Immunological detection of APP and proteins of the endolysosomal system v1

Here we present a general protocol for immunological detection by Western blotting of APP and proteins of the endolysosomal system, including EEA1, RAB5, PSEN1, LAMP1, LAMP2, TMEM192, and BACE1.

False immunological detection of CTX-M enzymes in Klebsiella oxytoca

The global spread of extended-spectrum-β-lactamase (ESBL) is a matter of great concern given high morbidity and mortality associated with invasive infections.…

Immunological detection of thymine dimers in indigenous genomic DNA from pre-disinfection drinking water as an ultraviolet disinfection dosimeter

A linear UV dose response is demonstrated using an ELISA assay that measures thymine dimers formed in the genomic DNA extracted from the mixed species of microorganisms filtered from UV treated pre-disinfection drinking water.

Functional nanomaterials based immunological detection of aflatoxin B1: a review

Aflatoxin B1 (AFB1) is highly carcinogenic, mutagenic and teratogenic. Accordingly, sensitive, rapid and cost-effective techniques for detection of AFB1 is in urgent demand for food safety and the health of consumers. In this review, we report the current state of immunoassay formats and development, mainly based on nanomaterials for determination of AFB1. Following an introduction of the field, the microplate-, membrane- and microelectrode-based immunoassays are described. The relevant mechanisms, sensitivities, superiorities and deficiencies of each format are discussed. Finally, perspectives on the future development of nanomaterials-based immunoassays for AFB1 are provided.