This paper describes the development and psychometric evaluation of a behavioral assessment instrument primarily intended for use with workgroups. The instrument is based on the Nurturing Environments framework which describes four domains important for health, well-being, and productivity; minimizing toxic social interactions, teaching and reinforcing prosocial behaviors, limiting opportunities for problem behaviors, and promoting psychological flexibility. In this article, questionnaire data of perceived frequency of behaviors relevant to nurturance are analyzed using both classic and modern test theory. The results indicate a 23-item instrument that best fit a bifactor model with a general nurturance factor explaining 87.1% of the variance in unit-weighted total scores, and three specific factors (toxic behavior, prosocial behavior, and behaviors that limit problems). Rasch analysis showed that the response scale works adequately, item fit is satisfactory, and no significant differential item functioning. Targeting is skewed towards lower levels of nurturance and item thresholds are distributed over the range of participant abilities. The instrument is freely available to use and adapt under a CC-BY license and intended as a tool that is easy for any group to use and interpret to identify key behaviors to improve their psychosocial work environment. By using a two-dimensional assessment with ratings of both frequency and perceived importance of behaviors the instrument can help facilitate a participatory group development process. We provide recommendations for how to work with a group based on their data, and how to optimize the measurement precision further. The next steps in research are suggested, such as group-level analysis, collecting observational data, and validation against concrete longitudinal outcomes. This instrument could help promote transparent assessment practices in organizational and group development.