ZC3HC1 is a structural element of the nuclear basket effecting interlinkage of TPR polypeptides

The nuclear basket (NB), anchored to the nuclear pore complex (NPC), is commonly thought of as built solely of protein TPR polypeptides, the latter thus regarded as the NB's only scaffold-forming components. In the current study, we report ZC3HC1 as a second building element of the NB. Recently described as an NB-appended protein omnipresent in vertebrates, we now show that ZC3HC1, both in vivo and in vitro, enables in a step-wise fashion the recruitment of TPR subpopulations to the NB and their linkage to already NPC-anchored TPR polypeptides. We further demonstrate that the degron-mediated rapid elimination of ZC3HC1 results in the prompt detachment of the ZC3HC1-appended TPR polypeptides from the NB and their release back into the nucleoplasm again, underscoring the role of ZC3HC1 as a natural structural element of the NB. Finally, we show that ZC3HC1 can keep TPR polypeptides positioned even at sites remote from the NB, in line with ZC3HC1 functioning as a protein connecting TPR polypeptides.

EXTRACORPOREAL DETOXIFICATION METHODS IN THE PATHOGENETIC THERAPY OF OBSTETRIC SEPSIS

Раннее включение НВВГФ в комплексном лечении сепсиса и септического шока приводит к снижению клинических признаков эндотоксикоза, стабилизации гемодинамики в более ранние сроки, что связано с быстрой элиминацией цитокинов, коррекцией осмотического равновесия, увеличением диуреза, благодаря более раннему протезированию гомеостатической функции почек. Early inclusion of NVHF in the complex treatment of sepsis and septic shock leads to a decrease in clinical signs of endotoxicosis, stabilization of hemodynamics at an earlier time, which is associated with rapid elimination of cytokines, correction of osmotic balance, increased urine output due to earlier prosthetics of homeostatic renal function.

Combined Effects of Temperature and Toxic Algal Abundance on Paralytic Shellfish Toxic Accumulation, Tissue Distribution and Elimination Dynamics in Mussels Mytilus coruscus

This study assessed the impact of increasing seawater surface temperature (SST) and toxic algal abundance (TAA) on the accumulation, tissue distribution and elimination dynamics of paralytic shellfish toxins (PSTs) in mussels. Mytilus coruscus were fed with the PSTs-producing dinoflagellate A. catenella under four simulated environment conditions. The maximum PSTs concentration was determined to be 3548 µg STX eq.kg−1, which was four times higher than the EU regulatory limit. The increasing SST caused a significant decline in PSTs levels in mussels with rapid elimination rates, whereas high TAA increased the PSTs concentration. As a result, the PSTs toxicity levels decreased under the combined condition. Additionally, toxin burdens were assessed within shellfish tissues, with the highest levels quantified in the hepatopancreas. It is noteworthy that the toxin burden shifted towards the mantle from gill, muscle and gonad at the 17th day. Moreover, variability of PSTs was measured, and was associated with changes in each environmental factor. Hence, this study primarily illustrates the combined effects of SST and TAA on PSTs toxicity, showing that increasing environmental temperature is of benefit to lower PSTs toxicity with rapid elimination rates.

Gastroesophageal reflux disease in paramedic practice

Gastroesophageal reflux disease is a condition that develops when reflux of stomach contents causes disturbing symptoms and / or complications. Its typical symptoms are heartburn and regurgitation (belching sour, bitter, stomach contents), which worsen the well-being of patients. One of the main tasks of medical treatment is the rapid elimination and control of symptoms. Complications of GERD include reflux esophagitis, Barrett’s esophagus, and esophageal adenocarcinoma. This article highlights the use of alginates in the treatment of gastroesophageal reflux disease (GERD) in accordance with modern concepts of this condition and approaches to its treatment.