Long-Time Behavior of Surface Properties of Microstructures Fabricated by Multiphoton Lithography

The multiphoton lithography (MPL) technique represents the future of 3D microprinting, enabling the production of complex microscale objects with high precision. Although the MPL fabrication parameters are widely evaluated and discussed, not much attention has been given to the microscopic properties of 3D objects with respect to their surface properties and time-dependent stability. These properties are of crucial importance when it comes to the safe and durable use of these structures in biomedical applications. In this work, we investigate the surface properties of the MPL-produced SZ2080 polymeric microstructures with regard to the physical aging processes during the post-production stage. The influence of aging on the polymeric microstructures was investigated by means of Atomic Force Microscopy (AFM) and X-ray Photoelectron Spectroscopy (XPS). As a result, a time-dependent change in Young’s Modulus, plastic deformation, and adhesion and their correlation to the development in chemical composition of the surface of MPL-microstructures are evaluated. The results presented here are valuable for the application of MPL-fabricated 3D objects in general, but especially in medical technology as they give detailed information of the physical and chemical time-dependent dynamic behavior of MPL-printed surfaces and thus their suitability and performance in biological systems.

Design and Characterization of Microscale Auxetic and Anisotropic Structures Fabricated by Multiphoton Lithography

The need for control of the elastic properties of architected materials has been accentuated due to the advances in modelling and characterization. Among the plethora of unconventional mechanical responses, controlled anisotropy and auxeticity have been promulgated as a new avenue in bioengineering applications. This paper aims to delineate the mechanical performance of characteristic auxetic and anisotropic designs fabricated by multiphoton lithography. Through finite element analysis the distinct responses of representative topologies are conveyed. In addition, nanoindentation experiments observed in-situ through scanning electron microscopy enable the validation of the modeling and the observation of the anisotropic or auxetic phenomena. Our results herald how these categories of architected materials can be investigated at the microscale.

A low-autofluorescence, transparent resin for multiphoton 3D printing

Multiphoton lithography allows the high resolution, free-form 3D printing of structures such as micro-optical elements and 3D scaffolds for Tissue Engineering. A major obstacle in its application in these fields is material and structure autofluorescence. Existing photoresists promise near zero fluorescent in expense of poor mechanical properties, and low printing efficiency. Sudan Black B is a molecular quencher used as a dye for biological studies and as means of decreasing the autofluorescence of polymers. In our study we report the use of Sudan Black B as both a photoinitiator and as a post-fabrication treatment step, using the zirconium silicate SZ2080TM for the development of a non-fluorescent composite. We use this material for the 3D printing of micro-optical elements, and meso-scale scaffolds for Mesenchymal Stem Cell cultures. Our results show the hybrid, made photosensitive with Sudan Black B, can be used for the fabrication of high resolution, highly transparent, autofluorescence-free microstructures.

Design and Testing of Bistable Lattices with Tensegrity Architecture and Nanoscale Features Fabricated by Multiphoton Lithography

A bistable response is an innate feature of tensegrity metamaterials, which is a conundrum to attain in other metamaterials, since it ushers unconventional static and dynamical mechanical behaviors. This paper investigates the design, modeling, fabrication and testing of bistable lattices with tensegrity architecture and nanoscale features. First, a method to design bistable lattices tessellating tensegrity units is formulated. The additive manufacturing of these structures is performed through multiphoton lithography, which enables the fabrication of microscale structures with nanoscale features and extremely high resolution. Different modular lattices, comprised of struts with 250 nm minimum radius, are tested under loading-unloading uniaxial compression nanoindentation tests. The compression tests confirmed the activation of the designed bistable twisting mechanism in the examined lattices, combined with a moderate viscoelastic response. The force-displacement plots of the 3D assemblies of bistable tensegrity prisms reveal a softening behavior during the loading from the primary stable configuration and a subsequent snapping event that drives the structure into a secondary stable configuration. The twisting mechanism that characterizes such a transition is preserved after unloading and during repeated loading-unloading cycles. The results of the present study elucidate that fabrication of multistable tensegrity lattices is highly feasible via multiphoton lithography and promulgates the fabrication of multi-cell tensegrity metamaterials with unprecedented static and dynamic responses.

3D multiphoton lithography using biocompatible polymers with specific mechanical properties

Two new biocompatible polymers were designed, which can be 3D structured via multiphoton lithography. Their mechanical properties and biocompatibility were determined.