personal votes. Despite this, access to the floor is not controlled by the party leadership, but rather party spokespersons are privileged. The average size of parliamentary party groups is relatively small, which limits the need for hierarchy to coordinate and restricts the ability of party leaders to prevent some MPs from taking the floor. Analyzing speeches given from the start of the 2011–2015 parliamentary term to the end of March 2020, it is found that female MPs are consistently under-represented in terms of parliamentary speeches in the Danish Folketing, and MPs from smaller parliamentary party groups speak significantly more often. No significant effects on the number of words spoken are found. Despite the highly personalized electoral system, access to the floor is neither influenced by electoral vulnerability nor by electoral success.

EP.TH.341Creating and testing the online multimedia platform aboutmyop.org

Introduction The value of patient focused registries and the use of patient reported outcomes is well documented. However, despite the availability of technological advances the use of multimedia interfaces with patient interaction and real time digitalised data collection has yet to become routine practice. As a modern-day solution, aboutmyop.org was developed as a patient-facing website to improve patient-clinician communication and information sharing. The aim of this study was to pilot the platform in routine clinical care, to identify acceptability, strength and weaknesses. Method Patients identified with symptomatic gallstones and referred for consideration for elective laparoscopic cholecystectomy were invited to use aboutmyop.org by postal invitation. Patient demographics, and information on patient recruitment were collected prospectively. Results The process of approvals to implement and integrate aboutmyop.org with NHS systems took over 18 months. Due to difficulties with onboarding, 349 (38.9%) of the 898 eligible patients registered, and fewer continued to participate post-operatively (7 days: 46.5%, 30-days: 30.5%, 3-months: 27%, 6-months: 19%). Participants were significantly younger than non-participants (47.6 vs. 51.8 years, p < 0.05), but were otherwise equally matched for gender and comorbidities. Despite system and implementation issues, participants were accepting and supportive of aboutmyop.org. Conclusion The aboutmyop.org system demonstrates a method to improve information accessibility for patients, and a novel method for data-sharing between patients and clinicians. Despite difficulties with system access, users demonstrated a willingness to engage in virtual recruitment and digital surveys. Subsequent improvements in access to a aboutmyop.org should help to facilitate long-term patient engagement and quantify real world benefits.

The management of dialysis access thrombosis during the COVID-19 pandemic

Background: Maintaining patent access is essential for haemodialysis dependent end stage renal failure patients. The COVID-19 pandemic has significantly affected surgical and interventional radiology services worldwide. We aimed to review the impact COVID-19 has caused to the management of acute dialysis access thrombosis. Methods: We conducted a single centre retrospective review of outcomes of patients with arteriovenous fistula and arteriovenous graft thrombosis between March and May 2020, which coincided with the first peak of the COVID-19 pandemic in London, and a similar period in the previous year, March–May 2019. Outcomes in both cohorts of patients were compared, including attempts at salvage, salvage success, 1-month patency rates after salvage and subsequent surgery on the same access. We also analysed the use of tunnelled haemodialysis lines (THL), either due to failed salvage attempts or when salvage was not attempted. Results: There was a similar incidence of access thrombosis in both periods (26 cases in 2019, 38 in 2020). There were 601 patients dialysing via an arteriovenous fistula or graft in 2019, and 568 patients in 2020. Access salvage, when attempted, had similar success rates and 1-month patency (salvage success 74% vs 80%, p = 0.39; 1-month patency 55% vs 62%, p = 0.69). The proportion of patients where access salvage was not attempted and a THL inserted was significantly higher in 2020 compared to 2019 (32% vs 4%, p = 0.007). There were more patients who subsequently had surgery to salvage or revise the same access in 2019 compared to 2020 (62% vs 13%, p < 0.001). Conclusions: During the peak of the COVID-19 pandemic, there were fewer attempts at access salvage. This was a conscious decision due to increased pressure on the healthcare system, access to emergency interventional radiology or operative theatres and the perceived risk/benefit ratio of access salvage. The long-term effects of this change in practice remain unknown.

Which hip morphology measures and patient factors are associated with age of onset and symptom severity in femoroacetabular impingement syndrome?

Background: Bony morphology is central to the pathomechanism of femoroacetabular impingement syndrome (FAIS), however isolated radiographic measures poorly predict symptom onset and severity. More comprehensive morphology measurement considered together with patient factors may better predict symptom presentation. This study aimed to determine the morphological parameter(s) and patient factor(s) associated with symptom age of onset and severity in FAIS. Methods: 99 participants (age 32.9 ± 10.5 years; body mass index (BMI 24.3 ± 3.1 kg/m2; 42% females) diagnosed with FAIS received standardised plain radiographs and magnetic resonance scans. Alpha angle in four radial planes (superior to anterior), acetabular version (AV), femoral torsion, lateral centre-edge, anterior centre-edge (ACEA) and femoral neck-shaft angles were measured. Age of symptom onset (age at presentation minus duration of symptoms), international Hip Outcome Tool-33 (iHOT-33) and modified UCLA activity scores were recorded. Backward stepwise regression assessed morphological parameters and patient factors (age, sex, BMI, symptom duration, annual income, private/public healthcare system accessed) to determine variables independently associated with onset age and iHOT-33 score. Results: Earlier symptom onset was associated with larger superoanterior alpha angle ( p = 0.007), smaller AV ( p = 0.023), lower BMI ( p = 0.010) and public healthcare system access ( p = 0.041) (r2 = 0.320). Worse iHOT-33 score was associated with smaller ACEA ( p = 0.034), female sex ( p = 0.040), worse modified UCLA activity score ( p = 0.010) and public healthcare system access ( p < 0.001) (r2 = 0.340). Conclusions: Age of symptom onset was chiefly predicted by femoral and acetabular bony morphology measures, whereas symptom severity predominantly by patient factors. Factors measured explained a small amount of variance in the data; additional unmeasured factors may be more influential.

Modern conditions of functioning of construction companies of Odessa

The purpose of the study conducted in the article is to consider current trends in the operation and development of construction companies in Odessa. Groups of developers in this market, areas of their specialization are analyzed. The main attention is paid to the competitive advantages of industry leaders. Factors such as the level of costs, purchase prices for building materials, credit, quality of construction, the availability of a strategic planning system, access to the most liquid areas of construction, high advertising costs, the company’s image among consumers. The high level of dependence of construction companies on environmental factors requires special attention to the risks associated with changes in the external business environment, which can significantly affect the change in the structure of the construction industry in the long run.

EngraveChain: A Blockchain-Based Tamper-Proof Distributed Log System

A reliable log system is a prerequisite for many applications. Financial systems need to have transactions logged in a precise manner, medical systems rely on having trusted medical records and security logs record system access requests in order to trace malicious attempts. Keeping multiple copies helps to achieve availability and reliability against such hackers. Unfortunately, maintaining redundant copies in a distributed manner in a byzantine setting has always been a challenging task, however it has recently become simpler given advances in blockchain technologies. In this work, we present a tamper-resistant log system through the use of a blockchain. We leverage the immutable write action and distributed storage provided by the blockchain as a basis to develop a secure log system, but we also add a privacy preserving layer that is essential for many applications. We detail the security and privacy aspects of our solution, as well as how they relate to performance needs in relevant settings. Finally, we implement our system over Hyperledger Fabric and demonstrate the system’s value for several use cases. In addition, we provide a scalability analysis for applying our solution in a large-scale system.