Simulation and Prediction of Fungal Community Evolution Based on RBF Neural Network

Simulation and prediction of the scale change of fungal community. First, using the experimental data of a variety of fungal decomposition activities, a mathematical model of the decomposition rate and the relationship between the bacterial species was established, thereby revealing the internal mechanism of fungal decomposition activity in a complex environment. Second, based on the linear regression method and the principle of biodiversity, a model of fungal decomposition rate was constructed, and it was concluded that the interaction between mycelial elongation and moisture resistance could increase the fungal decomposition rate. Third, the differential equations are used to quantify the competitive relationship between different bacterial species, divide the boundaries of superior and inferior species, and simulate the long-term and short-term evolution trends of the community under the same initial environment. And an empirical analysis is made by taking the sudden change of the atmosphere affecting the evolution of the colony as an example. Finally, starting from summer, combining soil temperature, humidity, and fungal species data in five different environments such as arid and semiarid, a three-dimensional model and RBF neural network are introduced to predict community evolution. The study concluded that under given conditions, different strains are in short-term competition, and in the long-term, mutually beneficial symbiosis. Biodiversity is important for the biological regulation of nature.

Revaluation of agri-food waste to obtain bioethanol

Objective: to produce bioethanol from the alcoholic fermentation of agri-food waste.Design/methodology/approach: food waste was collected for one month and separated into fruit and fabaceous waste; its size was reduced and then washed with hot acetone. A batch of 100 g of residue underwent acid hydrolysis with 5 %H 2 SO 4 at 125 °C, the hydrolysate was fermented with Saccharomyces cerevisiae at 30 °C for 48 h; the ferment was then distilled at 78 °C. The sugar content was determined following the phenol-sulfuric method. Brix degrees, density andpercentage w/w ethanol were measured with a densimeter.Results: from the fruit residues, an organic fraction was obtained with a total sugar content of 53.3 g/100 g of residue and 9.6 °Brix, generating 45 mL of distillate with 3.8 % w/w of bioethanol. From the fabaceae residues, an organic fraction was obtained with a total sugar content of 19.4 g and 4.140 °Brix, generating 30 mL of distillate with 2.54 % w/w of bioethanol.Study limitations/implications: Rapid decomposition of waste due to bacterial and fungal decomposition complicates long-term storage.Findings/conclusions: fermentable sugars can be obtained from the evaluated agri-food waste to obtain bioethanol. In this way, they can be integrated into the value chain as raw materials, reducing their accumulation and the environmental impact generated by their final disposal.

Establishment of Fungal Decomposition Model Based on OLS and Logistic Model

By using the OLS model, an equation for the rate of decomposing wood by a variety of fungi was established. We analyzed the effects of various fungi in the experimental data under different temperature and humidity. Based on the growth performance of different fungi at different temperatures and humidity, we use the method of systematic cluster to divide the fungi into 5 categories, and introduce competition levels as the viability of different species of fungi. We have established a logistic model that introduces competition levels to obtain a fungal habitat model. The fungal habitat model includes predictions about the relative advantages and disadvantages for each species and combinations of species likely to persist, and do so for different environments including arid, semi-arid, temperate, arboreal, and tropical rain forests.

Targeted acetylation of wood: a tool for tuning wood-water interactions

Wood is an increasingly important material in the sustainable transition of societies worldwide. The performance of wood in structures is intimately tied to the presence of moisture in the material, which directly affects important characteristics such as dimensions and mechanical properties, and indirectly its susceptibility to fungal decomposition. By chemical modification, the durability of wood in outdoor environments can be improved by reducing the amount of moisture present. In this study, we refined a well-known chemical modification with acetic anhydride and showed how the spatial distribution of the modification of Norway spruce (Picea abies (L.) Karst.) could be controlled with the aim of altering the wood-water interactions differently in different parts of the wood structure. By controlling the reaction conditions of the acetylation it was possible to acetylate only the cell wall-lumen interface, or uniformly modify the whole cell wall to different degrees. The spatial distribution of the acetylation was visualised by confocal Raman microspectroscopy. The results showed that by this targeted acetylation procedure it was possible to independently alter the wood-water interactions in and outside of cell walls. The cell wall-lumen interface modification altered the interaction between the wood and the water in cell lumina without affecting the interaction with water in cell walls while the uniform modification affected both. This opens up a novel path for studying wood-water interactions in very moist environments and how moisture distribution within the wood affects its susceptibility towards fungal decomposition. Graphic abstract

Regulation of fungal decomposition at single-cell level

Filamentous fungi play a key role as decomposers in Earth’s nutrient cycles. In soils, substrates are heterogeneously distributed in microenvironments. Hence, individual hyphae of a mycelium may experience very different environmental conditions simultaneously. In the current work, we investigated how fungi cope with local environmental variations at single-cell level. We developed a method based on infrared spectroscopy that allows the direct, in-situ chemical imaging of the decomposition activity of individual hyphal tips. Colonies of the ectomycorrhizal Basidiomycete Paxillus involutus were grown on liquid media, while parts of colonies were allowed to colonize lignin patches. Oxidative decomposition of lignin by individual hyphae growing under different conditions was followed for a period of seven days. We identified two sub-populations of hyphal tips: one with low decomposition activity and one with much higher activity. Active cells secreted more extracellular polymeric substances and oxidized lignin more strongly. The ratio of active to inactive hyphae strongly depended on the environmental conditions in lignin patches, but was further mediated by the decomposition activity of entire mycelia. Phenotypic heterogeneity occurring between genetically identical hyphal tips may be an important strategy for filamentous fungi to cope with heterogeneous and constantly changing soil environments.