Mobile Biomonitoring of Atmospheric Pollution: A New Perspective for the Moss-Bag Approach

In this work the potential of moving moss-bags, fixed to bicycles, to intercept particulate matter (PM) and linked metal(loid)s was tested for the first time. Seven volunteers carried three moss-bags for fifty days while commuting by bicycle in the urban area of Antwerp, Belgium. Moreover, one bike, equipped with mobile PM samplers, travelled along four routes: urban, industrial, green route and the total path, carrying three moss-bags at each route. The saturation isothermal remanent magnetization (SIRM) signal and chemical composition (assessed by HR-ICP-MS) of the moss samples indicated that the industrial route was the most polluted. Element fluxes (i.e., the ratio between element daily uptake and the specific leaf area) could discriminate among land uses; particularly, they were significantly higher in the industrial route for Ag, As, Cd and Pb; significantly lowest in the green route for As and Pb; and comparable for all accumulated elements along most urban routes. A comparison with a previous experiment carried out in the same study area using similar moss-bags at static exposure points, showed that the element fluxes were significantly higher in the mobile system. Finally, PM2.5 and PM10 masses measured along the four routes were consistent with element fluxes.

Optimizing Water-Based Extraction of Bioactive Principles of Hawthorn: From Experimental Laboratory Research to Homemade Preparations

Hawthorn (Crataegus) is used for its cardiotonic, hypotensive, vasodilative, sedative, antiatherosclerotic, and antihyperlipidemic properties. One of the main goals of this work was to find a well-defined optimized extraction protocol usable by each of us that would lead to repeatable, controlled, and quantified daily uptake of active components from hawthorn at a drinkable temperature (below 60 °C). A thorough investigation of the extraction mode in water (infusion, maceration, percolation, ultrasounds, microwaves) on the yield of extraction and the amount of phenolic compounds, flavonoids, and proanthocyanidin oligomers as well as on the Ultra High Performance Liquid Chromatography (UHPLC) profiles of the extracted compounds was carried out. High-resolution Fourier transform ion cyclotron resonance mass spectrometry was also implemented to discriminate the different samples and conditions of extraction. The quantitative and qualitative aspects of the extraction as well as the kinetics of extraction were studied, not only according to the part (flowers or leaves), the state (fresh or dried), and the granulometry of the dry plant, but also the stirring speed, the temperature, the extraction time, the volume of the container (cup, mug or bowl) and the use of infusion bags.

Synthesis of deoxynivalenol-3-ß-D-O-glucuronide for its use as biomarker for dietary deoxynivalenol exposure

Trichothecene mycotoxins are prevalent toxic secondary metabolic products of several fungal species and pose a serious threat to human and animal health. Deoxynivalenol (DON) is known to undergo rapid metabolisation after uptake. The formed glucuronides are urinary excreted and could therefore serve as possible biomarkers for daily uptake measurement. So far human exposure to the major toxin DON was estimated from dietary average intake or by measurement of the parent toxin after hydrolysis. These approaches are indirect and time-consuming. Due to the clear demand for a direct determination method and lack of an available reference substance we synthesised DON-3-O-ö-D-glucuronide. The Königs-Knorr procedure using acetobromo-α-D-glucuronic acid methyl ester as glucuronyl-donor was optimised to produce the target compound in mg scale allowing subsequent characterisation via nuclear magnetic resonance and LC-MS/MS.

Improved sapflow methodology reveals considerable night-time ozone uptake by Mediterranean species

Due to the evident tropospheric ozone impact on plant productivity, an accurate ozone risk assessment for the vegetation has become an issue. There is a growing evidence that ozone stomatal uptake may also take place at night and that the night-time uptake may be more damaging than diurnal uptake. Estimation of night-time uptake in the field is complicated because of instrumental difficulties. Eddy covariance technology is not always reliable because of the low turbulence at night. Leaf level porometry is defective at relative humidity above 70% which often takes place at night. Improved sap flow technology allows to estimate also slow flows that usually take place at night and hence may be, at present, the most trustworthy technology to measure night-time transpiration and hence to derive canopy stomatal conductance and ozone uptake at night. Based on micrometeorological data and the sap flow of three Mediterranean woody species, the night-time ozone uptake of these species was evaluated during a summer season as drought increased. Night-time ozone uptake was from 10% to 18% of the total daily uptake when plants were exposed to a weak drought, but increased up to 24% as the drought became more pronounced. The percentage increase is due to a stronger reduction of diurnal stomatal conductance than night-time stomatal conductance.

Prophylactic Effect of Dietary Seaweed Fucoidan against Enteral Prion Infection

ABSTRACT Dietary seaweed fucoidan delays the onset of disease of enterally infected mice with scrapie when given orally for 6 days after infection, but not when given before the infection. This effect was not modified at a tested fucoidan dose range and appeared to reach the maximum level at a concentration of 2.5% or less in feed. Daily uptake of fucoidan might be prophylactic against prion diseases caused by ingestion of prion-contaminated materials, although further evaluation of its pharmacology remains to be done.